scholarly journals A close analysis of metal-enhanced fluorescence of tryptophan induced by silver nanoparticles: wavelength emission dependence

2013 ◽  
Vol 11 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Anderson Caires ◽  
Luciano Costa ◽  
Joelson Fernandes
Keyword(s):  
Silver Nanoparticles ◽  
Inner Filter Effect ◽  
Label Free ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Promising Alternative ◽  
Close Analysis ◽  
Label Free Detection ◽  
Nanoparticles Concentration ◽  
Wavelength Emission

AbstractIn the last few years, silver nanoparticles have been proposed as a promising alternative for the label-free detection of proteins via metal-enhanced fluorescence. Generally, the aromatic amino acid tryptophan is most frequently used in this type of studies, because the intrinsic fluorescence of proteins is usually dominated by tryptophan emissions. In the present study, we evaluated the fluorescence behavior of tryptophan in the presence of a silver colloid with nanoparticles of 100 nm in diameter. The results showed that a nanoparticles concentration of 32 mg L−1 induced maximum fluorescence enhancement. However, the metal-enhanced fluorescence was dependent on the emission wavelength of tryptophan, and this phenomenon was closely related to the metal surface reabsorption process (inner filter effect), suggesting that the plasmon resonance reabsorption effect should be taken into account in analyses involving protein studies by metal-enhanced fluorescence.

scholarly journals Aluminum Nanoparticles as Substrates for Metal-Enhanced Fluorescence in the Ultraviolet for the Label-Free Detection of Biomolecules

2009 ◽  
Vol 81 (4) ◽  
pp. 1397-1403 ◽  
Author(s):  
Mustafa H. Chowdhury ◽  
Krishanu Ray ◽  
Stephen K. Gray ◽  
James Pond ◽  
Joseph R. Lakowicz
Keyword(s):  
Aluminum Nanoparticles ◽  
Label Free ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Label Free Detection ◽  
Detection Of Biomolecules

Photonic crystals: A platform for label-free and enhanced fluorescence biomolecular and cellular assays

2008 ◽  
Vol 1133 ◽  
Author(s):  
Brian T. Cunningham ◽  
Leo Chan ◽  
Patrick C. Mathias ◽  
Nikhil Ganesh ◽  
Sherine George ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Photonic Crystals ◽  
High Throughput Screening ◽  
High Sensitivity ◽  
Excitation Wavelength ◽  
Label Free ◽  
Crystal Surfaces ◽  
Enhanced Fluorescence ◽  
Preferred Direction ◽  
Label Free Detection

Abstract Photonic crystal surfaces represent a class of resonant optical structures that are capable of supporting high intensity electromagnetic standing waves with near-field and far-field properties that can be exploited for high sensitivity detection of biomolecules and cells. While modulation of the resonant wavelength of a photonic crystal by the dielectric permittivity of adsorbed biomaterials enables label-free detection, the resonance can also be tuned to coincide with the excitation wavelength of common fluorescent tags - including organic molecules and semiconductor quantum dots. Photonic crystals are also capable of efficiently channeling fluorescent emission into a preferred direction for enhanced extraction efficiency. Photonic crystals can be designed to support multiple resonant modes that can perform label free detection, enhanced fluorescence excitation, and enhanced fluorescence extraction simultaneously on the same device. Because photonic crystal surfaces may be inexpensively produced over large surface areas by nanoreplica molding processes, they can be incorporated into disposable labware for applications such as pharmaceutical high throughput screening. In this talk, the optical properties of surface photonic crystals will be reviewed and several applications will be described, including results from screening a 200,000-member chemical compound library for inhibitors of protein-DNA interactions, gene expression microarrays, and high sensitivity of protein biomarkers.

Fabrication and Metal-enhanced Fluorescence of Plasmonic Hybrid Nanotubes Consisting of Polythiophene and Silver Nanoparticles

2015 ◽  
Vol 44 (2) ◽  
pp. 135-137 ◽  
Author(s):  
Ryusuke Yoshida ◽  
Takuya Matsumura ◽  
Tsukasa Nakahodo ◽  
Hisashi Fujihara
Keyword(s):  
Silver Nanoparticles ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence

Silver Nanoparticles with Enhanced Fluorescence Effects on Fluorescein Derivative

2012 ◽  
Vol 602-604 ◽  
pp. 187-191 ◽  
Author(s):  
Chun Yang ◽  
Feng Yan Ge ◽  
Jin Cai Li ◽  
Zai Sheng Cai ◽  
Fang Fang Qin
Keyword(s):  
Silver Nanoparticles ◽  
Reduction Method ◽  
Fluorescent Dyes ◽  
Ph Value ◽  
Fold Increase ◽  
Borohydride Reduction ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Fluorescence Efficiency ◽  
Sodium Borohydride Reduction

Silver nanoparticles were prepared by sodium borohydride reduction method for analyzing metal-enhanced fluorescence property. Some variables including the dosage of reagent, reacting temperature and pH value had been investigated. Subsequently, a comparison of metal-enhanced fluorescence efficiency was made between two kinds of fluorescent dyes, namely fluorescein and 6-carboxyfluorescein at different silver concentrations. The experimental results show that the fluorescence of both dyes are remarkably enhanced. It is interesting to note that the increase of emission intensity is stronger than that of their corresponding excitation ones. Furthermore, a 5.038-fold increase in fluorescence for 6-carboxyfluorescein while a 2.506-fold increase for fluorescein are observed. This may attribute to the interaction between dyes and silver nanoparticels.

Ag@SiO2-entrapped hydrogel microarray: a new platform for a metal-enhanced fluorescence-based protein assay

The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3375-3383 ◽  
Author(s):  
Eunji Jang ◽  
Minsu Kim ◽  
Won-Gun Koh
Keyword(s):  
Silver Nanoparticles ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Protein Assay ◽  
Poly Ethylene

We developed a novel silver-based metal-enhanced fluorescence (MEF) biosensing platform that consisted of poly(ethylene glycol)(PEG) hydrogel microstructures entrapping silica-coated silver nanoparticles (Ag@SiO2).

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