Silver Nanoparticles with Enhanced Fluorescence Effects on Fluorescein Derivative

2012 ◽  
Vol 602-604 ◽  
pp. 187-191 ◽  
Author(s):  
Chun Yang ◽  
Feng Yan Ge ◽  
Jin Cai Li ◽  
Zai Sheng Cai ◽  
Fang Fang Qin
Keyword(s):  
Silver Nanoparticles ◽  
Reduction Method ◽  
Fluorescent Dyes ◽  
Ph Value ◽  
Fold Increase ◽  
Borohydride Reduction ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Fluorescence Efficiency ◽  
Sodium Borohydride Reduction

Silver nanoparticles were prepared by sodium borohydride reduction method for analyzing metal-enhanced fluorescence property. Some variables including the dosage of reagent, reacting temperature and pH value had been investigated. Subsequently, a comparison of metal-enhanced fluorescence efficiency was made between two kinds of fluorescent dyes, namely fluorescein and 6-carboxyfluorescein at different silver concentrations. The experimental results show that the fluorescence of both dyes are remarkably enhanced. It is interesting to note that the increase of emission intensity is stronger than that of their corresponding excitation ones. Furthermore, a 5.038-fold increase in fluorescence for 6-carboxyfluorescein while a 2.506-fold increase for fluorescein are observed. This may attribute to the interaction between dyes and silver nanoparticels.

Fabrication and Metal-enhanced Fluorescence of Plasmonic Hybrid Nanotubes Consisting of Polythiophene and Silver Nanoparticles

2015 ◽  
Vol 44 (2) ◽  
pp. 135-137 ◽  
Author(s):  
Ryusuke Yoshida ◽  
Takuya Matsumura ◽  
Tsukasa Nakahodo ◽  
Hisashi Fujihara
Keyword(s):  
Silver Nanoparticles ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence

scholarly journals Nanosilver against fungi. Silver nanoparticles as an effective biocidal factor.

1970 ◽  
Vol 60 (4) ◽  
Author(s):  
Jolanta Pulit ◽  
Marcin Banach ◽  
Renata Szczygłowska ◽  
Mirosław Bryk
Keyword(s):  
Silver Nanoparticles ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Reduction Method ◽  
Ph Value ◽  
Chemical Reduction ◽  
Silver Ions ◽  
Chemical Reduction Method ◽  
Stabilizing Agents ◽  
Independent Variables

The work presents a method of obtaining an aqueous raspberry extract as well as its physicochemical and analytical characteristics. The paper also contains a description of the method of preparation of nanosilver suspensions based on this extract. The raspberry extract served as a source of phenolic compounds which acted as both reducing and stabilizing agents. Suspensions of silver nanoparticles were obtained with the use of chemical reduction method. The silver ions concentration, pH value and temperature of samples incubation were independent variables. The next step of the research was to measure the antifungal activity of the received silver nanoparticles as well as to perform a mycological efficacy resistance analysis of the tested preparations in relation to different concentrations of nanostructured silver. Tests were conducted in compliance with the Eucast guidelines. The results of microbiological study of (the samples') biocidal effect against Cladosporium cladosporoides and Aspergillus niger are described. It was found that using nanosilver suspension at the concentration of 50 ppm inhibited the growth of Cladosporium cladosporoides and Aspergillus niger by 90% and 70%, respectively.

scholarly journals A close analysis of metal-enhanced fluorescence of tryptophan induced by silver nanoparticles: wavelength emission dependence

2013 ◽  
Vol 11 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Anderson Caires ◽  
Luciano Costa ◽  
Joelson Fernandes
Keyword(s):  
Silver Nanoparticles ◽  
Inner Filter Effect ◽  
Label Free ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Promising Alternative ◽  
Close Analysis ◽  
Label Free Detection ◽  
Nanoparticles Concentration ◽  
Wavelength Emission

AbstractIn the last few years, silver nanoparticles have been proposed as a promising alternative for the label-free detection of proteins via metal-enhanced fluorescence. Generally, the aromatic amino acid tryptophan is most frequently used in this type of studies, because the intrinsic fluorescence of proteins is usually dominated by tryptophan emissions. In the present study, we evaluated the fluorescence behavior of tryptophan in the presence of a silver colloid with nanoparticles of 100 nm in diameter. The results showed that a nanoparticles concentration of 32 mg L−1 induced maximum fluorescence enhancement. However, the metal-enhanced fluorescence was dependent on the emission wavelength of tryptophan, and this phenomenon was closely related to the metal surface reabsorption process (inner filter effect), suggesting that the plasmon resonance reabsorption effect should be taken into account in analyses involving protein studies by metal-enhanced fluorescence.

Ag@SiO2-entrapped hydrogel microarray: a new platform for a metal-enhanced fluorescence-based protein assay

The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3375-3383 ◽  
Author(s):  
Eunji Jang ◽  
Minsu Kim ◽  
Won-Gun Koh
Keyword(s):  
Silver Nanoparticles ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Protein Assay ◽  
Poly Ethylene

We developed a novel silver-based metal-enhanced fluorescence (MEF) biosensing platform that consisted of poly(ethylene glycol)(PEG) hydrogel microstructures entrapping silica-coated silver nanoparticles (Ag@SiO2).

Study on the interaction of berberine with nucleic acids in the presence of silver nanoparticles, and the fluorometric determination of nucleic acids

RSC Advances ◽  
2016 ◽  
Vol 6 (35) ◽  
pp. 29612-29618 ◽  
Author(s):  
Yanyan Zhao ◽  
Haiping Zhou ◽  
Jin Shen ◽  
Minqin Wang ◽  
Xia Wu
Keyword(s):  
Silver Nanoparticles ◽  
Nucleic Acids ◽  
Enhanced Fluorescence ◽  
Fluorometric Determination ◽  
Metal Enhanced Fluorescence ◽  
Intercalation Binding ◽  
Sensitive Method

The partial intercalation binding between BER and ctDNA, and the anti-photobleaching ability of BER were both strengthened by AgNPs. A metal enhanced fluorescence-based sensitive method for the determination of nucleic acids was obtained.

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