Highly sensitive metal-enhanced fluorescence biosensor prepared on electrospun fibers decorated with silica-coated silver nanoparticles

2019 ◽  
Vol 284 ◽  
pp. 140-147 ◽  
Author(s):  
Byung Ju Yun ◽  
Ji Eon Kwon ◽  
Kangwon Lee ◽  
Won-Gun Koh
2015 ◽  
Vol 44 (2) ◽  
pp. 135-137 ◽  
Author(s):  
Ryusuke Yoshida ◽  
Takuya Matsumura ◽  
Tsukasa Nakahodo ◽  
Hisashi Fujihara

2012 ◽  
Vol 602-604 ◽  
pp. 187-191 ◽  
Author(s):  
Chun Yang ◽  
Feng Yan Ge ◽  
Jin Cai Li ◽  
Zai Sheng Cai ◽  
Fang Fang Qin

Silver nanoparticles were prepared by sodium borohydride reduction method for analyzing metal-enhanced fluorescence property. Some variables including the dosage of reagent, reacting temperature and pH value had been investigated. Subsequently, a comparison of metal-enhanced fluorescence efficiency was made between two kinds of fluorescent dyes, namely fluorescein and 6-carboxyfluorescein at different silver concentrations. The experimental results show that the fluorescence of both dyes are remarkably enhanced. It is interesting to note that the increase of emission intensity is stronger than that of their corresponding excitation ones. Furthermore, a 5.038-fold increase in fluorescence for 6-carboxyfluorescein while a 2.506-fold increase for fluorescein are observed. This may attribute to the interaction between dyes and silver nanoparticels.


2013 ◽  
Vol 11 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Anderson Caires ◽  
Luciano Costa ◽  
Joelson Fernandes

AbstractIn the last few years, silver nanoparticles have been proposed as a promising alternative for the label-free detection of proteins via metal-enhanced fluorescence. Generally, the aromatic amino acid tryptophan is most frequently used in this type of studies, because the intrinsic fluorescence of proteins is usually dominated by tryptophan emissions. In the present study, we evaluated the fluorescence behavior of tryptophan in the presence of a silver colloid with nanoparticles of 100 nm in diameter. The results showed that a nanoparticles concentration of 32 mg L−1 induced maximum fluorescence enhancement. However, the metal-enhanced fluorescence was dependent on the emission wavelength of tryptophan, and this phenomenon was closely related to the metal surface reabsorption process (inner filter effect), suggesting that the plasmon resonance reabsorption effect should be taken into account in analyses involving protein studies by metal-enhanced fluorescence.


The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3375-3383 ◽  
Author(s):  
Eunji Jang ◽  
Minsu Kim ◽  
Won-Gun Koh

We developed a novel silver-based metal-enhanced fluorescence (MEF) biosensing platform that consisted of poly(ethylene glycol)(PEG) hydrogel microstructures entrapping silica-coated silver nanoparticles (Ag@SiO2).


RSC Advances ◽  
2016 ◽  
Vol 6 (35) ◽  
pp. 29612-29618 ◽  
Author(s):  
Yanyan Zhao ◽  
Haiping Zhou ◽  
Jin Shen ◽  
Minqin Wang ◽  
Xia Wu

The partial intercalation binding between BER and ctDNA, and the anti-photobleaching ability of BER were both strengthened by AgNPs. A metal enhanced fluorescence-based sensitive method for the determination of nucleic acids was obtained.


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