scholarly journals Spermiogenesis and ultrastructure of the spermatozoon of Wardula capitellata (Digenea, Mesometridae), an intestinal parasite of the sparid teleost Sarpa salpa in Senegal

2012 ◽  
Vol 57 (1) ◽  
Author(s):  
Abdoulaye Bakhoum ◽  
Papa Ndiaye ◽  
Aminata Sène ◽  
Cheikh Bâ ◽  
Jordi Miquel
Keyword(s):  
Plasma Membrane ◽  
Intestinal Parasite ◽  
Mature Spermatozoon ◽  
Cytoplasmic Process ◽  
Cortical Microtubules ◽  
Differentiation Zone ◽  
Sarpa Salpa ◽  
First Time ◽  
External Ornamentation ◽  
Free Flagellum

AbstractThe spermiogenesis process in Wardula capitellata begins with the formation of a differentiation zone containing two centrioles associated with striated rootlets and an intercentriolar body. Each centriole develops into a free flagellum orthogonal to a median cytoplasmic process. Later these flagella rotate and become parallel to the median cytoplasmic process, which already exhibits two electron-dense areas and spinelike bodies before its proximodistal fusion with the flagella. The final stage of the spermiogenesis is characterized by the constriction of the ring of arched membranes, giving rise to the young spermatozoon, which detaches from the residual cytoplasm. The mature spermatozoon of W. capitellata presents most of the classical characters reported in digenean spermatozoa such as two axonemes of different lengths of the 9 + “1” trepaxonematan pattern, nucleus, mitochondrion, two bundles of parallel cortical microtubules and granules of glycogen. However, some peculiarities such as two lateral expansions accompanied by external ornamentation of the plasma membrane and spinelike bodies characterize the mature sperm. Moreover, a new spermatological character is described for the first time, the so-called cytoplasmic ornamented buttons.

Spermatological characteristics of Elstia stossichianum (Digenea, Mesometridae) from the intestine of the cow bream (Sarpa salpa) off Dakar, Senegal

2012 ◽  
Vol 87 (4) ◽  
pp. 422-431 ◽  
Author(s):  
A.J.S. Bakhoum ◽  
P.I. Ndiaye ◽  
C.T. Bâ ◽  
J. Miquel
Keyword(s):  
Mature Spermatozoon ◽  
Cytoplasmic Process ◽  
Cortical Microtubules ◽  
Lateral Expansion ◽  
Differentiation Zone ◽  
Sarpa Salpa ◽  
Ultrastructural Characteristics ◽  
First Time ◽  
External Ornamentation ◽  
Free Flagellum

AbstractThe current study was designed to increase the spermatological ultrastructural database on Digenea, thus contributing to the future establishment of phylogenetic relationships within this group based on ultrastructural characteristics of both spermiogenesis and spermatozoa. Spermiogenesis in Elstia stossichianum begins with the formation of a differentiation zone containing two centrioles, two striated rootlets, a nucleus, several mitochondria and an intercentriolar body constituted by seven electron-dense layers. Each centriole develops into a free flagellum growing orthogonally to the median cytoplasmic process. Proximo-distal fusion of the flagella with the median cytoplasmic process occurs after flagellar rotation. Both nucleus and mitochondria migrate before the complete proximo-distal fusion of both flagella with the median cytoplasmic process. Finally, the constriction of the ring of arched membranes gives rise to the young spermatozoon. The mature spermatozoon of E. stossichianum exhibits two axonemes of different length of the 9+‘1’ trepaxonematan pattern, a nucleus, a mitochondrion, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, a lateral expansion, spine-like bodies, cytoplasmic ornamented buttons and granules of glycogen. The formation of cytoplasmic ornamented buttons during the final stages of spermiogenesis is reported here for the first time.

scholarly journals Ultrastructure of mature spermatozoa of three Bucephalidae (Prosorhynchus longisaccatus, Rhipidocotyle khalili and Bucephalus margaritae) and phylogenetic implications

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 65 ◽  
Author(s):  
Papa Ibnou Ndiaye ◽  
Bernard Marchand ◽  
Cheikh Tidiane Bâ ◽  
Jean-Lou Justine ◽  
Rodney A. Bray ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Anterior Extremity ◽  
Posterior Extremity ◽  
Anterior Part ◽  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
Lateral Expansion ◽  
Phylogenetic Implications ◽  
External Ornamentation ◽  
Mature Spermatozoa

We describe here the mature spermatozoa of three species of bucephalids, namely Bucephalus margaritae, Rhipidocotyle khalili and Prosorhynchus longisaccatus. This study provides the first ultrastructural data on the genera Bucephalus and Rhipidocotyle and enabled us to confirm the model of the mature spermatozoon in the Bucephalinae. The spermatozoon exhibits two axonemes with the 9 + “1” pattern of the Trepaxonemata, one of which is very short, lateral expansion, external ornamentation of the plasma membrane located in the anterior extremity of the spermatozoon and associated with cortical microtubules, spine-like bodies, a mitochondrion, and a nucleus. The maximum number of cortical microtubules is located in the anterior part of the spermatozoon. However, more studies are needed to elucidate if spine-like bodies are present in all the Bucephalinae or not. In the Prosorhynchinae, the mature spermatozoon exhibits a similar ultrastructural pattern. Some differences are observed, particularly the axoneme lengths and the arrangement of the spine-like bodies. The posterior extremity of the spermatozoon in the Bucephalinae exhibits only the nucleus, but prosorhynchines have microtubules.

Ultrastructure of spermiogenesis and mature spermatozoon of Skrjabinoporus merops (Cyclophyllidea, Metadilepididae)

2006 ◽  
Vol 51 (3) ◽  
Author(s):  
Aneta Yoneva ◽  
Katia Georgieva ◽  
Yana Mizinska ◽  
Boyko Georgiev ◽  
Stoyanka Stoitsova
Keyword(s):  
Proximal Part ◽  
Mature Spermatozoon ◽  
Differentiation Zone ◽  
Region I ◽  
The Family ◽  
Cytoplasmic Protrusion ◽  
Flagellar Rotation ◽  
First Time ◽  
Region Ii ◽  
Free Flagellum

AbstractThe ultrastructure of the mature spermatozoon and the spermiogenesis of a cestode belonging to the family Metadilepididae is described for the first time. The mature spermatozoon of Skrjabinoporus merops is characterized by twisted peripheral microtubules, the presence of a single crested body, periaxonemal sheath and electron-dense rods, and the absence of intracytoplasmic walls and inclusions (glycogen or proteinaceous granules); no peripheral microtubules where nucleus contacts the external plasma membrane. Four morphologically distinct regions of the mature spermatozoon are differentiated. The proximal part (Region I) contains a single crested body, periaxonemal sheath is absent in some (proximal) sections and is present in others situated closer to the nucleus. The central Region II is nucleated, and is followed by Region III that contains a periaxonemal sheath. The distal pole, Region IV, is characterized by disintegration of the axoneme. Spermiogenesis follows the type III pattern (Bâ and Marchand 1995) although in S. merops a slight flagellar rotation is observed. The differentiation zone is characterized by the absence of striated roots and intercentriolar body; two centrioles are present, one of which gives rise to a free flagellum. The latter rotates and undergoes proximodistal fusion with the cytoplasmic protrusion of the differentiation zone. Spermiological characters of S. merops are similar to those of the families Taeniidae and Catenotaeniidae. The mature spermatozoon differs from those of the Dilepididae (where the metadilepidid species have previously been classified) by the lack of glycogen.

Ultrastructure of spermiogenesis and mature spermatozoon of Angularella beema (Clerc, 1906) (Cestoda, Cyclophyllidea, Dilepididae)

2006 ◽  
Vol 51 (4) ◽  
Author(s):  
Aneta Yoneva ◽  
Jordi Miquel ◽  
Zdzisław Świderski ◽  
Katia Georgieva ◽  
Yana Mizinska ◽  
...  
Keyword(s):  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
Cestode Species ◽  
Family Presence ◽  
Differentiation Zone ◽  
Type Iv ◽  
The Family ◽  
Cytoplasmic Protrusion ◽  
Ultrastructural Characteristics ◽  
First Time

AbstractThe ultrastructure of the spermiogenesis of a dilepidid cestode species is described for the first time. The spermiogenesis of Angularella beema is characterised by absence of both flagellar rotation and proximodistal fusion. The differentiation zone is surrounded by cortical microtubules and is delimited by a ring of arching membranes. It contains two centrioles, one of which develops the axoneme that grows directly into the elongating cytoplasmic protrusion. This pattern of spermiogenesis was described as the Type IV spermiogenesis of cestodes. Among cestodes, similar pattern of spermiogenesis is known in the family Hymenolepididae and in some representatives of the family Anoplocephalidae. The mature spermatozoon of A. beema consists of five regions differing in their ultrastructural characteristics. It is characterised by the presence of cortical microtubules (spirally arranged at angle of 30–40° to the spermatozoon axis) and a single crested body. There is a periaxonemal sheath in certain parts of the spermatozoon as well as glycogen-like granules between the periaxonemal sheath and the cortical microtubules. The comparisons of the mature spermatozoon of A. beema with those of other two dilepidid species (Dilepis undula and Molluscotaenia crassiscolex) demonstrate some variation within the family: presence of periaxonemal sheath in A. beema and D. undula and its absence in M. crassiscolex; presence of electron-dense rods in D. undula and their absence in A. beema.

scholarly journals Spermatozoon ultrastructure in two monorchiid digeneans

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2488 ◽  
Author(s):  
Yann Quilichini ◽  
Abdoulaye J.S. Bakhoum ◽  
Jean-Lou Justine ◽  
Rodney A. Bray ◽  
Cheikh T. Bâ ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Male Gamete ◽  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
General Morphology ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
First Time ◽  
External Ornamentation ◽  
Phylogenetical Relationship

Spermatological characteristics of species from two monorchiid genera,OpisthomonorchisandParamonorcheides, have been investigated, for the first time, by means of transmission electron microscopy. The ultrastructural study reveals that the mature spermatozoon ofOpisthomonorchis dinemaandParamonorcheides selarisshare several characters such as the presence of two axonemes of different lengths showing the 9+“1” pattern of the Trepaxonemata, a nucleus, two mitochondria, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, spine-like bodies, granules of glycogen and similar morphologies of the anterior and posterior extremities. The slight differences between the male gamete ofO. dinemaandP. selarisare the length of the first axoneme and the position of the second mitochondrion. This study also elucidates the general morphology of the spermatozoon in all monorchiid species described so far, which corresponds to a unique spermatozoon type. Other interesting finds concern the spermatological similarities between monorchiid spermatozoa and the mature spermatozoon reported in the apocreadiidNeoapocreadium chabaudi. These similarities allow us to suggest a close phylogenetical relationship between the Monorchiidae and the Apocreadiidae, although more studies are needed, especially in the unexplored taxa.

scholarly journals A comparative study of the ultrastructural characteristics of the mature spermatozoa of two fellodistomids Tergestia clonacantha and T. laticollis and contribution to the phylogenetic knowledge of the Gymnophalloidea

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 67
Author(s):  
Papa Ibnou Ndiaye ◽  
Bernard Marchand ◽  
Cheikh Tidiane Bâ ◽  
Jean-Lou Justine ◽  
Rodney Alan Bray ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
New Caledonia ◽  
General Pattern ◽  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
Transmission Electron ◽  
Ultrastructural Model ◽  
Ultrastructural Characteristics ◽  
External Ornamentation ◽  
Mature Spermatozoa

The ultrastructure of the mature spermatozoa of Tergestia clonacantha and T. laticollis collected from the digestive tracts of fishes from New Caledonia is described using transmission electron microscopy and compared to that of related species. The spermatozoa of the two species exhibit the general pattern described in most digeneans, namely two axonemes with the 9 + “1” pattern of the Trepaxonemata, nucleus, mitochondrion, cortical microtubules, an external ornamentation of the plasma membrane, spine-like bodies and granules of glycogen. The spermatozoa of T. clonacantha and T. laticollis show the same ultrastructural model with some specificities in each case, particularly in the disposition of the structures in the posterior extremities of the spermatozoon. This study confirms that ultrastructural characters of the mature spermatozoon are useful tools for the phylogenetic analysis of the Digenea.

Ultrastructure of spermiogenesis in the caryophyllidean cestode Wenyonia virilis Woodland, 1923, with re-assessment of flagellar rotation in Glaridacris catostomi Cooper, 1920

2008 ◽  
Vol 53 (1) ◽  
Author(s):  
Jordi Miquel ◽  
Zdzisław Świderski ◽  
John Mackiewicz ◽  
Mohammed Ibraheem
Keyword(s):  
Unique Type ◽  
Differentiation Zone ◽  
Cytoplasmic Extension ◽  
Transmission Electron ◽  
Original Report ◽  
Flagellar Rotation ◽  
Means Of Transmission ◽  
First Time ◽  
Apical Area ◽  
Free Flagellum

AbstractThe ultrastructure of spermiogenesis in Wenyonia virilis Woodland, 1923, a caryophyllaeid cestode from the silurid Nile fish Synodontis schall (Bloch et Schneider, 1801), is described by means of transmission electron microscopy (TEM) for the first time. Spermiogenesis follows the characteristic caryophyllidean type and is initiated by the formation of a differentiation zone. This area, delimited at its base by a ring of arching membranes and bordered by cortical microtubules, contains two centrioles associated with typical striated rootlets with a reduced intercentriolar body between them. The apical area of the differentiation zone exhibits electron-dense material that is present only during the early stages of spermiogenesis. Only one of the centrioles develops into a free flagellum that grows at an angle of >90° in relation to the cytoplasmic extension. Spermiogenesis is also characterized by a flagellar rotation and a proximodistal fusion of the flagellum with the cytoplasmic extension. The most interesting features observed in W virilis are the presence of a reduced, very narrow intercentriolar body and the unique type of flagellar rotation >90°. Results are compared with those described in two caryophyllideans, Glaridacris catostomi Cooper, 1920 and Khawia armeniaca (Cholodkovski, 1915). Contrary to the original report of Świderski and Mackiewicz (2002), that flagellar rotation has never been observed in spermiogenesis of G. catostomi, re-assessment of their description and illustrations leads us to conclude that flagellar rotation must logically occur in that species. The value of various morphological features of sperm in phylogenetic inference is discussed.

scholarly journals Ultrastructure and cytochemistry of the mature spermatozoon of Khawia armeniaca (Cholodkovsky, 1915) (Caryophyllidea: Lytocestidae), a parasite of Capoeta capoeta sevangi (De Filippi, 1865) (Teleostei, Cyprinidae)

2020 ◽  
Vol 57 (4) ◽  
pp. 353-360
Author(s):  
M. Matoušková ◽  
M. Bruňanská ◽  
J. Nebesářová ◽  
L. G. Poddubnaya
Keyword(s):  
Anterior Part ◽  
Male Gamete ◽  
Longitudinal Axis ◽  
Mature Spermatozoon ◽  
Ultrastructural Organization ◽  
Cortical Microtubules ◽  
Transmission Electron ◽  
Cytochemical Technique ◽  
First Time ◽  
Region Ii

SummaryThe mature spermatozoon of Khawia armeniaca, a monozoic caryophyllidean parasite of templar fish Capoeta capoeta sevangi (De Filippi, 1865) from the Lake Sevan, Armenia, has been studied using transmission electron microscopy and cytochemical technique of Thiéry (1967) for the first time. The mature spermatozoon of K. armeniaca consists of a single axoneme with the 9+‘1’ trepaxonematan structure, cortical microtubules and nucleus which are situated parallel to the longitudinal axis of the spermatozoon, and a moderately electrondense cytoplasm with glycogen particles. The cortical microtubules are arranged in one continuous semicircle beneath the plasma membrane in Region II and anterior part of Region III of the mature spermatozoon. The two opposite rows of cortical microtubules are observed in the remaining nuclear and at the beginning of the postnuclear part (Regions III, IV) of the male gamete The number of cortical microtubules is remarkably variable in the spermatozoa of various Khawia species. K. armeniaca exhibits the highest number of cortical microtubules in comparison with K. sinensis and K. rossittensis. Glycogen was detected in the cytoplasm of prenuclear (II), nuclear (III) and postnuclear (IV) regions with different ultrastructural organization of the mature spermatozoon of K. armeniaca. Variations of sperm ultrastructural characters within caryophyllideans and other cestodes are discussed.

Ultrastructural study of spermiogenesis and the spermatozoon in Catenotaenia pusilla, an intestinal parasite of Mus musculus

2000 ◽  
Vol 74 (1) ◽  
pp. 73-81 ◽  
Author(s):  
C. Hidalgo ◽  
J. Miquel ◽  
J. Torres ◽  
B. Marchand
Keyword(s):  
Cross Section ◽  
Anterior Extremity ◽  
Intestinal Parasite ◽  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
Spiral Pattern ◽  
Cytoplasmic Extension ◽  
Apical Cone ◽  
Flagellar Rotation ◽  
Unequal Length

AbstractThe ultrastructure of spermiogenesis and the mature spermatozoon in Catenotaenia pusilla (Cestoda: Catenotaeniidae) is described. Spermiogenesis is characterized by the presence of a single axoneme which grows on the outside of a cytoplasmic extension at an angle of 45°. Flagellar rotation and proximodistal fusion are produced in this process. The centrioles lack striated roots and an intercentriolar body. In the mature spermatozoon four different regions are described. The anterior extremity is capped by an apical cone and presents two helical crest-like bodies of unequal length. The axoneme, of the 9 + ‘1’ pattern of the Trepaxonemata, presents a periaxonemal sheath. The cortical microtubules form a spiral pattern at an angle of about 40° to the hypothetical spermatozoon axis. The nucleus is kidney- to horseshoe-shaped in cross section. Granules and proteinaceus walls are not observed in the spermatozoon of C. pusilla.

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