scholarly journals SEM evidence for existence of an apical disc on the scolex of Clestobothrium crassiceps (Rudolphi, 1819): comparative results of various fixation techniques

2012 ◽  
Vol 57 (3) ◽  
Author(s):  
Jordi Miquel ◽  
Zdzisław Świderski ◽  
Adji Marigo ◽  
Eulàlia Delgado ◽  
Jordi Torres
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Comparative Analysis ◽  
First Report ◽  
Longitudinal Groove ◽  
Apical Disc ◽  
Fixation Techniques ◽  
Comparative Results ◽  
Scanning Electron ◽  
Clestobothrium Crassiceps

AbstractThe scolex of the bothriocephalidean cestode Clestobothrium crassiceps was studied by means of scanning electron microscopy (SEM). The comparative results of various fixation procedures and techniques are presented. The scolex of C. crassiceps is oval to globular and exhibits two deep bothria which appear in the form of two lobes separated by a longitudinal groove. At the apex of the scolex, resembling a beret, an apical disc is present (oval, flattened and with a sinuous edge). Our results are compared with those previously reported in other species of Clestobothrium. This study represents the first report which highlights the presence of an apical disc in the scolex of C. crassiceps. It describes the effects of different procedures applied to our material during preparation and a comparative analysis results obtained using these various methods.

A Comparative Analysis of New Ti-Nb-Zr-Ta Orthopedic Alloys

2013 ◽  
Vol 837 ◽  
pp. 259-264 ◽  
Author(s):  
Andreea Carmen Bărbînţă ◽  
Romeo Chelariu ◽  
Marcelin Benchea ◽  
Carmen Iulia Crimu ◽  
Sorin Iacob Strugaru ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Comparative Analysis ◽  
Bone Resorption ◽  
Modulus Of Elasticity ◽  
Human Tissues ◽  
X Ray Diffraction ◽  
X Ray ◽  
New Generation ◽  
Scanning Electron

Ti-Nb-Zr-Ta alloys represent a new generation of biomaterials with possible applications in the orthopedic field, being developed in order to eliminate the negative aspects of the current orthopedic biomaterials, which consist mainly in a low biocompatibility with human tissues and high values of modulus of elasticity compared to the human bone. This paper presents a comparative study of new titanium alloys, corresponding to the Ti-Nb-Zr-Ta system: Ti-21Nb-6Zr-15Ta and Ti-25Nb-10Zr-8Ta, which were analyzed by scanning electron microscopy, X-ray diffraction and microindentation. The both alloys are classified as near-β alloys. The addition of alloying elements such as Ta, Nb and Zr represents a good solution for lowering modulus of elasticity, which is an important factor for reducing bone resorption and therefore for preventing implant failure.

scholarly journals Evaluation of two fixation techniques for direct observation of biofilm formation of Bacillus subtilis in situ, on Congo red agar, using scanning electron microscopy

2020 ◽  
Vol 13 (6) ◽  
pp. 1133-1137
Author(s):  
Nadia Mahmoud Tawfiq Jebril
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Direct Observation ◽  
Congo Red ◽  
Fixation Method ◽  
Fixation Technique ◽  
Fixation Techniques ◽  
Biofilm Development ◽  
Scanning Electron

Background and Aim: Direct observation, scanning electron microscopy (SEM) is a common method used for the observations of biofilms. N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide) (EDC) fixation method has proven to be a valuable fixation method in the observation of these biofilms. Still, it entails a method of biofilm fixation that can damage slim structures, leading to the impossible observation of biofilm development. In contrast, alcian blue and lysine (ABL) fixation technique appears more glycocalyx of biofilm, fully preserved samples, which may provide much insight into the development of B. subtilis biofilms. Materials and Methods: Here, the evaluation of the fixation of ABL technique for the study of B. subtilis biofilms was carried out in situ, on Congo red agar. In doing so, the comparison to commonly use conventional EDC technique for sample fixation, and observation was carried out. Observations were based on SEM over 30 samples. Results: Overall, ABL technique provided excellent observation of biofilms formed in situ, on Congo red agar, and revealed slime structures, which have not been observed, much in standard EDC fixation or earlier in other studies of these biofilms in B. subtilis. Conclusion: This study reported the appropriate use of ABL in the fixation technique for the preservation of biofilm of B. subtilis.

scholarly journals Nickel-based catalysts: Dependence of properties on nickel loading and modification with palladium

2016 ◽  
Vol 70 (2) ◽  
pp. 137-142 ◽  
Author(s):  
Vesna Nikolic ◽  
Zeljko Kamberovic ◽  
Marija Korac ◽  
Zoran Andjic ◽  
Aleksandar Mihajlovic ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Comparative Analysis ◽  
Chemical Analysis ◽  
Energy Dispersive Spectroscopy ◽  
Hydrogen Reduction ◽  
Energy Dispersive ◽  
Complete Reduction ◽  
Scanning Electron

The aim of this study was comparative analysis of catalysts with 5 and 20 wt. % Ni loading, with and without Pd additive, and it included: determination of reduction degrees, investigation on microstructure by scanning electron microscopy and chemical analysis by energy-dispersive spectroscopy. Ni/Al2O3 and Ni-Pd/Al2O3 catalysts supported on ?-Al2O3-based foams were synthesized by aerosol assisted impregnation. Impregnation of the foams by ultrasonically aerosolized chlorides was followed by drying at 473 K and hydrogen reduction at 533 and 633 K. Lower Ni loading resulted in higher reduction degrees. Ni loading of 20 wt. % enabled relatively uniform coverage of the foam with a metallic coating. With activity modifier (Pd), nearly complete reduction was achieved at both temperatures. Reduction degrees reached 99.4 and 98.2 wt. % at 533 K for 5 and 20 wt. % of Ni, respectively.

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