In situ bioconversion of compactin to pravastatin by Actinomadura species in fermentation broth of Penicillium citrinum

AbstractThe biocatalytic production of pravastatin from compactin by hydroxylation has found many applications in health care and pharmaceuticals. Actinomadura macra, Actinomadura madurae, and Actinomadura livida can efficiently bioconvert compactin to pravastatin. The fermentation broth (Penicillium citrinum fermented media) harvested on the eighth day contained 388.90 mg L−1 of compactin and an undetectable level of mycotoxin (citrinin). Bioconversion by A. macra was highest (87 %) in the yeast extract-amended medium. The anti-actinomadura effects of citrinin reduce the bioconversion capacity of Actinomadura. The in situ hydroxylation of compactin produced by P. citrinum represents a preferable alternative for the use of purified compactin, as a way to reduce cost and time processing.

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Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica

Biotechnology for Biofuels ◽

10.1186/s13068-019-1576-7 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 3

Author(s):

Pratik Prashant Pawar ◽

Annamma Anil Odaneth ◽

Rajeshkumar Natwarlal Vadgama ◽

Arvind Mallinath Lali

Keyword(s):

Yarrowia Lipolytica ◽

Oil Recovery ◽

Oleaginous Yeast ◽

Fermentation Broth ◽

Continuous Production ◽

Oil Production ◽

Biofuel Production ◽

Oil Yield ◽

Microbial Oil

Abstract Background Recent trends in bioprocessing have underlined the significance of lignocellulosic biomass conversions for biofuel production. These conversions demand at least 90% energy upgradation of cellulosic sugars to generate renewable drop-in biofuel precursors (Heff/C ~ 2). Chemical methods fail to achieve this without substantial loss of carbon; whereas, oleaginous biological systems propose a greener upgradation route by producing oil from sugars with 30% theoretical yields. However, these oleaginous systems cannot compete with the commercial volumes of vegetable oils in terms of overall oil yields and productivities. One of the significant challenges in the commercial exploitation of these microbial oils lies in the inefficient recovery of the produced oil. This issue has been addressed using highly selective oil capturing agents (OCA), which allow a concomitant microbial oil production and in situ oil recovery process. Results Adsorbent-based oil capturing agents were employed for simultaneous in situ oil recovery in the fermentative production broths. Yarrowia lipolytica, a model oleaginous yeast, was milked incessantly for oil production over 380 h in a media comprising of glucose as a sole carbon and nutrient source. This was achieved by continuous online capture of extracellular oil from the aqueous media and also the cell surface, by fluidizing the fermentation broth over an adsorbent bed of oil capturing agents (OCA). A consistent oil yield of 0.33 g per g of glucose consumed, corresponding to theoretical oil yield over glucose, was achieved using this approach. While the incorporation of the OCA increased the oil content up to 89% with complete substrate consumptions, it also caused an overall process integration. Conclusion The nondisruptive oil capture mediated by an OCA helped in accomplishing a trade-off between microbial oil production and its recovery. This strategy helped in realizing theoretically efficient sugar-to-oil bioconversions in a continuous production process. The process, therefore, endorses a sustainable production of molecular drop-in equivalents through oleaginous yeasts, representing as an absolute microbial oil factory.

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Enhanced biocatalytic production of l-cysteine by Pseudomonas sp. B-3 with in situ product removal using ion-exchange resin

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-014-1281-7 ◽

2014 ◽

Vol 38 (3) ◽

pp. 421-428 ◽

Cited By ~ 4

Author(s):

Pu Wang ◽

Jun-Yao He ◽

Jiang-Feng Yin

Keyword(s):

Ion Exchange ◽

Exchange Resin ◽

Ion Exchange Resin ◽

Pseudomonas Sp ◽

In Situ Product Removal ◽

Product Removal ◽

Biocatalytic Production

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In-Situ recovery of ethanol from fermentation broth by hydrophobic adsorbents

Acta Biotechnologica ◽

10.1002/abio.370110411 ◽

1991 ◽

Vol 11 (4) ◽

pp. 353-358 ◽

Cited By ~ 9

Author(s):

W. D. Einicke ◽

B. Gläser ◽

R. Schöoullner

Keyword(s):

Fermentation Broth ◽

Hydrophobic Adsorbents ◽

In Situ Recovery

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Surgical Pathology–Based Outcomes Assessment of Breast Cancer Early Diagnosis

Archives of Pathology & Laboratory Medicine ◽

10.5858/2001-125-0325-spboao ◽

2001 ◽

Vol 125 (3) ◽

pp. 325-331

Author(s):

Raouf E. Nakhleh ◽

Richard J. Zarbo

Keyword(s):

Breast Cancer ◽

Health Care ◽

The United States ◽

Surgical Pathology ◽

Detection Methods ◽

Mortality Data ◽

Breast Cancers ◽

Improvement Program ◽

Clinical Method

Abstract Objective.—To develop breast cancer outcomes data relating pathologic tumor variables at diagnosis with clinical method of detection. Design.—Anatomic pathologists assessed 30 consecutive breast cancers at each institution, resulting in an aggregate database of 4232 breast cancers. Setting.—Hospital-based laboratories from the United States (98%), Canada, Australia, and Belgium. Participants.—One hundred ninety-nine laboratories in the 1999 College of American Pathologists Q-Probes voluntary quality improvement program. Main Outcome Measures.—Pathologic variables indicative of favorable outcomes included percentage of carcinomas detected at the in situ stage, tumors ≤1 cm in diameter, and invasive cancers with lymph nodes negative for metastases. Results.—All outcomes measures, including percent in situ carcinomas (26.9% vs 13.8%), tumor size ≤1 cm (57.8% vs 36.5%), and lymph node–negative status (77.8% vs 64%), were more favorable when tumors were detected by screening mammography (P < .001) compared to all other detection methods. Conclusions.—This study demonstrates an opportunity for pathologists to develop outcomes information of interest to health care organizations, providers, patients, and payers by integrating routine oncologic surgical pathology and clinical breast cancer detection data. Such readily obtained interim outcomes data trended and benchmarked over time can demonstrate the relative clinical efficacy of preventive breast care provided by health care systems long before mortality data are available.

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Improved Biobutanol Production in 2-L Simultaneous Saccharification and Fermentation with Delayed Yeast Extract Feeding and in-situ Recovery

Scientific Reports ◽

10.1038/s41598-019-43718-1 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 6

Author(s):

Muhammad Siddiq Mohamed Salleh ◽

Mohamad Faizal Ibrahim ◽

Ahmad Muhaimin Roslan ◽

Suraini Abd-Aziz

Keyword(s):

Yeast Extract ◽

Simultaneous Saccharification And Fermentation ◽

Simultaneous Saccharification ◽

In Situ Recovery

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Ovarian steroids regulate 24p3 expression in mouse uterus during the natural estrous cycle and the preimplantation period

Journal of Endocrinology ◽

10.1677/joe.0.1620011 ◽

1999 ◽

Vol 162 (1) ◽

pp. 11-19 ◽

Cited By ~ 38

Author(s):

HL Huang ◽

ST Chu ◽

YH Chen

Keyword(s):

Gene Expression ◽

Estrous Cycle ◽

Immunohistochemical Localization ◽

The Other ◽

Undetectable Level ◽

Mouse Uterus ◽

Ovarian Steroids ◽

Vaginal Plug ◽

High Level

We examined 24p3 expression in the mouse uterus at various stages of the natural estrous cycle and during the preimplantation period. The level of 24p3 mRNA appeared intensively in proestrus and estrus, then declined sharply from metestrus to diestrus. Consistent with this observation, 24p3 protein was abundant in proestrus, decreased from estrus to metestrus and declined to a very low level in diestrus. The uterine 24p3 expression closely overlapped with the estradiol (E2) surge in proestrus and estrus but it was suppressed when progesterone (P4) rose to a high level during the reproductive cycle. Neither the protein nor its message was detected in the uteri of immature mice or ovariectomized adult animals. While an injection of P4 to these animals was unable to initiate uterine 24p3 expression, administration of estrogenic steroids to these animals markedly stimulated the gene expression. Treatment of these animals with E2 together with P4, on the other hand, did not stimulate the gene expression. In pregnant animals (day 1 (D1)=day of vaginal plug), 24p3 mRNA remained at a high level on D1 and D2 but dropped to an almost undetectable level on D3 and D4. This was accompanied by a decrease in 24p3 protein from D1 to D2 and a decline in the protein to undetectable levels from D3 to D4. The staining patterns of both the immunohistochemical localization of 24p3 protein and in situ hybridization for the detection of 24p3 mRNA in the uterine sections showed that 24p3 expression took place mainly in the luminal and glandular epithelial cells of the endometrium. This together with our previous observation that 24p3 protein is found in uterine luminal fluid indicates that the protein is secreted primarily from these cells to their respective luminal surfaces during proestrus and estrus.

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A simple method for the production of low molecular weight hyaluronan by in situ degradation in fermentation broth

e-Polymers ◽

10.1515/epoly-2019-0050 ◽

2019 ◽

Vol 19 (1) ◽

pp. 477-481

Author(s):

Jianfeng Mei ◽

Zhihong Dong ◽

Yu Yi ◽

Yanlu Zhang ◽

Guoqing Ying

Keyword(s):

Molecular Weight ◽

Enzymatic Degradation ◽

Fermentation Broth ◽

Low Molecular Weight ◽

Streptococcus Zooepidemicus ◽

Medium Ph ◽

Simple Method ◽

Suitable Temperature ◽

In Situ Degradation

AbstractFermentation of hyaluronan (HA) by Streptococcus zooepidemicus was carried out in a 10-L fermentor. When the medium pH was controlled at 7.0 and the temperature was maintained at 38°C for 12 h followed by 35°C for 8 h, the yield of HA was 4.83 g/L with a molecular weight of 1,890 kDa. After the cells were removed by centrifugation from the fermentation broth, HA was slowly degraded to low molecular weight HA by hyaluronidase at a suitable temperature without a decrease in HA concentration. If the time and temperature for enzymatic degradation were controlled, the desired low molecular weight HA could be obtained by in situ degradation in the fermentation broth. The method does not require the addition of exogenous hyaluronidase, and is a simple way to produce low molecular weight HA.

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In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sludges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I

Applied and Environmental Microbiology ◽

10.1128/aem.67.12.5740-5749.2001 ◽

2001 ◽

Vol 67 (12) ◽

pp. 5740-5749 ◽

Cited By ~ 87

Author(s):

Yuji Sekiguchi ◽

Hiroki Takahashi ◽

Yoichi Kamagata ◽

Akiyoshi Ohashi ◽

Hideki Harada

Keyword(s):

16S Rdna ◽

Yeast Extract ◽

Sea Urchins ◽

Granular Sludge ◽

Limited Range ◽

Strictly Anaerobic ◽

Sulfur Bacteria ◽

Filamentous Microorganisms ◽

Methanothermobacter Thermautotrophicus

ABSTRACT We previously showed that very thin filamentous bacteria affiliated with the division green non-sulfur bacteria were abundant in the outermost layer of thermophilic methanogenic sludge granules fed with sucrose and several low-molecular-weight fatty acids (Y. Sekiguchi, Y. Kamagata, K. Nakamura, A. Ohashi, H. Harada, Appl. Environ. Microbiol. 65:1280–1288, 1999). Further 16S ribosomal DNA (rDNA) cloning-based analysis revealed that the microbes were classified within a unique clade, green non-sulfur bacteria (GNSB) subdivision I, which contains a number of 16S rDNA clone sequences from various environmental samples but no cultured representatives. To investigate their function in the community and physiological traits, we attempted to isolate the yet-to-be-cultured microbes from the original granular sludge. The first attempt at isolation from the granules was, however, not successful. In the other thermophilic reactor that had been treating fried soybean curd-manufacturing wastewater, we found filamentous microorganisms to outgrow, resulting in the formation of projection-like structures on the surface of granules, making the granules look like sea urchins. 16S rDNA-cloning analysis combined with fluorescent in situ hybridization revealed that the projections were comprised of the uncultured filamentous cells affiliated with the GNSB subdivision I and Methanothermobacter-like cells and the very ends of the projections were comprised solely of the filamentous cells. By using the tip of the projection as the inoculum for primary enrichment, a thermophilic, strictly anaerobic, filamentous bacterium, designated strain UNI-1, was successfully isolated with a medium supplemented with sucrose and yeast extract. The strain was a very slow growing bacterium which is capable of utilizing only a limited range of carbohydrates in the presence of yeast extract and produced hydrogen from these substrates. The growth was found to be significantly stimulated when the strain was cocultured with a hydrogen-utilizing methanogen, Methanothermobacter thermautotrophicus, suggesting that the strain is a sugar-fermenting bacterium, the growth of which is dependent on hydrogen consumers in the granules.

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