Growth enhancement of black pepper (Piper nigrum) by a newly isolated Bacillus tequilensis NII-0943

Biologia ◽  
2011 ◽  
Vol 66 (5) ◽  
Author(s):  
Syed Dastager ◽  
C. Deepa ◽  
Ashok Pandey
Keyword(s):  
Sequence Similarity ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Bacillus Species ◽  
Rrna Gene ◽  
Growth Enhancement ◽  
Nitrogen And Phosphorus ◽  
Bacillus Tequilensis ◽  
Plant Growth Promoting ◽  
The Western Ghats

AbstractA Gram positive, rod-shaped potential strain was selected from the pool of bacterial isolates obtained from the Western Ghats forest (India) on the basis of zone of P-solubilization activity. Identification based on 16S rRNA gene sequence revealed that the strain is of Bacillus species, sharing highest sequence similarity to Bacillus tequilensis NRRL B-41771T (99.5%). Strain NII-0943 was able to produce good amount of indole acetic acid (IAA) and was positive for siderophore production. In addition to IAA and siderophore attributes, strain NII-0943 also possessed the characteristics like Ca3(PO4)2 solubilization and growth in nitrogen-free medium. Seed inoculation with the strain NII-0943 resulted in significantly higher root initiation in black pepper cuttings grown under pots. The contents of nitrogen and phosphorus in both soil and plant were also enhanced significantly in treatments inoculated with these bacterial inocula. Hence, based on this evidence it is proposed that strain NII-0943 could be deployed as a plant growth-promoting inoculant to attain the desired results of bacterization.

scholarly journals Screening of Potent Arsenic Resistant and Plant Growth Promoting Bacillus species from the Soil of Terai Region of Nepal

2019 ◽  
Vol 6 ◽  
pp. 1-9
Author(s):  
Pramod Poudel ◽  
Ashish Nepal ◽  
Rashmi Roka Magar ◽  
Pratibha Rauniyar ◽  
Lil Buda Magar
Keyword(s):  
Plant Growth ◽  
Sodium Arsenite ◽  
16S Rrna Gene Sequencing ◽  
Bacillus Species ◽  
Rrna Gene ◽  
Sodium Arsenate ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rrna Gene Sequencing

Objectives: To isolate arsenic resistant Bacillus spp. and to determine plant growth promoting activities.  Methods: Eighteen soil samples were collected from the agricultural soil of Terai region of Nepal. Selective isolation of Bacillus species was done by heating the soil at 80 ºC for 15 minutes before the isolation. Nutrient agar was used as an isolation medium. Screening of arsenic resistant Bacillus species was done using nutrient agar supplemented with 100 ppm sodium arsenate and sodium arsenite. For plant growth promoting activity; IAA production was detected taking 0.1% tryptophane and measuring absorbance at 540 nm, NH3 production was tested by Nessler’s reagent and phosphate solubilization activity was detected by growing colonies on Pikovskaya’s agar. Sugar assimilation test was performed to identify the isolates. Most potent arsenic resistant isolate was identified by 16S rRNA gene sequencing. Results: Among 54 randomly selected isolates, 42 were found to be Gram-positive rod-shaped, spore-forming while 12 isolates were Gram-negative bacteria. The isolates IN12a, M12a and BG34a showed growth on 100 ppm sodium arsenite containing NA. Only isolate M12a tolerated up to 1000 ppm and 15000 ppm of sodium arsenite and sodium arsenate respectively, while other isolates could not grow above 400 ppm sodium arsenite. The isolates IN12a and M12a were able to produce IAA and solubilize phosphate while BG34a could not. Both the isolates IN12a and M12a were able to utilize the sugars glucose, fructose, lactose, sucrose, galactose, mannose, mannitol, maltose and xylose.  Based on the 16S rRNA gene sequencing, isolate M12a was identified to be Bacillus flexus with highest similarity of 99.2%. Conclusion: Arsenic resistant and plant growth promoting Bacillus spp. was isolated from the agricultural soil of Terai region of Nepal

scholarly journals Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils

2010 ◽  
Vol 60 (10) ◽  
pp. 2451-2457 ◽  
Author(s):  
Munusamy Madhaiyan ◽  
Selvaraj Poonguzhali ◽  
Jung-Sook Lee ◽  
Murugaiyan Senthilkumar ◽  
Keun Chul Lee ◽  
...  
Keyword(s):  
Plant Growth ◽  
Type Strain ◽  
Sequence Similarity ◽  
Extracellular Polysaccharide ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Separate Species ◽  
Genotypic Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Two isolates from rhizosphere soil of cotton, designated Gh-67T and Gh-48T, which produced large amounts of extracellular polysaccharide and possessed plant-growth-promoting traits, were characterized phenotypically and genotypically. The strains were Gram-negative and cells were non-motile rods that grew optimally at 28 °C and grew between pH 4 and 7. 16S rRNA gene sequence analysis of strains Gh-67T and Gh-48T placed them in the genus Mucilaginibacter, with pairwise sequence similarity between them and type strains from related genera ranging from 93.9 to 98.2 %. The major fatty acids were iso-C15 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). The strains contained MK-7 as the major isoprenoid quinone. The DNA G+C contents of strains Gh-67T and Gh-48T were 46.7 and 44.2 mol%, respectively. The low DNA–DNA hybridization value (18 %) and a number of phenotypic differences between strains Gh-48T and Gh-67T indicated that they represent two separate species. Results of phenotypic, phylogenetic and genotypic analysis revealed that the strains were separated from the species of Mucilaginibacter described to date. Therefore, strains Gh-67T and Gh-48T represent novel species of Mucilaginibacter, for which we propose the names Mucilaginibacter gossypii sp. nov. (type strain Gh-67T =NCIMB 14470T =KCTC 22380T) and Mucilaginibacter gossypiicola sp. nov. (type strain Gh-48T =NCIMB 14471T =KCTC 22379T).

scholarly journals Effect of Arbuscular Mycorrhizal (AM) fungi on growth enhancement of black pepper (Piper nigrum L.) at nursery stage

2019 ◽  
Vol 15 (1) ◽  
pp. 137-140
Author(s):  
S.B. Gurumurthy ◽  
S.V. Patil ◽  
T.H. Shankarappa ◽  
A. Prashant ◽  
Ratnakar M. Shet
Keyword(s):  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Growth Enhancement

scholarly journals Effect of biofertilizers and organic supplements on the growth of black pepper rooted cuttings (Piper nigrum L.)

1970 ◽  
pp. 54
Author(s):  
T S Aswathy, M K Dhanya, J Johny, T Sathyan, T T Preethy, M Murugan
Keyword(s):  
Humic Acid ◽  
Plant Growth Promoting Rhizobacteria ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Research Station ◽  
Rooted Cuttings ◽  
Plant Growth Promoting ◽  
Number Of Leaves ◽  
Growth Promoting ◽  
Vegetative Characters

An experiment was conducted at the Cardamom Research Station, Kerala Agricultural University, Pampadumpara (Kerala) with an objective to study the effect of different biofertilizers (Phosphorus solubilizing bacteria, Azospirillum and Plant Growth Promoting Rhizobacteria Mix I) and organic supplements (fish extract and humic acid) on the growth of black pepper rooted cuttings. The results of the experiment indicated that application of Phosphorus solubilizing bacteria (5 g) along with Azospirillum (5 g), humic acid (0.2%) and fish extract (0.5%) was the best combination for the production of black pepper rooted cuttings with improved vegetative characters (plant height, number of leaves, number of roots, length of roots and leaf area) compared to theirindividual inoculation.This innovative information can be effectively utilized and advocated for the commercial production of black pepper rooted cuttings with lusty growth.

scholarly journals Pontibacter niistensis sp. nov., isolated from forest soil

2010 ◽  
Vol 60 (12) ◽  
pp. 2867-2870 ◽  
Author(s):  
Syed G. Dastager ◽  
Q. S. Raziuddin ◽  
C. K. Deepa ◽  
Wen-Jun Li ◽  
Ashok Pandey
Keyword(s):  
16S Rrna ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Sequence ◽  
Dense Forest ◽  
Western Ghats Of India ◽  
Type Strains ◽  
The Western Ghats

A Gram-negative, rod-shaped bacterial strain, NII-0905T, that was motile by gliding was isolated from soil of a dense forest collected from the Western Ghats of India and its taxonomic position was established. Strain NII-0905T contained MK-7 as the major menaquinone and anteiso-C17 : 0, anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major cellular fatty acids. The DNA G+C content of strain NII-0905T was 51.47 mol%. 16S rRNA gene sequence-based phylogenetic analysis confirmed the placement of strain NII-0905T in the genus Pontibacter and strain NII-0905T exhibited 93.9–96.3 % 16S rRNA sequence similarity with type strains of species of the genus Pontibacter. On the basis of genotypic and phenotypic evidence, strain NII-0905T is considered to represent a novel species of the genus Pontibacter, for which the name Pontibacter niistensis sp. nov. is proposed. The type strain is NII-0905T (=NCIM 5339T =CCTCC AA 209057T).

scholarly journals Biocontrol and growth promotive potential of Streptomyces spp. in black pepper (Piper nigrum L.)

2017 ◽  
Vol 30 (3) ◽  
pp. 177 ◽  
Author(s):  
R. Suseela Bhai ◽  
A. Lijina ◽  
T. P. Prameela ◽  
P. B. Krishna ◽  
Anushree Thampi
Keyword(s):  
Dehydrogenase Activity ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Sequence Similarity ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Root Weight ◽  
Foot Rot ◽  
Challenge Inoculation ◽  
Slow Decline

Actinomycetes isolated from the rhizosphere of black pepper and from vermicompost were tested for their antagonistic effect against <em>Phytophthora capsici</em> and <em>Radopholus similis</em>, the causal agents of foot rot and slow decline diseases of black pepper. Based on <em>in vitro</em> evaluations, four isolates were shortlisted (IISR Act2, IISR Act5, IISR Act6, and IISR Act9) and subjected to<em> in vivo</em> evaluation for <em>Phytophthora</em> infection by challenge inoculation and also greenhouse evaluation for growth promotion in black pepper. Rooted plants of black pepper were raised in soil amended with Actinomycetes strains individually and in combinations in portray and were transplanted into earthenware pots containing potting mixture amended with respective actinomycetes keeping un-amended plants as control. Observations were recorded on growth parameters like plant height, root weight, shoot weight and root infection by nematodes. Besides, soil was also analyzed for pH, dehydrogenase activity, EC and NPK content to know the influence of actinomycetes on soil microflora as well as on nutrient status. The results showed that consortia are more effective than individual isolates. Consortia holding IISR Act5+IISR Act9 were found highly effective in enhancing all the growth parameters followed by IISR Act2+ IISR Act9 and IISR Act2 + IISR Act5. The dehydrogenase activity was found higher in these consortia showing the higher microbial metabolic activity. Root lesions were also negligible in these treatments. Being effective in growth promotion as well as antagonistic activity, the isolates were tested for plant growth promotion and biocontrol traits. Among the isolates, IISR Act9 was found highly efficient in IAA production (119μg/ml) when compared to IISR Act2 (36.25μg/ml) and IISR Act5 (32.4μg/ml). Hence based on the growth promotive and pathogen suppressive effect, the consortia of either IISR Act5+IISR Act9 , IISR Act2+IISR Act9 or IISR Act2+IISR Act5 can be effectively used in black pepper for growth promotion and biological control of foot rot and slow decline diseases. The potential actinomycetes were identified as <em>Streptomyces</em> spp. as per Bergey’s manual and rpoB gene sequence similarity of which IISR Act2 is identified as <em>Streptomyces</em> sp., IISR Act9 as <em>Streptomyces</em> <em>albus</em> and IISR Act5 as <em>Streptomyces</em> sp.

Antileishmanial Alkaloids from Black Pepper (Piper nigrum)

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
HRW Dharmaratne ◽  
BL Tekwani ◽  
NPD Nanayakkara
Keyword(s):  
Black Pepper ◽  
Piper Nigrum
Sign in / Sign up

Export Citation Format

Share Document