Genetic characterization of Meloidogyne incognita isolates from Turkey using sequence-related amplified polymorphism (SRAP)

Biologia ◽  
2012 ◽  
Vol 67 (3) ◽  
Author(s):  
Zübeyir Devran ◽  
Ömür Baysal
Keyword(s):  
Genetic Variation ◽  
Meloidogyne Incognita ◽  
Cost Effective ◽  
Nematode Species ◽  
Root Knot Nematode ◽  
Breeding Programs ◽  
Widespread Species ◽  
Resistant Varieties ◽  
Southern Root Knot Nematode

AbstractSouthern root knot nematode Meloidogyne incognita is the most widespread-species, causing serious yield losses in protected vegetables fields in the West Mediterranean region of Turkey. The knowledge of genetic variation within M. incognita is required for disease management and improvement of resistant varieties by breeding programs. In the present study, the isolates were classified into different groups based on sequence-related amplified polymorphism (SRAP) fingerprints. To our knowledge, this is the first study carried out on the characterization of M. incognita isolates using SRAP. The schematic diagram by tested primers to differentiate of M. incognita isolates was formed in discrimination of nematodes as an effective molecular tool since it is cost effective and easiness. Data presents a genetic variation on root-knot nematode species. These selected SRAP markers can be used to follow genetic structure and differentiation on M. incognita isolates in a certain region.

scholarly journals Controlling the southern root-knot nematode (Meloidogyne incognita Chitwood) with grafted and resistant pepper varieties

2010 ◽  
Vol 16 (2) ◽  
Author(s):  
Z. Mándoki
Keyword(s):  
Meloidogyne Incognita ◽  
Control Method ◽  
Environmentally Friendly ◽  
Bell Pepper ◽  
N Gene ◽  
Root Knot Nematode ◽  
Resistant Varieties ◽  
Environmentally Safe ◽  
Resistant Rootstock ◽  
Southern Root Knot Nematode

Newly bred resistant bell pepper varieties and those grafted onto resistant rootstock s were tested in soil severely infested with southern root-knot nematode [Meloidogy11e incognita (Kofoid and White) Chitwood] in unheated plastic house and compared to varieties on their own roots, in order to evaluate the efficiency of this environmentally friendly control method. 'Cinema F I ' carrying the N gene yielded significantly more than the two susceptible varieties. Varieties grafted onto resistant rootstocks outyielded those on their own roots although to different extent, which was not always significant. At the end of the vegetat ion period the roots of the rootstocks were undamaged and the roots of some resistant varieties were slightly infected. whereas the roots of susceptible varieties were severely damaged. According to our result  . both the use of resistant varieties and grafted plants offer an effective and environmentally safe way of controlling M. incognita.

scholarly journals CHARACTERIZATION OF RESISTANCE TO ROOT-KNOT NEMATODES IN SWEETPOTATO

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 868e-869
Author(s):  
J.A. Thies
Keyword(s):  
Meloidogyne Incognita ◽  
Ipomoea Batatas ◽  
Nematode Species ◽  
Plant Introduction ◽  
Root Knot Nematode ◽  
Race 1 ◽  
Race 2 ◽  
The U.S ◽  
Greenhouse Tests

Thirteen sweetpotato (Ipomoea batatas) genotypes were characterized for resistance to Meloidogyne incognita, M. javanica, M. hapla, and M. arenaria races 1 and 2 in greenhouse tests. The following sweetpotato genotypes representing a range of reactions to M. incognita were evaluated: U.S. Plant Introduction (PI) 399163 (highly resistant = HR), Sumor (HR), Nemagold (HR), Excel (HR), Tinian (HR), Hernandez (resistant = R), Jewel (R), Regal (R), Porto Rico (intermediate = I), Centennial (susceptible = S), Georgia Jet (S), Sulfur (S), and Beauregard (S). Meloidogyne incognita was most pathogenic to sweetpotato of the four Meloidogyne spp. evaluated in these studies. The U.S. Plant Introduction (PI) 399163 and Sumor were resistant to M. incognita in all tests. Only two genotypes, Beauregard and Porto Rico, were susceptible to M. javanica. All genotypes evaluated were resistant to M. hapla, M. arenaria race 1, and M. arenaria race 2. Sumor, U.S. PI 399163, and Nemagold appear to provide the highest levels of resistance against the four Meloidogyne spp. used in these studies. Since M. incognita is the most commonly occurring root-knot nematode species in sweetpotato growing areas of the southern U.S. and is pathogenic to most of the commonly grown sweetpotato cultivars, efforts to develop resistant cultivars that have desirable horticultural characteristics for the U.S. market should be directed toward this root-knot nematode species.

Inheritance of Resistance to the Southern Root-knot Nematode in Capsicum chinense Jacq.

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 500d-500
Author(s):  
R.L. Fery ◽  
J.A. Thies
Keyword(s):  
Meloidogyne Incognita ◽  
Dominant Gene ◽  
Nematode Resistance ◽  
Inheritance Of Resistance ◽  
Capsicum Chinense ◽  
Root Knot Nematode ◽  
Breeding Programs ◽  
Backcross Populations ◽  
Southern Root Knot Nematode ◽  
Greenhouse Tests

A series of greenhouse tests were conducted to compare the level of resistance to the southern root-knot nematode (Meloidogyne incognita) exhibited by recently released Capsicum chinense germplasm lines to the level of resistance exhibited by C. annuum cultivars, to determine the inheritance of the resistance in C. chinense, and to determine genetic relationship between the resistances exhibited by C. chinense and C. annuum. The results of a replicated test indicated that the level of resistances exhibited by the recently released C. chinense germplasm lines PA-353, PA-398, and PA-426 is equal to the level of resistances exhibited by the resistant C. annuum cultivars Mississippi Nemaheart and Carolina Cayenne. Evaluation of parental, F1, F2, and backcross populations of the cross PA-426 × PA-350 indicated that the resistance in C. chinense is conditioned by a single dominant gene. The results of an allelism test indicated that this dominant gene is allelic to the N gene that conditions southern root-knot nematode resistance in the C. annuum cultivar Carolina Cayenne. The availability of a simply inherited source of outstanding resistance makes breeding for southern root-knot nematode resistance a viable objective in C. chinense breeding programs. This objective should be readily obtained by the application of conventional plant breeding methodologies.

Identification of four populations of Meloidogyne incognita in Georgia, USA capable of parasitizing tomato bearing Mi-1.2 gene

Plant Disease ◽  
2021 ◽  
Author(s):  
Abolfazl Hajihassani ◽  
Josiah Marquez ◽  
Moges Woldemeskel ◽  
Negin Hamidi
Keyword(s):  
Meloidogyne Incognita ◽  
Nematode Species ◽  
The United States ◽  
Egg Laying ◽  
Female Development ◽  
Root Knot Nematode ◽  
Resistant Cultivars ◽  
History Of ◽  
Tomato Cultivars ◽  
Southern Root Knot Nematode

Meloidogyne incognita, the southern root-knot nematode (RKN), is the most predominant plant-parasitic nematode species of tomato and causes significant yield loss. The Mi-1.2 gene confers resistance in tomatoes to M. incognita; however, virulent RKN populations capable of parasitizing resistant tomato cultivars have been reported from different regions in the world. Four naturally occurring virulent populations of M. incognita were found in vegetable fields from four counties in Georgia with no history of tomato cultivation of the Mi gene. Two consecutive greenhouse trials showed that all four virulent RKN populations reproduced on tomato cultivars, including Amelia, Skyway, and Myrtle with the Mi-1 gene, while an avirulent population of M. incognita race 3 was unable to overcome host resistance. Virulent RKN populations varied in reproduction among resistant cultivars, with Ma6 population having the greatest reproduction potential. No difference in penetration potential of the virulent (Ma6) and avirulent populations were found on susceptible and resistant tomato cultivars. However, virulent Ma6 population females were successful at egg-laying, while the avirulent female development was arrested in the resistant cultivars. The virulent Ma6 population also induced feeding sites in the roots of resistant cultivars, while the avirulent population did not. To our knowledge, this is the first report of resistance-breaking populations of M. incognita in Georgia and the second state in the United States after California.

scholarly journals Genetic Analysis of Resistance to the Southern Root-knot Nematode in Capsicum chinense Jacq.

1998 ◽  
Vol 123 (6) ◽  
pp. 1008-1011 ◽  
Author(s):  
Richard L. Fery ◽  
Judy A. Thies
Keyword(s):  
Meloidogyne Incognita ◽  
Dominant Gene ◽  
Nematode Resistance ◽  
Capsicum Chinense ◽  
Root Knot Nematode ◽  
Breeding Programs ◽  
Germplasm Line ◽  
Backcross Populations ◽  
Southern Root Knot Nematode ◽  
Greenhouse Tests

Greenhouse tests were conducted to compare the levels of resistance to the southern root-knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] exhibited by recently released Capsicum chinense Jacq. Scotch Bonnet-type germplasm lines PA-353, PA-398, and PA-426 to the levels of resistance exhibited by C. annuum L. `Carolina Cayenne' and `Mississippi Nemaheart'; to determine the inheritance of the resistance in C. chinense germplasm line PA-426; and to determine the genetic relationship between the resistances exhibited by C. chinense germplasm line PA-426 and C. annuum `Carolina Cayenne'. The results of a replicated test indicated that the level of resistances exhibited by the resistant released C. chinense germplasm lines is equal to the level of resistances exhibited by the resistant C. annuum cultivars. Evaluation of parental, F1, F2, and backcross populations of the cross PA-426 × PA-350 (a susceptible Habanero-type C. chinense cultigen) indicated that the resistance in C. chinense is conditioned by a single dominant gene. The results of an allelism test indicated that this dominant gene is allelic to the dominant gene that conditions much of the southern root-knot nematode resistance in the C. annuum `Carolina Cayenne'. The ease and reliability of evaluating plants for resistance to root-knot nematode and the availability of a simply inherited source of outstanding resistance makes breeding for southern root-knot nematode resistance a viable objective in C. chinense breeding programs.

Phenotypic and genotypic characterization of tomato genotypes for resistance to root-knot nematode, Meloidogyne incognita

2020 ◽  
Vol 100 (1) ◽  
pp. 28
Author(s):  
Anupam ◽  
Narpinderjeet Kaur Dhillon ◽  
Sukhjeet Kaur ◽  
Salesh Kumar Jindal ◽  
Harwinder Singh Buttar
Keyword(s):  
Meloidogyne Incognita ◽  
Root Knot Nematode ◽  
Genotypic Characterization ◽  
Tomato Genotypes ◽  
Phenotypic And Genotypic Characterization

scholarly journals Characterization of siRNAs clusters in Arabidopsis thaliana galls induced by the root-knot nematode Meloidogyne incognita

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Clémence Medina ◽  
Martine da Rocha ◽  
Marc Magliano ◽  
Alizée Raptopoulo ◽  
Nathalie Marteu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Meloidogyne Incognita ◽  
Root Knot Nematode

scholarly journals Screening and Histopathological Characterization of Korean Carrot Lines for Resistance to the Root-Knot Nematode Meloidogyne incognita

2014 ◽  
Vol 30 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Yunhee Seo ◽  
Jiyeong Park ◽  
Yong Su Kim ◽  
Yong Park ◽  
Young Ho Kim
Keyword(s):  
Meloidogyne Incognita ◽  
Root Knot Nematode

Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasitic juveniles of the southern root-knot nematode Meloidogyne incognita

Nematology ◽  
2001 ◽  
Vol 3 (2) ◽  
pp. 129-139 ◽  
Author(s):  
Jaap Bakker ◽  
Fred Gommers ◽  
Geert Smant ◽  
Pierre Abad ◽  
Marie-Noëlle Rosso ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cdna Library ◽  
Meloidogyne Incognita ◽  
Sequence Data ◽  
Homo Sapiens ◽  
Large Fraction ◽  
Nematode Species ◽  
Cdna Libraries ◽  
Parasitic Nematodes ◽  
Root Knot Nematode

AbstractExpressed sequence tags (EST) have been widely used to assist in gene discovery in various organisms (e.g., Arabidopsis thaliana, Caenorhabditis elegans, Mus musculus, and Homo sapiens). In this paper we describe an EST project, which aims to investigate gene expression in Meloidogyne incognita at the onset of parasitism. Approximately 1000 5′-end sequence tags were produced from a cDNA library made of freshly hatched preparasitic second stage juveniles (J2). The EST were identified in the primary transformants of the cDNA library, and assigned to nine different functional groups, including (candidate) parasitism genes. A large fraction of the EST (45%) did not have a putative homologue in public databases. Sixty five percent of the EST that could be clustered into a functional group had putative homologues in other nematode species. EST were found for virtually all parasitism related genes that have been cloned from M. incognita to date. In addition, several novel genes were tagged, including a xylanase and a chitinase gene. The efficiency of EST projects, which produce sequence data for thousands of genes in months time without any difficult pre-selections of mRNA pools, makes random sequencing cDNA libraries a superior method to identify candidates for parasitism related genes in plant-parasitic nematodes. The sequences in this paper are retrievable from Genbank with the accession numbers BE191640 to BE191741, BE217592 to BE217720, BE225324 to BE225598, BE238852 to BE239221, and BE240829 to BE240865.

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