Effects of sugars on the growth and chlorophyll content in excised tulip stem in the presence of Indole-3-acetic acid

ABSTRACT The purpose of this study was to clarify the effect of sucrose on auxin-induced growth of stem excised from growing tulips and excised directly from cooled and not cooled bulbs, and on the growth of excised IV internode from growing plants in the presence of auxin. In all cases flower bud was replaced by IAA (indole-3- acetic acid, 0.1%, w/w in lanolin) and basal part of excised segments of stem was kept in distilled water or in solution of various sugars at different concentrations. IAA-induced growth of excised stems isolated from growing tulips was inhibited by sucrose at concentrations of 5.0% and 10.0%, but sucrose at 1.25% and 2.5% did not. Sucrose at all concentrations used evidently delayed senescence and increased chlorophyll contents in excised stems in the presence of IAA. Sucrose induced stiffing in isolated stems in the presence of IAA, and much less infective by pathogen in comparison to stem treated with IAA only. Mannitol and sorbitol at concentrations of 5.0% and 10.0% substantially inhibited IAA-induced growth of stem segments. Stem segments excised from cooled and not cooled tulip bulbs were more sensitive than those isolated from growing shoots due to application of sucrose and glucose; more inhibitory effect was observed. Sucrose at concentrations of 5.0% and 10.0% only slightly inhibited growth of IV internode treated with IAA and all concentrations of sucrose (1.25%, 2.5%, 5.0% and 10.0%) substantially increased chlorophyll content. The possible mode of actions of sucrose interacting with auxin to regulate stem growth is also discussed although sugar response is complicated by the fact that plants have multiple sugar-response pathways.

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Rooting response of etiolated stem segments ofPopulus nigra to antimetabolites in relation to indole-3-acetic acid and glucose

Biologia Plantarum ◽

10.1007/bf02922761 ◽

1973 ◽

Vol 15 (6) ◽

pp. 412-418 ◽

Cited By ~ 9

Author(s):

K. K. Nanda ◽

M. K. Jain ◽

N. C. Bhattacharya

Keyword(s):

Acetic Acid ◽

Indole 3 Acetic Acid ◽

Rooting Response ◽

Stem Segments

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The acropetal effects of indole-3-acetic acid in isolated shoot segments of Acer pseudoplatanus L. II. Possible regulation by a vectorial fieid of auxin waves

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1991.003 ◽

2014 ◽

Vol 60 (1-2) ◽

pp. 51-66

Author(s):

Jacek A. Adamczyk

Keyword(s):

Acetic Acid ◽

Phase Shift ◽

Direct Action ◽

Lateral Branch ◽

Stem Segment ◽

Acer Pseudoplatanus ◽

Basipetal Transport ◽

Indole 3 Acetic Acid ◽

Stem Segments ◽

Growing Shoot

The acropetal effects of auxin on elongation of axillary buds and on modulation of the wave-like pattern of basipetal efflux of natural auxin to agar from Acer pseudoplatanus L. shoots were studied. When synthetic IAA was applied to cut surfaces of one of two branches the elongation growth of buds situated on the opposite branch was retarded, suggesting regulation independent of the direct action of the molecules of the applied IAA. Oscillations in basipetal transport of natural auxin along the stem segments were observed corroborating the results of other authors using different tree species. Apical application of synthetic IAA for 1 hour to the lateral branch caused a phase shift of the wave-like pattern of basipetal efflux of natural auxin, when the stem segment above the treated branch was sectioned. The same effect was observed evoked by the laterally growing branch which is interpreted as an effect of natural auxin produced by the actively growing shoot. These modulations could be propagated acropetally at a rate excluding direct action of auxin molecules at the sites of measurement. The results seem to corroborate the hypothesis suggesting that auxin is involved in acropetal regulation of shoot apex growth through its effect upon modulation of the vectorial field which arises when the auxin-waves translocate in cambium.

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Interaction between Aspterric Acid and Indole-3-acetic Acid on Reproductive Growth in Arabidopsis thaliana

Zeitschrift für Naturforschung C ◽

10.1515/znc-2005-7-810 ◽

2005 ◽

Vol 60 (7-8) ◽

pp. 572-576 ◽

Cited By ~ 5

Author(s):

Atsumi Shimada ◽

Hisakazu Yamane ◽

Yasuo Kimura

Keyword(s):

Arabidopsis Thaliana ◽

Acetic Acid ◽

Pollen Development ◽

Growth Inhibitor ◽

Stem Length ◽

Flower Bud ◽

Reproductive Growth ◽

Normal Pollen ◽

Pollen Growth ◽

Indole 3 Acetic Acid

Application of indole-3-acetic acid (IAA) with a pollen growth inhibitor, aspterric acid (AA), results in the recovery of normal pollen development. In contrast, application of gibberellin (GA3) with AA do not induce normal pollen growth. In addition, application of different concentrations of IAA with AA shortens the period of growth from bolting to first flowering as compared to that treated with AA alone. Furthermore, stem length and number of flower bud treated with IAA and AA were similar to those of control. These results suggest, that IAA may play an important role in reproductive growth of A. thaliana.

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Utilization of the Plant Hormone Indole-3-Acetic Acid for Growth by Pseudomonas putida Strain 1290

Applied and Environmental Microbiology ◽

10.1128/aem.71.5.2365-2371.2005 ◽

2005 ◽

Vol 71 (5) ◽

pp. 2365-2371 ◽

Cited By ~ 139

Author(s):

Johan H. J. Leveau ◽

Steven E. Lindow

Keyword(s):

Acetic Acid ◽

Pseudomonas Putida ◽

Sole Source ◽

Inhibitory Effect ◽

16S Rrna Sequence ◽

Associated Bacteria ◽

Catechol 1,2 Dioxygenase ◽

Positive Effect ◽

Indole 3 Acetic Acid ◽

Exogenous Iaa

ABSTRACT We have isolated from plant surfaces several bacteria with the ability to catabolize indole-3-acetic acid (IAA). One of them, isolate 1290, was able to utilize IAA as a sole source of carbon, nitrogen, and energy. The strain was identified by its 16S rRNA sequence as Pseudomonas putida. Activity of the enzyme catechol 1,2-dioxygenase was induced during growth on IAA, suggesting that catechol is an intermediate of the IAA catabolic pathway. This was in agreement with the observation that the oxygen uptake by IAA-grown P. putida 1290 cells was elevated in response to the addition of catechol. The inability of a catR mutant of P. putida 1290 to grow at the expense of IAA also suggests a central role for catechol as an intermediate in IAA metabolism. Besides being able to destroy IAA, strain 1290 was also capable of producing IAA in media supplemented with tryptophan. In root elongation assays, P. putida strain 1290 completely abolished the inhibitory effect of exogenous IAA on the elongation of radish roots. In fact, coinoculation of roots with P. putida 1290 and 1 mM concentration of IAA had a positive effect on root development. In coinoculation experiments on radish roots, strain 1290 was only partially able to alleviate the inhibitory effect of bacteria that in culture overproduce IAA. Our findings imply a biological role for strain 1290 as a sink or recycler of IAA in its association with plants and plant-associated bacteria.

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Effect of indole-3-acetic acid (IAA) and sucrose on vessel size and density in isolated stem segments of oak (Quercus robur)

Physiologia Plantarum ◽

10.1111/j.1399-3054.1991.tb02135.x ◽

1991 ◽

Vol 81 (2) ◽

pp. 234-238 ◽

Cited By ~ 15

Author(s):

Jacek Zakrzewski

Keyword(s):

Acetic Acid ◽

Quercus Robur ◽

Vessel Size ◽

Indole 3 Acetic Acid ◽

Stem Segments

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9-(3,4-Dimethyl-5-pentyl-furan-2-yl) nonanoic Acid and 9-(3,4-DimethyI-5-propyl-furan-2-yl) nonanoic Acid: New Naturally Occurring Peroxidase Inhibitors

Zeitschrift für Naturforschung C ◽

10.1515/znc-1999-1112 ◽

1999 ◽

Vol 54 (11) ◽

pp. 932-936 ◽

Cited By ~ 3

Author(s):

Claus T. Fuchs ◽

Gerhard Spiteller

Keyword(s):

Acetic Acid ◽

Horseradish Peroxidase ◽

Inhibitory Activity ◽

Methyl Esters ◽

Inhibitory Effect ◽

Side Chain ◽

Nonanoic Acid ◽

Naturally Occurring ◽

Indole 3 Acetic Acid

Abstract 9-(3,4-Dimethyl-5-pentyl-furan-2-yl) nonanoic acid [diMeF(9,5)] and 9-(3,4-dimethyl-5-propyl-furan-2-yl) nonanoic acid [diMeF(9,3)] and its corresponding methyl esters have been assayed for inhibitory activity on horseradish peroxidase (E C 1.11.1.17) by measuring the peroxidase-catalyse decomposition of indole-3-acetic acid. Both compounds and their meth-ylates are com petitive inhibitors to horseradish peroxidase with inhibitor constants (K1) of 50 ± 0.9 × 10-5 ᴍ respectively 5.2 ± 0.8 × 10-5 ᴍ. Development of inhibitory effect requires not only the presence of the furan heterocycle but also of a polar side chain.

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Temporal and Spatial Pattern of Indole-3-acetic Acid Occurrence during Walnut Pistillate Flower Bud Differentiation as Revealed by Immunohistochemistry

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.137.5.283 ◽

2012 ◽

Vol 137 (5) ◽

pp. 283-289 ◽

Cited By ~ 3

Author(s):

Ying Gao ◽

Hao Liu ◽

Ningguang Dong ◽

Dong Pei

Keyword(s):

Acetic Acid ◽

Spatial Pattern ◽

Morphological Changes ◽

Floral Induction ◽

Juglans Regia ◽

Pistillate Flower ◽

Flower Bud ◽

Flower Bud Differentiation ◽

Temporal And Spatial ◽

Indole 3 Acetic Acid

We used anti-indole-3-acetic acid (IAA) monoclonal antibodies to monitor the temporal and spatial pattern of IAA during pistillate flower bud differentiation in the walnut (Juglans regia) cultivar Liaoning 1. Based on morphological changes, the process of pistillate flower bud differentiation was divided into five stages. The flower induction stage, which includes the early phase, midphase, and late phase, persisted from 25 Apr. to the end of May. The pedicel differentiation stage began on 5 June. The bract primordium stage began on 25 June and persisted through mid-March of the next year. Both the perianth and pistil differentiation stages persisted for nearly 2 weeks. During the floral induction period, little IAA was present in the shoot apical meristem (SAM); hence, the SAM may not always be a site of IAA production. IAA was obviously concentrated in cells of the first several layers of the SAM during pedicel primordium formation. High levels of IAA were also noted in the phyllome, young leaf tips, and vascular bundle of leaves and gemmae. This direct evidence indicates that no close relationship exists between IAA and physiological differentiation; instead, IAA may strongly affect morphogenesis. These findings comprise a first step toward elucidating the walnut flowering mechanism.

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Hormonal regulation of the growth of leaves and inflorescence stalk in Muscari armeniacum Leichtl.

Acta Agrobotanica ◽

10.5586/aa.1654 ◽

2016 ◽

Vol 69 (1) ◽

Author(s):

Marian Saniewski ◽

Justyna Góraj-Koniarska ◽

Elżbieta Węgrzynowicz-Lesiak ◽

Eleonora Gabryszewska

Keyword(s):

Acetic Acid ◽

Abscisic Acid ◽

Plant Growth ◽

Gibberellic Acid ◽

Growth Regulators ◽

Hormonal Regulation ◽

Present Experiment ◽

Stem Growth ◽

Flower Bud ◽

Indole 3 Acetic Acid

It is known that chilling of Muscari bulbs is necessary for the growth of the inflorescence stalk and flowering, but not for the growth of leaves. Gibberellic acid (GA) accelerated stem growth and flowering in chilled Muscari bulbs. In the present experiment it was shown that in unchilled derooted Muscari bulbs the growth of leaves, but not the growth of the inflorescence stalk, was observed when bulbs were stored in water, GA at a concentration of 50 and 100 mg/L, benzyladenine (BA) at a concentration of 25 and 50 mg/L, or a mixture of GA+BA (50+25 mg/L), but abscisic acid (ABA) at a concentration of 10 mg/L greatly inhibited the growth of leaves. In chilled derooted Muscari bulbs the growth of leaves and inflorescence stalk was observed when bulbs were stored in water or GA, but BA and GA+BA treatments totally inhibited the growth of the inflorescence stalk without an effect on the growth of leaves. These results clearly showed that the growth of leaves and inflorescence stalk in Muscari bulbs are controlled by plant growth regulators in different ways. ABA totally inhibited the growth of leaves and inflorescence stalk in chilled derooted Muscari bulbs. It was shown that after the excision of the inflorescence bud in cultivated chilled Muscari bulbs, the inflorescence stalk died, but application of indole-3-acetic acid (IAA) 0.5% in the place of the removed inflorescence bud induced the growth of the inflorescence stalk. IAA applied under the inflorescence bud inhibited the development of flowers (flower-bud blasting) and induced the growth of the inflorescence stalk below the treatment site. These results are discussed with reference to hormonal regulation of stem (stalk) growth in tulip, narcissus, hyacinth, and Hippeastrum.

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