scholarly journals Bazı Turunçgil Anaçlarında Tuza Tolerans için In vitro Testleme

2020 ◽  
Vol 8 (5) ◽  
pp. 1117-1121
Author(s):  
Mehmet Yaman ◽  
Hasan Pınar ◽  
Ubeyit Seday ◽  
Duygu Altınöz ◽  
Aydın Uzun ◽  
...  
Keyword(s):  
Poncirus Trifoliata ◽  
Sour Orange ◽  
Development Programs ◽  
Trifoliate Orange ◽  
Rough Lemon ◽  
Cultivar Development ◽  
Volkamer Lemon ◽  
Citrus Volkameriana ◽  
Cleopatra Mandarin

Just because of geographical spread, citrus species generally grow in places sensitive to salinity. Testing methods have a significant role in breeding and cultivar development programs. This study was conducted to investigate in vitro salt response of Cleopatra mandarin (Citrus reshni Tan.), sour orange (Citrus aurantium L.), rough lemon (Citrus jambhiri Lush.), Volkamer lemon (Citrus volkameriana Tan & Pasq.), Carrizo citrange (Poncirus trifoliata L. Raf. X Citrus sinensis L. Osbeck) and trifoliate orange (Poncirus trifoliata Raf.) rootstocks at different NaCl concentrations. Seeds were germinated in MS medium with 0, 45, 90 and 135 mM NaCl concentrations. In general, the greatest germination rates in all salt concentrations in Volkamer lemon and sour orange rootstocks and the lowest values were observed in rough lemon and trifoliate orange rootstocks. Present findings revealed that in vitro conditions could reliably be used in salt tolerance tests of citrus rootstocks.

OXIDATIVE ENZYME RESPONSES OF SIX CITRUS ROOTSTOCKS INFECTED WITH PHOMA TRACHEIPHILA (PETRI) KANTSCHAVELI AND GIKASHVILI

2012 ◽  
Vol 48 (4) ◽  
pp. 563-572 ◽  
Author(s):  
AYDIN UZUN ◽  
UBEYIT SEDAY ◽  
ERCAN CANIHOS ◽  
OSMAN GULSEN
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzyme Activity ◽  
Poncirus Trifoliata ◽  
Citrus Limon ◽  
Sour Orange ◽  
Trifoliate Orange ◽  
Rough Lemon ◽  
Phoma Tracheiphila ◽  
Cleopatra Mandarin ◽  
Mal Secco

SUMMARYCitrus trees are often exposed to severe infectious diseases. Mal secco caused by Phoma tracheiphila (Petri) Kantschaveli and Gikashvili is one of the most destructive fungal diseases of lemons (Citrus limon Burm. F.). In the present study, antioxidant enzyme activity in different mal secco-resistant and susceptible citrus rootstocks including Cleopatra mandarin (C. reshni Tan.), sour orange (C. aurantium L.), rough lemon (C. jambhiri Lush.), Volkameriana (C. volkameriana Tan. and Pasq.), Carrizo citrange (Poncirus trifoliata L. Raf. X C. sinensis L. Osbeck) and trifoliate orange (P. trifoliata) was investigated. Possible differences in constitutive levels of these antioxidant enzymes and correlations between enzyme levels and mal secco caused by P. tracheiphila were examined. Among the rootstocks, Cleopatra mandarin was found to be resistant to mal secco, whereas rough lemon, sour orange and trifoliate orange were highly susceptible. Total peroxidase (TPX; EC: 1.11.1.7) activity increased in all infected rootstocks. Ascorbate peroxidase (APX; EC: 1.11.1.11) activity increased in most of the rootstocks and no correlation was found between catalase (CAT; EC: 1.11.1.6) activity and mal secco resistance. This study indicates that overall TPX activity is upregulated and APX activity is up- and down-regulated depending on the type of rootstock in response to P. tracheiphila infection.

scholarly journals Rootstocks for 'Tahiti' lime

2004 ◽  
Vol 61 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Neusa Maria Colauto Stenzel ◽  
Carmen Silvia Vieira Janeiro Neves
Keyword(s):  
Block Design ◽  
Fruit Weight ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Canopy Development ◽  
Rangpur Lime ◽  
Rough Lemon ◽  
Randomized Block Design ◽  
Volkamer Lemon ◽  
Cleopatra Mandarin

The 'Tahiti' lime (Citrus latifolia Tanaka) is an important commercial citrus cultivar in Brazil. 'Rangpur' lime has being used as its main rootstock, but it is susceptible to root rot caused by Phytophthora, reducing tree longevity. An experiment was set up in a randomized block design, with three trees per plot of each rootstock and four replicates, and run for 12 years, aiming to compare the performance of 'IAC-5 Tahiti' lime, budded on 'Rangpur' lime (Citrus limonia Osb.); 'C-13' citrange (Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.); 'African' rough lemon (Citrus jambhiri Lush.); 'Volkamer' lemon (Citrus volkameriana Ten. & Pasq.); trifoliate orange (Poncirus trifoliata (L.) Raf.); 'Sunki' mandarin (Citrus sunki Hort. ex Tan.) and 'Cleopatra' mandarin (Citrus reshni Hort. ex Tan.). Eleven years after the establishment of the orchard, trees with the greatest canopy development were budded on 'C-13' citrange and 'African' rough lemon, and both differed significantly from trees budded on trifoliate orange, 'Sunki' and 'Cleopatra' mandarins, which presented the smallest canopy development. Trees budded on 'Rangpur' lime and 'C-13' citrange had the highest cumulative yields, and were different from trees budded on trifoliate orange, 'Cleopatra' and 'Sunki' mandarins. There was no rootstock effect on mean fruit weight and on the total soluble solid/acid ratio in the juice. The 'Rangpur' lime and the 'Cleopatra' mandarin rootstocks reduced longevity of plants.

scholarly journals Eight New Somatic Hybrid Citrus Rootstocks with Potential for Improved Disease Resistance

HortScience ◽  
1992 ◽  
Vol 27 (9) ◽  
pp. 1033-1036 ◽  
Author(s):  
Eliezer S. Louzada ◽  
Jude W. Grosseti ◽  
Frederick G. Gmitter ◽  
Beatriz Nielsen ◽  
J.L. Chandler ◽  
...  
Keyword(s):  
Somatic Hybrid ◽  
Somatic Hybrids ◽  
Sweet Orange ◽  
Field Trials ◽  
Poncirus Trifoliata ◽  
Sour Orange ◽  
Rough Lemon ◽  
Hybrid Plants ◽  
Volkamer Lemon ◽  
Cleopatra Mandarin

Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of vigorous tetraploid somatic hybrid plants from eight complementary parental rootstock combinations: Citrus reticulata Blanco (Cleopatra mandarin) + C. aurantium L. (sour orange), C. reticulata (Cleopatra mandarin) + C. jambhiri Lush (rough lemon), C. reticulata (Cleopatra mandarin) + C. volkameriana Ten. & Pasq. (Volkamer lemon), C. reticulata (Cleopatra mandarin) + C. limonia Osb. (Rang-pur), C. sinensis (L.) Osb. (Hamlin sweet orange) + C. limonia (Rangpur), C. aurantium (sour orange) + C. volkameriana (Volkamer lemon) zygotic seedling, C. auruntium hybrid (Smooth Flat Seville) + C. jambhiri (rough lemon), and C. sinensis (Valencia sweet orange) + Carrizo citrange [C. paradisi Macf. × Poncirus trifoliata (L.) Raf.]. Diploid plants were regenerated from nonfused callus-derived protoplasts of Valencia sweet orange and Smooth Flat Seville and from nonfused leaf protoplasts of sour orange, Rangpur, rough lemon, and Volkamer lemon. Regenerated plants were classified according to leaf morphology, chromosome number, and leaf isozyme profiles. All somatic hybrid plants were tetraploid (2n = 4× = 36). One autotetraploid plant of the Volkamer lemon zygotic was recovered, apparently resulting from a homokaryotic fusion. These eight new citrus somatic hybrids have been propagated and entered into field trials.

scholarly journals Horticultural Evaluation of New Citrus latipes Hybrids as Rootstocks for Citrus

HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 595-598 ◽  
Author(s):  
Giuseppe Reforgiato Recupero ◽  
Giuseppe Russo ◽  
Santo Recupero ◽  
Roberto Zurru ◽  
Bruno Deidda ◽  
...  
Keyword(s):  
Fruit Quality ◽  
Female Parent ◽  
Poncirus Trifoliata ◽  
Growth And Yield ◽  
Sour Orange ◽  
Trifoliate Orange ◽  
Plant Yield ◽  
Volkamer Lemon ◽  
Washington Navel ◽  
Swingle Citrumelo

In 1968, the CRA-Research Center for Citriculture and Mediterranean Crops (CRA-ACM) started a research program aimed at breeding citrus rootstocks. The monoembryonic species C. latipes (Swing.) Tan. was used as the female parent; trifoliate orange [Poncirus trifoliata (L.) Raf.], sour orange, and volkamer lemon (C. volkameriana Pasq.) were used as male parents. The behavior of some of these hybrids tested with other standard rootstocks in Sicily and Sardinia was evaluated. The cultivars under comparison included ‘Washington’ navel orange and ‘SRA 92’ clementine in Sardinia and ‘Tarocco’ orange in Sicily. Our results showed the dramatic influence of rootstock on plant growth and yield; only minor effects on fruit quality were observed. Among the standard rootstocks tested, Swingle citrumelo provided the highest yield. Some of the tested hybrids (F5 P12, F6 P12, and F6 P13) may improve plant yield, thus maintaining good fruit quality. Encouraging data obtained with these hybrids may justify the use of monoembryonic species of the Papeda subgenus for breeding citrus rootstocks.

scholarly journals Somatic Hybridization of Complementary Citrus Rootstock:Five New Hybrids

HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 812-813 ◽  
Author(s):  
Jude W. Grosser ◽  
Frederick G. Gmitter ◽  
J.L. Chandler ◽  
Eliezer S. Louzada
Keyword(s):  
Somatic Hybrid ◽  
Somatic Hybridization ◽  
Poncirus Trifoliata ◽  
Sour Orange ◽  
Trifoliate Orange ◽  
Hybrid Plants ◽  
Regenerated Plants ◽  
Citrus Rootstock ◽  
Sexual Hybrid ◽  
Cleopatra Mandarin

Protoplasm culture following polyethylene glycol-induced fusion resulted in the regeneration of tetraploid somatic hybrid plants from the following attempted parental combinations: Cleopatra mandarin (Citrus reticulata Blanco) + Argentine trifoliate orange [Poncirus trifoliata (L.) Raf.]; `Succari' sweet orange [C. sinensis (L.) Osb.] + Argentine trifoliate orange; sour orange (C. aurantium L.) + Flying Dragon trifoliate orange (P. trifolita); sour orange + Rangpur (C. limonia Osb.); and Milam lemon (purported sexual hybrid of C. jambhiri Lush × C. sinensis) + Sun Chu Sha mandarin (C. reticulate Blanco). Protoplasm isolation, fusion, and culture were conducted according to previously published methods. Regenerated plants were classified according to leaf morphology, chromosome number, and peroxidase, phosphoglucomutase, and phosphoglucose isomerase leaf isozyme profiles. All of the somatic hybrid plants were tetraploid, as expected (2n = 4x = 36), and all five selections have been propagated and entered into commercial citrus rootstock trials.

scholarly journals Trunk Xylem Anatomy of Mature Healthy and Blighted Grapefruit Trees on Several Rootstocks

1994 ◽  
Vol 119 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Luiz A.B.C. Vasconcellos ◽  
William S. Castle
Keyword(s):  
Cross Sections ◽  
Citrus Tristeza Virus ◽  
Poncirus Trifoliata ◽  
Vessel Element ◽  
Sour Orange ◽  
Citrus Paradisi ◽  
Xylem Anatomy ◽  
Rough Lemon ◽  
Tristeza Virus ◽  
Cleopatra Mandarin

Wood samples were taken from healthy and blighted citrus trees on several rootstocks to describe and compare the xylem anatomy of the healthy trees and to determine if blight altered xylogenesis. Horizontal trunk xylem cores, 6 cm long, were extracted from blighted 18-year-old commercial grapefruit (Citrus paradisi Macf.) trees on rough lemon (RL) (C. jambhiri Lush.), Cleopatra mandarin (CM) (C. reshni Hort. ex Tan.), and Carrizo citrange (CC) [C. sinensis (L). Osb. × Poncirus trifoliata (L.) Raf] and from healthy trees on those rootstocks and sour orange (SO) (C. aurantium L.). Cores were taken from the eastern and western sides of the scion and rootstock of each tree. The cores were divided into 2-cm pieces and cross-sections were prepared for analysis of vessel element (VE) number and diameter in 0.5-cm increments. A sample-size study showed that tree side was not a significant source of variation and that 10 replications were sufficient to detect differences of ≈12% from the overall mean. Among the healthy trees, VE densities and diameters were similar for the trees on CC or RL and larger than those for trees on SO or CM. VEs were generally smaller and at lower densities in the scion than the rootstock. Few VE occlusions were observed in the healthy trees. In the blighted trees, to a depth of 1 cm, VE density increased and diameter decreased compared to the healthy trees. The largest change occurred in the trees on RL and in the rootstock vs. scion trunk part. The frequency of VE amorphous plugs in blighted trees ranged from 1% to 30%. Similar changes in xylem anatomy were not found in trees with citrus tristeza virus or soilborne pests. Trunk water uptake and dye movement patterns in blighted trees were typical for trees with xylem dysfunction.

scholarly journals Selecting the Best Citrus Rootstock for Young `Valencia' Orange Trees Under Soil Salinity Stress Conditions

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 895E-896
Author(s):  
A.M. Akl*
Keyword(s):  
Salinity Stress ◽  
Soil Salinity ◽  
Sour Orange ◽  
Rough Lemon ◽  
Valencia Orange ◽  
Volkamer Lemon ◽  
Citrus Rootstock ◽  
Orange Trees ◽  
Cleopatra Mandarin ◽  
Troyer Citrange

This study was conducted during 2002 and 2003 seasons to select the best citrus rootstocks for young `Valencia' orange trees grown under soil salinity stress. Six citrus rootstocks namely Volkamer lemon, Cleopatra mandarin, Troyer citrange, Rough lemon, Egyptian lime, and Sour orange were tested. Four soil salinity levels, namely, 0.0%, 0.1%, 0.2%, and 0.4% were examined. Results showed that growth criteria, plant pigments and percentages of N, P, and K in the leaves of `Valencia' orange onto all citrus rootstocks tended to reduce with raising soil salinity. The harmful effects imposed by soil salinity was `Valencia' orange on Volkamer lemon, Cleopatra mandarin Troyer citrange, Rough lemon, Egyptian lime, and Sour orange, in asending order. Results proved the superiority of Volkamer lemon, Cleopatra mandarin, and Trouyer citrange rootstocks for `Valencia' orange transplants grown under salinity conditions as compared to the other rootstocks. `Valencia' orange on such promising rootstocks could tolerate soil salinity until 0.20% without adverse effects on growth of transplants. Sour orange rootstock seems to be the lowest suitable stock for `Valencia' orange tansplants grown under soil salinity conditions.

scholarly journals Effect of Various Rootstocks on the Growth and Yield of Valencia Orange, Marsh Seedless Grapefruit, and Ortanique Trees in Jamaica

1969 ◽  
Vol 60 (4) ◽  
pp. 485-490
Author(s):  
C. C. Weir
Keyword(s):  
Growth And Yield ◽  
Sour Orange ◽  
Trifoliate Orange ◽  
Yield Data ◽  
Rangpur Lime ◽  
Growing Stock ◽  
Rough Lemon ◽  
Valencia Orange ◽  
Cleopatra Mandarin ◽  
Troyer Citrange

The effect of six commercial citrus rootstocks on the growth and yield of Valencia orange, Marsh seedless grapefruit, and Ortanique trees was studied in a rootstock experiment initiated in Jamaica in 1965. Results of these investigations revealed that under the soil and environmental conditions of this experiment, rough lemon and Rangpur lime rootstocks were the most vigorous of the six stocks studied; Cleopatra mandarin was the next most vigorous, while the trifoliate orange was decidedly the least vigorous of the stocks. The widely used sour orange stock was a relatively slow growing stock, and its vigour was about equal to the Troyer citrange rootstock. Yield data taken over the 3-year period 1969 to 1972 showed that the sour orange rootstock produced yields of Valencia orange, Marsh seedless grapefruit, and Ortaniques as good as the more vigorous rough lemon and Rangpur lime rootstocks, and better than the other three stocks, viz., Cleopatra mandarin, Troyer citrange, and trifoliate orange.

scholarly journals Performance of 'Ponkan' Mandarin on Seven Rootstocks in Southern Brazil

HortScience ◽  
2003 ◽  
Vol 38 (2) ◽  
pp. 176-178 ◽  
Author(s):  
Neusa M.C. Stenzel ◽  
Carmen S.V.J. Neves ◽  
José C. Gomes ◽  
Cristiane C. Medina
Keyword(s):  
Fruit Weight ◽  
Southern Brazil ◽  
Trifoliate Orange ◽  
Alternate Bearing ◽  
Rough Lemon ◽  
Volkamer Lemon ◽  
Canopy Volume ◽  
Significant Difference ◽  
Ponkan Mandarin ◽  
Cleopatra Mandarin

This study reports the performance (yield, tree size, and fruit quality) of 'Ponkan' mandarin (Citrus reticulata Blanco) on seven rootstocks, evaluated for 11 years under Southern Brazil conditions. Trees on C13 citrange had higher cumulative yield for seven harvests than those on trifoliate orange. Cleopatra mandarin, rough lemon, Rangpur lime, Sunki mandarin, and Volkamer lemon rootstocks maintained their values at an intermediate position and did not present any significant difference regarding C13 citrange, and trifoliate orange. Trees on C13 citrange and on trifoliate orange exhibited the lowest alternate bearing index. Cleopatra mandarin induced the greatest canopy volume, but it was not significantly different from Sunki mandarin and rough lemon. The smallest trees were those on Volkamer lemon and trifoliate orange. The highest yield efficiency came from trees on C13 citrange and the smallest on Cleopatra mandarin. Rootstocks did not significantly affect fruit weight.

scholarly journals Minigrafting of Shoots, Roots, Inverted Roots, and Somatic Embryos for Rescue of in vitro Citrus Regenerants

1999 ◽  
Vol 124 (2) ◽  
pp. 152-157 ◽  
Author(s):  
Fabio De Pasquale ◽  
Salvatore Giuffrida ◽  
Francesco Carimi
Keyword(s):  
Tissue Culture ◽  
Leaf Area ◽  
Somatic Embryos ◽  
Sweet Orange ◽  
Poncirus Trifoliata ◽  
Sour Orange ◽  
Cleopatra Mandarin ◽  
Troyer Citrange

Minigrafting was used for rescue of tissue culture regenerants of the following four species of Citrus: sour orange (C. aurantium L. `AA CNR 31'), sweet orange [C. sinensis (L.) Osb. `Valencia Late'], lemon [C. limon (L.) Burm. `Femminello Comune'] and mandarin (C. deliciosa Tenore `Tardivo di Ciaculli'). The grafting was carried out with different scion types including shoots, roots, inverted roots and somatic embryos. This material was obtained in vitro from embryogenic style-derived callus. Seedlings of open-pollinated sour orange (C. aurantium L.), Cleopatra mandarin (C. reshni Hort. ex Tan.) and `Troyer' citrange [C. sinensis Osb. × Poncirus trifoliata (L.) Raf.] were used as rootstocks. Minigrafting of shoots, roots, inverted roots and embryos regenerated in vitro allowed successful rescue of these four species. Percentages of successful minigrafts ranged from 100% (shoots) to 2.5% (inverted roots). The probability of successful graft unions increased with the age of the rootstock. The final mean canopy leaf area (120 days after grafting) ranged from 5.2 cm2 (`Tardivo di Ciaculli' mandarin grafted on 6-month-old Cleopatra mandarin) to 157.9 cm2 (`Valencia Late' sweet orange grafted on 18-month-old Cleopatra mandarin). In this work we examined some of the variables which influenced minigrafting and we determined the efficacy of this method for rescue of in vitro regenerants of Citrus. This method is also suggested as a technique to produce a high percentage of viable plants from in vitro regenerants difficult to root.

Sign in / Sign up

Export Citation Format

Share Document