Qualitative composition determination of the biologically active compounds in raw material of Centaurium erythraea Rafn. and Centaurium pulchellum (Sw.) Druce herb by liquid chromatographymass spectrometry method

Summary Eight species of Eleutherococcus genus grown at Rogów Arboretum collection were compared in respect of biologically active compounds accumulation, i.e. eleutheroside B and E, phenolic acids and sterols. For the determination of content of these compounds in underground organs and stem bark high performance liquid chromatography was applied. The highest content of the sum of eleutherosides B and E was observed in underground organs and stem bark of E. leucorrhizus (322.0 and 300.8 mg × 100 g-1, respectively) and E. nodiflorus (218.9 and 363.5 mg × 100g-1, respectively). In the raw materials from E. senticosus, the content of these compounds was significantly lower (177.4 and 159.3 mg × 100 g-1, respectively). E divaricatus and E. setchuenensis were characterized by the lowest accumulation of these compounds in underground organs whereas E. divaricatus, E. sessiliflorus and E. giraldii - in stem bark. Four phenolic acids were identified in the investigated species, namely: chlorogenic, rosmarinic, ferulic and caffeic acids. The main phenolic acid in the analyzed species was chlorogenic acid. The content of this compound in underground organs varied from 102.1 (E. henryi) to 958.7 mg × 100 g-1 (E. leucorrhizus) and in stem bark from 26.7 (E. giraldii) to 542.5 mg × 100 g-1 (E. setchuenensis). The content of identified sterol compounds (sitosterol 3-O-β-D-glucoside /eleutheroside A / campesterol, stigmasterol) was relatively low, but higher in underground organs in comparison with stem bark.

Download Full-text

Lonicera caerulea L. as a Source of Biologically Active Compounds for the Enrichment of Fermented Milk Product

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. ◽

10.2478/prolas-2021-0067 ◽

2021 ◽

Vol 75 (6) ◽

pp. 449-456

Author(s):

Dalija Segliņa ◽

Inta Krasnova ◽

Sanita Alsiņa

Keyword(s):

Raw Materials ◽

Fermented Milk ◽

Biologically Active Compounds ◽

Biologically Active ◽

Raw Material ◽

Future Research ◽

Milk Product ◽

Lonicera Caerulea ◽

Active Compounds ◽

Promising Source

Abstract Lonicera caerulea L., also known as honeysuckle berries, are rich in biologically active compounds, which makes them a valuable raw material for the development of functional foods. The objects of the research were three honeysuckle cultivars (‘Zoluška’, ‘Siņaja Ptica’, ‘Goluboje Vereteno’), four genotypes (No 2A; No 3; No 5; No 8) and kefir, enriched with various amounts (3–10%) of fruit puree. Physical and chemical analyses to determine the qualitative indicators of raw materials and products were performed. The degree of liking of kefir was assessed using sensory analysis: hedonic and line scales. The results obtained showed that among the cultivars the highest content of anthocyanins (1103.5 mg·100 g−1), total phenols (693.3 mg·100 g−1), flavonoids (753.9 mg·100 g−1), and tannins (1.6 mg·100 g−1), were found in ‘Siņaja Ptica’ berries, while among the genotypes, berries of No 8 had the highest concentration of the aforesaid compounds. Both samples also showed the highest antiradical activity: ABTS+• on average by 71.7% and DPPH− by 43.7%. According to the sensory evaluation, kefir with 10% honeysuckle puree was rated the highest. Honeysuckle berries could be used as a promising source of natural anti-oxidants in future research aimed at developing different new products that could meet consumer expectations.

Download Full-text

State and prospects of modern research in biotechnology of licens as a medicinal raw material

Bulletin of the State Nikitsky Botanical Gardens ◽

10.36305/0513-1634-2021-140-120-129 ◽

2021 ◽

pp. 120-129

Author(s):

E. F. Semyonova ◽

I. M. Teplitskaya ◽

M. A. Goncharov ◽

D. A. Goncharov

Keyword(s):

Usnic Acid ◽

Biologically Active Compounds ◽

Water Soluble ◽

Biologically Active ◽

Raw Material ◽

Active Compounds ◽

Alternaria Sp ◽

Ancient Times ◽

Methodological Approaches ◽

Original Experimental Data

Based on the content analysis of the sources of patent and scientific literature on the biotechnology of lichens as a medicinal raw material, systematized modern information is provided, as well as original experimental data regarding the cultivation of botanical species of lichens of various ecological and geographical groups under controlled conditions. Since ancient times, lichens havebeen used as medicines and are included in the pharmacopoeias of various countries. At present, methodological approaches have been developed for the cultivation of lichens (genera Usnea, Rhinocarpon, Umbilicara ) and their components: phycobiont (genera Nostoc, Chlorella ), mycobiont ( Petrusaria pertiza, Leydea parasema, Alternaria sp.). The main biologically active compounds (usnic acid, lichenin, cetrarin, water-soluble vitamins, calcium oxalate) and the pharmacological effects caused by them (antimicrobial, stimulating appetite, enveloping, anti-inflammatory, anti-burn, regenerating). Biotechnological approaches can be implemented for species cloning, reproduction, creation of a bank of cell cultures of lichens and their components in order to preserve economically valuable producers in an active state and develop technologies for obtaining biomass and pharmacologically valuable biologically active compounds.

Download Full-text