Niclosamide regulates the cell membrane properties in an intestinal barrier model

2013 ◽  
Vol 1 (Suppl. 1) ◽  
pp. A3.3
Author(s):  
Christine Schäfer
Keyword(s):  
Cell Membrane ◽  
Intestinal Barrier ◽  
Membrane Properties ◽  
Barrier Model

scholarly journals Quantitative Analysis of the Membrane Affinity of Local Anesthetics Using a Model Cell Membrane

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 579
Author(s):  
Wanjae Choi ◽  
Hyunil Ryu ◽  
Ahmed Fuwad ◽  
Seulmini Goh ◽  
Chaoge Zhou ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Local Anesthetics ◽  
Electrophysiological Study ◽  
Local Area ◽  
Membrane Properties ◽  
Dominant Factor ◽  
Free Standing ◽  
Membrane Affinity ◽  
Conformational Effects ◽  
Model Protein

Local anesthesia is a drug that penetrates the nerve cell membrane and binds to the voltage gate sodium channel, inhibiting the membrane potential and neurotransmission. It is mainly used in clinical uses to address the pain of surgical procedures in the local area. Local anesthetics (LAs), however, can be incorporated into the membrane, reducing the thermal stability of the membrane as well as altering membrane properties such as fluidity, permeability, and lipid packing order. The effects of LAs on the membrane are not yet fully understood, despite a number of previous studies. In particular, it is necessary to analyze which is the more dominant factor, the membrane affinity or the structural perturbation of the membrane. To analyze the effects of LAs on the cell membrane and compare the results with those from model membranes, morphological analysis and 50% inhibitory concentration (IC50) measurement of CCD-1064sk (fibroblast, human skin) membranes were carried out for lidocaine (LDC) and tetracaine (TTC), the most popular LAs in clinical use. Furthermore, the membrane affinity of the LAs was quantitatively analyzed using a colorimetric polydiacetylene assay, where the color shift represents their distribution in the membrane. Further, to confirm the membrane affinity and structural effects of the membranes, we performed an electrophysiological study using a model protein (gramicidin A, gA) and measured the channel lifetime of the model protein on the free-standing lipid bilayer according to the concentration of each LA. Our results show that when LAs interact with cell membranes, membrane affinity is a more dominant factor than steric or conformational effects of the membrane.

Modeling the Mechanics of Tethers Pulled From the Cochlear Outer Hair Cell Membrane

2008 ◽  
Vol 130 (3) ◽  
Author(s):  
Kristopher R. Schumacher ◽  
Aleksander S. Popel ◽  
Bahman Anvari ◽  
William E. Brownell ◽  
Alexander A. Spector
Keyword(s):  
Cell Membrane ◽  
Hair Cell ◽  
Outer Hair Cell ◽  
Lateral Wall ◽  
Outer Hair Cells ◽  
The Body ◽  
Computational Method ◽  
Membrane Properties ◽  
Bending Modulus ◽  
Sound Amplification

Cell membrane tethers are formed naturally (e.g., in leukocyte rolling) and experimentally to probe membrane properties. In cochlear outer hair cells, the plasma membrane is part of the trilayer lateral wall, where the membrane is attached to the cytoskeleton by a system of radial pillars. The mechanics of these cells is important to the sound amplification and frequency selectivity of the ear. We present a modeling study to simulate the membrane deflection, bending, and interaction with the cytoskeleton in the outer hair cell tether pulling experiment. In our analysis, three regions of the membrane are considered: the body of a cylindrical tether, the area where the membrane is attached and interacts with the cytoskeleton, and the transition region between the two. By using a computational method, we found the shape of the membrane in all three regions over a range of tether lengths and forces observed in experiments. We also analyze the effects of biophysical properties of the membrane, including the bending modulus and the forces of the membrane adhesion to the cytoskeleton. The model’s results provide a better understanding of the mechanics of tethers pulled from cell membranes.

Studies on cell membrane properties in food restricted rats

1991 ◽  
Vol 3 (4) ◽  
pp. 401-403 ◽  
Author(s):  
C. Pieri ◽  
M. Falasca ◽  
F. Moroni ◽  
F. Marcheselli ◽  
R. Recchioni
Keyword(s):  
Cell Membrane ◽  
Membrane Properties

Voltage-gated K+ channels at an early stage of chronic hypoxia-induced pulmonary hypertension in newborn piglets

2006 ◽  
Vol 291 (6) ◽  
pp. L1169-L1176 ◽  
Author(s):  
Candice D. Fike ◽  
Mark R. Kaplowitz ◽  
Yongmei Zhang ◽  
Jane A. Madden
Keyword(s):  
Pulmonary Hypertension ◽  
Smooth Muscle ◽  
Cell Membrane ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Chronic Hypoxia ◽  
Early Stage ◽  
Membrane Properties ◽  
Voltage Gated ◽  
Muscle Cell Membrane

Our purpose was to determine whether smooth muscle cell membrane properties are altered in small pulmonary arteries (SPA) of piglets at an early stage of pulmonary hypertension. Piglets were raised in either room air (control) or hypoxia for 3 days. A microelectrode technique was used to measure smooth muscle cell membrane potential ( Em) in cannulated, pressurized SPA (100- to 300-μm diameter). SPA responses to the voltage-gated K+ (KV) channel antagonist 4-aminopyridine (4-AP) and the KV1 family channel antagonist correolide were measured. Other SPA were used to assess amounts of KV1.2, KV1.5, and KV2.1 (immunoblot technique). Em was more positive in SPA of chronically hypoxic piglets than in SPA of comparable-age control piglets. The magnitude of constriction elicited by either 4-AP or correolide was diminished in SPA from hypoxic piglets. Abundances of KV1.2 were reduced, whereas abundances of both KV1.5 and KV2.1 were unaltered, in SPA from hypoxic piglets. At least partly because of reduced amounts of KV1.2, smooth muscle cell membrane properties are altered such that Em is depolarized and KV channel family function is impaired in SPA of piglets at an early stage of chronic hypoxia-induced pulmonary hypertension.

Effect of magnesium ions on red cell membrane properties

1990 ◽  
Vol 118 (3) ◽  
pp. 251-257 ◽  
Author(s):  
G. H. Beaven ◽  
J. Parmar ◽  
G. B. Nash ◽  
P. M. Bennett ◽  
W. B. Gratzer
Keyword(s):  
Cell Membrane ◽  
Membrane Properties ◽  
Red Cell ◽  
Magnesium Ions ◽  
Red Cell Membrane

Deformability and cell membrane properties of erythrocytes in Duchenne muscular dystrophy

1983 ◽  
Vol 130 (2) ◽  
pp. 139-147 ◽  
Author(s):  
Telford Conlon ◽  
Philip S. Lingard ◽  
John K. Tomkins
Keyword(s):  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Cell Membrane ◽  
Membrane Properties

scholarly journals Correction: Alterations of Red Cell Membrane Properties in Nneuroacanthocytosis

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
Author(s):  
Claudia Siegl ◽  
Patricia Hamminger ◽  
Herbert Jank ◽  
Uwe Ahting ◽  
Benedikt Bader ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Properties ◽  
Red Cell ◽  
Red Cell Membrane
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