scholarly journals Effect of tanshinone on the levels of nitric oxide synthase and acetylcholinesterase in the brain of alzheimer’s disease rat model

2008 ◽  
Vol 31 (5) ◽  
pp. 248 ◽  
Author(s):  
You Yin ◽  
Liuqing Huang ◽  
Yan Liu ◽  
Shaqi Huang ◽  
JianHua Zhuang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Treatment Group ◽  
Nitric Oxide Synthase ◽  
Rat Model ◽  
Therapeutic Potential ◽  
Control Group ◽  
Group Model ◽  
Model Group ◽  
Oxide Synthase ◽  
The Brain

Purpose: To determine the influence of tanshinone on the levels of nitric oxide synthase (NOS) and acetylcholinesterase (AChE) in the brain of an Altzheimer Disease (AD) rat model and on its potential therapeutic mechanism. Methods: 100 Male Sprague Dawley rats were divided into three groups: control group, model group and tanshinone treatment group. 10µg A?1-42 was injected bilaterally into the dorsal lateral region of the dentate gyrus in the hippocampus of rats in the model and tanshinone treatment groups to prepare the AD models. 24h after modeling, tanshinone, 50mg/kg, was administered by gastric perfusion to rats in the tanshinone treatment group. Later, immunohistochemical assay and Western blot analysis were used to detect expression of neuronal NOS (nNOS) and inducible NOS (iNOS) in the rat hippocampus. Activity of AChE in each subregion (CA1~CA4) of rats’ hippocampus was determined by a histochemical technique. Results: Expression of nNOS in the model group was down-regulated whereas iNOS was up-regulated. After A?1-42 injection, the number of AChE positive fibers in each subregion (CA1~CA4) of the hippocampus was decreased compared with controls. With tanshinone administration, the changes were improved to varying degrees. Conclusion: Tanshinone modulates AChE and NOS proteins concentrations in the hippocampus of AD rats. This may have therapeutic potential in AD rats.

Localization of the cGMP-dependent protein kinases in relation to nitric oxide synthase in the brain

1999 ◽  
Vol 17 (1) ◽  
pp. 45-55 ◽  
Author(s):  
A.E.-D El-Husseini ◽  
J Williams ◽  
P.B Reiner ◽  
S Pelech ◽  
S.R Vincent
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Protein Kinases ◽  
Dependent Protein ◽  
Oxide Synthase ◽  
The Brain

Impaired pulmonary artery contractile responses in a rat model of microgravity: role of nitric oxide

2002 ◽  
Vol 92 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Daniel Nyhan ◽  
Soonyul Kim ◽  
Stacey Dunbar ◽  
Dechun Li ◽  
Artin Shoukas ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Pulmonary Artery ◽  
Lung Tissue ◽  
Rat Model ◽  
Orthostatic Intolerance ◽  
Volume Distribution ◽  
Contractile Responses ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Vascular contractile hyporesponsiveness is an important mechanism underlying orthostatic intolerance after microgravity. Baroreceptor reflexes can modulate both pulmonary resistance and capacitance function and thus cardiac output. We hypothesized, therefore, that pulmonary vasoreactivity is impaired in the hindlimb-unweighted (HLU) rat model of microgravity. Pulmonary artery (PA) contractile responses to phenylephrine (PE) and U-46619 (U4) were significantly decreased in the PAs from HLU vs. control (C) animals. N G-nitro-l-arginine methyl ester (10−5 M) enhanced the contractile responses in the PA rings from both C and HLU animals and completely abolished the differential responses to PE and U4 in HLU vs. C animals. Vasorelaxant responses to ACh were significantly enhanced in PA rings from HLU rats compared with C. Moreover, vasorelaxant responses to sodium nitroprusside were also significantly enhanced. Endothelial nitric oxide synthase (eNOS) and soluble guanlyl cyclase expression were significantly enhanced in PA and lung tissue from HLU rats. In marked contrast, the expression of inducible nitric oxide synthase was unchanged in lung tissue. These data support the hypothesis that vascular contractile responsiveness is attenuated in PAs from HLU rats and that this hyporesponsiveness is due at least in part to increased nitric oxide synthase activity resulting from enhanced eNOS expression. These findings may have important implications for blood volume distribution and attenuated stroke volume responses to orthostatic stress after microgravity exposure.

Inhibition of Inducible Nitric Oxide Synthase Reduces Bacterial Translocation in a Rat Model of Acute Pancreatitis

Pancreas ◽  
2001 ◽  
Vol 23 (3) ◽  
pp. 296-301 ◽  
Author(s):  
Ismail Simsek ◽  
Mehmet Refik ◽  
Mehmet Yasar ◽  
Mustafa Ozyurt ◽  
Ugur Saglamkaya ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Acute Pancreatitis ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Bacterial Translocation ◽  
Rat Model ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Changes in Nitric Oxide Synthase-Containing Neurons in the Brain of Thiamine-Deficient Mice.

2000 ◽  
Vol 33 (2) ◽  
pp. 67-72 ◽  
Author(s):  
Hiroko Matsushita ◽  
Yoshihiro Takeuchi ◽  
Kitaro Kosaka ◽  
Shinji Fushiki ◽  
Mitsuhiro Kawata ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
The Brain ◽  
Deficient Mice

scholarly journals Therapeutic Potential of Nitric Oxide Synthase Gene Manipulation

Circulation ◽  
2001 ◽  
Vol 103 (22) ◽  
pp. 2760-2765 ◽  
Author(s):  
Heiko E. von der Leyen ◽  
Victor J. Dzau
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Therapeutic Potential ◽  
Gene Manipulation ◽  
Oxide Synthase

scholarly journals Preventive Effect of Lactobacillus Plantarum CQPC10 on Activated Carbon Induced Constipation in Institute of Cancer Research (ICR) Mice

2018 ◽  
Vol 8 (9) ◽  
pp. 1498 ◽  
Author(s):  
Jing Zhang ◽  
Xianrong Zhou ◽  
Benshou Chen ◽  
Xingyao Long ◽  
Jianfei Mu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Activated Carbon ◽  
Nitric Oxide Synthase ◽  
Lactobacillus Plantarum ◽  
Quantitative Polymerase Chain Reaction ◽  
Inhibitory Effect ◽  
Lactobacillus Bulgaricus ◽  
Control Group ◽  
Expression Levels ◽  
Oxide Synthase

Chinese Paocai is a traditional fermented food containing an abundance of beneficial microorganisms. In this study, the microorganisms in Szechwan Paocai were isolated and identified, and a strain of lactic acid bacteria (Lactobacillus plantarum CQPC10, LP-CQPC10) was found to exert an inhibitory effect on constipation. Microorganisms were isolated and identified via 16S rDNA. Activated carbon was used to induce constipation in a mouse model and the inhibitory effect of LP-CQPC10 on this induced constipation was investigated via both pathological sections and qPCR (quantitative polymerase chain reaction). A strain of Lactobacillus plantarum was identified and named LP-CQPC10. The obtained results showed that, as compared to the control group, LP-CQPC10 significantly inhibited the amount, weight, and water content of faeces. The defecation time of the first tarry stool was significantly shorter in LP-CQPC10 groups than in the control group. The activated carbon progradation rate was significantly higher when compared to the control group and the effectiveness was improved. LP-CQPC10 increased the serum levels of MTL (motilin), Gas (gastrin), ET (endothelin), AchE (acetylcholinesterase), SP (substance P), and VIP (vasoactive intestinal peptide), while decreasing the SS (somatostatin) level. Furthermore, it improved the GSH (glutathione) level and decreased the MPO (myeloperoxidase), MDA (malondialdehyde), and NO (nitric oxide) levels. The results of qPCR indicated that LP-CQPC10 significantly up-regulated the mRNA expression levels of c-Kit, SCF (stem cell factor), GDNF (glial cell-derived neurotrophic factor), eNOS (endothelial nitric oxide synthase), nNOS (neuronal nitric oxide synthase), and AQP3 (aquaporin-3), while down-regulating the expression levels of TRPV1 (transient receptor potential cation channel subfamily V member 1), iNOS (inducible nitric oxide synthase), and AQP9 (aquaporin-9). LP-CQPC10 showed a good inhibitory effect on experimentally induced constipation, and the obtained effectiveness is superior to that of Lactobacillus bulgaricus, indicating the better probiotic potential of this strain.

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