Działanie olejku ylangowego (Cananga oil) wobec grzybów drożdżopodobnych

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia
Keyword(s):  
Essential Oil ◽  
Growth Control ◽  
The Philippines ◽  
Geranyl Acetate ◽  
Low Concentrations ◽  
Acetate Methyl ◽  
Medicinal Properties ◽  
Therapeutic Properties ◽  
Reference Strains ◽  
Cananga Odorata

Introduction. Several herbs produce substances with medicinal properties. They show antioxidant, antiinflammatory and antimicrobial activity. Cananga odorata Hook (Annonaceae family) is found in the Philippines, Madagascar island, Sumatra, in Jemen and Australia. It is fast growing, evergreen tree, that reaches up to 20 m. Essential oil produced of flowers is used in medicine. The chemical composition of the Cananga oil is as follows: geraniol, linalool, methyl salicylate, α-terpineol, eugenol, α- and β-caryophyllene, farnesen, δ-cadinene, γ-kadinene, geranyl acetate, methyl p-cresyl ether, p-cresol, neridol, α-pinene, carbohydrates, saponins, tanins, flavonoids, amino acids and coumarins. It possess different therapeutic properties. Aim. The aim of the study was evaluation of antifungal activity of Cananga oil. Material and methods. The strains of fungi were isolated from oral cavity. They were from the following genera: Candida albicans (10 strains), C. glabrata (4), C. guilliermondii (2), C. kefyr (2), C. krusei (4), C. lusitaniae (2), C. parapsilosis (3), C. tropicalis (5) and C. utilis (2). Furthermore 9 reference strains C. albicans ATCC 10231, C. glabrata ATCC 66032, C. guilliermondii ATCC 6260, C. kefyr ATCC 4130, C. krusei ATCC 14249, C. lusitaniae ATCC 34499, C. parapsilosis ATCC 22019, C. tropicalis ATCC 750 and C. utilis ATCC 9958 were tested. The sensitivity (MIC) of the yeast to Cananga oil (Semifarm) was determined by method of plate dilution technique in Sabouraud’s agar. First the essential oil was dissolved in dimethyl sulfoxide (DMSO, Serva) and then in sterile distilled water. The oil concentrations were: 15.0, 10.0, 7.5, 5.0, 2.5 and 1.2 mg/ml. The inoculum containing 105 microorganisms per drop (CFU) was applied with Steers replicator on agar with or without oil (strains growth control). Incubation of the agar plates was carried out under aerobic conditions at 37°C for 12-48 hrs. The MIC was defined as the lowest concentration of the Cananga oil inhibited the growth of the tested strains of yeast-like fungi. Results. The results show, that the growth of 97% of the tested strains of yeast-like fungi was inhibited in the concentration range 1.0-10.0 mg/ml. The strains of C. albicans and C. glabrata were susceptible in range 2.5-5.0 mg/ml. The oil shoved activity against C. guilliermondii and C. utilis strains at a concentration of 5.0 mg/ml. Only 1 strain of C. parapsilosis and 1 strain of C. tropicalis were susceptible at 1.2 mg/ml. However, the oil shoved the lowest activity against C. krusei strains. The MIC values of Cananga oil ranged from 10.0 to 15.0 mg/ml. Conclusions. Most of the estimated strains from Candida genus were susceptible to low concentration of Cananga oil. The oil in low concentrations inhibited the grows of C. albicans strains. The C. krusei strains were the least sensitive to Cananga oil.

Wrażliwość grzybów drożdżopodobnych na olejek majerankowy (Oleum Majoranae)

2019 ◽  
Vol 20 (4) ◽  
Author(s):  
Anna Kędzia ◽  
Andrzej W. Kędzia
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Growth Control ◽  
Dilution Method ◽  
Oxygenated Monoterpenes ◽  
Sesquiterpene Hydrocarbons ◽  
Visible Growth ◽  
Therapeutic Properties ◽  
Antioxidant And Antimicrobial Activity ◽  
Reference Strains

Introduction. Majoram possesses a number of therapeutic properties. Essential oil contain, following components: oxygenated monoterpenes (α-and β-pinene, camphene, α-phellandrene, α-, β-, and δ-terpinene, cymene), oxygenated monoterpenes (1,8-cineoe, cis-sabinene hydrate, linalool, terpinene-4-ol, α-tepineol, linalyl acetate), sesquiterpene hydrocarbons (cubebene, β-longpinene, β-caryophyllene, α-humulene, β-farnezene) and oxygenated sesquiterpenes (caryophyllene oxide). Majoran essential oil posses anti-inflammatory, antioxidant and antimicrobial activity. Aim. The aim of this work was investigate the susceptibility of yeastlike fungi to majoram oil. Material and methods. Yeastlike fungi were isolated from oral cavity of patients with candidosis. The strains of fungi belonging to the following genus: Candida albicans (12 strains), C. glabrata (2), C. guilliermondii (1), C. humicola (1), C. kefyr (2), C. krusei (4), C. lusitaniae (1), C. parapsilosis (4), C. tropicalis (5) and C. utilis (1) and 9 reference strains were tested. Investigation was carried out using the plate dilution method in Sabouraud’s agar. Inoculum contain 105 microorganisms per spot was seeded with Steers replicator upon surface of agar with and without essential oil (strain growth control). The dates of concentrations of majoran oil (Semifarm) was 2.0, 1.0, 0.5, 0.25 and 0.12 mg/ml. Inoculated plates were incubated in aerobic conditions at 37°C for 24-48 hrs. The MIC was defined as the lowest concentration of majoram oil inhibiting the visible growth of yeastlike fungi. Results. The results of the dates indicated that the strains from genus of Candida was susceptible to oil in concentration from 1.0 to ≥ 2.0 mg/ml. The growth of the strain Candida albicans, C. guilliermondii and C. humicola were inhibited in concentration 1.0 mg/ml. The strains of C. glabrata, C. kefyr, C. krusei, C. parapsilosis and C. tropicalis were less sensitive (MIC 1.0-≥ 2.0 mg/ml). However the strains of C. lusitaniae and C. utilis were the lowest sensitive. The growth of this yeastlike fungi was inhibited by concentrations ≥ 2.0 mg/ml. Conclusions. The tested yeastlike fungi were variability susceptible to majoram oil. The oil was the most active towards C. albicans, C. guilliermondii and C. humicola strains. Strains of C. lusitaniae and C. utilis were the lowest sensitive on majoram oil.

Przeciwgrzybicza aktywność olejku melisowego (Oleum Melissae)

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Anna Kędzia ◽  
Andrzej W. Kędzia
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Candida Glabrata ◽  
Candida Utilis ◽  
Growth Control ◽  
Aerobic Condition ◽  
Geranyl Acetate ◽  
Candida Lusitaniae ◽  
Antibacterial And Antifungal ◽  
Reference Strains

Introduction. A lot of plant produced essential oils, which are applied in prophylaxis and therapy of medicine. Melissa was known and used in I century BC. It produced the oil, which possess following properties: antiarteriosclerotic, anticancer, sedative, antidepression, antimigraine, antiasthmatic, antirheumatic and antioxidant. Its contain: geraniol, β-caryophyllene, geranial, thymol, neral, geranyl acetate, linalol, cytronellol, citronellal and α-humulen. It exhibited antibacterial and antifungal activity. Aim. The aim of this study was to indicate of susceptibility of yeastlike fungi to melissae essential oil. Material and methods. The strains of fungi were isolated from oral cavity from patients with candidosis. A total 23 strains of yeastlike fungi from genus of Candida albicans (22 strains), C. glabrata (5), C. guilliermondii (2), C. humicola (2), C. kefyr (3), C. krusei (5), C. lusitaniae (2), C. parapsilosis (5), C. tropicalis (6), C. utilis (1) and 9 reference strains were tested. Investigated was carried out using plate dilution technique in Sabouraud’s agar. The melissae oil (Semifarm) was dissolved in DMSO and then in aseptic distilled water. Inoculum contain 105 CFU per spot was transferred with Steers replicator upon the agar with and without essential oil (strains growth control). The concentrations of melissae oil were: 2.0, 1.0, 0.5, 0.25 and 0.12 mg/ml. Incubation was performed in aerobic conditions in temp. 37°C. Incubation of agar plates were performed in aerobic condition at temp. 37°C, at 24-48 hours. Minimum inhibitory concentration (MIC) was interpreted as the lowest concentrations of melissa oil which inhibited the growth of yeastlike fungi. Results. The dates indicated that the strains of fungi was susceptible to oil in concentrations 0.25-2.0 mg/ml. The 19 (86%) of strains from genus Candida albicans was inhibited in concentrations 0.25-0.5 mg/ml. On the same values of MIC’s were susceptible the strains of Candida albicans, Candida glabrata and Candida humicola (MIC 0.5 mg/ml). The fungi from genus of Candida kefyr, Candida krusei, Candida lusitaniae and Candida tropicalis were less sensitive. The growth of this yeastlike fungi was inhibited by concentrations of melissae oil in range 0.5-2.0 mg/ml. The oil was the lowest active towards genus Candida lusitaniae and Candida utilis. The MIC for these strains was from 1.0 to 2.0 mg/ml. From all tested genus Candida strains 11 (21%) of them was susceptible to melissa oil in range 2.0 mg/ml. Conclusions. Melissa oil was the most active towards strains of Candida albicans, Candida glabrata and Candida humicola. The lowest sensitive to oil were the strains from genus Candida lusitaniae and Candida utilis. The melissa oil characterized a high activity towards all tested strains of yeastlike fungi from genus of Candida.

Wrażliwość bakterii beztlenowych na australijski olejek mirtowy (Backhousia citriodora F. Muell.)

2018 ◽  
Vol 19 (3) ◽  
Author(s):  
Anna Kędzia ◽  
Andrzej W. Kędzia ◽  
Henry Ostrowski-Meissner ◽  
Joanna Wiśniewska
Keyword(s):  
Essential Oil ◽  
Anaerobic Bacteria ◽  
Growth Control ◽  
Volatile Oil ◽  
Gram Positive ◽  
Gram Negative ◽  
The Bible ◽  
Ancient Times ◽  
Lung Infections ◽  
Reference Strains

Introduction. Herbs have been known and utilize since ancient Times in China, Europe and America. The ancient Egiptians used the myrtle to treat fever and infections. The plant have been mentioned in the Bible six times. Dioscorides recommended myrtle oil on patients with blader and lung infections. The herb and essential oil were used in Ayuveric treating infections respiratory tract and neuralgic. Backhousia citriodora F. Muell. belonging to the genus Backhousia and family Myrtaceae. It grown to 20-30 m high, on plantations in Australia, in subtropical rainforests of central and south-eastern Queensland. The leaves of the tree Backhousia citriodora produces an essential oil. It is obtained via hydro distillation. Lemon myrtle oil is known for its characteristic lemon flavor, related with presence of citral. Research indicated, that constituents of oil showed antibacterial activity against aerobic bacteria. There are no information about its activity towards anaerobic bacteria. Aim. The aim of the study was to determine activity of myrtle oil against anaerobic bacteria isolated from patients. Material and methods. The anaerobic bacteria were isolated from various infections of oral cavity. A total 135 strains isolated from patients and 7 reference strains were tested. The suscibility anaerobes to myrtle oil was carried out using plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. Inoculum containing 106 in CFU per spot was seeded with Steers replicator upon the surface of agar with myrtle oil and without oil (strains growth control). Incubation the plates was performed in anaerobic jars (10% C02 , 10% H2 , 80% N2 , palladic catalyst and anaerobic indicator) at 37°C for 48 hrs. The MIC was defined as the lowest concentrations of the myrtle oil that completely inhibited growth the tested anaerobic bacteria. Results. The results indicated that the myrtle oil was active against the 44 (50%) from all the tested strains of anaerobes to the concentrations from ranges < 0.12-1.0 mg/ml. The rods from genus of Prevotella oralis and Prevotella oris were sensitive on concentrations < 0.12 mg/ml. The oil was less active against the Gram-negative anaerobes from genus of Prevotella buccalis, Prevotella levii, Prevotella loescheii, Bacteroides uniformis and Parabacteroides distasonis (MIC > 4.0 mg/ml). The Clostridial strains were very sensitive. The growth of this strains was inhibited by concentrations of < 0.12 mg/ml. The volatile oil did appeared to be equally effective against both Gram-negative and Gram-positive anaerobic rods. The growth of 50 and 47% of this bacteria was inhibited in ranges from < 0.12 to 1.0 mg/ml. The tested Gram-negative cocci from genus Veillonella parvula were less sensitive (MIC 1.0-> 4.0 mg/ml). The strains of Gram-positive cocci were the least sensitive. Its growth was inhibited by concentrations 2.0-> 4.0 mg/ml. Conclusions. The myrtle oil was more active against anaerobic bacteria from genus Prevotella oralis, Prevotella oris and strains from genera of Clostridium. The strains from genus Prevotella buccalis, Prevotella levii, Prevotella loescheii, Bacteroides uniformis and Parabacteroides distasonis were the lowest sensitive. The oil was equally effective against Gram-negative and Gram-positive anaerobic bacteria.

Oddziaływanie na bakterie beztlenowe olejku jałowcowego (Oleum Juniperi)

2018 ◽  
Vol 19 (4) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia
Keyword(s):  
Essential Oil ◽  
Folk Medicine ◽  
Anaerobic Bacteria ◽  
Growth Control ◽  
Antimicrobial Properties ◽  
Gram Positive ◽  
Gram Negative ◽  
Propionibacterium Granulosum ◽  
Reference Strains ◽  
Gram Positive Cocci

Introduction. Juniper berries have been used in ancient in folk medicine for treatment infections, as a spice for meat, and preparation of gin and raki. Juniper (Juniperus communis L.) belongs to the Cupressaceae family. It is an evergreen tree or shrub and achieve from 2 to 10 m high. Its berries produced essential oil, which contain α-pinene, camphene, limonene, cadinene, terpinen-4-ol, myrcene, β-pinene, sabinene, flavonoids, resins, organic acids, and tannins. The oil is used for treatment various diseases. It demonstrate antimicrobial properties. Aim. The aim of the study was to investigate the susceptibility of anaerobic bacteria of oral cavity to juniper oil. Material and methods. The 56 of anaerobic bacteria isolated from patients, in it Gram-negative rods (31 strains), Gram-positive cocci (13) Gram-positive rods (12) and 8 reference strains were tested. Investigation was carried out using the plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. Inoculum containing 106 CFU per spot was seeded with Steers replicator upon the agar with oil or without the oil (strains growth control). Concentration of dated oil were: 2,0, 5,0, 7,5, 10,0, 15,0 and 20,0 mg/ml. The plate was incubated in anaerobic condition in anaerobic jar in 37°C for 48 hrs. The MIC was interpreted as the lowest concentration of juniper oil inhibiting the growth of tested bacteria. Results and discussion. The results of the experiments indicated, that rods from Gram-negative anaerobes, the strains belonging to the genera of Prevotella and Porphyromonas, were the most susceptible to essential oil. The MIC’s for the strains be within from 5,0 to 10,0 mg/ml. The lowest sensitive were the rods from genera Bacteroides, Parabacteroides and Tannerella (MIC > 20,0 mg/ml). From among Gram-positive cocci the most sensitive to oil were the strains from genus Finegoldia magna. The oil was active towards half of the strains in concentration < 2,5 mg/ml. The tested Gram-positive rods were the less sensitive (MIC 5,0-> 20,0 mg/ml). From this group, the strains Actinomyces viscosus demonstrated the sensitivity on 5,0 to 7,5 mg/ml. The most resistance were the rods Actinomyces odontolyticus and Propionibacterium granulosum (MIC > 20,0 mg/ml). Conclusions. The lowest susceptible to juniper oil from Gram-negative bacteria were genus of Bacteroides, Parabacteroides and Tannerella. The oil was the most active against strains of Prevotella and Porphyromonas. The cocci were the more susceptible than Gram-positive rods. The Gram-positive anaerobes showed high susceptibility to juniperus oil than Gram-negative rods.

Aktywność olejku anyżowego (Oleum Anisi) wobec grzybów drożdżopodobnych

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia
Keyword(s):  
Essential Oil ◽  
Steam Distillation ◽  
Inhibitory Concentration ◽  
Growth Control ◽  
Annual Plant ◽  
Antibacterial And Antifungal Activity ◽  
Dilution Technique ◽  
Antibacterial And Antifungal ◽  
Slow Growing ◽  
Reference Strains

Introduction. Anise (Pimpinella anisum L.) is a slow growing annual plant from Apiaceae family of height that ranges from 30-50 cm. Anise fruits and produced essential oil are flavoring of foods, some medicines, toothpastes and mouthwashes. The essential oil is obtained by steam distillation from the nature fruits. Its main constituents are anetholes. Anise essential oil exhibited many biological and therapeutical activities. It has antibacterial and antifungal activity. Aim. The aim of this dates was to investigate the susceptibility of yeastlike fungi on anise oil. Material and methods. The sensitivity to anise oil of 74 strains yeastlike fungi and 9 reference strains were tested. The microorganism was from the following genera: Candida albicans (29 strains), C. glabrata (8), C. guilliermondii (2) C. humicola (2), C. kefyr (5), C. krusei (7), C. lusitaniae (2), C. parapsilosis (8), C. tropicalis (9) and C. utilis (2). Date was carried out using the plate dilution technique in Sabouraud’s agar. The concentrations of tested anise oil was: 2.0, 1.0, 0.50, 0.25, 0.12 and 0.06 mg/ml. The inoculum containing 105 CFU/spot was transfer with Steers replicator upon the surface of agar with and without tested oil (strain growth control). Incubation of agar plates were conducted in aerobic conditions in 37°C for 24-48 hrs. Minimum inhibitory concentration (MIC) was interpreted as the lowest concentrations of anise oil which inhibited the growth of strains of yeastlike fungi. Results. The results indicated that the strains of dates yeastlike fungi were susceptible to oil in concentrations from range 0.12-0.50 mg/ml. The most susceptible to anise oil were the strains from genera of C. guilliermondii, C. utilis (MIC 0.12-0.25 mg/ml) and C. lusitaniae (MIC = 0.25 mg/ml). The strains of C. albicans, which was isolated frequently, were susceptible in ranges 0.12-0.50 mg/ml. The oil was very active towards strains C. parapsilosis. Growth 87% of this strains was inhibited by concentrations 0.12-0.25 mg/ml. Moreover oil exhibited activity towards under half (55%) strains of C. tropicalis in concentration 0.12 mg/ml. The fungus from genus C. humicola were the lowest sensitive to anise oil (MIC = 0.5 mg/ml). Conclusions. Anise oil showed activity towards all tested yeastlike fungi. The most susceptible to oil characterized the strains from genera C. guilliermondii, C. utilis and C. lusitaniae. Oil was less active towards C. humicola strains.

scholarly journals STUDY OF THE CHEMICAL COMPOSITION IN LEAVES AND OIL OF Cananga odorata (Lam.) IN BEN TRE PROVINCE

2020 ◽  
Vol 1 (40) ◽  
pp. 92-102
Author(s):  
Chau Nguyen Minh Doan ◽  
Tin Thanh Nguyen
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Hexane Extract ◽  
Geranyl Acetate ◽  
Crystalline Solid ◽  
Water Volume ◽  
Fresh Weight ◽  
Benzyl Benzoate ◽  
Physical And Chemical ◽  
Cananga Odorata

Investigation of the chemical composition of Cananga odorata(Lam.) leaves and essential oil in Ben Tre Province was conducted with steamenticing distillation method, extraction nhexane and Soxhlet with the solvents of nhexane, dietyl ether respectively. The results have determined the distillation conditions and the content of Cananga odorata (Lam.) essential oil by fresh weight and water volume is: 1: 3 (gram / ml)with distillation time: 3 to 4 hours. The content essential oil of Cananga odorata (Lam.) flower obtained in dry weight is 1.10% -1.26% and in fresh weight is 0.56% - 0.65%. The results of the study identified some physical and chemical indexes: the essential oil of Cananga odorata (Lam.) flower in Ben Tre is yellow, clear, has a stronger aroma and is lighter than water; Physical and chemical indices of the essential oil of Cananga odorata(Lam.) flower measured in three different lands of Ben Tre and collected during the same period showed: The density changed from 0.92 - 0.95; Refractive index varies from 1.4952 - 1.4991; The acid index varies from 0.13 to 0.38; The ester index ranged from 37.55 to 38.11; The saponification index ranged from 37.67 to 38.49. Mass SpectrometryMS method has been identified that the main chemical composition the essential oil of Cananga odorata (Lam.) flower in three regions are consistent with the results of the authors who have studied theessential oil of Cananga odorata (Lam.) flower in the North and include the following components: L-linalol, geraniol, geranyl axetat, benzyl benzoat, anđehit benzoic, etyl benzoat, D-germacren,... In which, the main ingredents of Cananga odorata (Lam.) flower in n-hexane extract are: benzyl benzoate, D-germacren,L-linalol, b-caryophyllen; The main components when make in dietyl ether are: benzyl benzoate, geranyl acetate, anisyl acetone, D-germacren; The main ingredients of Cananga odorata (Lam.) leaf inn-hexane extract include: L-linalol, transgeraniol, geranyl acetate,...From n-hexane solvent extract, we isolated, purified and determined the structure of the crystalline solid is benzyl benzoate.

Ocena aktywności olejku imbirowego (Oleum Zingiberis) wobec bakterii beztlenowych

2018 ◽  
Vol 19 (2) ◽  
Author(s):  
Anna Kędzia ◽  
Andrzej W. Kędzia ◽  
Joanna Wiśniewska ◽  
Marek Ciecierski
Keyword(s):  
Anaerobic Bacteria ◽  
Growth Control ◽  
Zingiber Officinale ◽  
Volatile Oil ◽  
Geranyl Acetate ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Reference Strains ◽  
Gram Positive Cocci

Introduction. Zingiber officinale from family Zingiberaceae is known as ginger. Its common names are African Ginger, Cocchin Ginger, Jamaican Ginger, Black Ginger, Gan jiang, Ingwer, Gegibre and Rice Ginger. It was used in traditional Chinese and Ayurvedic medicine to treat headaches, nausea and colds. In Mexican medicine have been used to treat gastrointestinal complaints. It is one of the frequently used spices in many countries of the world. It can be consumed as a fresh or dried to prepared tea, soft drinks and bread. The plant grown to 1 m high. The rhizome contain volatile oil. The major components of ginger are mono- and sesquiterpens, in it zingiberene and zingiberol. Futhermore oil contain borneol, cyneole, citral, camphene, β-phellandrene, zingerone, shogaol, geranyl acetate, geraniol, curcumene, terpineol, limonene, linalool, α-farnesene, neral and 6-gingerol. Research carried out on ginger indicated, that components to have antiinflammatory, antiplateled aggregation, antioxidant, antidiabetic, cholesterol-lowering, blood pressure-lowering and anticancer properties. Aim. The goal of this dates was to test the antimicrobial activity of ginger oil against anaerobes. Material and methods. The anaerobic bacterial strains were isolated from oral cavity. A total 53 strains isolated from patients and 6 reference strains were examined. The members of following genera were tested: Porphyromonas (4 strains), Prevotella (9), Bacteroides (8), Parabacteroides (1), Tannerella (2), Fusobacterium (7) and after 11 strains of Gram-positive cocci and Gram-positive rods and 6 reference strains from genus: Bacteroides fragilis ATCC 25285, Porphyromonas asaccharolytica ATCC 29743, Fusobacterium nucleatum ATCC 25586, Finegoldia magna ATCC 29328, Peptostreptococcus anaerobius ATCC 27337 and Propionibacterium acnes ATCC 11827. Susceptibility (MIC) was determined by the two-fold dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. The inoculum containing 106 CFU/per spot was seeded with Steers replicator upon the surface of agar with ginger oil (Semifarm, Gdańsk) or without the oil (the strains growth control). Concentrations of oil used were 20.0, 10.0, 7.5, 5.0, 2.5 and 1.2 mg/ml. Incubation the plates was performed in anaerobic conditions in anaerobic jar, at 37°C for 48 hrs. The MIC was defined as the lowest concentrations of ginger oil that completely inhibited the growth of tested anaerobes. Results. The results showed, that the most susceptible from Gram-anaerobic bacteria to ginger oil in ranges ≤ 1.26-5.0 mg/ml were the strains from genus of Tannerella forsythia and Bacteroides uniformis. The others of Gram-negative rods were susceptible to oil in ranges 10.0-≥ 20.0 mg/ml. The strains belonging to the genus of Prevotella bivia, Prevotella buccalis and Parabacteroides distasonis were the lowest sensitive to tested oil (MIC ≥ 20.0 mg/ml). The ginger oil was very active against Gram-positive cocci. MIC’s for all the tested strains were to the concentrations from 5.0 to 10.0 mg/ml. The oil characterized similarly of activity in case Gram-positive rods. The date showed, that 82% this strains were susceptible to concentration – 10.0 mg/ml. Conclusions. The results indicated that the ginger oil showed antibacterial activity against all tested anaerobic bacteria. The more susceptible to oil were the Gram-positive cocci and rods then Gram-negative anaerobic bacteria.

Skuteczność działania in vitro olejku melisowego (Oleum Melissae) na bakterie beztlenowe

2019 ◽  
Vol 20 (4) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia
Keyword(s):  
Essential Oil ◽  
Anaerobic Bacteria ◽  
Growth Control ◽  
Geranyl Acetate ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
High Antibacterial Activity

Introduction. Melissa officinalis L. is a member of family Lamiaceae. The plant a widely cultivated in many countries of Asia (Iran, Turkiestan), North America, Europe and Poland. It growth to 1 m high. Leaves are green with characteristic smell of lemon and flowers are white or pink. The plant produce of essential oil which the components are: neral, geraniol, geranial, β-caryophyllene, tymol, linalol, citronellol, cytronellal, geranyl acetate, α-humulene, germacrane D, n-eikosane, didydrocitronellolacetate, 5-cedranone, β-ocimene Z and β-ocimene E. The oil is used in therapy. It showed antiinflammatory and antymicrobial activity towards bacteria, fungi, viruses and insects. Aim. The aim of this study was to evaluate the activity of melissa oil against anaerobic bacteria. Material and methods. The bacterial strains were isolated from oral cavity. A total 32 strains anaerobes and 8 standards strains were investigated. The melissa oil (Semifarm) was dissolved in DMSO and distilled water to obtained a final concentrations of 2.0, 1.0, 0.5, 0.25, 0.12 and 0.06 mg/ml. The inoculums containing 105 CFU/spot was seeded with Steers replicator upon the surface of agar with or without essential oil (bacterial strains growth control). Incubation was performed in anaerobic conditions in anaerobic jar, in 37°C for 48 hrs. The MIC was defined as the lowest concentrations of melissa oil inhibiting the growth of the tested anaerobes. Results. The results showed, that the melissa oil presented high antibacterial activity against all tested anaerobes. The most susceptible from Gram-positive bacteria were the cocci from the genus of Finegoldia magna, Micromonas micros and Peptostreptococcus anaerobius and Gram-positive rods Actinomyces odontolyticus and Bifidobacterium bivia (MIC < 0.06 mg/ml). The 92% of Gram-positive bacteria was inhibited in concentrations < 0.06-0.25 mg/ml. From Gram-negative rods the most susceptible was the Bacteroides vulgatus (MIC < 0.06 mg/ml). The strains from genus of Prevotella bivia and Prevotella buccalis were the lowest sensitive. The minimal inhibitory concentration for these strains was 1.0 mg/ml. But 47% of this strains was inhibited by concentrations in range < 0.06-0.25 mg/ml. Conclusions. The melissa oil showed high activity against all tested anaerobic bacteria, The Gram-positive bacteria were the most susceptible to tested oil than Gram-negative anaerobic rods.

STUDY OF CORIANDER SEED OIL (Coriandrum sativum L.)

2010 ◽  
Vol 13 (3) ◽  
pp. 29-38
Author(s):  
Ha Bich Phan ◽  
Thach Ngoc Le
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Seed Oil ◽  
Coriandrum Sativum ◽  
Conventional Heating ◽  
Geranyl Acetate ◽  
Physical And Chemical ◽  
Coriander Seed Oil ◽  
Coriander Seed

Coriandrum sativum L. belongs to the Apiaceae family, which is cultivated in Dong Nai province, in this paper its seed oil of was studied. The essential oil was extracted by hydrodistillation. We used two methods for activating of hydrodistillation: conventional heating and microwave irradiating. Its physical and chemical indexes were measured. The chemical composition of this oil was identified by GC/MS and quantified by GC/FID. Linalool (75.51-77.21 %), and geranyl acetate (15.64-12.79 %) were the main constituents of the oil which were obtained in 0,32-0.39 % yield. The biological activity of this oil was reported.

GC-MS, HPLC, TLC of Dorycnium herbaceum Tincture Species and Synergistic / Antagonist Effect Testing in Combination with Antibiotics

2017 ◽  
Vol 68 (2) ◽  
pp. 228-231 ◽  
Author(s):  
Renata Maria Varut ◽  
Luciana Teodora Rotaru
Keyword(s):  
Chemical Composition ◽  
Clavulanic Acid ◽  
Carboxylic Acids ◽  
Therapeutic Effect ◽  
Antagonistic Effect ◽  
Hydroalcoholic Extract ◽  
Low Concentrations ◽  
Amoxicillin Clavulanic Acid ◽  
Reference Strains ◽  
Antagonist Effect

The study objectives were to determine the chemical composition and the synergistic / antagonistic effect of the association between hydroalcoholic extract from the Dorycnii pentaphylli herba (DPH) and the antibiotics of choice, on five reference strains. The tincture contains flavonoids and polyphenol carboxylic acids in low concentrations. DPH has an antagonistic effect on three of the drugs tested (amoxicillin + clavulanic acid, levofloxacin, amikacin), the therapeutic effect being completely canceled and has no significant effect on two of them (ceftazidime, cefotaxime).

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