scholarly journals БІОЛОГІЧНЕ ОЧИЩЕННЯ ВОДНИХ ОБ’ЄКТІВ ВІД 137Cs

Author(s):  
O. M. Mikheyev ◽  
O. V. Lapan ◽  
S. M. Madzhd

A mobile bio plateau design has been developed for water’s bodies purification from radionuclides. To construct bio plateau chemically inert floating material was used as the substrate granular foam on top of which the seeds of higher terrestrial plants were placed. Constructed bio plateau on the 9th day of incubation were placed into tanks with a solution of 137Cs to study the cleaning efficiency of terrestrial plants to the aquatic environment. Once a day, the solution of 137Cs was poured into Marinelli's vessel to determine the specific activity of the radionuclide using a gamma spectrometer. A comparative study of the cleaning efficiency of terrestrial plants of the aquatic environment on the example of 137Cs ions has been carried out. The results of mathematical modeling of the dynamics of accumulation of radioactive cesium in the “experimental reservoir – bio plateau” system was presented.

ANRI ◽  
2021 ◽  
Vol 0 (1) ◽  
pp. 31-44
Author(s):  
Aleksey Vasil'ev ◽  
Aleksey Ekidin ◽  
Mariya Pyshkina ◽  
Georgiy Malinovskiy ◽  
Aleksandra Onischenko ◽  
...  

A method for non-destructive monitoring of the content of natural radionuclides in building materials has been developed. Spectrum measurements of gamma radiation are carried out with a pre-calibrated field gamma spectrometer. The calculation of the average specific activity of natural radionuclides in building materials is carried out by comparing the calculated flux density of unscattered gamma quanta normalized to the specific activity, and the experimentally measured count rates in the photopeak. calculated for the geometry of the room under study and the location of the detector. Application of the developed method makes it possible to estimate the average activity of natural radionuclides in building materials without destruction.


2019 ◽  
pp. 47-52
Author(s):  
Alexander Mikhailovich Okunev

The degree of contamination of the organism of wild animals with radionuclides is of particular importance in the framework of environmental monitoring and assessment of its safety, both for humans and for animals. However, the majority of domestic scientific works on this subject concerns farm animals, and the information relating to hunting animals is much less. This fact determined the relevance of our work, the purpose of which was to study the features of the accumulation of global technogenic and natural radionuclides in the meat of some wild animals in the TRANS-Urals. Experimental data on the content of technogenic radionuclides in the meat of hunting animals were obtained in the autumn-winter periods of 2014 – 2018. Analysis of the average data showed, that the content in the meat of wild animals cesium-137, depending on the animal species, ranged from 4.1 to 49.2; lead-210 – 0,7 – 4,3; and strontium-90 – 0,1 – 1,7 Bq/kg. The total beta- activity of radionuclides in the meat of wild animals varied, depending on the species, from 71.2 to 104.3 Bq/kg. At the same time, all the studied samples of meat corresponded to the norms established by SanPiN-2010 for this type of food product. Some increased accumulation of radioactive cesium (49.2 Bq/kg) and other anthropogenic radionuclides was observed in wild reindeer meat, and in the southern regions of the TRANS-Urals – in bear and wild boar meat (21.1 and 17.4 Bq/kg, respectively). In the muscle tissue of the roe deer the content of this radionuclide was slightly higher than that of the elk (14.7 and 12.2 Bq/kg, respectively). The lowest concentration of cesium-137 (4.1 Bq/kg) and other nuclides was found in beaver meat. The content of strontium-90 in the meat of all tested animals was at a minimum level, and the specific activity of lead-210 was higher than strontium-90 by 2 - 10 times, depending on the species, which indicates the accumulation of this radionuclide in forest ecosystems due to anthropogenic pressure and expansion of the technogenic zone of cities.  The results of studies have shown that differences in the accumulation of technogenic radionuclides in the meat of the hunting animals are largely related to the nature of feeding and composition of the diet of wild species, and are subject to significant fluctuations in accordance with the change in the volume and qualities of food resources in forest biotopes over the year.      


Author(s):  
Indra Saptiama ◽  
Herlina Herlina ◽  
Sriyono Sriyono ◽  
E. Sarmini ◽  
Abidin Abidin ◽  
...  

PEMBUATAN RADIONUKLIDA MOLIBDENUM-99 (99Mo) HASIL AKTIVASI NEUTRON DARI MOLIBDENUM ALAM UNTUK MEMPEROLEH TEKNESIUM-99m (99mTc). Pembatasan penggunaan uranium sebagai target untuk produksi 99mTc menyebabkan rumah sakit di Indonesia  kesulitan mendapatkan pasokan 99mTc. Saat ini 99mTc diperoleh dari 99Mo hasil fisi (pembelahan uranium).  Pembuatan radionuklida 99Mo dari aktivasi neutron  molibdenum alam (MoO3) di teras reaktor G.A Siwabessy digunakan sebagai metode alternatif untuk memperoleh 99mTc. Tujuan penelitian ini adalah untuk melakukan pembuatan radionuklida 99Mo dari aktivasi neutron molibdenum alam untuk memperoleh 99mTc. Serbuk MoO3 alam sebanyak 5 gram dikemas dalam ampul kuarsa dan dimasukkan ke dalam inner capsul selanjutnya dikemas menggunakan outer capsul sebagai bahan target. Bahan target diiradiasi di reaktor G.A Siwabessy selama 100 jam. Hasil perhitungan diperoleh aktivitas  99Mo sebesar 65 % dari nilai maksimum yang dapat diperoleh. MoO3 paska iradiasi dilarutkan dengan NaOH 4 M sehingga diperoleh larutan natrium molibdat (Na2MoO4). Radionuklida 99Mo dan 99mTc diukur menggunakan spektrometer gamma. Radionuklida 99Mo terdeteksi dalam produk larutan  Na2MoO4 dengan  aktivitas jenis 99Mo yang diperoleh sebesar 0,81 Ci 99Mo/g Mo.  Radionuklida anak luruh 99mTc dipisahkan dari radionuklida induk 99Mo menggunakan kolom pemisah yang berisi material berbasis zirkonium (MBZ) sebagai penyerap 99Mo. Radionuklida 99mTc hasil pemisahan diperoleh dalam bentuk natrium pertehnetat (Na99mTcO4).dengan recovery yang masih rendah yaitu sekitar 52 hingga 71 %.Kata kunci: Molibdenum, teknesium, radionuklida, pemisahan, iradiasi. PRODUCTION OF ACTIVATED  NEUTRON MOLYBDENUM-99 (99Mo) RADIONUCLIDE FROM NATURAL MOLYBDENUM TO OBTAIN TECHNETIUM-99m (99mTc).  Uranium usage restriction causes the hospitals in indonesia difficult to obtain the suply of  99mTc. At Present, 99mTc is obtanied from molybdenum as a uranium fission product. Production of 99Mo radionuclide resulted from neutron activated natural molybdenum (MoO3) in G.A Siwabessy reactor could be used  as a alternatif method for producing 99mTc. The aim of this research is synthesize of   99Mo radionuclide from neutron activated natural molybdenum  (MoO3) to obtain 99mTc. The five grams of  MoO3 powder was packed in a quartz ampule and inserted into inner capsule then also inserted into outer capsule as a target material. It was iradiated in G.A Siwabessy reactor for 100hours. Based on theoritical calculation, about 65 % of maximum 99Mo activity could be recovered. After Irradiation,  MoO3 was dissolved by NaOH 4 M solution so it was natrium molybdate (Na2MoO4) solution. 99Mo and 99mTc radionuclide were analyzed using gamma spectrometer. 99Mo radionuclide was detected on Na2MoO4 solution as product that had specific activity of 0.81 Ci 99Mo/ g Mo. 99mTc as daughter radionuclide was separated from 99Mo as parent radionuclide using separated column containing zirconium based material (ZBM) as 99Mo adsobent. 99mTc radionuclide has been succesfully separated using ZBM column although recovery of 99mTc  was quite low in which approximately 52 to 71 %. The 99mTc radionuclide was recovered in the form of sodium pertechnetate (NaTcO4) solution.Keywords: Molybdenum, technetium, radionuclide, separation, irradiation.


2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Séverine Mollard ◽  
Raphaelle Fanciullino ◽  
Sarah Giacometti ◽  
Cindy Serdjebi ◽  
Sebastien Benzekry ◽  
...  

1990 ◽  
Vol 269 (1) ◽  
pp. 93-100 ◽  
Author(s):  
J M F G Aerts ◽  
W E Donker-Koopman ◽  
S Brul ◽  
S Van Weely ◽  
M C Sa Miranda ◽  
...  

In Gaucher disease (glucosylceramide lipidosis), deficiency of glucocerebrosidase causes pathological storage of glucosylceramide, particularly in the spleen. A comparative biochemical and immunological analysis has therefore been made of glucocerebrosidase in spleens from normal subjects (n = 4) and from Gaucher disease patients with non-neuronopathic (n = 5) and neuronopathic (n = 5) phenotypes. The spleens from all Gaucher disease patients showed markedly decreased glucocerebrosidase activity. Discrimination of different phenotypes of Gaucher disease was not possible on the basis of the level of residual enzyme activity, or by measurements, using the immunopurified enzyme, of kinetic constants, pI or molecular mass forms. A severe decrease was found in the specific activity of glucocerebrosidase purified to homogeneity from the spleen of a patient with the non-neuronopathic phenotype of Gaucher disease, as compared with that of the enzyme purified from the spleen of a normal subject. This finding was confirmed by an immunological method developed for accurate assessment of the relative enzyme activity per molecule of glucocerebrosidase protein. The method revealed that the residual enzyme in the spleens of all investigated patients with a non-neuronopathic course of Gaucher disease had a more than 7-fold decreased activity of glucocerebrosidase (measured in the presence of taurocholate) per molecule of enzyme, and that the concentration of glucocerebrosidase molecules in the spleens of these patients was near normal. Observations made with immunoblotting experiments were consistent with these findings. In contrast, in the spleens of patients with neuronopathic phenotypes of Gaucher disease, the concentration of glucocerebrosidase molecules was severely decreased.


1981 ◽  
Author(s):  
F Jacks ◽  
B A Bradlow

Autoantibodies were solubilized from washed platelets by three successive freeze thaw cycles followed by sonication for 15 seconds at maximum intensity (MSE Mk 2). The disrupted platelets were centrifuged at 30,000g for 20 minutes and the supernatant was heated at 56°C for 30 minutes followed by centrifugation at 3,000g for 5 minutes. An aliquot of the supernatant was diluted with 0,01M phosphate buffered saline pH 7.4 to give a final concentration equivalent to about 5 × 106 platelets/ml. Fifty microlitres of the diluted extract was adsorbed onto the wells of a microtitre plate by incubation at room temperature for two hours. The wells were then washed three times with 0,01M phosphate buffered saline pH 7.4 containing 0,05% Tween (Tween-PBS) and the adsorbed antiplatelet IgG reacted with 50μl of an iodine-125 labelled affinity isolated goat antihuman (heavy chain specific) IgG, specific activity ≃ 50μCi/ μg (California Antibodies) for one hour at room temperature followed by three washes with Tween-PBS. A standard curve was constructed and run in parallel, in the dose range 0,l-100ng using purified human IgG (Miles Laboratories). The separated wells were counted in an automatic gamma spectrometer (Packard 3003) and the results calculated on a desk-top computer (Hewlet-Packard 9800) using a Rodbart weighted Logit-Log transformation (f = 0,996). A relatively poor arithmetic correlation was found between whole blood platelet counts and measured platelet bound IgG (r = -0,42), whereas a better correlation was obtained using a log-log plot (r = - 0,84).The results in ng/106 platelets were - Known ATP 133,2 ± 154, other autoimmune diseases with thrombocytopenia 212,0 ± 202, non-immune thrombocytopenia 18,6 ± 4,8, normal healthy controls 11,4 ± 3.7


1974 ◽  
Vol 46 (4) ◽  
pp. 501-510
Author(s):  
Manjusri Das ◽  
A. N. Radhakrishnan

1. A comparative study has been made of glycyl-l-leucine hydrolase activity in the soluble and particulate fractions of intestinal mucosa from monkey, guinea-pig, rabbit and rat. The specific activity of the soluble enzyme is very high in monkey and guinea-pig, and lower in rabbit and rat. The particulate enzymes from all the four species show low specific activities and form 1–10% of the total activity. 2. The pH optima in all cases lie in the range 7·6–7·8. The Km values of the substrate were similar for both soluble and particulate enzyme from monkey and guinea-pig, but in the rabbit and rat the Km value with the particulate enzyme was higher than with the soluble enzyme. 3. The particulate enzyme activity in all cases was the highest in the distal regions of the intestine, whereas the soluble enzyme showed maximal activity in the proximal and middle regions.


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