МОДЕЛЮВАННЯ ЧИСЕЛЬНОСТІ ГРИЗУНІВ У ЛІСОВИХ БІОТОПАХ ЗАХІДНОГО ПОДІЛЛЯ (НА ПРИКЛАДІ MYODES GLAREOLUS)

Monitoring and predicting the dynamics of abundance of species living in natural habitats is an important component stability analysis of ecosystem as well as dynamics and direction of change of biotic communities under global climate change and pressure of the alien species. The aim of the work was to build a matrix model and study the state of stabilisation of the dynamics of the bank vole population within the Leslie model. The object of the study was the population dynamics of Myodes glareolus Schreber, 1780 = Clethrionomys glareolus auct. The study is based on materials obtained during 2017–2019. This period covered one phase of the long-term population dynamics of the bank vole, named “population growth”. The research was carried out according to generally accepted methods. A total of 6400 trap-days were processed, and 358 forest fistulas were collected and studied. The intensity of harmful activity of rodents is due to the variability of the number of animals in the population. The quantitative population changes are the result of three factors: births, deaths, and migrations. The main condition for the existence of the species is the stability of the population, which is determined by the action of thecompensatory mechanisms. The growth phase of the bank vole lasted all three years of the research, the quantitative indicators were respectively: 2017 – 1.8 individuals per 100 trap-days; 2018 – 2.0 individuals per 100 trap-days; 2019 – 2.7 individuals per 100 trap-days. Low levels of the abundance in the spring of each year of the study, namely at the beginning of the breeding season (3.7 – 2.6 – 8.9 individuals per 100 trap-days). Favourable for the abundance growth was the sex ratio of the population (approximately 1:1), with some rise in the share of females, which decreases on the period of spring 2018 to autumn 2019). Some decrease in the share of immature individuals (4.5 – 3.9 – 3.1 %) is an indirect confirmation of the stability of puberty of animals with subsequent replenishment of the "stock", which led to accelerated reproduction and, consequently, provided prerequisites for further population growth. The causal mechanisms of population control established by us, without a doubt, can serve as a basis for further prognosis, of the number of pests in natural habitats. To predict population changes, the Leslie model, which is widely used in mathematical analyses of the abundance of both plant and animal groups, was chosen. The algorithm for building a matrix model, detailed in the article, has five following steps. The exponential nature of the actual and projected growth of the bank vole population during the five-year cycle (2017–2019 with a prognosis until 2023) revealed in the analysis can be explained not so much by the power of the species' reproductive potential as by the lack of the significant changes in habitat, caused by constant weather conditions, low individual mortality from predators and non-communicable diseases or other accidents. The application of the matrix model allowed to confirm the key role of the main compensatory mechanisms of population dynamics, as they contribute to the stabilisation of the population and, as a consequence, are an important condition for the existence of the species.

ВІКОВА СТРУКТУРА ПІДРОСТУ CARPINUS BETULUS L. У КУЛЬТУРФІТОЦЕНОЗАХ НАЦІОНАЛЬНОГО ДЕНДРОЛОГІЧНОГО ПАРКУ «СОФІЇВКА» НАН УКРАЇНИ

The issue of preserving the dendrological structure in historical parks requires the implementation of a number of agronomic measures aimed at ensuring the decorative and durability of plant communities. The plants of Carpinus betulus L. are included in the vast majority of deciduous plantations of the National Dendrological Park «Sofiyivka» of NASU, form the stands with the participation of Quercus robur L., Acer platanoides L., Acer campestre L. and Fraxinus excelsior L. The question of self-recovery of C. betulus plants in the park is relevant in connection with natural waste and active reduction of its viability identified over the past 10 years. The analysis of the success of natural regeneration of aboriginal plants in the cultural phytocenoses of the National Dendrological Park «Sofiyivka» of NASU has been conducted. As a result of the survey of the dendrological structure, viability of the young growth of the main structure-forming species under the stand was revealed. The prospects of replacing senile plants of C. betulus with the younger generation have been outlined. The young growth of C. betulus is 4.5% of the total number of viable undergrowth in the test plots, which is significantly less compared to A. platanoides – 64.5 % and A. campestre – 20. 7%. There is a sufficient number of viable virginile and young generative plants of C. betulus, which in future is able to form a stand. Prospects for natural regeneration of plants of the C. betulus species are average, similar in quantitative and age characteristics to plants of the Fraxinus excelsior L. The number of virginile plants is 0.5 %, and young generative – 0.1 %, compared with A. platanoides and A. campestre, the results are more than 10 times lower for virginile and 2 times – for young generative plants, which indicates a weak competitiveness of the young growth of C. betulus. Artificial phytocenoses with the participation of C. betulus are one of a few self-regenerating species in the park, as aboriginal species have a higher adaptive potential and are capable of successful renewal. Control over the ratio of species and quantitative composition of undergrowth, timely rational sanitary and landscape felling will create optimal conditions for growth and development of the young growth of C. betulus. Regular removal of aggressive plants of A. platanoides, A. campestre and F. excelsior will help preserve the existing structure of the stand in the historic part of the NDP «Sofiyivka».

ЕФЕКТИВНІСТЬ ДОБРИВА ПЛАНТАФОЛ У ПОСІВАХ СОЇ ЗА НЕСТАЧІ ЕЛЕМЕНТІВ ЖИВЛЕННЯ В ЧОРНОЗЕМІ ТИПОВОМУ

The agrochemical composition of chernozems typical of the agrobiological laboratory of Ternopil Volodymyr Hnatiuk National Pedagogical University has been studied and the economic expediency of using complex mineral fertilizer Plantafol 10.54.10 during cultivation of soybeans, to eliminate the shortage of some elements of mineral nutrition and increase crop productivity was analyzed. It was found that chernozems is characterized by a close to neutral exchange (pH 5.6) and hydrolytic (2.16 mg-eq. / 100 g) reaction, an increased amount of absorbed bases (19.0 mg-eq. / 100 g) and increased rate of saturation of bases (89.8 %), which is typical of agricultural lands with high carbonate content. Сhernozem soil has a low amount of easily hydrolyzed Nitrogen (102.0 mg / kg), an average supply of humus (2.63 %) and mobile Phosphorus (71.0 mg / kg) and a very high level of metabolic Potassium (189.0 mg / kg). Based on the specified agrochemical composition of the soil, taking into account the peculiarities of mineral nutrition of soybean plants, the importance for the formation of generative organs of Phosphorus and its ability to highly effective symbiotic nitrogen fixation in the study area, it is suggested to eliminate possible shortages of soil nutrients, in the first of Phosphorus, foliar fertilization with Plantafol 10.54.10. Complex mineral fertilizer Plantafol 10.54.10 contains Nitrogen – 10 %, Phosphorus – 54 %, Potassium – 10 %, as well as trace elements in chelated form EDTA – Boron 0.02 %, Iron – 0, 07 %, Manganese – 0.03 %, Zinc – 0.01 %, Copper – 0.005 %. It was found that double foliar fertilization with Plantafol 10.54.10 fertilizer at a dose of 3 kg / ha increases the yield of soybean grain cultivar Annushka, which is grown on chernozems typical of agrobiolaboratory, by 0.34 t / ha or 15.5 %. Yield growth was mainly due to a 16.8 % higher biological yield of aboveground mass and an increase in the number of beans on plants – by 15.3 %, seed weight – 16.0 %, total number of seeds – 11.4 %, as well as less pronounced increase in the height of attachment of the lower beans, the density and height of plants in the crop and the weight of 1000 seeds. The length of the fruits and their glazing did not change as affected by fertilizer. The obtained data allow to offer foliar fertilization with complex fertilizer Plantafol 10.54.10, as one of the elements of the system of soybean fertilization on chernozems with close to neutral reaction, increased amount of absorbed bases, low amount of easily hydrolyzed Nitrogen, average supply of humus and mobile Potassium and a very high level of exchangeable Potassium, which reduces the negative impact of deficiencies in the root nutrition of plants, increases crop productivity and provides additional income of 4078 UAH / ha.

РОЛЬ ЕКОЛОГІЧНИХ ФАКТОРІВ У ФОРМУВАННІ ПСЕВДОМЕЙОБЕНТОСУ (TEMPORARY MEIOFAUNA) ОДЕСЬКОГО РЕГІОНУ ЧОРНОГО МОРЯ

An analysis of long-term studies has made it possible to characterize the formation density of pseudomeiobenthos (temporary meiofauna) settlements depending on the substrate, depth, and seasons of the year in the Odessa Sea region of the northwestern Black Sea by the example of oligochaetes, polychaetes and juvenile mollusks. Their largest accumulations (the total density of settlements was 30865.8±5384.3 ind m-2) are characteristic of the ground sand/shell, the smallest for silty substrate (averaged 11705.5±1337.8 ind.m-2). The maximum indices of the total number of temporary meiofauna were recorded on depth of 10-15 m (20826.3±5010.4 ind.m-2). As shown by long-term studies, the average indicators of the total number of meiobenthos are highest in the winter period. The same applies to the density of the temporary component.

ВИДИ ПІДРОДИНИ NIMPHALINAE В ЗООЛОГІЧНИХ КОЛЕКЦІЯХ ТЕРНОПІЛЬСЬКОГО НАЦІОНАЛЬНОГО ПЕДАГОГІЧНОГО УНІВЕРСИТЕТУ ІМЕНІ ВОЛОДИМИРА ГНАТЮКА

The species diversity of the insects of Nimphalinae subfamily (Lepidoptera order), which are stored in the entomological collection of The Botany and Zoology Department of Ternopil Volodymyr Hnatiuk National Pedagogical University were studied. The insects were collected by the students during their teacher training and also by the amateur entomologists during 2000–2020. These insects are in the collections of The Botany and Zoology Department. The species composition of the collected insects was analyzed. There are 202 individuals of Nimphalinae subfamily. Identified insects belong to five genus (Vanessa, Inachis, Agаlis, Polygonia, Nymphalis). The largest number of individuals of this subfamily was calculated (Inachis io –75, Vanessa atalanta – 52, Cynthia cardui – 45). Also 13 individuals of Agаlis urticae and 12 individuals of Polygonia c-album were found. All species of Rhopalocera of European fauna are in The International Union for Conservation of Nature (IUCN) Red List of Threatened Species. Most of them are classified as Least Concern (LC). Widespread, numerous and common species of the Nimphalinae subfamily were found in the funds of The Botany and Zoology Department. Identified species belong to the LC category of The IUCN Red List. The Compton tortoiseshell (Nymphalis vaualbum) is included in The Red Data Book of Ukraine (2009). Its conservation status is invaluable. Nymphalis vaualbum was not found in the entomological collections of The Botany and Zoology Department of TNPU.

ВМІСТ ФОТОСИНТЕТИЧНИХ ПІГМЕНТІВ У ЛИСТКАХ ГРЕЧКИ ЗА ДІЇ БІОЛОГІЧНИХ ПРЕПАРАТІВ

The article presents the results of a field experiment to study the effects of different rates of the microbial preparation Diazobacterin under different methods of using the plant growth regulator Radostim (seed treatment before sowing and spraying crops) on the content of chlorophyll a and b in buckwheat leaves of Yelena variety, their sum and carotenoids. Determination of chlorophyll a and b, their sum and carotenoids was performed in selected samples of buckwheat leaves in the field conditions, in the phases of stem branching and the beginning of flowering according to the method described by V.F. Gavrylenko and T.V. Zhyhalova using a spectrophotometer. The pigment concentration was calculated from Holm-Wettstein's equations for 100 % acetone. It was found that the content of photosynthetic pigments in buckwheat leaves is conditioned by the weather, rates and methods of application of the studied microbial preparations and phases of plant development. In particular, the analysis of chlorophyll and carotenoid content in the early flowering phase of buckwheat plants showed a significant increase compared to the phase of stem branching, which may be due to increased physiological and biochemical processes in plants, against the background of improving mineral nutrition, growth and plant development. Thus, with the multiple use of the microbial preparation Diazobacterin in the rates of 150, 175 and 200 ml and the stimulator of plant growth Radostim in the rate of 250 ml / t for seed treatment before sowing, the content of chlorophylls a and b, their sum and carotenoids with increasing application of Diazobacterin increased and the excess relative to control ranged within: 29–31 % for chlorophyll a; 10–11 % for chlorophyll b; 24–26 % for their sum and 23–24 % for carotenoids. At the same time, with a complex application for pre-sowing seed treatment of a mixture of the microbial preparation Diazobacterin in the rate of 200 ml per hectare of seeds with the stimulator of plant growth Radostym in the rate of 250 ml / t followed by spraying on this background crops the stimulator of plant growth Radostim in the rate of 50 ml / ha buckwheat leaves form the highest content of chlorophyll a in comparison with the control (40 %); chlorophyll b (15 %); the sum of chlorophyll a + b (33 %) and carotenoids (29 %). These data indicate the creation of more favorable conditions for physiological and biochemical processes in plants, including photosynthesis, with the direct positive effect of which, functionally active pigment complex of the leaf apparatus of buckwheat is formed.

СТЕПАН КОCТИШИН. МЕЛОДІЯ СТАРОЇ ФІСГАРМОНІЇ. ЖИТТЯ НА ЗРІЗІ СТОЛІТЬ

In 2021, the Chernivtsi publishing house «Bukrek» published a book of memoirs of a famous Ukrainian scientist, physiologist, plant biochemist and ecologist, academician of the Academy of Sciences of Ukraine, doctor of biological sciences, professor, honoured worker of science and technology of Ukraine, honorary doctor of law of the University of Saskatchewan (Canada, 2010), former rector of Yurii Fedkovych Chernivtsi National University – Stepan Stepanovych Kostyshyn – «Stepan Kostyshyn. The melody of the old physharmonica. Life at the turn of centuries.». The book of memoirs is dedicated to the life and creative career of its author, his ups and downs, losses and victories. Stepan Kostyshyn wrote his book to parents, fellow villagers from the village of Zvyniach, Ternopil region, and graduates of Yurii Fedkovych Chernivtsi National University. Young Stepan Kostyshyn dreamed of becoming a geologist, but fate decided otherwise and in 1950 he became a student of the Agrobiology Department of the Faculty of Biology of Chernivtsi University. Work in a student research group, qualified lecturers instilled in the gifted student a thirst for knowledge and scientific research, and six years after graduation from the university Stepan Kostyshyn became a post-graduate student of the Department of Plant Physiology. The scientific supervisor of the young post-graduate student was a well-known scientist, Professor Molotkovskyi H. Kh. After defending his Candidate's dissertation, Stepan Stepanovych began his teaching and research activities at the university, firstly at the Department of Botany, later – Plant Physiology; he headed the problematic research laboratory of plant heterosis, and he worked as Vice Rector for Research for 15 years. In 1987, for the first time on a competitive basis, Kostyshyn S. S. was elected as a rector of Chernivtsi University and headed this famous university for 18 years. The life of two world geniuses of genetic science – Erwin Chargaff and Mykola Vavilov – is connected with the city of Chernivtsi. The world-famous discoverer of the DNA structure – the most outstanding discovery of the twentieth century – Erwin Chargaff was born on August 11, 1905 in the city of Chernivtsi and lived there until the First World War. And Mykola Vavilov, who gave the world the concept of centres of origin of cultivated plants and the law of homologous series of hereditary variability, ended his life in our city. This was his last expedition devoted to the search for relict spelt. From there he was taken directly to the NKVD cell in Lubianka. The author of the book was directly involved in perpetuating the memory of these world-famous scientists. The reviewed book will be extremely interesting for young people as life and the creative career of S. S. Kostyshyn is an example of how one’s hard work can bring great success in science and professional activity. It is of great interest to biologists, lecturers of higher educational establishments, as it contains invaluable information about the development of biological science in Bukovyna, the main milestones of the leading university of Ukraine – Yuriy Fedkovych Chernivtsi National University. In the book of memoirs, the author successfully interweaves events from his own biography in the outline of the development of Bukovyna University.

ГІСТОЛОГІЧНА ХАРАКТЕРИСТИКА ЗЯБЕР ТА НИРОК КАРАСЯ СРІБЛЯСТОГО З ЗАПОРІЗЬКОГО (ДНІПРОВСЬКОГО) ВОДОСХОВИЩА

In this article the histological structure of gills and kidneys of the Prussian carp is considered and researched. The research was conducted in the waters of the Zaporizhian (Dnipro) reservoir, namely in the Samara Bay and the lower part of the reservoir (near the village of Viiskove) during 2017–2019. Previous studies have revealed the excess of maximum permissible concentrations of heavy metals in the water of the Samara Bay compared to the lower part of the Zaporozhian reservoir. High levels of nickel and cadmium were observed in the Samara Bay. Statistically significant differences were found between the content of copper, zinc, nickel, lead and cadmium in the two areas of the reservoir (p

ОСОБЛИВОСТІ ФУНКЦІОНАЛЬНОГО СТАНУ ОРГАНІЗМУ І ОБРОБКИ СЕНСОРНОЇ ІНФОРМАЦІЇ В ОСІБ ЮНАЦЬКОГО ВІКУ

The aim of the work is to study the functional indicators of the cardiovascular and respiratory systems, indicators of the efficiency of processing of visual information and motor response in adolescents with different levels of functional reserves of the heart. We studied indicators of heart rate, blood pressure, Skibinsky index, Rosenthal’s test, the speed of the visual-motor reaction (simple and differentiated), the number of errors made during the examination, the analysis of the indicators of the correction test, the volume of operational visual memory, the dynamics of the analysis of Schulte tables, the volume and distribution of attention. The study demonstrated that the functional indicators of the cardiovascular system are within the permissible limits. However, the heart rate of individuals with a satisfactory level of heart efficiency tends to approach the upper physiological limit. Skibinsky’s index corresponds to a satisfactory level for the females. This index indicates the average potential of the external respiration system and the correspondence of activity with the blood circulatory system. For the analysis of Schulte tables, the individuals of the first group spent 161.8 s ± 10.37 s, which is significantly faster than the index of the second group. Studies have not shown a relationship between the level of heart performance and indicators of the distribution of attention. However, the indicators of the volume of operative visual memory in individuals of the first group are much better: 65 % of individuals have a high level (the same index in the second group is 20 %), the participants with a low level of operative visual memory are absent, in contrast to the second group. The speed of simple and differentiated sensorimotor reactions in individuals of the first group is higher than those in the second group by 11.4 % and 10.6 %, respectively. This indicates a sufficient level of plasticity of nervous processes and the efficiency of arousal development. The performance indicators of the cardiovascular and respiratory systems, the speed of reactions of the nervous system of the examined, the efficiency of processing sensory information indicate the maturity of the functional systems of the body and are the physiological basis for the formation of an adequate functional state of the body. Individuals with a Rufier index level above the average showed less tension in the work of the heart and an effective course of sensorimotor reactions, indicating a sufficient level of plasticity of nervous processes.

УЛЬТРАСТРУКТУРНІ ОСОБЛИВОСТІ НЕЙРО-ГЛІАЛЬНО-КАПІЛЯРНИХ ВІДНОСИН СА3 ПОЛЯ ГІПОКАМПА ЧЕРЕЗ 21 ДОБУ ЗА УМОВ ЕКСПЕРИМЕНТАЛЬНОЇ ТЕРМІЧНОЇ ТРАВМИ

Severe thermal injury leads to a number of morphofunctional changes that occur in violation of integrity or loss of skin in deep burns and endogenous intoxication on the background of which significant structural and metabolic disorders in all organs and systems of the affected organism are developed. This is manifested in a complicated symptom complex of burn disease. Changes in the central nervous system play an important role in the pathogenesis of burn injury, the nature and depth of morphofunctional changes in individual cerebral structures, in particular in the hippocampus (central organ of the limbic system, which is especially sensitive to various pathogens), after thermal lesions remain poorly studied. The aim of our study was to establish the features of submicroscopic changes in the structural components of the hippocampus after 21 days under conditions of experimental thermal injury. The experimental study was performed on white laboratory male rats. Third-degree burns were applied under thiopental-sodium anesthesia with copper plates heated in boiled water to a temperature of 97–100°C. The size of the affected area was 18–20% of the rats’ body epilated surface. Histological changes were studied on the 21st day from the beginning of the experiment. For electron microscopy, the obtained ultrathin sections were contrasted with uranyl acetate and lead citrate according to the Reynolds method. It was found that on the 21st day after the experimental thermal injury significant alternative changes in neurons, glial cells and the microcirculatory tract of the hippocampus were defined which developed on the background of excessive endogenous intoxication. Neurons are hypochromic and sharply hyperchromic, disorganized and have an altered shape; there are cell-free areas. Endoplasmic reticulum, Golgi complex, mitochondria are destructively changed. Degeneration of macroglial cells has been noticed. Their nuclei are high in electron density in which heterochromatin predominates. Destruction and lysis of organelles, lysosomes have also been found in the cytoplasm. These changes of astrocytic glia lead to impaired trophic function and the relation between astrocytes and blood capillaries. Hemocapillaries wall remodeling is manifested by the total edema, compaction of the basement membrane. The lumenal surface of the plasmolemma of endothelial cells is homogeneous, indistinctly contoured. The cytoplasm of endothelial cells is swollen, enlightened, and poor in organelles; there is a small number of micropinocytic bubbles and vesicles.