Synthesis of new fatty acid derivatives of oleanane and ursane triterpenoids and investigation of their in vitro cytotoxic effects on 3T3 fibroblast and PC3 prostate cancer cell linesLines

Prostate cancer is one of the cause of mortality and morbidity in men. High nutritional quality mushrooms have been consumed as food for a long time and Thanks to their bioactive components, they can be used in many fields such as pharmaceuticals, cosmetic products, dietary supplements and functional food production. The purpose of the research was to evaluate these derivatives against in vitro to obtain novel specific and effective anticancer agents against prostate cancer. In the study, Amanita caesarea, Sparassis crispa, Lepista nuda, Auricularia auricula, Tricholoma terreum and Lentinus tigrinus fungi were used. Anticancer activities of the compounds were evaluated in vitro by using MTT method against PC-3 and DU-143 (androgen-independent human prostate cancer cell lines) prostate cancer cell lines. Cisplatin was used as the positive sensitivity reference standard. The most effective among these fungus species biological activity against PC3 cancer cell line (IC50 = 327.34 µM), against DU-145 (IC50 = 459.19 µM).

Download Full-text

Antigenic expression of human metastatic prostate cancer cell lines for in vitro multiple-targeted α-therapy with 213Bi-conjugates

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/j.ijrobp.2004.04.035 ◽

2004 ◽

Vol 60 (3) ◽

pp. 896-908 ◽

Cited By ~ 13

Author(s):

Yong Li ◽

Syed M. Abbas Rizvi ◽

Julie M. Blair (nee Brown) ◽

Paul J. Cozzi ◽

Chang F. Qu ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Metastatic Prostate Cancer ◽

Prostate Cancer Cell ◽

Cancer Cell Lines ◽

Prostate Cancer Cell Lines ◽

Antigenic Expression

Download Full-text

Tissue-specific and androgen-regulated expression of human prostate-specific transglutaminase

Biochemical Journal ◽

10.1042/bj3150901 ◽

1996 ◽

Vol 315 (3) ◽

pp. 901-908 ◽

Cited By ~ 47

Author(s):

Hendrikus J. DUBBINK ◽

Nicole S. VERKAIK ◽

Peter W. FABER ◽

Jan TRAPMAN ◽

Fritz H. SCHRÖDER ◽

...

Keyword(s):

Prostate Cancer ◽

Mrna Expression ◽

Cancer Cell ◽

Prostate Cancer Cell ◽

Cancer Cell Line ◽

Human Prostate ◽

Tissue Type ◽

Human Prostate Cancer ◽

Human Prostate Cancer Cell

Transglutaminases (TGases) are calcium-dependent enzymes catalysing the post-translational cross-linking of proteins. In the prostate at least two TGases are present, the ubiquitously expressed tissue-type TGase (TGC), and a prostate-restricted TGase (TGP). This paper deals with the molecular cloning and characterization of the cDNA encoding the human prostate TGase (hTGP). For this purpose we have screened a human prostate cDNA library with a probe from the active-site region of TGC. The largest isolated cDNA contained an open reading frame encoding a protein of 684 amino acids with a predicted molecular mass of 77 kDa as confirmed by in vitro transcription–translation and subsequent SDS/PAGE. The hTGP gene was tissue-specifically expressed in the prostate, yielding an mRNA of approx. 3.5 kb. Furthermore, a 3-fold androgen-induced up-regulation of hTGP mRNA expression has been demonstrated in the recently developed human prostate cancer cell line, PC346C. Other well established human prostate cancer cell lines, LNCaP and PC-3, showed no detectable hTGP mRNA expression on a Northern blot. The gene coding for prostate TGase was assigned to chromosome 3.

Download Full-text