scholarly journals Descemeta membrānas endotēlija keratoplastika: nākamās paaudzes sekvenēšanas metagenomiski optimizētu audu sagatavošana un ķirurģijas tehnika. Promocijas darba kopsavilkums

2021 ◽  
Author(s):  
◽  
Davide Borroni ◽  
Keyword(s):  
Organ Culture ◽  
Diagnostic Method

Pētījumā tika analizēta optimizācija, pielietojot metagenomiku radzenes audu saglabāšanā. Nosaukums: Nākamās paaudzes sekvencēšana mikroorganismu noteikšanai cilvēka donora radzenes audu saglabāšanas vidē. Mērķis: Ar nākamās paaudzes sekvencēšanas metodi noteikt mikroorganismu klātbūtni cilvēka donora radzenes audu uzglabāšanas vidē. Materiāli un metodes: Kopā tika izmantoti septiņi paraugi no orgānu kultūras (no angļu val. – organ culture (OC)) grupas (Cornea Max, Eurobio, Les Ulis, Francija) ar vienu kontroli (sterila barotne bez radzenes audiem) un septiņi paraugi no hipotermiskās uzglabāšanas grupas (Cornea Cold, Eurobio) ar vienu kontroli. Pirms paraugu savākšanas radzenes audus ievietoja attiecīgajās uzglabāšanas vietās uz 14 dienām. Uzglabāšanas vide (2 ml) no katra parauga tika ievietota mēģenēs, kas nesatur RNS-āzi, un tika nosūtīta 16S un 18S ribosomu RNS sekvencēšanai. Vienlaikus 1 ml barotnes parauga tika izmantots konvencionālajai diagnostikas metodei (no angļu val. – conventional diagnostic method (CDM)), izmantojot Bactec instrumentus. Rezultāti: Gan OC, gan hipotermiskās uzglabāšanas un kontroles paraugos visbiežāk novēroja Pseudomonas, Comamonas, Stenotrophomonas, Alcanivorax, Brevundimonas un Nitrobacter ģintis. Acidovorax, Acetobacter un Hydrogenophilus ģintis tika atklātas galvenokārt hipotermiskās uzglabāšanas grupā. Visbiežāk konstatētais sēņu patogēns piederēja Malassezia ģintij, kas tika atrasts abās uzglabāšanas vidēs. Ar CDM visi paraugi bija negatīvi attiecībā uz mikroorganismiem. Secinājumi: Metagenomika nodrošina pilnīgu taksonomisko profilu noteikto organismu genomiskajam materiālam, tādējādi sniedzot daudz plašāku mikrobioloģiskās diagnostikas pieeju nekā CDM. Metodes izmaksu un apgrozījuma laika dzīvotspējīgu organismu noteikšanai samazināšana palīdzētu šo tehnoloģiju ieviest ikdienas klīniskajā praksē.

Freeze-etch observations on the tight junctions of sertoli cells grown in organ culture with FSH

1978 ◽  
Vol 36 (2) ◽  
pp. 340-341
Author(s):  
Rita Meyer ◽  
Zoltan Posalaky ◽  
Dennis Mcginley
Keyword(s):  
Organ Culture ◽  
Tight Junctions ◽  
Sertoli Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Barrier Function ◽  
Cell Junction ◽  
Intramembranous Particles ◽  
Junctional Complexes ◽  
Oriented Parallel

The Sertoli cell tight junctional complexes have been shown to be the most important structural counterpart of the physiological blood-testis barrier. In freeze etch replicas they consist of extensive rows of intramembranous particles which are not only oriented parallel to one another, but to the myoid layer as well. Thus the occluding complex has both an internal and an overall orientation. However, this overall orientation to the myoid layer does not seem to be necessary to its barrier function. The 20 day old rat has extensive parallel tight junctions which are not oriented with respect to the myoid layer, and yet they are inpenetrable by lanthanum. The mechanism(s) for the control of Sertoli cell junction development and orientation has not been established, although such factors as the presence or absence of germ cells, and/or hormones, especially FSH have been implicated.

IN VITRO METHODS FOR THE STUDY OF THE ADENOHYPOPHYSIAL FUNCTIONS TO SECRETE GONADOTROPHIN

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S27-S40 ◽  
Author(s):  
T. Kobayashi ◽  
T. Kigawa ◽  
M. Mizuno ◽  
T. Watanabe
Keyword(s):  
Cell Culture ◽  
Organ Culture ◽  
Cultured Cells ◽  
Cell Sheet ◽  
Short Term ◽  
Hypothalamic Extract ◽  
Numerous Cell ◽  
Single Cell Culture ◽  
Almost All

ABSTRACT There are several in vitro methods to analyse the function of the adenohypophysis or the mechanisms of its regulation. The present paper deals with single cell culture, organ culture and short term incubation techniques by which the morphology and gonadotrophin-secreting function of the adenohypophysis were studied. In trypsin-dispersed cell culture, the adenohypophysial cells showed extensive propagation to form numerous cell colonies and finally develop into a confluent monolayer cell sheet covering completely the surface of culture vessels. Almost all of the cultured cells, however, became chromophobic, at least at the end of the first week of cultivation, when gonadotrophin was detectable neither in the culture medium nor in the cells themselves. After the addition of the hypothalamic extract, gonadotrophin became detectable again, and basophilic or PAS-positive granules also reappeared within the cells, suggesting that the gonadotrophs were stimulated by the extract to produce gonadotrophin. In organ culture and short term incubation, the incorporation of [3H] leucine into the adenohypophysial cells in relation to the addition of hypothalamic extract was examined. It was obvious that the ability to incorporate [3H] leucine into the gonadotrophs in vitro was highly dependent upon the presence of the hypothalamic extract.

A method of technical diagnostics for offshore structures

1994 ◽  
Vol 16 (2) ◽  
pp. 43-48
Author(s):  
Do Son
Keyword(s):  
Diagnostic Method ◽  
Joint Venture ◽  
Oil And Gas ◽  
Residual Life ◽  
Life Time ◽  
Offshore Structures ◽  
Technical Diagnostics ◽  
Offshore Platforms ◽  
South Vietnam ◽  
Local Destruction

This paper describes the results of measurements and analysis of the parameters, characterizing technical state of offshore platforms in Vietnam Sea. Based on decreasing in time material characteristics because of corrosion and local destruction assessment on residual life time of platforms is given and variants for its repair are recommended. The results allowed to confirm advantage of proposed technical diagnostic method in comparison with others and have been used for oil and gas platform of Joint Venture "Vietsovpetro" in South Vietnam.

Calcium Ameliorates Renal Cyst Growth in Metanephric Organ Culture: A Morphological Study

2012 ◽  
Vol 31 (3) ◽  
pp. 285-293 ◽  
Author(s):  
Nidhi Mahajan ◽  
Tarundeep Kaur ◽  
Veena Puri ◽  
S.K. Singla ◽  
Vivekanand Jha ◽  
...  
Keyword(s):  
Organ Culture ◽  
Morphological Study ◽  
Renal Cyst ◽  
Cyst Growth

Evaluation of a new diagnostic method for quantification of EBV and HHV-6 viral load in bronchoalveolar lavage

2019 ◽  
Vol 31 (3) ◽  
Author(s):  
Jessica Montemezzo ◽  
Filippo Patrucco ◽  
Francesco Gavelli ◽  
Linda Ferrari ◽  
Giulia Faolotto ◽  
...  
Keyword(s):  
Viral Load ◽  
Bronchoalveolar Lavage ◽  
Diagnostic Method
Sign in / Sign up

Export Citation Format

Share Document