The aim of this study was to assess the prevalence of serological and molecular biological markers of viral hepatitis B and C among conditionally healthy residents of South Vietnam. The study material was represented by 397 blood serum samples collected from conditionally healthy residents of the Southern Vietnam. The ELISA examination for HBV and HCV markers occurrence involved HBsAg, anti-HBs IgG, anti-HBcore IgG, and anti-HCV qualitative determination. For HBV DNA and HCV RNA detection, nucleic acids were extracted from serum blood, and viruses presence test were executed by real-time PCR with hybridisation fluorescence detection. Amplification and subsequent sequencing of HBV and HCV were performed using nested PCR with pair’s overlapping primers jointly flanking the target regions. The analysis of the overall prevalence of serological markers showed that among the conditionally healthy individuals HBsAg and anti-HCV antibodies were detected in 12.3% (95% CI: 9.27%-15.99%) and 3.27% (95% CI: 1.76%-5.53%) of individuals, respectively. The prevalence of HBsAg in men (19.1%) significantly exceeded that in women (5.9%), χ2 = 14.688 with p = 0.0001, df = 1, calculated odds ratio OR = 3.751 (95% CI: 1.892-7.439). Among conditionally healthy patients, taking into account HBsAg-positive and negative samples, DNA HBV was detected in 26.95% (95% CI: 22.65%-31.6%). Phylogenetic analysis of HBV showed that subtype B4 prevalence is 64.49%, subtypes C1 – 14.95%, B2 - 9.35%, C2 – 6.54%, C3 – 0.93%, and C5 (3.74%) were also identified. HCV RNA was detected in 7 samples, which accounted for 1.76% (95% CI: 0.71%-3.6%). Phylogenetic analysis showed that all HCV isolates belong to genotype 6, subtype 6a (100%).It should be noted that the results obtained by us for conventionally healthy patients cannot be considered as population data, since we estimated the prevalence of viral hepatitis markers in the population visiting hospitals, while some socioeconomic or occupational groups may have more reasons to visit hospitals. than others. Thus, these samples may represent individuals with a higher risk of infection in general, and therefore may not be representative of the population.