Determination of phenylephrine hydrochloride and Amoxicillin in binary mixture using Derivative Spectrophotometry methods

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Aseel M Aljeboree ◽  
Abbas Noor Alshirifi
Keyword(s):  
Binary Mixture ◽  
Pharmaceutical Formulations ◽  
Mutual Interference ◽  
Derivative Spectrophotometry ◽  
Simultaneous Estimation ◽  
Second Derivative ◽  
Derivative Spectrum ◽  
Phenylephrine Hydrochloride ◽  
First Derivative

A simple, precise and economical procedure for the simultaneous estimation of Phenylephrine Hydrochloride (PHE) and Amoxicillin (AMX) in formulation has been developed. The absorbance values of first derivative spectrum 228,258 nm andm 241 nm and second derivative spectrum 238, 277 nm and 226 nm was used for the estimation of PHE and AMX, respectively without mutual interference. This method obeyed beerand#39;s law in the concentration range mixing of 2-150mgL -1 PHE with 0,20,100 mgL -1 AMX and 2-240 mgL -1 AMX with 0,20,100 mgL -1 PHE and the second derivative depends on first derivative of the ratios spectra. The proposed methods are extensively validated. All the described methods can be readily utilized for analysis of pharmaceutical formulations.

scholarly journals Determination of Metformin Hydrochloride and Glyburide in an Antihyperglycemic Binary Mixture Using High-Performance Liquid Chromatographic-UV and Spectrometric Methods

2010 ◽  
Vol 93 (1) ◽  
pp. 133-140 ◽  
Author(s):  
Hesham Salem
Keyword(s):  
Binary Mixture ◽  
High Performance ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Metformin Hydrochloride ◽  
Second Derivative ◽  
Zero Crossing ◽  
First Derivative

Abstract Three methods were developed for simultaneous determination of metformin hydrochloride and glyburide in an antihyperglycemic binary mixture without previous separation. In the first method, a reversed-phase HPLC column with acetonitrilewater (60 + 40, v/v) mobile phase at 0.9 mL/min flow rate was used to separate both compounds, with UV detection at 254 nm. Linearity was obtained in the concentration range of 0.060.24 µg/mL for glyburide and 1.56.0 µg/mL for metformin hydrochloride. The second method depended on first- and second-derivative UV spectrometry with zero-crossing measurements. The first-derivative amplitude at 261 nm was selected for the assay of glyburide, and the second-derivative amplitude at 235 nm was selected for the assay of metformin hydrochloride. The third method depended on measuring the first derivative of the ratio-spectra at 241 nm for glyburide and 227 nm for metformin hydrochloride. For the second and third methods, Beer's law was obeyed in the range of 1055 µg/mL for glyburide and 20200 µg/mL for metformin. The proposed methods were extensively validated and applied for the analysis of some pharmaceutical formulations containing binary mixtures of the mentioned drugs.

scholarly journals Simultaneous Determination of Dapsone and Pyrimethamine by Derivative Spectrophotometry in Pharmaceutical Formulations

2003 ◽  
Vol 86 (2) ◽  
pp. 241-245 ◽  
Author(s):  
M Inés Toral ◽  
Andrés Tassara ◽  
César Soto ◽  
Pablo Richter
Keyword(s):  
Simultaneous Determination ◽  
Signal To Noise Ratio ◽  
Pharmaceutical Formulations ◽  
Derivative Spectrophotometry ◽  
Fast Method ◽  
Signal To Noise ◽  
Zero Crossing ◽  
First Order ◽  
First Derivative

Abstract A simple and fast method was developed for the simultaneous determination of dapsone and pyrimethamine by first-order digital derivative spectrophotometry. Acetonitrile was used as a solvent to extract the drugs from the pharmaceutical formulations, and the samples were subsequently evaluated directly by digital derivative spectrophotometry. The simultaneous determination of both drugs was performed by the zero-crossing method at 249.4 and 231.4 nm for dapsone and pyrimethamine, respectively. The best signal-to-noise ratio was obtained when the first derivative of the spectrum was used. The linear range of determination for the drugs was from 6.6 × 10−7 to 2.0 × 10−4 and from 2.5 × 10−6 to 2.0 × 10−4 mol/L for dapsone and pyrimethamine, respectively. The excipients of commercial pharmaceutical formulations did not interfere in the analysis. Chemical and spectral variables were optimized for determination of both analytes. A good level of repeatability, 0.6 and 1.7% for dapsone and pyrimethamine, respectively, was observed. The proposed method was applied for the simultaneous determination of both drugs in pharmaceutical formulations.

scholarly journals Simultaneous Determination of Estradiol and Medroxyprogesterone Acetate in Pharmaceutical Formulations by Second-Derivative Spectrophotometry

2002 ◽  
Vol 85 (4) ◽  
pp. 883-888 ◽  
Author(s):  
M Inés Toral ◽  
César Soto ◽  
Pablo Richter ◽  
Ana E Tapai
Keyword(s):  
Simultaneous Determination ◽  
Medroxyprogesterone Acetate ◽  
Signal To Noise Ratio ◽  
Pharmaceutical Formulations ◽  
Derivative Spectrophotometry ◽  
Fast Method ◽  
Second Derivative ◽  
Zero Crossing ◽  
Relative Standard

Abstract This paper reports a simple and fast method for the simultaneous determination of estradiol (ED) and medroxyprogesterone acetate (MP) in pharmaceutical formulations by second-derivative spectrophotometry. Methanol was used to extract the drugs from formulations, and subsequently the extracts were evaluated directly by derivative spectrophotometry. The drugs were determined simultaneously by using the graphic method at 297.4 nm for ED and the zero-crossing method at 273.4 nm for MP. If both compounds are present together in a sample, it is possible to quantitate one in the presence of the other. The best signal-to-noise ratio was found when the second derivative of the spectrum was used. The linear ranges for determination of the drugs were 4.7 × 10−6 to 1.6 × 10−4 and 7.2 × 10−6 to 2.0 × 10−4 mol/L for ED and MP, respectively. The ingredients commonly found in commercial pharmaceutical formulations do not interfere with the determination. Chemical and spectral variables were optimized for the determination of both analytes. Good levels of repeatability (relative standard deviation), 1.4 and 1.9%, were obtained for ED and MP, respectively. The proposed method was applied to the determination of these drugs in pharmaceutical formulations.

scholarly journals VALIDATED SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF CINNARIZINE IN BINARY MIXTURE WITH PARACETAMOL IN BULK AND TABLETS

2021 ◽  
pp. 161-166
Author(s):  
AHMED M AL-GHANI ◽  
ANES AM THABET
Keyword(s):  
Quality Control ◽  
Second Derivative ◽  
Derivative Spectrum ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Derivative Method ◽  
Analytical Tools ◽  
Second Derivative Method

Objectives: The aim of this work was to develop and validate new, simple, accurate, and selective spectrophotometric methods (derivative and derivative ratio spectrophotometric methods) for the determination of these drugs. These methods can be used as analytical tools in routine examination in quality control laboratories. Methods: The first method was derivative method in which the first derivative method (1D) for determination of PCM and the second derivative method (2D) for determination of CIN. The second method was the first derivative ratio spectrophotometric method (1DD) for determination of CIN and PCM. Results: In first method, the first derivative spectrum (1D) of PCM where PCM was determined by measuring the amplitude of the valley at 235 nm while CIN showed zero crossing spectrum, and the second derivative spectrum (2D) of CIN where CIN was determined by measuring the amplitude of the peak at 287.5 nm while PCM showed a zero value. In the second method, the first derivative ratio spectrophotometry (1DD) for CIN and PCM determination, where the amplitude at 290 and 291 nm, was selected for the determination of CIN and PCM, respectively. Conclusions: The developed methods were applied for the determination of the cited drugs in tablets containing binary drug mixture. The methods are simple and precise and can be used for routine analysis of the labeled drugs in combined dosage forms in quality control laboratories.

scholarly journals Determination of Attapulgite and Nifuroxazide in Pharmaceutical Formulations by Sequential Digital Derivative Spectrophotometry

2004 ◽  
Vol 87 (6) ◽  
pp. 1323-1328 ◽  
Author(s):  
M Inés Toral ◽  
Maximiliano Paine ◽  
Patricio Leyton ◽  
Pablo Richter
Keyword(s):  
Pharmaceutical Formulations ◽  
Derivative Spectrophotometry ◽  
Second Derivative ◽  
Zero Crossing ◽  
Sequential Determination ◽  
Relative Standard ◽  
Naoh Solution ◽  
Second Derivative Spectra ◽  
Hydrolysis Of

Abstract A new method for the sequential determination of attapulgite and nifuroxazide in pharmaceutical formulations by first-and second-derivative spectrophotometry, respectively, has been developed. In order to obtain the optimal conditions for nifuroxazide stability, studies of solvent, light, and temperature effects were performed. The results show that a previous hydrolysis of 2 h in 1.0 × 10–1M NaOH solution is necessary in order to obtain stable compounds for analytical purposes. Subsequently, the first-and second-derivative spectra were evaluated directly in the same samples. The sequential determination of the drugs can be performed using the zero-crossing method; the attapulgite determination was carried out using the first derivative at 278.0 nm and the nifuroxazide determination, using the second derivative at 282.0 nm. The determination ranges were 5.7 × 10–6–1.0 × 10–4 and 3.7 × 10–8 –1.2 × 10–4M for attapulgite and nifuroxazide, respectively. Repeatability (relative standard deviation) values of 1.2 and 3.0% were observed for attapulgite and nifuroxazide, respectively. The ingredients commonly found in commercial pharmaceutical formulations do not interfere. The proposed method was applied to the determination of these drugs in tablets. Further, infrared spectroscopy and cyclic voltammetry studies were carried out in order to obtain knowledge of the decomposition products of nifuroxazide.

scholarly journals SIMULTANEOUS DETERMINATION OF PARACETAMOL AND TAPENTADOL IN TABLETS BY RATIO SPECTRA DERIVATIVE SPECTROPHOTOMETRY

2021 ◽  
Vol 12 (9) ◽  
pp. 27-32
Author(s):  
Shah Esha Bhavin ◽  
Gajjar Anuradha
Keyword(s):  
Absorption Spectrum ◽  
Binary Mixture ◽  
Simultaneous Determination ◽  
Derivative Spectrophotometry ◽  
Pharmaceutical Tablets ◽  
First Derivative ◽  
Standard Spectrum ◽  
Ich Guidelines ◽  
Relative Standard

The application of the ratio spectra derivative spectrophotometry to the simultaneous determination of Paracetamol (PCM) and Tapentadol (TAP) in combined pharmaceutical tablets is presented. The spectrophotometric procedure is based on the use of the first derivative of the ratio spectra obtained by dividing the absorption spectrum of the binary mixtures by a standard spectrum of one of the compounds. The first derivative amplitudes were measured at 220 and 232 nm for the assay of TAP and PCM, respectively. Calibration graphs were established for 1-5 μg mL-1 for TAP and 6.5-32.5 μg mL-1 for PCM in binary mixture. The detection limits for TAP and PCM were found 0.098 and 0.595 μg mL-1, respectively, while the quantification limits were 0.298 μg mL-1 for TAP and 1.805 μg/ml for PCM. The relative standard deviations were found to be less than 2%, indicating reasonable repeatability of both methods. The proposed methods were hence validated as per ICH guidelines and successfully applied to the determination of these drugs in commercial tablets.

scholarly journals Three Different Spectrophotometric Methods for Simultaneous Determination of Pyriproxyfen and Chlorothalonil Residues in Cucumber and Cabbage Samples

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Shilan A. Omer ◽  
Nabil A. Fakhre
Keyword(s):  
Simultaneous Determination ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
The Third ◽  
Mean Centering ◽  
Relative Standard ◽  
One Way Anova

In this study, three simple and accurate spectrophotometric methods for simultaneous determination of pyriproxyfen and chlorothalonil residues in cucumbers and cabbages grown in experimental greenhouse were studied. The first method was based on the zero-crossing technique measurement for first and second derivative spectrophotometry. The second method was based on the first derivative of the ratio spectra. However, the third method was based on mean centering of ratio spectra. These procedures lack any previous separation steps. The calibration curves for three spectrophotometric methods are linear in the concentration range of 1–30 μg·mL−1 and 0.5–7 μg·mL−1 for pyriproxyfen and chlorothalonil successively. The recoveries ranged from 82.12–97.40% for pyriproxyfen and 81.51–97.04% for chlorothalonil with relative standard deviations less than 4.95% and 5.45% in all instances for pyriproxyfen and chlorothalonil, respectively. The results obtained from the proposed methods were compared statistically by using one-way ANOVA, and the results revealed there were no significant differences between ratio spectra and mean centering methods with the zero-crossing technique. The proposed methods are successfully applied for the simultaneous estimation of the residue of both pesticides in cucumber and cabbage samples.

DETERMINATION OF TELMISARTAN HYDROCHLORIDE AND HYDROCHLOROTHIAZIDE IN BINARY MIXTURE AND PHARMACEUTICAL TABLET DOSAGE FORM BY FIRST DERIVATIVE RATIO SPECTROPHOTOMETRIC METHOD

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (11) ◽  
pp. 57-64
Author(s):  
V Thakur ◽  
◽  
S. J. Daharwal
Keyword(s):  
Binary Mixture ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
First Derivative ◽  
Pharmaceutical Tablet ◽  
Study Results ◽  
Recovery Study ◽  
Tablet Dosage

New first derivative ratio spectrophotometric method was developed for simultaneous estimation of telmisartan hydrochloride (TELM) and hydrochlorothiazide (HCTZ) in combined tablet dosage form without prior separation. The amplitude in the first derivative of the corresponding ratio spectra at 263.2 nm and 278.4 nm of TELM and for HCTZ at 287.2 nm and 304.8 nm were selected for the determination. The calibration graphs were established in the range of 1-10μg/mL and 2-15 μg/mL of TELM and HCTZ, respectively. The percentage recoveries of binary mixture of TELM were found to be 97.81±1.05 (263.2nm) and 96.60±1.91 (278.4nm) and for HCTZ were found to be 97.47±1.41 (287.2), 98.16± 1.45 (304.8nm) by proposed method. This method was successfully applied for the analysis of commercial tablet formulation, with no interference from excipients as indicated by the recovery study results. The proposed method was validated, statistically analyzed, compared with reported HPLC method and can be successfully applied for the routine analysis.

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