Ruminal digestion and glycosyl linkage patterns of cell wall components from leaf and stem fractions of alfalfa, orchardgrass, and wheat straw

1994 ◽  
Vol 72 (5) ◽  
pp. 1362-1374 ◽  
Author(s):  
Leslie D. Bourquin ◽  
George C. Fahey
1994 ◽  
Vol 46 (3-4) ◽  
pp. 331-342 ◽  
Author(s):  
Edith Yosef ◽  
D. Ben-Ghedalia ◽  
J. Miron ◽  
A. Huttermann ◽  
A. Majcherczyk ◽  
...  

1988 ◽  
Vol 111 (3) ◽  
pp. 503-511 ◽  
Author(s):  
P. T. Doyle ◽  
H. Dove ◽  
M. Freer ◽  
F. J. Hart ◽  
R. M. Dixon ◽  
...  

SummaryWeaned lambs of mean weight 25 kg were offered a diet of mature oaten hay or hay supplemented with a pelleted mixture of oat grain and sunflower meal (2:1), at one of three rates, for 86 days. The effect of the supplement on the voluntary intake of hay was measured during the first 20 days when feed was offered twice daily (Expt 1), after which the effects on ruminal and post-ruminal digestion were investigated under continuous feeding conditions (Expt 2).In Expt 1 the first increment of supplement increased the total intake of organic matter (OM) but increasing the supplement further, up to 510 g D.M., had no additional effect. The voluntary intake of oaten hay was not significantly reduced by the lowest rate of supplementation but at higher rates was depressed at a mean rate of 92 g/100 g supplement. Rates of change in fasted weight on the four treatments were —63, —5, 21 and 45 g/day, respectively.In Expt 2, where the rates of hay intake were held at 85% of those achieved in Expt 1, the first increment of supplement increased the pool size of OM and cell wall components in the reticulorumen by about 50%. It also increased their outflow rates at the abomasum by 24% and 33%, respectively, but significantly decreased the fractional outflow rate and fractional digestion rate of cell wall components. Supplementation decreased the proportion of apparent OM digestion that occurred in the reticulo-rumen from 76% to 65%. The presence of supplement doubled the ammonia pool in the rumen and increased the abomasal flow on non-ammonia nitrogen (NAN) and microbial NAN by 70%. Estimates of the amounts of crude protein apparently digested in the intestines (DCPi) increased linearly with proportion of supplement in the diet. However, the apparent digestibility of the hay was decreased, rather than increased, by the supplement. Although higher rates of supplement did not significantly change the elevated rumen pools of OM and cell wall components, there was a consistent tendency for these to decrease.The results are consistent with the view that the intake of unsupplemented hay was limited by its low nitrogen content and the intake of supplemented hay may have been limited primarily by the capacity of the reticulo-rumen, although other factors were increasingly involved at higher rates of supplementation.


1995 ◽  
Vol 4 (2) ◽  
pp. 127-145 ◽  
Author(s):  
Runcang Sun ◽  
J.Mark Lawther ◽  
W.B. Banks

2013 ◽  
Vol 131 ◽  
pp. 226-234 ◽  
Author(s):  
Zara Merali ◽  
Justin D. Ho ◽  
Samuel R.A. Collins ◽  
Gwénaëlle Le Gall ◽  
Adam Elliston ◽  
...  

1993 ◽  
Vol 64 (5) ◽  
pp. 505-511 ◽  
Author(s):  
Masahiro YAMADA ◽  
Haruki KITAZAWA ◽  
Junko UEMURA ◽  
Tadao SAITOH ◽  
Takatoshi ITOH

2021 ◽  
Vol 22 (3) ◽  
pp. 1169
Author(s):  
Yuhan Chang ◽  
Chih-Chien Hu ◽  
Ying-Yu Wu ◽  
Steve W. N. Ueng ◽  
Chih-Hsiang Chang ◽  
...  

Bacterial infection in orthopedic surgery is challenging because cell wall components released after bactericidal treatment can alter osteoblast and osteoclast activity and impair fracture stability. However, the precise effects and mechanisms whereby cell wall components impair bone healing are unclear. In this study, we characterized the effects of lipopolysaccharide (LPS) on bone healing and osteoclast and osteoblast activity in vitro and in vivo and evaluated the effects of ibudilast, an antagonist of toll-like receptor 4 (TLR4), on LPS-induced changes. In particular, micro-computed tomography was used to reconstruct femoral morphology and analyze callus bone content in a femoral defect mouse model. In the sham-treated group, significant bone bridge and cancellous bone formation were observed after surgery, however, LPS treatment delayed bone bridge and cancellous bone formation. LPS inhibited osteogenic factor-induced MC3T3-E1 cell differentiation, alkaline phosphatase (ALP) levels, calcium deposition, and osteopontin secretion and increased the activity of osteoclast-associated molecules, including cathepsin K and tartrate-resistant acid phosphatase in vitro. Finally, ibudilast blocked the LPS-induced inhibition of osteoblast activation and activation of osteoclast in vitro and attenuated LPS-induced delayed callus bone formation in vivo. Our results provide a basis for the development of a novel strategy for the treatment of bone infection.


Author(s):  
Alberto García-Iruela ◽  
Luis García Esteban ◽  
Francisco García Fernández ◽  
Paloma de Palacios ◽  
Alejandro B. Rodriguez-Navarro ◽  
...  

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