1632 Inulin as prebiotic for Lactobacillus salivarius and Enterococcus faecium with probiotic potential in ruminants

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 794-795
Author(s):  
D. Hernández-Sánchez ◽  
J. L. Gómez-Hernández ◽  
M. M. Crosby-Galván ◽  
A. M. Hernández-Anguiano ◽  
J. E. Ramirez-Bribiesca ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Lactobacillus Salivarius ◽  
Probiotic Potential

scholarly journals  Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro

2012 ◽  
Vol 57 (No. 11) ◽  
pp. 529-539 ◽  
Author(s):  
K. Saelim ◽  
N. Sohsomboon ◽  
S. Kaewsuwan ◽  
S. Maneerat
Keyword(s):  
Enterococcus Faecium ◽  
Probiotic Strain ◽  
Antagonistic Effect ◽  
Penicillin G ◽  
Probiotic Properties ◽  
Antibiotic Resistant ◽  
Good Tolerance ◽  
Probiotic Potential ◽  
Enterocin A

A bacteriocin-like substance (BLS) producing Enterococcus faecium CE5-1 was isolated from the gastrointestinal tract (GIT) of Thai indigenous chickens. Investigations of its probiotic potential were carried out. The competition between the BLS probiotic strain and antibiotic-resistant enterococci was also studied. Ent. faecium CE5-1 exhibited a good tolerance to pH 3.0 after 2 h and in 7% fresh chicken bile after 6 h, but the viability of Ent. faecium CE5-1 decreased by about 2–3 log CFU/ml after 2 h incubation in pH 2.5. It was susceptible to the antibiotics tested (tetracycline, erythromycin, penicillin G, and vancomycin). The maximum BLS production from Ent. faecium CE5-1 was observed at 15 h of cultivation. It showed activity against Listeria monocytogenes DMST17303, Pediococcus pentosaceus 3CE27, Lactobacillus sakei subsp. sakei JCM1157, and antibiotic-resistant enterococci. The detection by polymerase chain reaction (PCR) in the enterocin structural gene determined the presence of enterocin A gene in Ent. faecium CE5-1 only. Ent. faecium CE5-1 showed the highest inhibitory activity against two antibiotic-resistant Ent. faecalis VanB (from 6.68 to 4.29 log CFU/ml) and Ent. gallinarum VanC (from 6.76 to 4.31 log CFU/ml) after 12 h of co-cultivation. The results show the future possible use of Ent. faecium CE5-1 as a probiotic strain for livestock to control antibiotic-resistant enterococci.  

scholarly journals Efecto prebiótico de dos fuentes de inulina en el crecimiento in vitro de Lactobacillus salivarius y Enterococcus faecium

2018 ◽  
Vol 9 (2) ◽  
pp. 346 ◽  
Author(s):  
Marco Antonio Ayala Monter ◽  
David Hernández Sánchez ◽  
René Pinto Ruiz ◽  
Sergio S. González Muñoz ◽  
José Ricardo Bárcena Gama ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Lactobacillus Casei ◽  
Lactobacillus Salivarius

Las diarreas en especies pecuarias son controladas con antibióticos, pero su uso inadecuado causa problemas de resistencia bacteriana. Las bacterias ácido lácticas (BAL) en la microbiota intestinal ejercen exclusión competitiva contra patógenos causantes de diarreas, y la inulina es un sustrato para las BAL. Por lo tanto, el objetivo de este estudio fue determinar el efecto prebiótico de dos fuentes de inulina en el crecimiento in vitro de Lactobacillus salivarius (Ls) y Enterococcus faecium (Ef), con el uso de Lactobacillus casei (Lc) como control positivo. Las incubaciones in vitro se realizaron a 37 °C, con sustitución de glucosa por inulina de achicoria (IAc) o de agave (IAg) en el medio MRS. Los tratamientos (T) evaluados fueron T1: MRS-glucosa+Lc; T2: MRS-IAc+Lc, T3: MRS-IAc+Ls, T4: MRS-IAc+Ef, T5: MRS-IAg+Lc, T6: MRS-IAg+Ls y T7: MRS-IAg+Ef. La curva y la tasa de crecimiento se determinaron mediante densidad óptica (630 nm) a las 0, 3, 6, 12, 24, 30, 36, 48, 54 y 60 h. El diseño experimental fue completamente al azar, los datos se analizaron con PROC GLM (SAS) y la comparación de medias se realizó mediante la prueba de Tukey. Los tratamientos con MRS-IAg mostraron mayores (P<0.05) tasas de crecimiento (0.51a, 0.50a y 0.50a h-1, T5, T6 y T7, respectivamente) y resultaron similares al control positivo (T2) cuando creció en MRS-IAc (0.48a h-1). El pH durante el crecimiento fue diferente entre tratamientos (P<0.05). La inulina de agave favorece el crecimiento de bacterias probióticas como Lactobacillus salivarius y Enterococcus faecium, y su efecto prebiótico supera a la inulina de achicoria.

An enzyme-free hydrogen peroxide sensor for evaluation of probiotic potential of Enterococcus faecium

2018 ◽  
Vol 273 ◽  
pp. 298-304 ◽  
Author(s):  
Mourad Braik ◽  
Lucian-Gabriel Zamfir ◽  
Lucian Rotariu ◽  
Carmen Curutiu ◽  
Mariana Carmen Chifiriuc ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Enterococcus Faecium ◽  
Probiotic Potential ◽  
Hydrogen Peroxide Sensor ◽  
Free Hydrogen

Probiotic Potential and Immunomodulatory Properties in Enterococcus Faecium GMB24 and Enterococcus Hirae SMB16 Isolated from Goat and Sheep Milk

2021 ◽  
Author(s):  
Kamni Rajput ◽  
Ramesh Chandra Dubey ◽  
Ashwani Kumar Jangra
Keyword(s):  
Antibody Titer ◽  
Enterococcus Faecium ◽  
Delayed Type Hypersensitivity ◽  
Control Group ◽  
Neutrophil Adhesion ◽  
Bacterial Isolates ◽  
Enterococcus Hirae ◽  
Probiotic Potential ◽  
Sheep Milk ◽  
Antibiotic Susceptibility Test

Abstract Probiotic attributes of lactic acid bacteria isolated from goat and sheep milk samples were analysed by culturing them on MRS (de Man, Rogosa and Sharpe) agar media. The most potential isolates, GMB24 and SMB16, were identified by biochemical tests, which were further identified as Enterococcus faecium GMB24 and Enterococcus hirae SMB16 by 16S rRNA gene sequencing approach. The probiotic potential of the GMB24 and SMB16 were possessed for probiotics attributes including antimicrobial activity against five pathogenic bacteria viz., Escherichia coli (MTCC118), Staphylococcus aureus (MTCC7443), Pseudomonas aeruginosa (MTCC424), Listeria monocytogens (MTCC657) and Salmonella typhimurium (MTCC733), and antibiotic susceptibility test. These bacterial isolates had ability to tolerate different concentrations of acid, bile and phenol resistance. Further, immunomodulating activities of potential probiotic bacteria were tested on neutrophil adhesion test, haemagglutinating antibody titer and delayed type hypersensitivity. The data from these experiments were used for the principal component analysis (PCA) for assessing survivability of isolates at different factors. The probiotic bacterial isolates showed good response for the enhanced antibody production and delayed type hypersensitivity (DTH) activity. Probiotic isolates E. faecium GMB24 and E. hirae SMB16, at 109 cells/ml doses per day, increased the neutrophil adhesion, haemagglutinating antibody titer and DTH in comparison to the untreated control group. These differences were statistically significant (P < 0.05). The isolates showed negative test for hemolytic and gelatinase activities and hence were considered safe. E. faecium GMB24 and E. hirae SMB16 possessed an excellent probiotic potential and had a strong potential immune-stimulant activity.

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