Abstract
Probiotic attributes of lactic acid bacteria isolated from goat and sheep milk samples were analysed by culturing them on MRS (de Man, Rogosa and Sharpe) agar media. The most potential isolates, GMB24 and SMB16, were identified by biochemical tests, which were further identified as Enterococcus faecium GMB24 and Enterococcus hirae SMB16 by 16S rRNA gene sequencing approach. The probiotic potential of the GMB24 and SMB16 were possessed for probiotics attributes including antimicrobial activity against five pathogenic bacteria viz., Escherichia coli (MTCC118), Staphylococcus aureus (MTCC7443), Pseudomonas aeruginosa (MTCC424), Listeria monocytogens (MTCC657) and Salmonella typhimurium (MTCC733), and antibiotic susceptibility test. These bacterial isolates had ability to tolerate different concentrations of acid, bile and phenol resistance. Further, immunomodulating activities of potential probiotic bacteria were tested on neutrophil adhesion test, haemagglutinating antibody titer and delayed type hypersensitivity. The data from these experiments were used for the principal component analysis (PCA) for assessing survivability of isolates at different factors. The probiotic bacterial isolates showed good response for the enhanced antibody production and delayed type hypersensitivity (DTH) activity. Probiotic isolates E. faecium GMB24 and E. hirae SMB16, at 109 cells/ml doses per day, increased the neutrophil adhesion, haemagglutinating antibody titer and DTH in comparison to the untreated control group. These differences were statistically significant (P < 0.05). The isolates showed negative test for hemolytic and gelatinase activities and hence were considered safe. E. faecium GMB24 and E. hirae SMB16 possessed an excellent probiotic potential and had a strong potential immune-stimulant activity.
Probiotic Potential of Enterococcus faecium Isolated from Chicken Cecum with Immunomodulating Activity and Promoting Longevity in Caenorhabditis elegans