Multistage diagnosis of eye diseases using test systems

GlaucomaNews ◽  
2020 ◽  
pp. 48-49
Author(s):  
M.A. Kovalevskaya ◽  
◽  
L.A. Filina ◽  
Keyword(s):  

Главная причина обратимой слепоты – катаракта, которой страдают 33% людей с нарушением зрения. У пациентов с различными видами катаракт слезной жидкости определяют присутствие маркера возрастных изменений хрусталика – активного пероксиредоксина VI и/или фрагментов его распада. В случае наличия в слезной жидкости активного пероксиредоксина VI и/или фрагментов его распада диагностируют наличие возрастной катаракты. При отсутствии в слезной жидкости активного пероксиредоксина VI и/или фрагментов его распада диагностируют наличие катаракты, обусловленной изменениями хрусталика в ответ на фоновые расстройства метаболизма, катаракта при этом считается осложненной.

2009 ◽  
Author(s):  
Jing Guo ◽  
Louis Tay ◽  
Fritz Drasgow
Keyword(s):  

1979 ◽  
Author(s):  
Ronald M. Robertson ◽  
James W. Greene ◽  
Donald W. Maxwell ◽  
Carl E. Williams
Keyword(s):  

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S95-S112 ◽  
Author(s):  
A. H. W. M. Schuurs

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.


2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.


Author(s):  
Abdulrahman M. Ibrahem ◽  
Salah Q. Mahmood ◽  
Muhammed Babakir-Mina ◽  
Salar Ibrahim Ali ◽  
Bakhtyar Kamal Talabany

Knowledge and practice of public, especially patients about eye diseases are important to reduce magnitude of human blindness. Vision and sight are very essential because they allow us to connect to each other’s. In accordance to the recently published data; the estimation of 253 million people lives with vision impairment, 36 million are blind and 217 million suffer from moderate to severe vision impairment. A descriptive cross-sectional study was conducted at Shahid Dr. Aso Hospital in Sulaimani city-Iraq, from April to August 2017 by face-to-face interview through close ended questionnaire for data collecting. All data were analyzed by Statistical Package for Social Sciences version 22.0 software. P-value of < 0.05 was considered as a statistically significant. A total of 430 patients were randomly chosen to participate in the study. They were 254 (59.1%) males and 176 (40.9%) females. 76.7% of respondents was worrying about vision loss, 0.7% was worrying about hair loss. Of the participants, 32.8% was with a good knowledge level and 40.5% was with a poor knowledge level, as well as 3.1% was in a good practice and 58.8% was in a poor practice level. Female knowledge mean score was 9.53±4.96 and male knowledge mean score was 8.42±5.45, the practice mean score of males was 4.33±1.96 and mean practice score of females was 4.13±1.93. The study data indicate the worrying of participates about vision loss is in the highest proportion and the awareness and practice of patients about eye diseases is unsatisfactory. Health education campaigns are needed to improve personal awareness about vision related problems and for better eye health.


2018 ◽  
Author(s):  
V.G. Kopaeva ◽  
Keyword(s):  

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