Multifaceted bioproperties of Streptomyces bacillaris ANS2 isolated from Andaman and Nicobar Islands, India

2021 ◽  
Vol 16 (11) ◽  
pp. 99-108
Author(s):  
Kaari Manigundan ◽  
Joseph Jerrine ◽  
Manikkam Radhakrishnan ◽  
Anbalmani Sivarajan ◽  
Ramasamy Balagurunathan
Keyword(s):  
Larval Mortality ◽  
Radical Scavenging ◽  
Mangrove Sediment ◽  
Klebsiella Pneumonia ◽  
Cervical Cancer Cell Line ◽  
Rrna Gene ◽  
Andaman And Nicobar Islands ◽  
Marine Actinobacteria ◽  
Genus Streptomyces ◽  
Nicobar Islands

The rich warehouse called marine actinobacteria has enticed researchers for several decades. The assessment of their metabolites has evidenced several diverse and multiple biological properties vacillating from antibacterial, antituberculosis, antioxidant, anticancer to mosquitocidal. Therefore, the present study has been aimed to reconnoitre the isolation and portrayal of the promising marine actinobacteria named Streptomyces bacillaris ANS2 isolated from the mangrove sediment collected from Andaman and Nicobar islands. The ethyl acetate extract of ANS2 showed broad spectrum activity against various pathogens including Escherichia coli (18.0±0.3 mm), Salmonella paratyphi (16.0±0.6 mm) and Klebsiella pneumonia (14.8±0.7 mm). It showed maximum activity against drug sensitive strain of Mycobacterium tuberculosis (98.2±0.36%) followed by M. tuberculosis MDR strain (97.04±1.32%) and M. tuberculosis H37Rv (91.80±0.45%). The antioxidant activity by DPPH assay of ANS2 showed 29.9±0.38 - 61.35±0.78 % free radical scavenging at 100μg/ml - 500μg/ml as compared to the standard ascorbic acid which showed 96±1.0% at 100μg/ml concentration. The anticancer activity showed 88.12±0.9% and 89.5±0.26% inhibition against HT 29 (colon cancer) and HeLa (cervical cancer) cell line at 1000μg/ml concentration. The maximum larval mortality was recorded on Culex quinquefasciatus(LC50 =1100.134 μg/ml and r2= 0.99) and Aedes aegypti (LC50 =690.620 μg/ml and r2= 0.99).The biochemical, morphological and 16S rRNA gene analysis study clearly confirmed that the promising strain belonged to the genus Streptomyces bacillaris and the phylogenetic tree confirmed it as S. bacillaris. Results showed that S.bacillaris ANS2 showed multidimensional bioproperties.

Bacterial endosymbiont inhabiting Leucaena leucocephala leaves and their antioxidant and antidiabetic potential

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sridevi Chigurupati ◽  
Shantini Vijayabalan ◽  
Kesavanarayanan Krishnan Selvarajan ◽  
Ahmad Alhowail ◽  
Fatema Kauser
Keyword(s):  
Free Radical ◽  
Crude Extract ◽  
Leucaena Leucocephala ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
16S Rrna Gene Sequencing ◽  
Free Radical Scavenging ◽  
Rrna Gene ◽  
Standard Drug

AbstractObjectivesResearch on endosymbionts is emerging globally and is considered as a potential source of bioactive phytochemicals. The present study examines the antioxidant and antidiabetic of the endophytic crude extract isolated from Leucaena leucocephala leaves.MethodsEndophytic bacteria were isolated from the leaves of L. leucocephala and 16S rRNA gene sequencing was used to establish their identity. The in vitro antioxidant effect of endophytic crude extract (LL) was evaluated using 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) free radical scavenging methods. The in vitro antidiabetic properties of LL were evaluated using α-amylase and α-glucosidase enzyme inhibition assay.ResultsThe isolated endophytic bacteria were identified as Cronobacter sakazakii. LL displayed potent free radical scavenging effect against ABTS and DPPH radicals with an inhibitory concentration 50% (IC50) value of 17.49 ± 0.06 and 11.3 ± 0.1 μg/mL respectively. LL exhibited α-amylase and α-glucosidase inhibition with an IC50 value of 23.3 ± 0.08 and 23.4 ± 0.1 μg/mL respectively compared to the standard drug (acarbose). Both glucose loaded normoglycemic rats and STZ induced diabetic rats treated with LL (200 mg/kg) exhibited a considerable reduction in blood glucose levels p<0.01 after 8 h of treatment when compared to normal and diabetic control rats respectively.ConclusionsThus, the study shows that LL has a wellspring of natural source of antioxidants, and antidiabetic agents and phytoconstituents present in endophytes could be the rich source for bioactive compounds.

scholarly journals Composition and biological assays of the leaf essential oil of Asparagus flagellaris (Kunth) Bak

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Oluwakayode O. Odeja ◽  
Michael Gabriel Ibok ◽  
Ejike O. Okpala
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Klebsiella Pneumonia ◽  
Leaf Essential Oil

Abstract Background Asparagus flagellaris leaves are ethnomedicinally used to treat syphilis, gonorrhea and other sexually transmitted diseases (STDs), with no reports on the volatile constituents. This study was aimed to quantitatively and qualitatively characterise the composition of essential oil, evaluates the free radical scavenging and antimicrobial capacity of the essential oil. Methods The essential oil was isolated by hydrodistillation method using all-glass Clevenger-type apparatus, while the identification and quantification of constituents were performed by gas chromatography-mass spectrometry (GC-MS) technique. The antioxidant activity on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) was evaluated and the oil was also tested against 10 strains of microorganisms consisting of 6 bacteria: Escherichia coli, Salmonella typhi, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis and 4 fungi: Candida albicans, Penicillium notatum, Aspergillus niger and Rhizopus spp. using broth dilution and surface plate methods, respectively. Results A pale yellow essential oil with a characteristic scent was obtained, with a yield of 0.80% (w/w). A total of 28 compounds accounting for 97.41% of the total oil contents were identified. The oil was predominated by Thymol and its derivatives, accounting for 57.48%. The most abundance (% area) constituents of the essential oil were 5-Thymyl tiglate (18.49%), Thymyl-2-methyl butyrate (17.34%), Thymol hydroquinone dimethyl ether (10.52%), Thymol methyl ether (9.42%) and 5-Propyl-1, 3-benzodioxole (4.59%). The essential oil showed a significant free radical scavenging activity compared to the standard antioxidant drugs used in this study, with % inhibition varying from 88.06 ± 0.0001 to 93.05 ± 0.0006. The leaf essential oil exhibited antimicrobial activity on all the tested organisms at 500–125 μg/mL, with an 18–10 mm inhibitory zone. Conclusion The leaf essential oil of A. flagellaris contains notable chemical compounds responsible for its antioxidant and antimicrobial activities.

Predominance of hepatitis B virus genotype C among Karens, the ‘old settlers’ of Andaman and Nicobar Islands, India

2007 ◽  
Vol 152 (6) ◽  
pp. 1223-1228 ◽  
Author(s):  
S. Datta ◽  
P. K. Chandra ◽  
A. Banerjee ◽  
R. Chakravarty ◽  
K. M. Murhekar ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Andaman And Nicobar Islands ◽  
Virus Genotype ◽  
B Virus ◽  
Genotype C ◽  
Nicobar Islands

scholarly journals A Survey of Genetic Variation in Streptomyces Isolates Causing Potato Common Scab in the United States

2006 ◽  
Vol 96 (12) ◽  
pp. 1363-1371 ◽  
Author(s):  
Leslie A. Wanner
Keyword(s):  
United States ◽  
Genetic Variation ◽  
Common Scab ◽  
Disease Incidence ◽  
The United States ◽  
Rrna Gene ◽  
Population Genetic Study ◽  
Variable Regions ◽  
Genus Streptomyces ◽  
Characteristic Features

Common scab is a serious disease of potatoes and other root and tuber crops, affecting crop quality and market value. The disease is caused by gram positive soil bacteria in the genus Streptomyces. Disease incidence and severity vary in different locations and years; this is due in part to variation in the environment (weather) and genetic variation in potato cultivars. Little information is available on the contribution of genetic variation by the pathogen. To examine genetic diversity in different locations within the United States, streptomycetes were isolated from lesions on field-grown potatoes from six states. Isolates were classified into species based on sequence of variable regions in the 16s rRNA gene. The presence of genes associated with the recently described S. turgidiscabies pathogenicity island (PAI) was also determined. About half of the isolates belonged to S. scabies or S. europaeiscabiei based on 16s rDNA sequence, and had characteristic features of the PAI. They were found in all six states, and were pathogenic on potato and radish. The remaining isolates included pathogens and nonpathogens. They were varied in appearance, and represent several species, including one pathogenic species not previously reported. Some pathogenic isolates lacked one or more genes characteristic of the PAI, although all had genes for biosynthesis of the pathogenicity determinant thaxtomin. In this relatively small survey, regional differences in scab-causing streptomycetes were seen. This report furnishes tools and baseline data for population genetic study of scab-causing streptomycetes in the United States.

North-south gradient of incidence, distribution and variations of coral reef communities in the Andaman and Nicobar Islands, India

2017 ◽  
Vol 21 (2) ◽  
pp. 289-301 ◽  
Author(s):  
N. Marimuthu ◽  
J. S. Yogesh Kumar ◽  
C. Raghunathan ◽  
N. V. Vinithkumar ◽  
R. Kirubagaran ◽  
...  
Keyword(s):  
Coral Reef ◽  
Andaman And Nicobar Islands ◽  
Reef Communities ◽  
Nicobar Islands ◽  
Coral Reef Communities
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