Assessment of Pharmacodynamic Interactions of Vernonia Amygdalina –Metformin Co-Administration on Diabetic Wistar Rats.

Diabetes mellitus is prevalent in many countries of the world, affecting all ages both in developing and developed nations. The use of plants as remedies or preventive therapies has increased over the years. The study investigated the biochemical changes caused by combined leaf extracts of Vernonia amygdalina (bitter leaf) and Gnetum africanum (okazi leaf) on alloxan induced diabetic wistar rats.Aqueous extracts of the leaves were prepared using the conventional method.Forty male wistar rats weighing 150-180g were grouped into eight (five rats each). Group 1 was the normal control while diabetes was induced using alloxan (160mg/ kg)in groups 2-8.Group 2 received no treatment while groups 3-7 received varied ratios of the extracts at (BI/OK|10:90%), (BI/OK|30:70%), (BI/OK|50:50%), (BI/OK|70:30%) and (BI/OK|90:10%). Group 8 was the diabetic control treated with the standard diabetic drug (Metformin). The animals were weighed and blood glucose was determined at 7-day intervals. They were sacrificed on the 28th day and blood samples collected for serum protein, serum electrolyte, urea, creatinine, liver enzymes and markers of oxidative stress analyses. The results showed steady increase in the body weights (g) of the rats with (BI/OK|70:30)% treated group showing the highest increase (175.40±1.28). The fasting blood sugar (mg/dl) showed timedependent reduction in all the treated diabeti groups with (BI/OK|90:10)% having the highest (56.20±1.65) reduction. There was a significant increase (p<0.05) in total blood protein concentration (g/dl) in all the treated groups. The results of this study showed time and ratio dependent effect on the parameters measured. Since the two plants are staple vegetables in some countries, their utilization particularly in appropriate combinations should be encouraged. Key words: Extracts, bitter leaf, waterleaf, diabetes mellitus, biochemical parameters.

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Glucose Enzymatic Modulation by Vernonia amygdalina in Streptozotocin- Diabetic Wistar Rats

British Journal of Medicine and Medical Research ◽

10.9734/bjmmr/2016/24900 ◽

2016 ◽

Vol 15 (1) ◽

pp. 1-14

Author(s):

Anthony Ojieh ◽

Anthony Okolo ◽

Lawrence Ewhre ◽

Ikenna Njoku ◽

John Igweh ◽

...

Keyword(s):

Wistar Rats ◽

Vernonia Amygdalina

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An Evaluation of the Antioxidant and Blood Lead Lowering Effect of Vernonia Amygdalina Aqueous Extract on Lead Acetate Induced Oxidative Stress in Wistar Rats

American Journal of Biomedical Sciences ◽

10.5099/aj170400193 ◽

2017 ◽

pp. 193-199 ◽

Cited By ~ 1

Author(s):

Odewusi Odeyinka Olufunsho ◽

Tope-Ajayi Ayodele

Keyword(s):

Oxidative Stress ◽

Aqueous Extract ◽

Lead Acetate ◽

Wistar Rats ◽

Blood Lead ◽

Vernonia Amygdalina ◽

Lowering Effect

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Serum lipid and electrolyte profiles of Wistar rats fed with Vernonia amygdalina supplemented Vigna subterranea (Bambara groundnut) pudding

Calabar Journal of Health Sciences ◽

10.25259/cjhs_10_2019 ◽

2020 ◽

Vol 3 ◽

pp. 40-45

Author(s):

Godwin E. Egbung ◽

Nyakno A. Essien ◽

John E. Mgbang ◽

Josephine E. Egbung

Keyword(s):

Lipid Profile ◽

Normal Control ◽

Serum Lipid ◽

Wistar Rats ◽

Total Cholesterol Level ◽

Bambara Groundnut ◽

The Body ◽

Serum Lipid Profile ◽

Vernonia Amygdalina ◽

Group 2

Objective: This study investigated the effects of Vernonia amygdalina supplementation on Vigna subterrenea (bambara groundnut) pudding on serum lipid profile and electrolyte levels in Wistar rats. Materials and Methods: Twenty five weanling rats were randomly selected into 5 groups of 5 rats each. Group 1 received normal rat pellet. Groups 2 and 3 received 10% and 5% (w/w) non supplemented bambara pudding, respectively. Groups 4 and 5 were fed with 10% and 5% (w/w) Vernonia supplemented bambara pudding, respectively, for 21 days. The animals were sacrificed using anaesthesia at the end of the experiment and blood sample collected for serum lipid profile and serum electrolyte determinations using standard methods. Results: There was a significant increase (p<0.05) in total cholesterol level in groups 2 (1.64 ± 0.01 mmol/L) and 4 (1.66 ± 0.01 mmol/L) compared to normal control (1.31 ± 0.02 mmol/L). Triacylglycerol level increased significantly in groups 2, 3, and 4 compared to normal control. The high density lipoprotein cholesterol (HDL-c) and Serum sodium level were significantly increased (p < 0.05) in group 2 and 4 respectively compared to the normal control. Potassium, chloride, bicarbonate and calcium levels showed significant increases in group 2 relative to normal control. Phosphate level decreased significantly in group 2 (1.37 ± 0.03 mmol/L), 3 (1.43 ± 0.03 mmol/L) and 5 (1.37 ± 0.03 mmol/L) when compared to normal control (1.75 ± 0.03 mmol/L) respectively. Conclusion: Consumption of bambara groundnut pudding supplemented with Vernonia leaves increases HDL-c level as well as supply appreciable amounts of electrolytes in the body.

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