Fast Surface Immobilization of Native Proteins through Metal-Free Amino-yne Click Bioconjugation
Keyword(s):
In this work, we use a spontaneous and catalyst-free amino-yne click bioconjugation to generate activated ethynyl group functionalized surfaces for fast immobilizing native proteins and cells. Biomolecules, such as bovine serum albumin (BSA), human IgG and peptide of C(RGDfK), could be covalently immobilized on the surfaces in as short as 30 min. Notably, the bioactivity of the anchored biomolecules remain intact, which is verified by efficiently capturing target antibodies and cells from the bulk solutions. This strategy represents an alternative for highly efficient surface biofunctionalization.
2019 ◽
Keyword(s):
2017 ◽
Vol 15
(7)
◽
pp. 1549-1553
◽
1995 ◽
Vol 29
(6)
◽
pp. 707-714
◽
Keyword(s):
1987 ◽
Vol 45
◽
pp. 762-763
Keyword(s):
1984 ◽
Vol 98
(1)
◽
pp. 138-143
◽
Keyword(s):
1981 ◽
Vol 46
(03)
◽
pp. 645-647
◽