scholarly journals Towards Large-Scale Steady-State Enhanced Nuclear Magnetization with In Situ Detection

2021 ◽  
Author(s):  
John Blanchard ◽  
Barbara Ripka ◽  
Benjamin A. Suslick ◽  
Dario Gelevski ◽  
Teng Wu ◽  
...  
Keyword(s):  
Large Scale ◽  
Zero Field ◽  
Fundamental Physics ◽  
Solvent Vapor ◽  
Chemically Modified ◽  
Nuclear Magnetization ◽  
In Situ Detection ◽  
New Applications ◽  
Nmr Signals

<div>Signal Amplification By Reversible Exchange (SABRE) boosts NMR signals of various nuclei enabling new applications spanning from magnetic resonance imaging to analytical chemistry and fundamental physics. SABRE is especially well positioned for continuous generation of enhanced magnetization on a large scale, however, several challenges need to be addressed for accomplishing this goal. Specifically, SABRE requires (i) a specialized catalyst capable of reversible H<sub>2</sub> activation and (ii) physical transfer of the sample from the point of magnetization generation to the point of detection (e.g., a high-field or a benchtop NMR spectrometer). Moreover, (iii) continuous parahydrogen bubbling accelerates solvent (e.g., methanol) evaporation, thereby limiting the experimental window to tens of minutes per sample.</div><div>In this work, we demonstrate a strategy to rapidly generate the best-to-date precatalyst (a compound that is chemically modified in the course of the reaction to yield the catalyst) for SABRE, [Ir(IMes)(COD)Cl] (IMes = 1,3-bis-(2,4,6-trimethylphenyl)-imidazol-2-ylidene, COD = cyclooctadiene) via a highly accessible synthesis. Second, we measure hyperpolarized samples using a home-built zero-field NMR spectrometer and study the field dependence of hyperpolarization directly in the detection apparatus, eliminating the need to physically move the sample during the experiment. Finally, we prolong the measurement time and reduce evaporation by presaturating parahydrogen with the solvent vapor before bubbling into the sample. These advancements extend opportunities for exploring SABRE hyperpolarization by researchers from various fields and pave the way to producing large quantities of hyperpolarized material for long-lasting detection of SABRE-derived nuclear magnetization.</div>

scholarly journals Towards Large-Scale Steady-State Enhanced Nuclear Magnetization with In Situ Detection

2021 ◽  
Author(s):  
John Blanchard ◽  
Barbara Ripka ◽  
Benjamin A. Suslick ◽  
Dario Gelevski ◽  
Teng Wu ◽  
...  
Keyword(s):  
Large Scale ◽  
Zero Field ◽  
Fundamental Physics ◽  
Solvent Vapor ◽  
Chemically Modified ◽  
Nuclear Magnetization ◽  
In Situ Detection ◽  
New Applications ◽  
Nmr Signals

<div>Signal Amplification By Reversible Exchange (SABRE) boosts NMR signals of various nuclei enabling new applications spanning from magnetic resonance imaging to analytical chemistry and fundamental physics. SABRE is especially well positioned for continuous generation of enhanced magnetization on a large scale, however, several challenges need to be addressed for accomplishing this goal. Specifically, SABRE requires (i) a specialized catalyst capable of reversible H<sub>2</sub> activation and (ii) physical transfer of the sample from the point of magnetization generation to the point of detection (e.g., a high-field or a benchtop NMR spectrometer). Moreover, (iii) continuous parahydrogen bubbling accelerates solvent (e.g., methanol) evaporation, thereby limiting the experimental window to tens of minutes per sample.</div><div>In this work, we demonstrate a strategy to rapidly generate the best-to-date precatalyst (a compound that is chemically modified in the course of the reaction to yield the catalyst) for SABRE, [Ir(IMes)(COD)Cl] (IMes = 1,3-bis-(2,4,6-trimethylphenyl)-imidazol-2-ylidene, COD = cyclooctadiene) via a highly accessible synthesis. Second, we measure hyperpolarized samples using a home-built zero-field NMR spectrometer and study the field dependence of hyperpolarization directly in the detection apparatus, eliminating the need to physically move the sample during the experiment. Finally, we prolong the measurement time and reduce evaporation by presaturating parahydrogen with the solvent vapor before bubbling into the sample. These advancements extend opportunities for exploring SABRE hyperpolarization by researchers from various fields and pave the way to producing large quantities of hyperpolarized material for long-lasting detection of SABRE-derived nuclear magnetization.</div>

scholarly journals Towards Large‐Scale Steady‐State Enhanced Nuclear Magnetization with In Situ Detection

2021 ◽  
Author(s):  
John W. Blanchard ◽  
Barbara Ripka ◽  
Benjamin A. Suslick ◽  
Dario Gelevski ◽  
Teng Wu ◽  
...  
Keyword(s):  
Steady State ◽  
Large Scale ◽  
Nuclear Magnetization ◽  
In Situ Detection

scholarly journals Facile Preparation of Self-Assembled Polydopamine-Modified Electrospun Fibers for Highly Effective Removal of Organic Dyes

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 116 ◽  
Author(s):  
Cuiru Wang ◽  
Juanjuan Yin ◽  
Ran Wang ◽  
Tifeng Jiao ◽  
Haiming Huang ◽  
...  
Keyword(s):  
Active Sites ◽  
Large Scale ◽  
Organic Dyes ◽  
Electrospun Fiber ◽  
Electrospun Fibers ◽  
Wastewater Purification ◽  
Solvent Vapor ◽  
Effective Removal ◽  
Microscopic Morphology

Polydopamine (PDA) nanoparticles can be used as an adsorbent with excellent adsorption capacity. However, nanosized adsorbents are prone to aggregation and thus are severely limited in the field of adsorption. In order to solve this problem, we utilized polydopamine in-situ oxidation self-polymerization on the surface of polycaprolactone (PCL)/polyethylene oxide (PEO) electrospun fiber after solvent vapor annealing (SVA) treatment, and successfully designed and prepared a PCL/PEO@PDA composite membrane. The SVA treatment regulated the microscopic morphology of smooth PCL/PEO electrospun fibers that exhibited a pleated microstructure, increasing the specific surface area, and providing abundant active sites for the anchoring of PDA nanoparticles. The PCL/PEO@PDA composite obtained by chemical modification of PDA demonstrated numerous active sites for the adsorption of methylene (MB) and methyl orange (MO). In addition, the PCL/PEO@PDA composites were reusable several times with good reutilization as adsorbents. Therefore, we have developed a highly efficient and non-agglomerated dye adsorbent that exhibits potential large-scale application in dye removal and wastewater purification.

Size Effects in Plasticity: Experiments and Simulations

2006 ◽  
Vol 503-504 ◽  
pp. 193-200 ◽  
Author(s):  
Helena Van Swygenhoven
Keyword(s):  
Large Scale ◽  
Tensile Deformation ◽  
Profile Analysis ◽  
Bragg Diffraction ◽  
X Ray Diffraction ◽  
X Ray ◽  
Diffraction Peaks ◽  
New Applications ◽  
Source And Sink

Large scale computer simulations suggest that in nanocrystalline metals grain boundaries act as source and sink for dislocations. This suggestion has been the motivation for developing a new in-situ X-ray diffraction technique that allow peak profile analysis of several Bragg diffraction peaks during tensile deformation. Synergies between simulations and experiments are discussed including new applications of the in-situ technique.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

scholarly journals IMPROVING SYSTEM READINESS, STAFF PREPAREDNESS AND PATIENT SAFETY IN A NEW SINGLE FAMILY ROOM NICU THROUGH IN SITU SIMULATION

2018 ◽  
Vol 23 (suppl_1) ◽  
pp. e16-e16
Author(s):  
Ahmed Moussa ◽  
Audrey Larone-Juneau ◽  
Laura Fazilleau ◽  
Marie-Eve Rochon ◽  
Justine Giroux ◽  
...  
Keyword(s):  
Patient Safety ◽  
Repeated Measures ◽  
Large Scale ◽  
Multidisciplinary Teams ◽  
Single Family ◽  
In Situ Simulation ◽  
Ensure Patient Safety ◽  
System Readiness ◽  
Dependent Samples

Abstract BACKGROUND Transitions to new healthcare environments can negatively impact patient care and threaten patient safety. Immersive in situ simulation conducted in newly constructed single family room (SFR) Neonatal Intensive Care Units (NICUs) prior to occupancy, has been shown to be effective in testing new environments and identifying latent safety threats (LSTs). These simulations overlay human factors to identify LSTs as new and existing process and systems are implemented in the new environment OBJECTIVES We aimed to demonstrate that large-scale, immersive, in situ simulation prior to the transition to a new SFR NICU improves: 1) systems readiness, 2) staff preparedness, 3) patient safety, 4) staff comfort with simulation, and 5) staff attitude towards culture change. DESIGN/METHODS Multidisciplinary teams of neonatal healthcare providers (HCP) and parents of former NICU patients participated in large-scale, immersive in-situ simulations conducted in the new NICU prior to occupancy. One eighth of the NICU was outfitted with equipment and mannequins and staff performed in their native roles. Multidisciplinary debriefings, which included parents, were conducted immediately after simulations to identify LSTs. Through an iterative process issues were resolved and additional simulations conducted. Debriefings were documented and debriefing transcripts transcribed and LSTs classified using qualitative methods. To assess systems readiness and staff preparedness for transition into the new NICU, HCPs completed surveys prior to transition, post-simulation and post-transition. Systems readiness and staff preparedness were rated on a 5-point Likert scale. Average survey responses were analyzed using dependent samples t-tests and repeated measures ANOVAs. RESULTS One hundred eight HCPs and 24 parents participated in six half-day simulation sessions. A total of 75 LSTs were identified and were categorized into eight themes: 1) work organization, 2) orientation and parent wayfinding, 3) communication devices/systems, 4) nursing and resuscitation equipment, 5) ergonomics, 6) parent comfort; 7) work processes, and 8) interdepartmental interactions. Prior to the transition to the new NICU, 76% of the LSTs were resolved. Survey response rate was 31%, 16%, 7% for baseline, post-simulation and post-move surveys, respectively. System readiness at baseline was 1.3/5,. Post-simulation systems readiness was 3.5/5 (p = 0.0001) and post-transition was 3.9/5 (p = 0.02). Staff preparedness at baseline was 1.4/5. Staff preparedness post-simulation was 3.3/5 (p = 0.006) and post-transition was 3.9/5 (p = 0.03). CONCLUSION Large-scale, immersive in situ simulation is a feasible and effective methodology for identifying LSTs, improving systems readiness and staff preparedness in a new SFR NICU prior to occupancy. However, to optimize patient safety, identified LSTs must be mitigated prior to occupancy. Coordinating large-scale simulations is worth the time and cost investment necessary to optimize systems and ensure patient safety prior to transition to a new SFR NICU.

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

Sign in / Sign up

Export Citation Format

Share Document