Enhancing The Photocatalytic Conversion Of Pt(IV) Substrates By Flavoprotein Engineering
Keyword(s):
Our recent work demonstrates that certain flavoproteins can catalyze the redox activation of Pt(IV) prodrug complexes under light irradiation. Herein, we used site directed mutagenesis on the mini Singlet Oxygen Generator (mSOG) to modulate the photocatalytic activity of this flavoprotein towards two model Pt(IV) substrates. Among the prepared mutants, Q103V mSOG displayed enhanced catalytic efficiency as a result of its longer triplet excited state lifetime. This study shows, for the first time, that protein engineering can improve the catalytic capacity of a protein towards metal-containing substrate.
2021 ◽
2019 ◽
Vol 190
(4)
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pp. 1257-1270
2012 ◽
Vol 78
(11)
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pp. 3880-3884
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2005 ◽
Vol 187
(21)
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pp. 7543-7545
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2018 ◽
2021 ◽
pp. 148505
Kinetic Studies on CphA Mutants Reveal the Role of the P158-P172 Loop in Activity versus Carbapenems
2016 ◽
Vol 60
(5)
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pp. 3123-3126
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Keyword(s):
Zinc Ion
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2016 ◽
Vol 64
(17)
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pp. 3386-3393
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Keyword(s):