Identification of Components In Boerhavia Diffusa Plant Extract And Assessment Of Its Biological Activity By Gas Chromatography-Mass spectrometry

Boerhavia diffusa is a medicinal plant used for various therapeutic benefits ant the plant can be found in tropics and subtropics regions. It has the pharmacological activities which include antifibrinolytic, immunosuppressive, diuretic, hepatoprotective, antidiabetic, anti-inflammatory, anticancer, immunomodulatory, antilympho proliferative, analgesic properties and also for the treatment of pulmonary tuberculosis. This study deals with the determination of possible phytocompounds present in the hydroethanolic extract of the plant Boerhavia diffusa using Gas chromatography- Mass Spectroscopy technique. Using GC-MS technique the phytocompounds present in the hydroethanolic extract of the plant Boerhavia diffusa was investigated and the mass spectra of the compounds found in the extract were matched with National Institute of Standards and Technology library. GC-MS Analysis of Boerhavia diffusa extract reveals that the existence of phytocompounds which includes 1, 2-Benzenedicarboxylic acid, diethyl ester, Hexadecanoic Acid, 1,2- Benzenedicarboxylic acid, dibutyl ester and 1,2-Benzenedicarboxylic Acid, Dioctyl Ester. This research paper helps to identify the phytocompounds which are present in the extract of Boerhavia diffusa and also prove the biological activities of the phytocompounds present. Furthermore, studies were made in-depth to find the mode of action of the phytocompounds.

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In Vitro Antifunal Potential of Morganella morganii and Determination of its Chemical Composition by Gas Chromatography-Mass Spectrometry

International Journal of Current Pharmaceutical Review and Research ◽

10.25258/ijcprr.v8i02.9193 ◽

2017 ◽

Vol 8 (02) ◽

Author(s):

Sabreen A. Kamal

Keyword(s):

Gas Chromatography ◽

Biological Activities ◽

Gas Chromatography Mass Spectrometry ◽

Morganella Morganii ◽

Highly Active ◽

Gas Chromatography Mass Spectroscopy ◽

Antibacterial And Antifungal ◽

Anti Fungal

Bioactives were analyzed using gas chromatography-mass spectroscopy (GC-MS) techniques, then the in vitro antibacterial and antifungal activity of the methanolic extract was evaluated. GC-MS analysis of Morganella morganii revealed the existence of the Tricyclo[4.3.1.1(3.8)]undecan-1-amine, 3-Methoxybenzaldehyde semicarbazone, carboxaldehyde , 1-methyl-,oxime ,(Z)-(+), 1,5,5-Trimethyl-6-methylene-cyclohexene, 4-(2,5-Dihydro-3-methoxyphenyl)butylamine, Paromomycin , 9-Borabicyclo[3.31]nonane , 9-mercapto-, Benzenemethanol , 2-(2-aminopropoxy)-3-methyl, Acetamide , N-(6-acetylaminobenzothiazol-2-yl)-2-(adamantan, rin-6-carboxylic acid , 4-(2,5-Dihydro-3-methoxyphenyl)butylamine, N-(2,5-Dicyano-3,4-dihydro-2H-pyrrol-2-yl)-acetamide, 3,10-Dioxatricyclo [4.3.1.0(2,4)]dec-7-ene, 3-Cyclohex-3-enyl-propionic acid, Eicosanoic acid ,phenylmethyl ester, 3,7-Diazabicyclo[3.3.1]nonane , 9,9-dimethyl-, Dithiocarbamate , S-methyl-,N-(2-methyl-3-oxobutyl)-, dl-Homocysteine, 2-(2-Furyl)pyridine, 1,7-Dioxa-10-thia-4,13-diazacyclopentadeca-5,9,12-trione, 5,7-Dodecadiyn-1,12-diol, 1-(β-d-Arabinofuranosyl)-4-O-difluoromethyluracil, Uric acid, Pyrrolo[1.2-a]pyrazine-1,4-dione , hexahydro-,12-Methyl-oxa-cyclododecan-2-one, Phthalic acid , butyl undecyl ester, 9,12,15-Octadecatrienoic acid , 2,3-bis(acetyloxy)propyl ester, 1,2,4-Trioxolane-2-octanoic acid 5-octyl-, methyl ester, 12-Dimethylamino-10-oxododecanoic acid , Octahydrochromen-2-one, L-Aspartic acid , N-glycyl-,2H-Oxecin-2-one , 3,4,7,8,91,10-hexahydro-4-hydroxy-10-meth , Thiazolo[4,5-d]pyrimidine-5,7(4H,6H)-dione , 2-amino-4-(2-ph, Dec-9-en-6-oxo-1-ylamide, 3,6,12-Trimethyl-1,4,7,10,13,16-hexaaza-cyclooctadecane, 2-lodohiistidine, 2,5-Piperazinedione ,3,6-bis(2-methylpropyl)-, 9-Octadecenamide , (Z)-, 3',8,8'-Trimethoxy-3-piperidyl-2,2'-binaphthalene-1,1',4,4'-tetra. Citrullus colocynthis (Crude) was very highly active (6.39±0.27) mm. The results of anti-fungal activity produced by Morganella morganii showed that the volatile compounds were highly effective to suppress the growth of Aspergillus terreus (5.613±0.23). Morganella morganii produce many important secondary metabolites with high biological activities. Based on the significance of employing bioactive compounds in pharmacy to produce drugs for the treatment of many diseases, the purification of compounds produced by Morganella morganii can be useful.

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IDENTIFICATION OF BIOACTIVE COMPONENTS IN ENHALUS ACOROIDES SEAGRASS EXTRACT BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i10.25577 ◽

2018 ◽

Vol 11 (10) ◽

pp. 313 ◽

Cited By ~ 1

Author(s):

Amudha P ◽

Jayalakshmi M ◽

Pushpabharathi N ◽

Vanitha V

Keyword(s):

Gas Chromatography ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antioxidant Property ◽

Butylated Hydroxytoluene ◽

Gas Chromatography Mass Spectrometry ◽

Antitumor Activities ◽

Enhalus Acoroides ◽

Gas Chromatography Mass Spectroscopy

Objective: This study deals with the determination of possible phytocompounds present in the ethyl acetate extract of Enhalus acoroides using gas chromatography-mass spectroscopy (GC-MS) technique. Methods: Using GC-MS technique the phytocompounds present in the ethyl acetate extract of E. acoroides whole seagrass was investigated, and the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology library.Results: GC-MS analysis of E. acoroides extract revealed the existence of several phytocompounds which includes 1-nonadecene (17.15%), n-tetracosanol-1 (11.48%), 1-octadecene (10.06%), 2-pentadecanone (7.87%), behenyl alcohol (7.33%), 17-pentatriacontene (4.84%), triacontane (4.25%), tetratetracontane (4.17%), and butylated hydroxytoluene (2.09%).Conclusion: E. acoroides possess distinct phytocompounds such as 1-nonadecene and n-tetracosanol-1 which possess antioxidant property, triacontane which has antibacterial, antidiabetic and antitumor activities. Further studies need to elute novel bioactive compounds and toxicity profile through animal models.

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GAS CHROMATOGRAPHY MASS SPECTROSCOPY ANALYSIS AND PREDICTION OF BIOACTIVITIES IN THE CHLOROFORM EXTRACT OF HALYMENIA DILATATA ZANARDINI (RED ALGAE) COLLECTED FROM MANDAPAM, TAMIL NADU, INDIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i12.43068 ◽

2021 ◽

pp. 60-63

Author(s):

FREDRICK RAJA E ◽

JOHN PETER PAUL J

Keyword(s):

Gas Chromatography ◽

Mass Spectroscopy ◽

Red Algae ◽

Tamil Nadu ◽

Biological Activities ◽

Chloroform Extract ◽

Bioactive Components ◽

Benzenedicarboxylic Acid ◽

Anti Inflammatory ◽

Gas Chromatography Mass Spectroscopy

Objective: This study was to determine the presence of biochemicals and prediction of bioactivities in the chloroform extract of Halymenia dilatata Zanardini (Red algae) collected from Mandapam, Tamil Nadu, India. Methods: The active biological components in the chloroform extract of H. dilatata Zanardini were studied using gas chromatography mass spectroscopy (GC-MS), and the biological activities were predicted by prediction activity spectra for substances technique. Results: The analysis revealed the two bioactive components such as N-Hexadecanoic acid (5.384%) and 1,2-Benzenedicarboxylic acid, Mono(2- Ethylhexyl) Ester (94.616%). In 1,2-Benzenedicarboxylic acid, Mono(2-Ethylhexyl) Ester, 1933 biological activities were predicted. Among the biological activities, 74 were highly active and their Pa score is above 70%. In Pa>0.7, there a 15 different activities are predicated including antitumor (91.5%) followed by anti-inflammatory (89.7%), antimicrobial (84.7%), anti-inflammatory (74.5%), etc. Conclusion: The present study provided the bioactive components present in the chloroform extract of H. dilatata Zanardini by GC-MS analysis and the prediction of biological studies by prediction of activity spectra for substances.

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