scholarly journals GAS CHROMATOGRAPHY MASS SPECTROSCOPY ANALYSIS AND PREDICTION OF BIOACTIVITIES IN THE CHLOROFORM EXTRACT OF HALYMENIA DILATATA ZANARDINI (RED ALGAE) COLLECTED FROM MANDAPAM, TAMIL NADU, INDIA

Author(s):  
FREDRICK RAJA E ◽  
JOHN PETER PAUL J

Objective: This study was to determine the presence of biochemicals and prediction of bioactivities in the chloroform extract of Halymenia dilatata Zanardini (Red algae) collected from Mandapam, Tamil Nadu, India. Methods: The active biological components in the chloroform extract of H. dilatata Zanardini were studied using gas chromatography mass spectroscopy (GC-MS), and the biological activities were predicted by prediction activity spectra for substances technique. Results: The analysis revealed the two bioactive components such as N-Hexadecanoic acid (5.384%) and 1,2-Benzenedicarboxylic acid, Mono(2- Ethylhexyl) Ester (94.616%). In 1,2-Benzenedicarboxylic acid, Mono(2-Ethylhexyl) Ester, 1933 biological activities were predicted. Among the biological activities, 74 were highly active and their Pa score is above 70%. In Pa>0.7, there a 15 different activities are predicated including antitumor (91.5%) followed by anti-inflammatory (89.7%), antimicrobial (84.7%), anti-inflammatory (74.5%), etc. Conclusion: The present study provided the bioactive components present in the chloroform extract of H. dilatata Zanardini by GC-MS analysis and the prediction of biological studies by prediction of activity spectra for substances.

2020 ◽  
pp. 134
Author(s):  
Ira Rahmiyani

Minyak atsiri merupakan salah satu kelompok senyawa metabolit sekunder yang memiliki aroma khas dan mudah menguap. Daun gamal menghasilkan minyak atsiri yang dapat digunakan sebagai anti bakteri dan anti skabies. Tujuan penelitian ini untuk mengetahui komponen penyusun senyawa minyak atsiri dalam daun gamal. Metode yang digunakan untuk mengambil minyak atsiri dari daun gamal adalah destilasi uap air. Minyak atsiri yang dihasilkan dianalisis menggunakan Gas Chromatography Mass Spectroscopy (GC-MS). Hasil penelitian menunjukkan bahwa minyak atsiri daun gamal mengandung  100 komponen senyawa minyak atsiri dan 3 komponen senyawa utama minyak atsiri yaitu: 2-Pentadecanone, 6,10,14-trimethyl- (CAS) 6,10,14-Trimethyl-2-pentadecanone 20,07%, 2-Hexadecen-1-ol, 3,7,11,15-tetramethyl-, [R-[R*,R*-(E)]]- (CAS) Phytol 13,55%, 1,2-Benzenedicarboxylic acid, bis(2-ethylhexyl) ester (CAS)-bis(2-ethylhexyl)phthalate 10,11%. Kata kunci: Daun gamal, Destilasi Uap Air , GC-MS, Minyak atsiri


2020 ◽  
Vol 11 (3) ◽  
pp. 3590-3594
Author(s):  
Murugan K ◽  
Kalaivani P ◽  
Bothiraj K V ◽  
Vanitha V

Boerhavia diffusa is a medicinal plant used for various therapeutic benefits ant the plant can be found in tropics and subtropics regions. It has the pharmacological activities which include antifibrinolytic, immunosuppressive, diuretic, hepatoprotective, antidiabetic, anti-inflammatory, anticancer, immunomodulatory, antilympho proliferative, analgesic properties and also for the treatment of pulmonary tuberculosis. This study deals with the determination of possible phytocompounds present in the hydroethanolic extract of the plant Boerhavia diffusa using Gas chromatography- Mass Spectroscopy technique. Using GC-MS technique the phytocompounds present in the hydroethanolic extract of the plant Boerhavia diffusa was investigated and the mass spectra of the compounds found in the extract were matched with National Institute of Standards and Technology library. GC-MS Analysis of Boerhavia diffusa extract reveals that the existence of phytocompounds which includes 1, 2-Benzenedicarboxylic acid, diethyl ester, Hexadecanoic Acid, 1,2- Benzenedicarboxylic acid, dibutyl ester and 1,2-Benzenedicarboxylic Acid, Dioctyl Ester. This research paper helps to identify the phytocompounds which are present in the extract of Boerhavia diffusa and also prove the biological activities of the phytocompounds present. Furthermore, studies were made in-depth to find the mode of action of the phytocompounds.


Author(s):  
Jayalakshmi M ◽  
Vanitha V ◽  
Sangeetha R

Objectives: Bioactive components determined by plants are known to have a broad application in the medical field. The focus of this study is to recognize the phytochemicals in the ethanol extract of Brassica oleracea by gas chromatography–mass spectroscopy (GC–MS). Methods: B. oleracea was collected, dried, and powdered well. The extraction was done with the solvent ethanol. The extract was exposed to column of GC-MS-QP 2010 (SHIMADZU) column Db 30.0 (0.25 μm in diameter, 0.25 μm thick). Results: GC–MS result provides the chromatogram with different peaks obtained at a different retention time shows the presence of various biocompounds. Some of the identified bioactive compounds are n-hexadecanoic acid (12.99%), phytol (2.40%), Vitamin E (3.38%), tetratetracontane (2.15%), stigmasterol (2.03%), and isophytol. Conclusion: The GC–MS study of the ethanol extract of B. oleracea reveals the existence of many potential compounds that can be utilized in the pharmaceutical industry, including the use of anti-inflammatory, antiarthritic, anticoronary, and antidiabetic agents.


2009 ◽  
Vol 1 (2) ◽  
pp. 387-392 ◽  
Author(s):  
M. N. I. Bhuiyan ◽  
J. Begum ◽  
P. K. Sardar ◽  
M. S. Rahman

The chemical constituents of leaf and peel essential oil of Citrus medica L. were analysed by gas chromatography mass spectroscopy (GC-MS). Nineteen components accounting for 99.9% of the oil were identified in leaf oil. The major constituents are erucylamide (28.43%), limonene (18.36%) and citral (12.95%). The peel oil contains forty three components accounting for 99.8% of the total oil and the major components are isolimonene (39.37%), citral (23.12%) and limonene (21.78%). Keywords: Citrus medica; Essential oils; GC-MS; Erucylamide; Isolimonene. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.DOI: 10.3329/jsr.v1i2.1760   


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