scholarly journals Pharmacognostical standardization, phytochemical analysis, and antioxidant activity of Musa balbisiana Colla fruits

2020 ◽  
Vol 11 (4) ◽  
pp. 7920-7931
Author(s):  
Ly Hai Trieu ◽  
Le My Huyen ◽  
Lam Bich Thao ◽  
Le Duc Thanh ◽  
Pham Thanh Huyen ◽  
...  
Keyword(s):  
Crude Extract ◽  
Raw Materials ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Antioxidant Property ◽  
Phytochemical Analysis ◽  
Musa Balbisiana

Musa balbisiana (MB) fruits are widespread as an herbal medicine worldwide.  This study aimed to screen phytochemicals, standardize raw materials and crude extract, and evaluate antioxidant property of MB fruit extracts. Standardization was performed using purity content and chemical characterizations, total polyphenol (TPC) and flavonoid (TFC) contents to assess the quality. The phytochemicals were determined by chemical reactions. DPPH quenching, ABTS cation decolorization, and reducing power assays were selected to investigate antioxidant ability. Results indicated that physicochemical parameters for MB fruits were within prescribed Vietnamese Pharmacopoeia 5th limits.  Phytochemical analysis of MB fruits disclosed the presence of volatile oils, lipids, flavonoids, proanthocyanidins, saponins, and triterpenoid. The TPC was 3.92 ± 0.27 mg GAE/g d. w. (dry weight) and 162.64 ± 3.39 mg GAE/g d. w. for the raw materials and crude extract respectively while the TFC was 0.138 ± 0.002 mg QE/g d. w. and 1.222 ± 0.007 mg QE/g d. w. Furthermore, crude extract and some of its fractions have a high radical scavenging activity. These initial studies play a pivotal role in the procurement of raw materials and evaluation of the quality of the crude extract, and show antioxidant potential of MB fruits. 

scholarly journals Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Vijayakumar Sundaram ◽  
Selvaraju Sadhasivam ◽  
Sivaraj Chandrasekaran ◽  
Raaman Nanjian ◽  
Arjun Pandian
Keyword(s):  
Dermal Fibroblast ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Fibroblast Cell ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Scavenging Activity ◽  
Medicinal Uses

Abstract Background Strobilanthes heyneanus (Nilgirianthus heyneanus) belongs to the family Acanthaceae that contains many species with potential for diverse medicinal uses. It is also called ‘Karun kurinji’ and is commonly found in the South-West regions of India. The species are commonly used in rheumatic complaints, sprain of the ankle, and hernia. The objectives of the study were to evaluate the antioxidant activity, phytochemical analysis, and antibacterial activities of the root extract of S. heyneanus. Results The radical scavenging and reduction assays such as DPPH● radical and OH● radical scavenging assays, as well as phosphomolybdenum reduction and Fe3+ reducing power assays, were determined for the root extract. The highest DPPH● radical scavenging activity was 88.23 ± 1.32 at 120 μg/mL concentration, and the calculated IC50 was 38.52 μg/mL concentration. The highest OH● radical scavenging activity was 51.28 ± 1.06 at 120 μg/mL concentration, and the calculated IC50 was 51.28 μg/mL concentration. The highest ABTS●+ radical scavenging activity was 91.28 ± 1.12 at 30 μg/mL concentration, and the calculated IC50 was 33.92 μg/mL concentration. The highest phosphomolybdenum reduction was 87.43 ± 0.90 at 120 μg/mL concentration, and the calculated RC50 was 24.74 μg/mL concentration. The highest Fe3+ reduction was 89.38 ± 0.98 at 120 μg/mL concentration, and the calculated RC50 was 31.06 μg/mL concentration. The antibacterial activity of S. heyneanus showed the highest zone of inhibition of 24 mm for Salmonella typhi with 500 μg/mL concentration. The cytotoxicity limits of the root extracts were tested by MTT assay using human dermal fibroblast cell lines, reflecting > 90% cell viability at a concentration of 500 μg/mL. Conclusions The current study showed that the root extract of S. heyneanus has better antioxidant properties and potential anti-bacterial compounds. The phytochemical analysis of the root extract showed the presence of alkaloids, steroids, terpenoids, phenols, flavonoids, tannins, saponins, glycosides, and carbohydrates, which are responsible for the antibacterial root extract of S. heyneanus synergistically.

scholarly journals The quality of carrot (Daucus carota L.) cultivated in the field depending on iodine and selenium fertilization

2016 ◽  
Vol 28 (2) ◽  
pp. 151-164 ◽  
Author(s):  
Sylwester Smoleń ◽  
Łukasz Skoczylas ◽  
Iwona Ledwożyw-Smoleń ◽  
Roksana Rakoczy ◽  
Marta Liszka-Skoczylas ◽  
...  
Keyword(s):  
Daucus Carota ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Root Weight ◽  
Storage Roots ◽  
Selenium Compounds ◽  
Daucus Carota L ◽  
Selenium Fertilization

AbstractThe aim of the study was to evaluate the influence of various chemical forms of iodine (I−and IO3−) and selenium (SeO32−and SeO42−) on the nutritional and health-promoting quality of carrot (Daucus carotaL.) storage roots. The experiment (conducted in 2012-2014) comprised the soil fertilization of carrot ‘Kazan’ F1in the following combinations: 1. Control, 2. KI, 3. KIO3, 4. Na2SeO4, 5. Na2SeO3, 6. KI + Na2SeO4, 7. KIO3+ Na2SeO4, 8. KI + Na2SeO3, 9. KIO3+ Na2SeO3. Iodine and selenium were applied twice: before sowing and as top dressing in a total dose of 5 kg I ha−1and 1 kg Se ha−1. No significant influence of iodine and selenium fertilization was noted with respect to average root weight and leaf yield. Each year, the application of KI + Na2SeO4negatively affected the content of glucose and total sugars in carrot. An increased sucrose level was noted in the roots of plants treated with KIO3+ Na2SeO4, with a total sugar concentration comparable to the control. Irrespective of the year, carrots fertilized with KI were characterized by the highest accumulation of nitrates (III) – NO2−in roots. The simultaneous introduction of iodine and selenium compounds (KI + Na2SeO4, KIO3+ Na2SeO4, KI + Na2SeO3and KIO3+ Na2SeO3) into the soil reduced the content of nitrates (III) in carrot as compared to combinations with the individual application of these compounds. The influence of the tested factors on other analysed parameters (the content of dry weight, nitrates (V), chlorides, oxalates, citrates, free amino acids, carotenoids, phenolic compounds, phenylpropanoids, flavonols and anthocyanins as well as free radical scavenging activity (DPPH) was rather year-dependent.

Antioxidative and Antiviral Properties of Flowering Cherry Fruits (Prunus serrulataL. var.spontanea)

2010 ◽  
Vol 38 (05) ◽  
pp. 937-948 ◽  
Author(s):  
Hong-Sun Yook ◽  
Kyoung-Hee Kim ◽  
Jung-Eun Park ◽  
Hyun-Jin Shin
Keyword(s):  
Radical Scavenging Activity ◽  
Antiviral Agents ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Minimum Concentration ◽  
Diarrhea Virus ◽  
Prunus Yedoensis ◽  
Antiviral Activities ◽  
Flowering Cherry

The phenolic compounds of many fruits have been known to be efficient cellular protective antioxidants. In this study, antioxidative and antiviral properties of flowering cherry cultivars (Prunus yedoensis, Prunus sargentii, Prunus lannesiana, and Prunus cerasus) in Korea were investigated. The antioxidant property was assayed for specific activities including 2,2-diphenyl-1-picrylhydrazyl (DPPH) hydroxy radical scavenging activity, reducing power capacity, and superoxide dismutase (SOD) like activity. In addition, antiviral activity was determined by inhibition studies on the infection cycle of porcine epidemic diarrhea virus (PEDV), measured as minimum concentration of cherry extracts that inhibited 50% of cytopathic effect (CPE) on PEDV. Our results show that the four varieties of cherries contain substantially high antioxidants and antiviral activities. In particular, P. cerasus contains higher antioxidants and antiviral activities as well as polyphenolic content than other varieties. Our data indicate that Korean native cherry cultivars could be beneficial supplements of dietary antioxidants and natural antiviral agents.

scholarly journals In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia

2021 ◽  
pp. 281-291
Author(s):  
Rajendran Raja Priya ◽  
N. Bhadusha ◽  
Veramuthu Manivannan ◽  
Thanthoni Gunasekaran
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Hydroalcoholic Extract ◽  
Reducing Power Assay

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

scholarly journals Antioxidant Capacities of Jujube Fruit Seeds and Peel Pulp

2020 ◽  
Vol 10 (17) ◽  
pp. 6007 ◽  
Author(s):  
Yung-Sheng Lin ◽  
Wen-Shin Lin ◽  
Jing-Wen Tung ◽  
Ya-Chih Cheng ◽  
Min-Yun Chang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Jujube Fruit

In this study, the effects of different fruit parts and extraction conditions on the antioxidant properties of jujube (Ziziphus jujuba Mill.) fruit were investigated. Five in vitro antioxidant models and statistical analyses were performed. The results revealed that jujube peel with pulp (peel pulp) exhibited better antioxidant capacity than did seeds. Overall, jujube peel pulp extracted using 50% ethanol at 60 °C exhibited the best antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (0.3 ± 0 mg/mL), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity (0.5 ± 0 mg/mL), total phenolic content (38.3 ± 0.4 mg gallic acid equivalent per gram dry weight), total flavonoid content (43.8 ± 0.2 mg quercetin equivalent per gram dry weight), and reducing power (41.9 ± 2.2 mg ascorbic acid equivalent per gram dry weight). The results indicated that jujube peel pulp is a more potential natural antioxidant than seeds.

Antioxidant Activity and Chemical Composition of Helichrysum lacteum Coss. & Dur.

2020 ◽  
Vol 10 (4) ◽  
pp. 429-439
Author(s):  
Souad Arrif ◽  
Mohammed Benkhaled ◽  
Hamada Haba ◽  
Soumia Mouffouk
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Pure Compound ◽  
Etoac Extract ◽  
Total Antioxidant ◽  
Dpph Scavenging

Objective: : The objective of the present study was to measure antioxidant activity and to evaluate the phytochemical constitution of Helichrysum lacteum growing in Algeria. Methods: : The structure elucidation of the isolated compounds was performed by spectroscopic methods. Antioxidant activity of the extracts the crude extracts (PE, EtOAc, and n-BuOH) and pure compound (noryangonin (3)) was investigated by 5 methods, including DPPH free radical scavenging activity, reducing power and ferric thiocyanate methods, hydrogen peroxide scavenging capacity, and total antioxidant activity by phosphomolybdate assay. Results: : The chemical investigation of the EtOAc extract of the aerial parts of H. lacteum led to the isolation of two flavonoids, astragalin (1) and isoquercitrin (2), together with a styryl pyrone, noryangonin (3), and a cyclobutane dimer, achyrodimer C (4) as well as two triterpenoid compounds, β-sitosterol (5) and oleanolic acid (6). The highest phenolic and flavonoid contents were detected in the ethyl acetate extract. Noryangonin exhibited the highest antioxidant activity than those of the references and all the tested extracts, with a value of IC50 at 1.45±0.44 μg/mL in DPPH scavenging. In hydrogen peroxide scavenging essay, the results revealed that EtOAc extract exhibited the highest percent inhibition (63.15 %) as compared to the ascorbic acid (63.36 %) at the concentration of 100 μg/mL. In the phosphomolybdenum method, the EtOAc extract showed the strongest activity (36.85±0.02 μg EAA/mg ex) as compared to those of n-BuOH and PE extracts. Conclusion: : The antioxidant property shown in these findings needs further investigation, especially with the pure compound (3).

scholarly journals RAPID IN VITRO CALLOGENESIS AND PHYTOCHEMICAL SCREENING OF LEAF, STEM AND LEAF CALLUS OF MUSSAENDA FRONDOSA LINN. - A MEDICINAL PLANT.

2017 ◽  
Vol 10 (6) ◽  
pp. 81 ◽  
Author(s):  
Manasa Dj ◽  
Chandrashekar Kr ◽  
Bhagya N
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leaf Explants ◽  
Reducing Power ◽  
Callus Cultures ◽  
Phytochemical Analysis ◽  
Qualitative And Quantitative

Objective: To standardise the protocol for rapid callogenesis in Mussaenda frondosa L. using leaf explants. Qualitative and quantitative phytochemical analysis of leaf, stem and callus cultures.Methods: The leaf explants were inoculated onto MS medium supplemented with varying concentrations of growth regulators such as 2, 4 - D, NAA, BAP, Kn for the induction of callus. Qualitative and quantitative analysis of total phenol, flavonoids and alkaloids contents of leaf, stem and callus were tested by standard methods.  The antioxidant activities were investigated using DPPH radical scavenging method and reducing power assay. The anti - inflammatory activity was evaluated by membrane stabilizing activity.Results: Pale green, healthy, friable and fast growing callus was obtained on the medium enriched with NAA (2mg/l) + Kn (4mg/l). Quantitative determination showed the highest concentration of total phenolics in the methanolic extract of in vitro grown callus (10 ± 1.1 mg of GA/g of extract), flavonoids in methanolic stem extract (137±1.6 mg of Quercitin/g of extract) and alkaloids in methanolic extract of leaf (118.3±1.5 mg/10g of extract). The methanolic leaf extract exhibited highest free radical scavenging activity with IC50 value of 40.6±10.06 μg/ml. The highest membrane stabilizing activity was shown by chloroform extract of the leaf (66.02%).Conclusion: The present preliminary phytochemical and pharmacological analysis may form the basis for drug development in future using callus cultures of M. frondosa.   

scholarly journals Characterization of Phycocyanin Pigment from Marine Filamentous Cyanobacteria Geitlerinema sp.

2020 ◽  
Vol 2 (1) ◽  
pp. 74
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Natural Environments ◽  
Blue Green Algae ◽  
Marine Algal ◽  
Reducing Power Assay ◽  
Free Radical Scavenging Assay

Algae is a simple and vast group of photosynthetic organisms. Algae grow in most of the natural environments, including water, rocks, and soil, but interestingly also grow on and in other organisms. Their main habitats are freshwater, brackish and marine ecosystems, Also volcanic waters and salt waters. Among them, marine algae are recognized as a rich source of beneficial bioactive compounds. Marine algal originated compounds have been reported to exhibit various biological activities such as anticoagulant, anti-viral, antioxidant, anti-allergic, anti-cancer, anti-inflammatory, anti-obesity, etc. Phycocyanin is a blue-colored naturally existing phycobiliprotein pigment, which is abundant in cyanobacteria, blue-green algae. In this study, phycocyanin was extracted from the Geitlerinema sp. UV-Vis spectroscopic analysis and HPLC analysis confirmed the presence of phycocyanin. The extracted pigment was assessed for their antioxidant property by various methods such as Phosphomolybdenum assay, Ferric ions reducing power assay, DPPH radical scavenging activity, Hydrogen peroxide free radical scavenging assay, Anti-lipid peroxidation activity. The study reveals that the phycocyanin pigments possess the antioxidant property, and it can be used as a promising pharmaceutical and nutraceutical compound.

scholarly journals PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF CLERODENDRUM PANICULATUM (L.) LEAF AND FLOWER EXTRACTS

2021 ◽  
pp. 31-34
Author(s):  
DEEPA R HEBBAR ◽  
DEVIKA M ◽  
RASHMI GN ◽  
NALINI MS
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Hexane Extracts ◽  
Reducing Power Assay

Objective: This study was designed to evaluate the phytochemicals present in the flower and leaf extracts of Clerodendrum paniculatum L., collected from Nelji village of Kodagu district . Methods: The healthy leaves and flowers of C. paniculatum were collected and the plant extracts were prepared using ethanol, hexane and distilled water separately. Phytochemical analysis was conducted using standard procedurs for the flower and leaf extracts of C. paniculatum. The antioxidant activity in leaf and flower extracts was determined by three assays, estimation of total phenolic content, reducing power assay and radical scavenging activity (DPPH) using standard procedures. Results: Phytochemical screening conducted for the flower and leaf extracts of C. paniculatum showed the presence of three phytochemicals, namely saponins, alkaloids and terpenoids. Terpenoids were commonly present in all the extracts of flower and leaf that is in both polar (aqueous and ethanol) solvent and in non-polar (hexane) solvents. The extracts tested for the antioxidant activity showed the presence of total phenolics in ethanol, aqueous and hexane extracts. The aqueous extract showed high redox potential followed by ethanol and hexane extracts. The aqueous leaf extract showed high radical scavenging activity when compared to the flower extracts of C. paniculatum. Conclusion:  The present study showes C. paniculatum to be an important medicinal plant, since the flower and leaves showed good antioxidant activity. Thus it may used in the treatment of diseases and may also used in the preparation of natural or herbal drugs due to the presence of antioxidants.

Fortification of a Desert Using Nanoencapsulated Supercritical Carbon Dioxide Extract of Small Cardamom Seeds: A Nutraceutical Custard with Antioxidant Synergy

2021 ◽  
Vol 15 ◽  
Author(s):  
Kaninika Paul ◽  
Dipshikha Tamili ◽  
Paramita Bhattacharjee
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Control Sample ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Antioxidant Potency ◽  
Spray Dried

Background: 1,8 cineole-rich supercritical CO2 extract of small cardamom seeds of Alleppey green variety exhibiting prominent antioxidant property was microencapsulated and utilized in formulating an antioxidant-rich custard. However, the antioxidant potency of the prepared custard was not appreciable. To redress the phytochemical loss during custard preparation, custard using nanoliposomes was formulated. Patents related to 1,8 cineole-rich food products have been revised thoroughly. Objective: The objective of the current study is to examine whether nanoencapsulation-mediated entrapment of antioxidants is more effective in fortifying a dessert, namely custard, vis-à-vis microencapsulated (spray dried)-mediated enhancement of antioxidative potency in the same. Methods: Our previous investigations have established that nanoliposome of 1,8 cineole-rich supercritical CO2 extract of small cardamom seeds effectively redresses type 2 diabetes and hypercholesterolemia. In the current investigation, this pre-characterized nanoliposome which exhibited appreciable in vitro and in vivo antioxidant efficacy has been utilized at varying concentrations for fortification of a custard. The designer custard samples have been characterized for their sensory and physicochemical properties, identification of the cardamom antioxidants therein and determination of the synergistic efficacy value of the identified antioxidants. Results: The custard formulated with 0.3% nanoliposomes exhibited appreciable antioxidant potency in terms of DPPH radical scavenging activity (304.58±1.09 mg/ml) and reducing power (0.020±0.001 mg BHT/g custard), conferred by its total phenolic content (0.049±0.004 mg GAE/g custard). It also had relatively more stable textural attributes vis-à-vis the control sample (formulated with the non-encapsulated native extract). GC-MS analysis of the nanoliposome-fortified custard confirmed retention of the spice antioxidants namely1,8- cineole, α-terpinyl acetate, α-terpineol and linalool and its synergistic efficacy value being greater than unity, attested to a synergistic presence of cardamom antioxidants therein. The newly formulated custard retained more than 4.5 times of 1,8-cineole (5.05 mg/g custard) vis-à-vis the custard sample (1.12 mg/g custard) prepared with a microencapsulated (spray-dried) formulation of the extract. Additionally, absence of heavy metals in the formulated custard confirmed it to be safe for consumption. Conclusion: This is the first study on application of nanoliposomes of spiceuticals in formulation of a dessert, and more emphatically on use of a ‘green’ supercritical CO2 extract of spice antioxidants in fortification of a dessert to achieve antioxidant synergy.

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