Performance of Strobilanthes crispus Leaves Extract as Potential Antioxidant Compound in Oil-in-water Emulsion

Excess of free radicals accelerates the degradation of oil-in-water (O/W) emulsion due to oxidative damage resulting in low emulsion quality such as in lotion formulation. The blend of antioxidants in O/W emulsion could reduce the damage caused by the radical compounds. The crude extract of Strobilanthes crispus (S. crispus) leaves was screened for assessing bioactive phytochemical constituents. By using various chemical assays, the extract of S. crispus leaves revealed the presence of tannins, steroids, flavonoids, terpenoids and glycosides. Besides that, the antioxidant activity of the leaf extract, spray-dried extract, a lotion containing extract and other local and abroad commercial lotions were determined as references using standard methods such as total phenolic content (TPC), total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and reducing power assay. In terms of TFC, the highest, 10.591±0.01 mg QE/g was recorded in crude extract along with the highest reducing power and percentage inhibition, 25.40±0.02 mg GAE/g and 88.89 % respectively. The result from TPC value can be a good indicator for a longer shelf life of the O/W emulsion if compared to other results from TPF, reducing power and DPPH value. From this study, it can be concluded that S. crispus has potential as a good natural antioxidant ingredient for the emulsion of O/W formulation.

In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF CLERODENDRUM PANICULATUM (L.) LEAF AND FLOWER EXTRACTS

Objective: This study was designed to evaluate the phytochemicals present in the flower and leaf extracts of Clerodendrum paniculatum L., collected from Nelji village of Kodagu district . Methods: The healthy leaves and flowers of C. paniculatum were collected and the plant extracts were prepared using ethanol, hexane and distilled water separately. Phytochemical analysis was conducted using standard procedurs for the flower and leaf extracts of C. paniculatum. The antioxidant activity in leaf and flower extracts was determined by three assays, estimation of total phenolic content, reducing power assay and radical scavenging activity (DPPH) using standard procedures. Results: Phytochemical screening conducted for the flower and leaf extracts of C. paniculatum showed the presence of three phytochemicals, namely saponins, alkaloids and terpenoids. Terpenoids were commonly present in all the extracts of flower and leaf that is in both polar (aqueous and ethanol) solvent and in non-polar (hexane) solvents. The extracts tested for the antioxidant activity showed the presence of total phenolics in ethanol, aqueous and hexane extracts. The aqueous extract showed high redox potential followed by ethanol and hexane extracts. The aqueous leaf extract showed high radical scavenging activity when compared to the flower extracts of C. paniculatum. Conclusion: The present study showes C. paniculatum to be an important medicinal plant, since the flower and leaves showed good antioxidant activity. Thus it may used in the treatment of diseases and may also used in the preparation of natural or herbal drugs due to the presence of antioxidants.

Comparison of phenolic content and antioxidant activity of two common fruits of Bangladesh in solvents of varying polarities

Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus emblica and Elaeocarpus floribundus, were measured in water, methanol, ethanol, acetone and hexane extracts. Several in vitro models including phosphomolybdenum assay, DPPH free radical scavenging assay, FRAP assay and reducing power assay were used to assess the antioxidant activity of these extracts in comparison with reference antioxidants. Between the two fruits, P. emblica showed higher phenolic content and antioxidant activity in all the solvents used. In the DPPH scavenging assay, the activity of P. emblica extracts was close to reference antioxidants, ascorbic acid and BHT. Besides, considering the solvents used, extracts of both fruits had the highest phenolic and antioxidant activity in polar solvents. The correlation coefficient between total phenolics and antioxidant activities was found statistically significant. These findings suggest that P. emblica could be an excellent antioxidant resource for industries like food, pharmaceutical, and cosmetics.

Phytochemicals, Antioxidant and Antidiabetic Activities of Extracts from Miliusa velutina Flowers

The flowers of M. velutina were extracted with ethanol to obtain a crude extract that was consecutively extracted using n-hexane, dichloromethane, ethyl acetate and water. The crude extract and fractions were studied for the chemical composition and antioxidant and antidiabetic activities. The extracts had various phytoconstituents, namely steroids, flavonoids, tannins, saponins, alkaloids and glycosides. The aqueous extract had the highest total polyphenol (12.6 mg GAE/g extract) and total flavonoid (205.6 mg QE/g extract) content. The aqueous extract exhibited the strongest antioxidant activities in the ferric reducing antioxidant power assay (EC50 = 4.0 µg/mL), reducing power assay (EC50 = 78.1 µg/mL), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid radical cation assay (EC50 = 48.2 µg/mL), total antioxidant capacity assay (EC50 = 8.7 µg/mL) and 1,1-diphenyl-2-picrylhydrazyl assay (EC50 = 9.3 µg/mL). The aqueous extract showed the strongest inhibitory effect on the activity of α-amylase (IC50 = 376.6 μg/mL) and α-glucosidase (IC50 = 69.7 μg/mL). The results showed that the aqueous extract of M. velutina flowers can be a promising candidate for the control of diabetes and oxidative stress. This is the first report about the chemical components and antioxidant and antidiabetic activities of M. velutina flower extracts.

Phytochemical content and antioxidant activities of Rhubarb (Rheum emodi)

Medicinal plants have varying natural products and several antioxidants. Antioxidants play a principal function to protect against destruction caused by oxidative stress (OS), <i>Rheum emodi</i> is not an exception, in which it is reported to have compounds that possess antioxidant activity, like polyphenolic compounds. In addition to that, other compounds have proven to have antidiabetic, antimicrobial, antifungal, cytotoxic, hepatoprotective, and nephroprotective activities. This study aimed to quantify the water extract of a powdered plant of <i>R. emodi</i> to evaluate its ability to scavage free radicals. Total phenolic, total flavonoid contents, and reducing ability were measured to consider possible sources of future novel antioxidants in plants. The TPC, TFC, and reducing power assay increased with increasing concentrations of <i>R. emodi</i>. At 1000 µg/ml, the absorbance ranged from 1.437 for TPC, 1.602 for TFC, and 1.638 for reducing power assay, which is more than the absorbance of the standards at the same concentration. The extracted content of reducing power, phenolic, and flavonoid compounds were higher than the standard pyrogallol, quercetin, and ascorbic acid.

Assessment of Anti-oxidant Activity of Aerial Parts of Mangrove Plant, Derris trifoliata ( Leguminosae)

The current study was conducted to verify the traditional medicinal use and to carry out the in-vitro antioxidant activity of various solvent extracts of Derris trifoliata (aerial part). The percentage yield of ethanol, ethyl acetate and n-hexane extracts were found 2.5% w/w. Freshly prepared extracts were subjected to preliminary phytochemical screening. All extracts revealed the presence of several important phytochemicals which might be responsible for its medicinal properties. In vitro Electron transfer (ET) reaction-based assays of ethanol, ethyl acetate and n-hexane extracts have been investigated using various model systems viz., DPPH, total phenolic, tannin and flavonoid content, ferric ion reducing antioxidant power (FRAP) and reducing power assay. Hydrogen atom transfer (HAT) reaction-based assays have been conducted using Nitric Oxide (NO) scavenging and hydrogen peroxide scavenging activity assay methods. Ethanol extract was found to possess highest DPPH (IC50=16.824 µg/ml), total phenolic content (44.51 GAE/g of dried plant extract), reducing power assay (0.387±0.0006), FRAF assay (IC50=133.51 µg/ml), hydrogen peroxide scavenging (IC50=144.888 µg/ml) and nitric oxide scavenging activities (IC50=152.655 µg/ml). Whereas ethyl acetate extract was found to possess the highest total tannin content (42.56 GAE/g of dried plant extract) and total flavonoid content (78.08 QE/g of dried plant extract). In vitro antioxidant study was also performed in terms of chelation power on ferrous ions. The highest chelation power was found for ethyl acetate extract (IC50=62.489 µg/ml). The above study suggests that Derris trifoliata may be a vital source of nutraceuticals.

In vitro anti-inflammatory, anti-diabetic and antioxidant potential of Cissus quadrangularis along with its orexigenic activity in Drosophila melanogaster

Plants with diverse pharmacological activities are actively being explored for human health. Cissus quadrangularis (L) has been reported to possess numerous phytochemicals and is used to relieve various disorders. This article aims at providing evidence of the diverse pharmacological activities in terms of orexigenic, anti-inflammatory, anti-diabetic and antioxidant activities of C. quadrangularis for further application in clinical development. The results revealed that inhibition of hemolysis was within the range of 8-9-25.6% at concentrations of 12.5-200 µg/ml. Methanol extract of C. quadrangularis stems exhibited porcine pancreatic ?-amylase (PPA) inhibition (p?0.05) at concentrations of 0.25 and 0.30 mg/ml. The glucose adsorption capacity of the C. quadrangularis was observed to be inversely proportional to the molar concentration of glucose. The higher food intake by Drosophila in food medium with plant extract is presumably related to orexigenic property of C. quadrangularis. Protease activity of C. quadrangularis stem extract revealed total activity 975 U/ml and specific activity as 3768 U/mg. The absorbance of C. quadrangularis in reducing power assay were between 0.91and 1.85. Highest total antioxidant activity of 67.2 µg TE/g was observed and the hydroxyl radicals scavenging activity was observed in a dose dependent manner. The results provide supporting data that C. quadrangularis may contain active compounds useful in treating anti-inflammatory, anti-diabetic disorders.

Phytochemical Study, HPLC Chromatographic Analysis and Antioxidant Activity of Ephedra alata DC. Female Cones Extracts

The aim of this work is a phytochemical study, antioxidant activity and Chromatographic Analysis by HPLC of extracts of the female cones of Ephedra alata DC. Which grows in Oued Souf region (South East of Algeria). According to the results obtained from the estimation of polyphenols and flavonoids content, the methanolic extract has the best content of polyphenols (158.34±2.71mg GAE/g Extract), and the best values of flavonoids (88.50±1.12mg QE/g Extract). The chromatographic analysis by HPLC has identified eight phenolic compounds, which are Gallic Acid, Chlorogenic acid, Vanillic Acid, Vanillin, p-Coumaric acid, Rutin, Naringenin and Quercitin. The antioxidant activity was evaluated by three tests: DPPH• free-radical scavenging, Hemolysis and Reducing power, the results of test scavenging the free-radical DPPH• show the tannins extract had the best scavenging activity capacity than the other extracts (IC50: 14.94±1.34μg/mL), But, in the hemolysis test, all the extracts were proximity except for the aqueous extract that was shown protected of the erythrocytes (50±0.5% of hemolysis percentage). Finally, in the reducing power assay, its results showed that the tannins extract has a best reducing power 27.16±0.25μg/mL in Abs700= 0.5 compared to other extracts.