Short-Term Intervention with Garlic-Based diets Modulates mRNA Expression of Calmodulin, Inositol-1,4,5-Trisphosphate Receptor-1, and Intracellular Adhesion Molecule in Cyclosporin-Induced Prehypertensive Wistar Rats

2021 ◽  
Vol 05 (04) ◽  
Author(s):  
Ajayi O.B. ◽  
Ajayi O.O
Keyword(s):  
Mrna Expression ◽  
Adhesion Molecule ◽  
Wistar Rats ◽  
Short Term ◽  
Intracellular Adhesion Molecule ◽  
Inositol 1,4,5 Trisphosphate ◽  
Trisphosphate Receptor

Inositol 1,4,5-trisphosphate receptor and ryanodine receptor in the aging brain of Wistar rats

1994 ◽  
Vol 15 (2) ◽  
pp. 203-206 ◽  
Author(s):  
Adelina Martini ◽  
Fiorenzo Battaini ◽  
Stefano Govoni ◽  
Pompeo Volpe
Keyword(s):  
Ryanodine Receptor ◽  
Wistar Rats ◽  
Aging Brain ◽  
Inositol 1,4,5 Trisphosphate ◽  
Trisphosphate Receptor

scholarly journals Characterization of inositol 1,4,5-trisphosphate receptor isoform mRNA expression and regulation in rat pancreatic islets, RINm5F cells and betaHC9 cells

1998 ◽  
Vol 21 (1) ◽  
pp. 31-39 ◽  
Author(s):  
B Lee ◽  
PG Bradford ◽  
SG Laychock
Keyword(s):  
Insulin Secretion ◽  
Beta Cell ◽  
Mrna Expression ◽  
Dependent Manner ◽  
Cell Type ◽  
Rinm5f Cells ◽  
Concentration Dependent Manner ◽  
Type Iii ◽  
Inositol 1,4,5 Trisphosphate ◽  
Trisphosphate Receptor

The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+ channel that plays a role in the regulation of insulin secretion. In rat isolated pancreatic islets the expression of types I, II and III InsP3R mRNA was identified by reverse transcriptase-polymerase chain reaction and confirmed by cDNA cloning and sequencing. The islet ratios of types I, II and III InsP3R mRNA to beta-actin mRNA were 0.08 +/- 0.02, 0.08 +/- 0.03 and 0.25 +/- 0.04 respectively. Types I, II and III InsP3R mRNA were also expressed in rat (RINm5F) and mouse (betaHC9) pancreatic beta-cell lines, and rat cerebellum. Type III InsP3R mRNA was quantitatively the most abundant form in rat islets and RINm5F cells. In betaHC9 cells, types II and III InsP3R mRNA were expressed at similar levels, and in much greater abundance than type I mRNA. Type III was the least abundant InsP3R mRNA in cerebellum. Culture of betaHC9 cells for 5 days at 2.8 and 25 mM glucose, or RINm5F cells for 7 days at 5.5 and 20 mM glucose, resulted in significantly enhanced expression of type III, but not types I and II, InsP3R mRNA in the cells at the higher glucose concentrations. During short-term (0.5-2 h) incubations, betaHC9 cell type III InsP3R mRNA levels increased in response to glucose in a time- and concentration-dependent manner. Actinomycin D inhibited the glucose response. Alpha-ketoisocaproic acid also stimulated betaHC9 cell type III InsP3R mRNA expression in a concentration-dependent manner, whereas 2-deoxyglucose and 3-O-methylglucose were without effect. The different levels of expression of mRNA for three InsP3R isoforms in islets and insulinoma cells, and the influence of glucose and alpha-ketoisocaproic acid on the expression of type III mRNA, suggests that nutrient metabolism plays a role in the regulation of this gene and that the function of InsP3R subtypes may be unique with each playing a distinct role in beta-cell signal transduction and insulin secretion.

scholarly journals Effects from the induction of heat shock proteins in a murine model due to progression of aortic atherosclerosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Naoya Hashikawa ◽  
Masanobu Ido ◽  
Yuna Morita ◽  
Narumi Hashikawa-Hobara
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Mrna Expression ◽  
Adhesion Molecule ◽  
Intercellular Adhesion Molecule ◽  
Aortic Atherosclerosis ◽  
Short Term ◽  
Shock Proteins ◽  
The Relationship ◽  
Progression Of Atherosclerosis

AbstractHeat shock proteins (HSPs) are molecular chaperones that repair denatured proteins. The relationship between HSPs and various diseases has been extensively studied. However, the relationship between HSPs and atherosclerosis remains unclear. In this study, we induced the expression of HSPs and analyzed the effects on the development/progression of atherosclerosis in vivo. Remarkably, when HSPs were induced in apolipoprotein E deficient (ApoE−/−) mice prior to the formation of atheromas, the progression of atherosclerosis was inhibited; the short-term induction of HSPs significantly decreased the mRNA expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in the aorta. In contrast, the induction of HSPs after the formation of atheromas promoted the progression of atherosclerosis. In fact, the short-term induction of HSPs, after the formation of atheromas, significantly increased the mRNA expression of tumor necrosis factor-alpha, and interleukin 6 in the aorta. Of note, the induction of HSPs also promoted the formation of macrophage-derived foam cells. Overall, these results indicate that HSPs exerts different effects in the context of aortic atherosclerosis, depending on its degree of progression. Therefore, the induction and inhibition of HSPs should be considered for the prevention and treatment of atherosclerosis, respectively.

Polychlorinated biphenyl induced hepatocyte alterations

1987 ◽  
Vol 45 ◽  
pp. 716-717
Author(s):  
D.N. Collins ◽  
J.N. Turner ◽  
K.O. Brosch ◽  
R.F. Seegal
Keyword(s):  
Lipid Droplets ◽  
Wistar Rats ◽  
Homovanillic Acid ◽  
Polychlorinated Biphenyl ◽  
Corn Oil ◽  
Environmental Pollutants ◽  
Short Term ◽  
Pcb Congeners ◽  
Urinary Levels ◽  
The Brain

Polychlorinated biphenyls (PCBs) are a ubiquitous class of environmental pollutants with toxic and hepatocellular effects, including accumulation of fat, proliferated smooth endoplasmic recticulum (SER), and concentric membrane arrays (CMAs) (1-3). The CMAs appear to be a membrane storage and degeneration organelle composed of a large number of concentric membrane layers usually surrounding one or more lipid droplets often with internalized membrane fragments (3). The present study documents liver alteration after a short term single dose exposure to PCBs with high chlorine content, and correlates them with reported animal weights and central nervous system (CNS) measures. In the brain PCB congeners were concentrated in particular regions (4) while catecholamine concentrations were decreased (4-6). Urinary levels of homovanillic acid a dopamine metabolite were evaluated (7).Wistar rats were gavaged with corn oil (6 controls), or with a 1:1 mixture of Aroclor 1254 and 1260 in corn oil at 500 or 1000 mg total PCB/kg (6 at each level).

Sign in / Sign up

Export Citation Format

Share Document