Isolation and speciation of candida from various clinical samples by using conventional method and chrom agar in a tertiary care centre

2021 ◽  
Vol 20 (1) ◽  
pp. 06-10
Author(s):  
Gunapravathy Istalingam I ◽  
Keyword(s):  
Conventional Method ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Centre ◽  
Tertiary Care Centre

Mupirocin resistance in clinical isolates of methicillin sensitive and resistant Staphylococcus aureus in a tertiary care centre of North India

JMS SKIMS ◽  
2019 ◽  
Vol 22 (3) ◽  
Author(s):  
Nargis Bali ◽  
Maroof Peer ◽  
Roseleen Kour ◽  
Suhail Ahmad ◽  
Parvaiz Koul
Keyword(s):  
Staphylococcus Aureus ◽  
Tertiary Care ◽  
Clinical Isolates ◽  
North India ◽  
Clinical Samples ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Low Level ◽  
Mupirocin Resistance ◽  
Level Resistance

Background: Staphylococcus aureus continues to be major healthcare problem worldwide as therapeutic options for its management are limited. Decolonisation regimes using mupiroicin are aimed at reducing the burden of infection due to this microorganism. However resistance to mupirocin poses a threat to the current standard eradication protocols.Aims: To find out the type and extent of mupirocin resistance in clinical isolates of S. aureus. Material and methods: This cross-sectional study was carried out at a tertiary care centre of North India for a period of 6 months. S. aureus recovered by standard microbiological procedures form various clinical samples such as blood, urine, pus and other body fluids was subjected to antimicrobial susceptibility testing along with detection of mupirocin resistance using 5 and 200µg discs. Minimum inhibitory concentration (MIC) of mupirocin was determined using E -test strips. Fisher’s exact test was done to determine the statistical significance. A P-value of <0.05 was considered as statistically significant.Results: A total of 247 S. aureus isolates were recovered of which 81 (32.8%) were resistant to methicillin (MRSA) whereas 166 (67.2%) were sensitive to this antibiotic (MSSA). Low level resistance to mupirocin was seen in 7 (8.6%) MRSA and 3 (1.8%) MSSA isolates. Prior use of this topical antibiotic and previous hospitalisation were found to be the risk factors associated with mupirocin resistance. Conclusion: Low level mupirocin resistance was seen in S. aureus isolates which if left unchecked could in future pave way for high level resistance and subsequent treatment failures.

scholarly journals Meropenem Resistance among Acinetobacter Positive Clinical Samples in a Tertiary Care Centre in Nepal: A Descriptive Cross-sectional Study

2021 ◽  
Vol 59 (241) ◽  
pp. 853-857
Author(s):  
Nisha Sharma ◽  
Bibechan Thapa ◽  
Ashirbad Acharya ◽  
Bijendra Raj Raghubanshi
Keyword(s):  
Confidence Interval ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Clinical Samples ◽  
Care Centre ◽  
Sectional Study ◽  
Tertiary Care Centre ◽  
Cross Sectional ◽  
Acinetobacter Species ◽  
Tract Infections

Introduction: Antimicrobial-resistant Acinetobacter species are implicated in a variety of infections including nosocomial bacteraemia, secondary meningitis, and urinary tract infections. Carbapenem including meropenem-resistant Acinetobacter is recognized as one of the most difficult antimicrobial resistant gram-negative bacilli to control and treat. It was classified as an urgent threat by Centers for Disease Control and Prevention in 2019 Antibiotic Resistance Threats Report. This study was carriedout to determine the prevalence of meropenem resistance among acinetobacter positive clinical samples in a tertiary care centre. Methods: A descriptive cross-sectional study was carried out in microbiology department of Clinical Laboratory Services among Acinetobacter positive clinical samples of a tertiary care center in Nepal. The culture and sensitivity reports of various clinical samples from April 2018 to April 2020 which were positive for Acinetobacter species were taken from hospital records section. Convenience sampling was done. Meropenem-resistant Acinetobacter samples were studied. Ethical approval was received from Institutional Review Committee (Ref No. 076/77/40). Analysis of data was done using Statistical Package for the Social Sciences version 26. Point estimate at 95% Confidence Interval calculated with ferquency. Results: Out of 121 Acinetobacter isolates, prevalence of meropenem-resistant Acinetobacter was reported in 93 (76.9%) at 95% Confidence Interval (69.39-84.40). Among the meropenem-resistant Acinetobacter samples, most of the samples were collected from the sputum 70 (75.2%) followed by blood 8 (8.6%). Conclusions: High prevalence of meropenem-resistant Acinetobacter species in our hospital setting is alarming. In addition, there is emergence of resistance against even the last resort drugs which is creating a treatment crisis.

Abstract #1100: Tumor Induced Osteomalacia: Clinical; Biochemical and Radiological Features from a Single Tertiary Care Centre with Emphasis on Functional Imaging.

2017 ◽  
Vol 23 ◽  
pp. 289
Author(s):  
Vineet Surana ◽  
Rajesh Khadgawat ◽  
Nikhil Tandon ◽  
Chandrashekhar Bal ◽  
Kandasamy Devasenathipathy
Keyword(s):  
Functional Imaging ◽  
Tertiary Care ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Radiological Features
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