In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines

Background: Silver nanoparticles play a significant role in bioavailability and refining the compatibility of natural drugs in the treatment of various chronic diseases including different types of cancer. Objective: Green synthesis of silver nanocomposites of Nigella sativa seeds extract to evaluate the anticancer effects against hepatocellular carcinoma using HepG2 cell lines. Methods: The AgNCs were developed by treating aqueous extract of N. sativa seeds treated with silver nitrate (1mM) solution and were used to test its efficacy against hepatocellular carcinoma using HepG2 cell lines. Results and Discussion: The Surface Plasmon Resonance (SPR) of prepared AgNCs showed a peak at 432 nm via UV spectroscopy. The selected N. sativa AgNCs were characterized for polydispersity, surface charge and size and the results showed 0.215±0.093 polydispersity index (PDI), zeta potential 18.8±0.372 mV and size range 10-20 nm, respectively. The Fourier transform infrared spectroscopy (FTIR) also showed various peak of functional groups that are possibly involved in the reduction of silver ion and synthesized the N. sativa silver nanocomposites, respectively. N. sativa AgNCs showed 89.954% drug release while in the case of extract release, it was only 33.821% in 24 hrs. Further, in vitro studies of N. sativa AgNCs against hepatocellular carcinoma showed good cytotoxic effect p<0.05 with 7.16 µg/ml IC50 value. Conclusion: Thus, the present results revealed that green synthesis of N. sativa AgNCs can be an alternative tool for clinical application in cancer therapy; however, there is a need to find the mechanism and role of AgNCs inside the individual.

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IN VITRO CYTOTOXICITY ASSAY, ANTIOXIDANT SCREENING AND HEPATOPROTECTIVE ACTIVITY OF SUCCESSIVE EXTRACTS OF ALYSICARPUS VAGINALIS VAR. NUMMULARIFOLIUS (DC.) MIQ. (FAMILY: FABACEAE) IN HEPG2 CELL LINES

International Journal of Biology Pharmacy and Allied Sciences ◽

10.31032/ijbpas/2021/10.1.5298 ◽

2021 ◽

Vol 10 (1) ◽

Keyword(s):

Hepg2 Cell ◽

Cell Lines ◽

Hepatoprotective Activity ◽

In Vitro Cytotoxicity ◽

Cytotoxicity Assay ◽

Hepg2 Cell Lines

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In vitro cellular models of human hepatic fatty acid metabolism: differences between Huh7 and HepG2 cell lines in human and fetal bovine culturing serum

Physiological Reports ◽

10.14814/phy2.13532 ◽

2017 ◽

Vol 5 (24) ◽

pp. e13532 ◽

Cited By ~ 17

Author(s):

Pippa J. Gunn ◽

Charlotte J. Green ◽

Camilla Pramfalk ◽

Leanne Hodson

Keyword(s):

Fatty Acid ◽

Hepg2 Cell ◽

Cell Lines ◽

Fatty Acid Metabolism ◽

Acid Metabolism ◽

Cellular Models ◽

Hepatic Fatty Acid ◽

Fetal Bovine ◽

Hepg2 Cell Lines

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One-Pot Synthesis of 5-(Het)Aryl 8-Aminoquinoline Amide Derivatives as Potential Antibacterial / Cytotoxic Agents

Current Bioactive Compounds ◽

10.2174/1573407214666180910130225 ◽

2020 ◽

Vol 16 (2) ◽

pp. 142-151

Author(s):

Zanjam Spandana ◽

Tadigiri M. Rekha ◽

Mandava V.B. Rao ◽

Manojit Pal

Keyword(s):

Hepg2 Cell ◽

Cell Lines ◽

Antibacterial Activities ◽

Cytotoxic Agents ◽

Hek293 Cells ◽

Cytotoxic Activities ◽

Quinoline Derivatives ◽

One Pot ◽

Hepg2 Cell Lines

Background: The 8-Aminoquinoline (8-AQ) framework has attracted particular attention in the discovery and development of antimalarial and anti-bacterial agents or drugs. However, the clinical uses of 8-AQ based drugs are often associated with toxic side effects such as methemoglobinemia and hemolytic anemia with deficiency in Glucose-6-Phosphate Dehydrogenase (G6PD) Activity. The 4-aryl- 8-amino(acetamido)quinoline derivatives, on the other hand, have shown antiproliferative activities against cancer cell lines. These reports prompted us to assess the antibacterial and cytotoxic activities of a series of compounds based on 5-aryl 8-aminoquinoline amide scaffold. Methods: A series of compounds based on 5-(het)aryl 8-aminoquinoline amide scaffold was synthesized via a one-pot ultrasound-assisted method using a C-5 selective halogenation of quinoline derivatives followed by Pd/C-catalyzed Suzuki-Miyaura coupling with (het)aryl boronic acids. All these compounds were evaluated for their in vitro antibacterial activities against representative Gram-(+) and Gram-(-) strains including Escherichia coli, Pseudomonas aeruginosa, Klebsiella species and Staphylococcus aureus. Three compounds were further tested for cytotoxicities in vitro against breast adenocarcinoma (MCF7) and Hepatocellular Carcinoma (HepG2) along with non-cancerous human embryonic kidney (HEK293) cell lines. Results: All these compounds demonstrated moderate to good antibacterial activities against the four organisms used. In vitro assay results revealed that three compounds showed good activities against Gram-(+) strains and Gram-(-) strains and one was comparable to ciprofloxacin and pefloxacin. These three compounds were further tested for their cytotoxic properties against MCF7 and HepG2 cell lines. One of them showed IC50 value comparable to doxorubicin when tested against HepG2 cell lines. However, none of these compounds showed any significant effects when tested against HEK293 cells indicating their selectivity towards the growth inhibition of cancer cells. Conclusion: A series of compounds based on 5-(het)aryl 8-aminoquinoline amide scaffold was synthesized and evaluated for antibacterial and cytotoxic activities. Several of these compounds showed promising antibacterial and cytotoxic activities when tested in vitro suggesting that the present class of compounds may be of interest for the identification of new and potential antibacterial / cytotoxic agents.

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