IN VITRO CYTOTOXICITY ASSAY, ANTIOXIDANT SCREENING AND HEPATOPROTECTIVE ACTIVITY OF SUCCESSIVE EXTRACTS OF ALYSICARPUS VAGINALIS VAR. NUMMULARIFOLIUS (DC.) MIQ. (FAMILY: FABACEAE) IN HEPG2 CELL LINES

Background: Silver nanoparticles play a significant role in bioavailability and refining the compatibility of natural drugs in the treatment of various chronic diseases including different types of cancer. Objective: Green synthesis of silver nanocomposites of Nigella sativa seeds extract to evaluate the anticancer effects against hepatocellular carcinoma using HepG2 cell lines. Methods: The AgNCs were developed by treating aqueous extract of N. sativa seeds treated with silver nitrate (1mM) solution and were used to test its efficacy against hepatocellular carcinoma using HepG2 cell lines. Results and Discussion: The Surface Plasmon Resonance (SPR) of prepared AgNCs showed a peak at 432 nm via UV spectroscopy. The selected N. sativa AgNCs were characterized for polydispersity, surface charge and size and the results showed 0.215±0.093 polydispersity index (PDI), zeta potential 18.8±0.372 mV and size range 10-20 nm, respectively. The Fourier transform infrared spectroscopy (FTIR) also showed various peak of functional groups that are possibly involved in the reduction of silver ion and synthesized the N. sativa silver nanocomposites, respectively. N. sativa AgNCs showed 89.954% drug release while in the case of extract release, it was only 33.821% in 24 hrs. Further, in vitro studies of N. sativa AgNCs against hepatocellular carcinoma showed good cytotoxic effect p<0.05 with 7.16 µg/ml IC50 value. Conclusion: Thus, the present results revealed that green synthesis of N. sativa AgNCs can be an alternative tool for clinical application in cancer therapy; however, there is a need to find the mechanism and role of AgNCs inside the individual.

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In vitro cellular models of human hepatic fatty acid metabolism: differences between Huh7 and HepG2 cell lines in human and fetal bovine culturing serum

Physiological Reports ◽

10.14814/phy2.13532 ◽

2017 ◽

Vol 5 (24) ◽

pp. e13532 ◽

Cited By ~ 17

Author(s):

Pippa J. Gunn ◽

Charlotte J. Green ◽

Camilla Pramfalk ◽

Leanne Hodson

Keyword(s):

Fatty Acid ◽

Hepg2 Cell ◽

Cell Lines ◽

Fatty Acid Metabolism ◽

Acid Metabolism ◽

Cellular Models ◽

Hepatic Fatty Acid ◽

Fetal Bovine ◽

Hepg2 Cell Lines

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One-Pot Synthesis of 5-(Het)Aryl 8-Aminoquinoline Amide Derivatives as Potential Antibacterial / Cytotoxic Agents

Current Bioactive Compounds ◽

10.2174/1573407214666180910130225 ◽

2020 ◽

Vol 16 (2) ◽

pp. 142-151

Author(s):

Zanjam Spandana ◽

Tadigiri M. Rekha ◽

Mandava V.B. Rao ◽

Manojit Pal

Keyword(s):

Hepg2 Cell ◽

Cell Lines ◽

Antibacterial Activities ◽

Cytotoxic Agents ◽

Hek293 Cells ◽

Cytotoxic Activities ◽

Quinoline Derivatives ◽

One Pot ◽

Hepg2 Cell Lines

Background: The 8-Aminoquinoline (8-AQ) framework has attracted particular attention in the discovery and development of antimalarial and anti-bacterial agents or drugs. However, the clinical uses of 8-AQ based drugs are often associated with toxic side effects such as methemoglobinemia and hemolytic anemia with deficiency in Glucose-6-Phosphate Dehydrogenase (G6PD) Activity. The 4-aryl- 8-amino(acetamido)quinoline derivatives, on the other hand, have shown antiproliferative activities against cancer cell lines. These reports prompted us to assess the antibacterial and cytotoxic activities of a series of compounds based on 5-aryl 8-aminoquinoline amide scaffold. Methods: A series of compounds based on 5-(het)aryl 8-aminoquinoline amide scaffold was synthesized via a one-pot ultrasound-assisted method using a C-5 selective halogenation of quinoline derivatives followed by Pd/C-catalyzed Suzuki-Miyaura coupling with (het)aryl boronic acids. All these compounds were evaluated for their in vitro antibacterial activities against representative Gram-(+) and Gram-(-) strains including Escherichia coli, Pseudomonas aeruginosa, Klebsiella species and Staphylococcus aureus. Three compounds were further tested for cytotoxicities in vitro against breast adenocarcinoma (MCF7) and Hepatocellular Carcinoma (HepG2) along with non-cancerous human embryonic kidney (HEK293) cell lines. Results: All these compounds demonstrated moderate to good antibacterial activities against the four organisms used. In vitro assay results revealed that three compounds showed good activities against Gram-(+) strains and Gram-(-) strains and one was comparable to ciprofloxacin and pefloxacin. These three compounds were further tested for their cytotoxic properties against MCF7 and HepG2 cell lines. One of them showed IC50 value comparable to doxorubicin when tested against HepG2 cell lines. However, none of these compounds showed any significant effects when tested against HEK293 cells indicating their selectivity towards the growth inhibition of cancer cells. Conclusion: A series of compounds based on 5-(het)aryl 8-aminoquinoline amide scaffold was synthesized and evaluated for antibacterial and cytotoxic activities. Several of these compounds showed promising antibacterial and cytotoxic activities when tested in vitro suggesting that the present class of compounds may be of interest for the identification of new and potential antibacterial / cytotoxic agents.

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Effects of cancer-testis antigen, TFDP3, on cell cycle regulation and its mechanism in L-02 and HepG2 cell lines in vitro

PLoS ONE ◽

10.1371/journal.pone.0182781 ◽

2017 ◽

Vol 12 (8) ◽

pp. e0182781 ◽

Cited By ~ 2

Author(s):

Yunshen Jiao ◽

Lingyu Ding ◽

Ming Chu ◽

Tieshan Wang ◽

Jiarui Kang ◽

...

Keyword(s):

Cell Cycle ◽

Hepg2 Cell ◽

Cell Lines ◽

Cell Cycle Regulation ◽

Cancer Testis Antigen ◽

Hepg2 Cell Lines ◽

Cycle Regulation ◽

Cancer Testis

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Synthesis and in vitro cytotoxicity evaluation of isatin-pyrrole derivatives against HepG2 cell line

Open Chemistry ◽

10.1515/chem-2021-0023 ◽

2021 ◽

Vol 19 (1) ◽

pp. 199-204

Author(s):

Mardi Santoso ◽

Arif Fadlan ◽

Muhammad Riza Ghulam Fahmi ◽

Ardhana Rahmayanti

Keyword(s):

Liver Cancer ◽

Hepg2 Cell ◽

Human Liver ◽

In Vitro Cytotoxicity ◽

Moderate Activity ◽

Hepg2 Cell Line ◽

Pyrrole Derivatives ◽

Human Liver Cancer ◽

Hepg2 Cell Lines

Abstract This paper reports the synthesis and in vitro cytotoxicity evaluation of isatin-pyrrole derivatives 5–8, obtained from the appropriate isatins with pyrrole, with good yields and purity. The product structures were confirmed through spectroscopy methods. Furthermore, the MTT assay on the human liver cancer HepG2 cell lines revealed moderate activity in all compounds, which was highest in sample 6 (IC50 0.47 µM). The anticancer activity was affiliated with the presence of a nitro group at C-5 and N-methyl of the isatin scaffold.

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IN VITRO HEPATOPROTECTIVE EFFECT OF ECHINOCHLOA COLONA ON ETHANOL-INDUCED OXIDATIVE DAMAGE IN HEPG2 CELLS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i9.19322 ◽

2017 ◽

Vol 10 (9) ◽

pp. 259 ◽

Cited By ~ 1

Author(s):

Praneetha Pallerla ◽

Durgaih G ◽

Narsimha Reddy Y ◽

Ravi Kumar B

Keyword(s):

Hepg2 Cell ◽

Hepatoprotective Activity ◽

Standard Drug ◽

Cytoprotective Activity ◽

Cytotoxicity Studies ◽

Maximum Protection ◽

Diphenyl Tetrazolium Bromide ◽

Hepg2 Cell Lines ◽

Dose Dependent

Objective: The current study was aimed to evaluate the methanolic extract of caryopses of Echinochloa colona (ECME) for its in vitro hepatoprotective activity against ethanol in HepG2 cell lines.Methods: In this regard, the cytotoxicity studies were conducted for the extract, ECME using 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide assay to determine the inhibitory concentration 50% value based on which, the doses 50, 100, and 200 μg/ml were selected for the hepatoprotective studies in HepG2 cell lines. The toxicity was induced using ethanol (100 mM). The in vitro hepatoprotective activity of the extract was assessed based on the changes in the level of biochemical parameters such as aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase.Results: The extract, ECME has shown a dose-dependent cytoprotective activity with maximum protection at 200 μg/ml. The percentage cell viability of the extract, ECME at 200 μg/ml was more, i.e., 69.33% which was well comparable to that of standard drug, silymarin (100 μg/ml).Conclusion: The study revealed that the extract had shown significant hepatoprotective activity at all the test doses against ethanol-induced cytotoxity assay.

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SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay

Molecules ◽

10.3390/molecules25030653 ◽

2020 ◽

Vol 25 (3) ◽

pp. 653 ◽

Cited By ~ 1

Author(s):

Ana-Maria Putz ◽

Cătălin Ianăși ◽

Zoltán Dudás ◽

Dorina Coricovac ◽

Claudia (Farcas) Watz ◽

...

Keyword(s):

Cell Viability ◽

Cell Line ◽

Cell Lines ◽

Drug Carriers ◽

In Vitro Cytotoxicity ◽

Mri Contrast Agents ◽

Cytotoxicity Assay ◽

Tumour Cell Line ◽

Vitro Assay

A facile sol-gel route has been applied to synthesize hybrid silica-PVA-iron oxide nanocomposite materials. A step-by-step calcination (processing temperatures up to 400 °C) was applied in order to oxidize the organics together with the iron precursor. Transmission electron microscopy, X-ray diffraction, small angle neutron scattering, and nitrogen porosimetry were used to determine the temperature-induced morpho-textural modifications. In vitro cytotoxicity assay was conducted by monitoring the cell viability by the means of MTT assay to qualify the materials as MRI contrast agents or as drug carriers. Two cell lines were considered: the HaCaT (human keratinocyte cell line) and the A375 tumour cell line of human melanoma. Five concentrations of 10 µg/mL, 30 µg/mL, 50 µg/mL, 100 µg/mL, and 200 µg/mL were tested, while using DMSO (dimethylsulfoxid) and PBS (phosphate saline buffer) as solvents. The HaCaT and A375 cell lines were exposed to the prepared agent suspensions for 24 h. In the case of DMSO (dimethyl sulfoxide) suspensions, the effect on human keratinocytes migration and proliferation were also evaluated. The results indicate that only the concentrations of 100 μg/mL and 200 μg/mL of the nanocomposite in DMSO induced a slight decrease in the HaCaT cell viability. The PBS based in vitro assay showed that the nanocomposite did not present toxicity on the HaCaT cells, even at high doses (200 μg/mL agent).

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