Caries protective effect of two Self-etch dentin bonding agents on denuded root dentin surface: In Vitro Study

2019 ◽  
Vol 11 (2) ◽  
pp. 36-41
Author(s):  
Dr. Jini P M ◽  
Dr. Shibani Shetty ◽  
Dr. Jayalakshmi K B ◽  
Dr. Prasannalatha Nadig ◽  
Dr. Sujatha I
Keyword(s):  
Protective Effect ◽  
In Vitro Study ◽  
Bonding Agent ◽  
Control Group ◽  
Bonding Agents ◽  
Dental Adhesive ◽  
Dentin Bonding ◽  
Group A ◽  
Root Dentin

Objective: To evaluate the caries protective effect of two self-etching dental adhesive agents on the denuded root surface. Materials and methods: The roots of 30 freshly extracted , non-carious human premolars were cleaned and denuded of cementum. The teeth surfaces were covered with acid-resistant nail varnish, exposing two rectangular windows (lingual and buccal surface) of 4 x 4 mm each. The window on the lingual furface served as the control , while the buccal window were treated with dentin bonding agent. All specimens were store in acidifed gel for 6 days for demineralization. The specimens were then divided among two experimental groups-Group A: Optibond all in one and Group B: Bond-1SF.In Group C (control group) bonding agent was not applied. The specimens were stored in 0.9% sodium chloride solution for 14 days. Plano parallel tooth sections (80 ± 20 μm) were obtained. Tooth sections were evaluated for caries‑like demineralized zone under a polarized microscope. The values obtained were analyzed using one‑way ANOVA and Tukey honesty tests. Results: In the Control group the mean depth of demineralization was 80.08µm .The depth of demineralization was significantly reduced in Group A and B. Conclusion: Self-etching dental adhesive agents with and without solvent is an effective method to prevent demineralization of root dentin.

scholarly journals Evaluation of caries – protective effect of three recent dentin bonding agents, xeno v plus, tetric n bond ,one coat seven universal on demineralized root surface "- An in vitro study

2019 ◽  
pp. 29-41
Author(s):  
Tanvi Dugge ◽  
Balaram Naik
Keyword(s):  
Protective Effect ◽  
Root Surface ◽  
Bonding Agent ◽  
Control Group ◽  
Human Teeth ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Rectangular Window ◽  
Root Surfaces

Background and objectives: An area on the surface of tooth, apical to CEJ, where root caries are bound to occur undergoes clinically apparent dissolution of the calcified tissues. When dentin bonding agent is applied, it forms finger like resin tags protruding into patent dentin tubules When this resin penetrates the superficially demineralized collagen matrix, it forms a hybrid layerand prevents root surface lesions. Aim: To evaluate the caries protective effect of three recent DBAs on demineralized root surface. Materials and methods: 88 single rooted extracted human teeth were selected, root surfaces apical to CEJ were polished .Acid resistant nail paint wascoated over root surfaces exposing a rectangular window on the buccal surface. Three experimental groups made: Group 1 (XENO V PLUS), Group 2 (TETRIC N BOND), Group 3 (ONE COAT 7 UNIVERSAL). These groups were divided into 2 Subgroups (A and B). Adhesive was applied and light cured with air thinning. Over this second layer of adhesive was applied, light cured with /without air thinning. Each tooth samples were sliced. A light microscope was used to examine the caries like lesions.The statistical analysis was carried out. Results:All control group specimen showed lesions with a mean depth of 177.74μm. Subgroup B results were better than A and C. Summary and Conclusion: Demineralization on root surface can be impeded by DBA tested TetricN Bond which showed maximum cariesprotective effect Key words:Cementum, demineralization, dentine bonding agent

scholarly journals Hydroxyl and calcium ions diffusion from endodontic materials through roots of primary teeth - in vitro study

2005 ◽  
Vol 13 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Ana Cristina Gerent Petry Nunes ◽  
Maria José de Carvalho Rocha
Keyword(s):  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Apical Surface ◽  
Working Length ◽  
Group A ◽  
Periapical Healing ◽  
Materials Used ◽  
Spectrometer Data

The purpose of this research was to evaluate the diffusion of calcium (Ca+2) and hydroxyl (OH-) ions from materials with a calcium hydroxide base - Ca(OH)2 through the intact roots of deciduous teeth. This diffusion of ions is important for periapical healing. Forty-six deciduous teeth were selected and instrumented to their working length with #40 files. The teeth were washed during cleaning and shaping with a 1% sodium hypochlorite (NaOCl) solution. The canals were dried with paper points. The teeth were divided into 4 groups based on the sealer type, with 10 specimens in each group. A fifth group of 6 teeth without sealer constituted the control group. The materials used as sealers were: Ca(OH)2 paste associated to propylene glycol (CaPE) thickened at the proportion of 2:1 w/v; UFSC (Federal University of Santa Catarina) paste - a mixture of 0.3g of zinc oxide with 0.3g of Ca(OH)2 with 0.2ml of olive oil 1:1 w/w; Vitapex® and Sealapex®. The coronal access was sealed with a glass ionomer after the root had been filled with each sealer. A one-third apical surface and foramen was hard-pressed with Araldite®. The teeth were stored individually in flasks containing saline solution at 37ºC and 100% humidity. The OH- and Ca+2 ions diffusion levels were determined using a pH meter and an atomic absorption spectrometer. Data were collected at 48 hours and at intervals of 7, 30, 45 and 60 days. Statistical analysis was performed using ANOVA to compare groups. In the pH evaluation, the CaPE group presented the largest OH- ions diffusion, which peaked at sixty days (p=0.0309), when compared to the other groups (p<0.0001). In relation to amount of Ca+2 ions released, the CaPE paste showed the best results, followed by the UFSC's paste. These results suggest that the CaPE paste was the material that allowed the highest diffusion of OH- and Ca+2 ions.

scholarly journals Comparative Evaluation of Fluoride Release From Chemically Cured and Light-Cured Orthodontic Bonding Agents and Surface Alteration of Enamel: An in vitro Study

2020 ◽  
Vol 54 (3) ◽  
pp. 233-239
Author(s):  
Ashish Kumar Barik ◽  
Ritu Duggal
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Fluoride Content ◽  
Bonding Agent ◽  
Fluoride Release ◽  
Bonding Agents ◽  
Surface Alteration ◽  
Orthodontic Bonding ◽  
Group B

Introduction: Fluoride is known to reduce demineralization and enhance remineralization which is desirable around orthodontic brackets. Material and methods: This study was carried out to determine the rate of fluoride release, fluoride content of enamel, and surface alteration of enamel from orthodontic bonding agents. Thirty extracted maxillary first premolars were divided into 2 groups of 15 teeth each. Group A—teeth were bonded with a chemically cured orthodontic bonding agent containing fluoride (Rely-a-Bond, Reliance, Itasca) and group B—teeth were bonded with a light-cured orthodontic bonding agent containing fluoride (Light Bond Reliance, Itasca). All individual sample teeth were used to estimate fluoride release and fluoride content of enamel. The same samples were also used to evaluate enamel surface alterations. The rate of fluoride release from the above 2 orthodontic bonding agents were measured in artificial saliva on day 1, 2, 3, 4, 5, 6, 7, 14, 30, 60, and 90. The fluoride content of the enamel was evaluated on day “0” and day “90” of bonding. The samples were also evaluated for the surface changes in enamel using scanning electron microscope (SEM). Results: The results showed that (a) chemically cured and light-cured bonding agent showed a dramatic decrease in the concentration of fluoride release from day “1” to day “2,” (b) the concentration of fluoride release kept decreasing from day “1” to day “90” of measurement in both the groups, and (c) SEM of the bracket margins bonded with chemically cured bonding agent and light-cured bonding agent revealed particle depositions of irregular shape and size on day “90.” Conclusion: Light-cured bonding agent (Light Bond) released higher concentration of fluoride as compared to chemically cured bonding agent (Rely-a-Bond). Light-cured bonding agent (Light Bond) showed higher increase in fluoride content of enamel than chemically cured bonding agent (Rely-a-Bond).

scholarly journals Radiopacity evaluation of composite restorative resins and bonding agents using digital and film x-ray systems

2012 ◽  
Vol 06 (02) ◽  
pp. 115-122 ◽  
Author(s):  
Bengi Oztas ◽  
Sebnem Kursun ◽  
Gul Dinc ◽  
Kıvanc Kamburoglu
Keyword(s):  
Composite Materials ◽  
In Vitro Study ◽  
Composite Resins ◽  
Human Tooth ◽  
Dental Resin ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Dentin Bonding Agents ◽  
Phosphor Plates

ABSTRACTObjective: The purpose of this in vitro study was to explore the radiopacity of composite resins and bonding materials using film and phosphor plates.Methods: Nine composite dental resin specimens and human tooth slices were exposed together with an aluminium stepwedge using dental film and phosphor plates. Eight dentin bonding specimens were prepared and exposed in a similar manner. Their radiopacity on film was assessed using a transmission densitometer, and the radiopacity with phosphor plates was assessed digitally using the system’s own software (Digora). Data were analysed using one-way analysis of variance (ANOVA) and post-hoc Tukey tests (P<.05). Film and phosphor plate radiopacity values were compared using simple regression analysisResults: Excellent linear correlation was found between film and phosphor plates for both composite resins and bonding agents. The composite materials Spectrum Tph and Natural Look exhibited the highest radiopacity with film and with phosphor plates, respectively. All the dentin bonding agents tested exhibited lower radiopacity than dentin. Conclusion: Synergy, Ice, Filtek Silorane, Filtek Z250, Clearfil Majesty Posterior, Herculite Classic, Spectrum Tph, and Natural Look composite materials exhibited greater radiopacity than dentin, and all the dentin bonding agents tested exhibited lower radiopacity than either enamel or dentin. (Eur J Dent 2012;6:115-122)

EVALUATION OF THE EFFECT OF FERROUS SULFATE ON ENAMEL DEMINERALIZATION OF HUMAN DECIDUOUS TEETH: AN IN VITRO STUDY

2017 ◽  
Vol 8 (3) ◽  
pp. 83-89
Author(s):  
Johnny Holanda De Gauw ◽  
Lara Maria Melo Costa ◽  
Rodrigo Neves Silva ◽  
Natanael Barbosa Santos ◽  
Maria Dânia Holanda Tenorio
Keyword(s):  
Ferrous Sulfate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Group A ◽  
Ph Cycling ◽  
Group B ◽  
Group D

Objective: This study aimed to evaluate the effect of ferrous sulfate (FS) on demineralized and non-demineralized human deciduous teeth. Additionally, it was evaluated the penetration extent of FS and its remineralizing effect on the enamel of deciduous teeth using Polarized Light Microscopy (PLM). Method: The sample comprised 44 human deciduous teeth. The 44 crowns were divided randomly into four groups: group A (FS after demineralization), group B (FS without demineralization), group C (only demineralization), and group D (control group). FS at 0.45 mol/L-1 was used daily (15 days) and demineralization was done by pH cycling (7 days). Then, three longitudinal slices of the crowns were photographed using PLM. The degree of penetration of the lesion or stain was measured in micrometers, as well as the distance between the external enamel surface and the core of lesion. Results: Group A showed a dark stain on the outer surface of enamel larger than the group B. It is suggested, a remineralizing effect when comparing groups, A and C. The mean depth and standard deviation for groups A, B, and C were 4.27µm (±1.49), 3.72 µm (±1.68) and 5.00 µm (±1.84), respectively. No dark stains were observed in group D. Conclusion: FS stained the demineralized and non-demineralized human deciduous teeth. However, dark stains in the non-demineralized teeth were smaller or absent, than in the demineralized teeth. Therefore, FS may have a protective effect against demineralization.

scholarly journals Effect of 5% Calcium Hypochlorite on Mechanical Properties of Root Dentin: An in vitro Study

2016 ◽  
Vol 1 (2) ◽  
pp. 56-59
Author(s):  
Natanasabapathy Velmurugan ◽  
Poornima Reddy ◽  
Suma Balla ◽  
Sandhya S Raghu ◽  
Garlapati T Gupta ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Flexural Strength ◽  
Modulus Of Elasticity ◽  
In Vitro Study ◽  
Chlorine Concentration ◽  
Control Group ◽  
Calcium Hypochlorite ◽  
Significant Difference ◽  
Root Dentin

ABSTRACT Introduction The aim of this study was to compare in vitro the effect of 5% calcium hypochlorite [Ca(OCl)2] and 5% sodium hypochlorite (NaOCl) on flexural strength and modulus of elasticity of root dentin. Materials and methods The available chlorine concentration of each solution was determined using iodometric titration. Standardized planoparallel dentin bars (n= 20) were divided into two test groups and one control group. The control, group 1, consisted of dentin bars stored in normal saline. The dentin bars in the two test groups were treated by exposure to following solutions: Group 2 to 5% Ca(OCl)2; and group 3 to 5% NaOCl. All the three test solutions were changed once in 15 minutes for 30 minutes. The dentin bars were then loaded to failure using three-point bend test. Results Available chlorine concentration was 64% in both the test solutions. There was a significant reduction in the flexural strength of 5% NaOCl group compared to 5% Ca(OCl)2-treated ones. A significant difference in modulus of elasticity was observed between the test groups and the control groups and also between the 5% Ca(OCl)2 and 5% NaOCl groups. Conclusion Within the limitations of this study, 5% NaOCl reduced the flexural strength and modulus of elasticity of root dentin bars more when compared to 5% Ca(OCl)2. How to cite this article Reddy P, Balla S, Raghu SS, Velmurugan N, Gupta GT, Sahoo HS. Effect of 5% Calcium Hypochlorite on Mechanical Properties of Root Dentin: An in vitro Study. J Oper Dent Endod 2016;1(2):56-59.

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