EVALUATION OF THE EFFECT OF FERROUS SULFATE ON ENAMEL DEMINERALIZATION OF HUMAN DECIDUOUS TEETH: AN IN VITRO STUDY

2017 ◽  
Vol 8 (3) ◽  
pp. 83-89
Author(s):  
Johnny Holanda De Gauw ◽  
Lara Maria Melo Costa ◽  
Rodrigo Neves Silva ◽  
Natanael Barbosa Santos ◽  
Maria Dânia Holanda Tenorio
Keyword(s):  
Ferrous Sulfate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Group A ◽  
Ph Cycling ◽  
Group B ◽  
Group D

Objective: This study aimed to evaluate the effect of ferrous sulfate (FS) on demineralized and non-demineralized human deciduous teeth. Additionally, it was evaluated the penetration extent of FS and its remineralizing effect on the enamel of deciduous teeth using Polarized Light Microscopy (PLM). Method: The sample comprised 44 human deciduous teeth. The 44 crowns were divided randomly into four groups: group A (FS after demineralization), group B (FS without demineralization), group C (only demineralization), and group D (control group). FS at 0.45 mol/L-1 was used daily (15 days) and demineralization was done by pH cycling (7 days). Then, three longitudinal slices of the crowns were photographed using PLM. The degree of penetration of the lesion or stain was measured in micrometers, as well as the distance between the external enamel surface and the core of lesion. Results: Group A showed a dark stain on the outer surface of enamel larger than the group B. It is suggested, a remineralizing effect when comparing groups, A and C. The mean depth and standard deviation for groups A, B, and C were 4.27µm (±1.49), 3.72 µm (±1.68) and 5.00 µm (±1.84), respectively. No dark stains were observed in group D. Conclusion: FS stained the demineralized and non-demineralized human deciduous teeth. However, dark stains in the non-demineralized teeth were smaller or absent, than in the demineralized teeth. Therefore, FS may have a protective effect against demineralization.

scholarly journals Erosive potential of three different commonly used pediatric syrups on deciduous teeth enamel: an in vitro study

2020 ◽  
Vol 7 (10) ◽  
pp. 2041
Author(s):  
Neha Thilak ◽  
Sundeep K. Hedge ◽  
Sham S. Bhat
Keyword(s):  
In Vitro Study ◽  
Titratable Acidity ◽  
Deciduous Teeth ◽  
Hardness Tester ◽  
Titrable Acidity ◽  
Group A ◽  
Group B ◽  
Post Hoc ◽  
Group D

Background: The aim of the study is to compare the erosive potential of three different commonly used pediatric syrups on deciduous teeth enamel. The objectives of the study were to assess the endogeneous pH and titratable acidity of mefenamic acid syrup (meftal P), cetrizine syrup (alerid) and multivitamin syrup (zincovit) and to evaluate the microhardness of the enamel after successive immersion cycles in each of the syrups.Methods: 40 non carious deciduous teeth were included for the study The samples were then randomly allocated into 4 groups (10 in each group): Group A- mefenemic acid syrup (meftal P), Group B- cetrizine syrup (alerid), group C- multivitamin syrup (zincovit) and group D- control (distilled water). The samples were then subjected to the immersion cycles in the syrups. Assessment of enamel surface microhardness was done using Vickers hardness tester at 7th day and 14th day. The pH and titrable acidity of the syrups were also assessed. One way analysis of variance (ANOVA) and post hoc tests were used for the statistical analysis.Results: Out of the test groups, group C showed the lowest pH of around 4.2 and exhibited the largest titrable acidity (22.8 ml) compared with 21 ml in group A and 15.5 ml in group B. At the end of 14th day, group A had microhardness of about 293.43.84±6.34, group B had 299.930±6.85, group C had 313.380±6.23 and group D had 334.190±5.51.Conclusions: All the pediatric liquid medications assessed in the study, meftal P, alerid and zincovit showed acidic pH, high titrable acidities and all the syrups showed loss of microhardness after exposure to the syrups for 14 days. Loss of microhardness was highest for meftal P followed by alerid and least for zincovit.

scholarly journals Zeolite as a Bone Bio-Modifier Carrier: An In-Vitro Study

2019 ◽  
Vol 9 (1) ◽  
pp. 126
Author(s):  
Mobina Mousavi ◽  
Azadeh Esmaeil Nejad ◽  
Erfan Shamsoddin ◽  
Mohammad Mehdi Golabgiran ◽  
Behzad Houshmand
Keyword(s):  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Alizarin Red ◽  
Group A ◽  
Group B ◽  
And Function ◽  
Group D

Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear. Methods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1μg/mL Z+A, Group B: 0.1μg/mL Z without A, Group C: 0.2μg/mL Z+A, Group D: 0.2μg/mL Z without A, Group E: 0.3μg/mL Z+A, Group F: 0.3μg/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P<0.05). Results: At 24 hours, results showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p<0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p<0.001). Conclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.

Comparison of CPP-ACP, Tri-Calcium Phosphate and Hydroxyapatite on Remineralization of Artificial Caries Like Lesions on Primary Enamel -An in vitro Study

2016 ◽  
Vol 40 (5) ◽  
pp. 404-409 ◽  
Author(s):  
Meghna Bajaj ◽  
P Poornima ◽  
S Praveen ◽  
NB Nagaveni ◽  
KB Roopa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Before And After ◽  
Group B ◽  
Group D

Objectives: To compare CPP-ACP, Tri-calcium phosphate and Hydroxyapatite on remineralization of artificial caries like lesions on primary enamel. Study design: Ten extracted Primary molars coated with nail varnish, leaving a window of 2×4 mm on buccal and lingual surface were immersed in demineralizing solution for 96 hours and sectioned longitudinally to obtain 40 sections (4 sections per tooth) and were randomly divided into 4 groups (A to D) n=10; Group A: negative control, Group B: CPP-ACP, Group C: Tri-calcium phosphate, Group D: Hydroxyapatite. Sections were subjected to pH cycling for 10 days and were evaluated by polarized light microscope before and after treatment. Results: Intra group comparison of demineralization and remineralization was done by paired t-test. One way ANOVA was used for multiple group comparisons followed by post HOC TUKEY'S Test for group wise comparisons. Remineralization was found more with Group D followed by Group B, C and A. Conclusion: Hydroxyapatite showed better remineralization when compared to CPP-ACP and Tri-calcium phosphate.

scholarly journals Hydroxyl and calcium ions diffusion from endodontic materials through roots of primary teeth - in vitro study

2005 ◽  
Vol 13 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Ana Cristina Gerent Petry Nunes ◽  
Maria José de Carvalho Rocha
Keyword(s):  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Apical Surface ◽  
Working Length ◽  
Group A ◽  
Periapical Healing ◽  
Materials Used ◽  
Spectrometer Data

The purpose of this research was to evaluate the diffusion of calcium (Ca+2) and hydroxyl (OH-) ions from materials with a calcium hydroxide base - Ca(OH)2 through the intact roots of deciduous teeth. This diffusion of ions is important for periapical healing. Forty-six deciduous teeth were selected and instrumented to their working length with #40 files. The teeth were washed during cleaning and shaping with a 1% sodium hypochlorite (NaOCl) solution. The canals were dried with paper points. The teeth were divided into 4 groups based on the sealer type, with 10 specimens in each group. A fifth group of 6 teeth without sealer constituted the control group. The materials used as sealers were: Ca(OH)2 paste associated to propylene glycol (CaPE) thickened at the proportion of 2:1 w/v; UFSC (Federal University of Santa Catarina) paste - a mixture of 0.3g of zinc oxide with 0.3g of Ca(OH)2 with 0.2ml of olive oil 1:1 w/w; Vitapex® and Sealapex®. The coronal access was sealed with a glass ionomer after the root had been filled with each sealer. A one-third apical surface and foramen was hard-pressed with Araldite®. The teeth were stored individually in flasks containing saline solution at 37ºC and 100% humidity. The OH- and Ca+2 ions diffusion levels were determined using a pH meter and an atomic absorption spectrometer. Data were collected at 48 hours and at intervals of 7, 30, 45 and 60 days. Statistical analysis was performed using ANOVA to compare groups. In the pH evaluation, the CaPE group presented the largest OH- ions diffusion, which peaked at sixty days (p=0.0309), when compared to the other groups (p<0.0001). In relation to amount of Ca+2 ions released, the CaPE paste showed the best results, followed by the UFSC's paste. These results suggest that the CaPE paste was the material that allowed the highest diffusion of OH- and Ca+2 ions.

The Effect of Varying Ferrule Modes on Fracture Resistance of Canines Restored with One-Piece Milled Zirconia Post and Core

2012 ◽  
Vol 624 ◽  
pp. 98-102
Author(s):  
Yao Kun Zhang ◽  
Long Quan Shao ◽  
Ruo Yu Liu ◽  
Lin Lin Wang ◽  
Jun Ai ◽  
...  
Keyword(s):  
Fracture Resistance ◽  
In Vitro Study ◽  
Significant Difference ◽  
Zirconia Crowns ◽  
Group A ◽  
Post And Core ◽  
Resistance Decrease ◽  
Group B ◽  
Group D

The aim of this invitro study was to evaluate the fracture resistance of endodontically treated canines restored with one-piece milled zirconia post and core in the varying ferrule modes. Fifty recently extracted human maxillary canines were endodontically treated and randomly divided into 5 groups of 10 specimens each. According to the defect degree of ferrule, intact ferrule was classified as group A and served as control, 2 mm ferrule height without buccal ferrule was classified as group B, 2 mm ferrule height without mesial and buccal ferrule was classified as group C, 2 mm ferrule height without mesial, buccal and lingual ferrule was classified as group D, no ferrule preparation was classified as group E. All of the teeth were restored with one-piece milled zirconia post and core, and zirconia crowns. The teeth were prepared to standardized specifications. The restored teeth were loaded to fracture at a 135° angle to their long axis, at a cross-head speed 0.5 mm/min, and the load (N) at failure was recorded. Statistical analysis was performed by SNK pairwise multiple comparisons (α=0.05). The mean fracture resistance of five groups as follows: 1019.30±139.01N for group A, 861.20±105.67N for group B, 833.70±100.56N for group C, 733.20±96.91N for group D, 698.00±99.90N for group E. The fracture resistance of restored teeth between group B and group C, group D and group E had no statistically significant difference(P>0.05). The fracture resistance of the rest pairwise group comparisons had statistically significant difference(P<0.05). Increasing the absence degree of ferrule, it was found that the fracture resistance decrease. The buccal and lingual ferrule were more important than mesial ferrule for enhanced fracture resistance. This in vitro study supports evidence that reserving the buccal and lingual ferrule is a key point to increase the fracture resistance of canines restored with one-piece milled zirconia post and core.

scholarly journals Effect of Honey in the Management of Otomycosis

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Amisha Patel ◽  
Vaghela D B
Keyword(s):  
Hearing Loss ◽  
Intervention Group ◽  
In Vitro Study ◽  
Signs And Symptoms ◽  
Control Group ◽  
Humid Climate ◽  
Group A ◽  
Clinical Control Trial ◽  
Group B

Otomycosis is a fungal infection of External ear, characterized by earache, pruritus, ear blockage, discharge, hearing loss and ringing in ear.  It is the most common clinical entity in the ENT clinics. The disease is more common in hot and humid climate. Its worldwide prevalence is 5.2 % and 9 % in India. 5-25 % of otitis externa cases are due to otomycosis. Bactericidal and fungicidal activity of Honey is proved previously by in vitro study. Honey has high osmolarity and acidic pH. And fungus does not grow in acidic media. With this background, present study is aimed to know the efficacy of the Honey in the management of Otomycosis. This randomized clinical control trial including two groups, one is intervention group (group A) and second is control group (group B). Pt is advised to instill three drops of Honey thrice a day for 7 days in Group A and three drops of Clotrimazole thrice a day for 7 days in Group B. There is statistically highly significant results was observed in all signs and symptoms except hearing loss which is significant in both Groups. Statistically insignificant difference was found between both Groups. 

scholarly journals Assessing Fracture Resistance of Restored Premolars with Novel Composite Materials: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Zahraa Abdulaali Al-Ibraheemi ◽  
Huda Abbas Abdullah ◽  
Nada Abdlameer Jawad ◽  
Julfikar Haider
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
In Vitro Study ◽  
Vitro Study ◽  
Resistance Testing ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Group D

During restorative treatment, premolars restored with resin filling materials using the conventional incremental-fill technique take longer restoration time and undermine the integrity of the tooth. The aim of this study was to assess fracture resistance of premolars restored by various types of novel bulk-fill composite resin materials. Forty-eight (n = 48) freshly extracted sound maxillary first premolars were used in this in vitro study. The teeth were divided into six groups, each having 8 specimens. Group A (positive control) was allocated for the intact teeth. For specimens in Groups B to F, a large cavity (Class-II MOD) was prepared with a standardized dimension of cavity (3 mm depth on the pulpal floor, 4 mm at the gingival seat, and 3 mm cavity width). Group B represented prepared teeth without any restoration. Group C, Group D, Group E, and Group F were restored with Tetric EvoCeram® incremental-fill (conventional), Beautifil bulk-fill, Filtek posterior bulk-fill, and SonicFill 2 bulk-fill restorative materials, respectively. All samples were finished and polished with an enhanced finishing kit and stored in distilled water for a month before the fracture resistance testing. All the samples were exposed to the axial loading (the speed of crosshead was 1 mm/min) in a computer-controlled universal testing machine (LARYEE, China) via a steel bar (6 mm in diameter) and the maximum applied force in Newton was recorded as the fracture resistance. One-way analysis of variance (SPSS 21) was used to compare the fracture resistance within the groups, and Tukey’s post hoc test was used to determine the difference between the groups. The lowest value of fracture resistance was recorded for Group B, and the highest value was recorded for Group A followed by the values of Group D, Group C, Group F, and Group E. One-way ANOVA revealed a statistically significant difference between the groups ( P < 0.05 ). Nonsignificant difference was found between the premolars restored by bulk-fill and conventional composites. Among the bulk-fill restored specimens, Beautifil restorative demonstrated significantly higher fracture resistance in comparison with the other two bulk-fill restored specimen groups (SonicFill 2 and Filtek). Bulk-fill composite such as Beautifil could be an alternative option to conventional incremental-fill composite for premolar restoration.

An in vitro study of microleakage of pit and fissure sealants in the presence of occlusal forces

2000 ◽  
Vol 24 (4) ◽  
pp. 273-278 ◽  
Author(s):  
Christina Zervou ◽  
Eileen Doherty ◽  
Athanasios Zavras ◽  
George White
Keyword(s):  
Cross Section ◽  
In Vitro Study ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants ◽  
Dye Penetration ◽  
Wisdom Teeth ◽  
Group A ◽  
Group B ◽  
Group D

The purpose of this study was to evaluate the effect of load on microleakage of pit and fissure sealants. In the first phase of the study load of maximal force (F = 880 N) on central and peripheral enamel was applied. Three groups of ten specimens were tested for microleakage. Group A no application of load, Group B application of load on the central pit of the tooth, and Group application of load on the peripheral enamel 2.5 mm away from the margin of the tooth, at the level of dentinoenamel junction. The results of that study showed significant increase of microleakage on the peripheral sealed enamel, as well as enamel microcracks. So, in the phase II sixty wisdom teeth were sealed with Ultra seal XT plus and load of different magnitude was applied on the peripheral enamel. The specimens were thermocycled for 500 cycles at 5°C and 55°C with a dwell of 30 seconds at each temperature prior to load application. Six groups of ten specimens were tested for microleakage under load. The force was applied at the peripheral enamel, 2.5 mm away from the margin of the tooth, at the level of dentin-enamel junction in cross-section view. Group A: F = 0 N; Group B: F = 300 N; Group C: F = 400 N; Group D: F = 500 N; Group E: F = 600 N; Group F: F = 700 N. Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underlying fissure. A linear regression statistics was used with R-squared of 0.31. The groups with load applied (B, C, D, E, F) showed statistically significant results (p&lt;0.05) compared with group A (F = 0 N). Both, the magnitude of the force and tooth morphology may influence the degree of microleakage observed after the placement of sealants.

scholarly journals Efficacy of Palatal Applications on Fracture Resistance of Reattached Maxillary Central Incisors: AnIn VitroStudy

2017 ◽  
Vol 2017 ◽  
pp. 1-5
Author(s):  
İhsan Yikilgan ◽  
Nagihan Guven ◽  
Cemile Kedıcı Alp ◽  
Emre Tokar ◽  
Ozgur Topuz ◽  
...  
Keyword(s):  
Fracture Strength ◽  
Fracture Resistance ◽  
Composite Resin ◽  
In Vitro Study ◽  
Plane Normal ◽  
Fixed Distance ◽  
Group A ◽  
Group B ◽  
Group D

The purpose of this study was to evaluate the effects of different palatal applications on fracture strength of the fractured anterior tooth. Sixty caries-free human maxillary incisors were used. Endodontic treatments of the teeth were performed. Then, the teeth were divided randomly into five groups (n=12). Crowns of all teeth in groups A–D were cut with diamond discs at a fixed distance of 3 mm from the incisal margin in a plane normal to the buccal surface. In all groups, coronal fragments were reattached to the remaining teeth by bonding with hybrid composite resin. After then, the teeth were restored to the following; group A, bonding and palatal laminate; group B, bonding and creation of a vertical groove; group C, bonding and creation of two slot grooves; group D, bonding only; and group E, intact tooth. It was lesser in group B than in groups C and E (p=0.007andp=0.006, resp.) and lesser in group D than in groups A, C, and E (p=0.002,p<0.001, andp<0.001, resp.). Within the limitations of this in vitro study, it can be concluded that methods employing palatinal laminate and small grooves are clinically feasible for the reattachment of tooth fragments to incisors.

Sealing Ability of ProRoot MTA and MTA-Angelus Simulating a One-Step Apical Barrier Technique- An in vitro Study

2009 ◽  
Vol 33 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Nikita Lolayekar ◽  
Sham Bhat ◽  
Sundeep Hegde
Keyword(s):  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Control Group ◽  
Blue Dye ◽  
Root Segments ◽  
Sealing Ability ◽  
Significant Difference ◽  
Group A ◽  
Group B

Endodontic treatment of the pulpless tooth with an immature root apex poses a special challenge for the clinician due to lack of an apical stop against which to compact an interim dressing of calcium hydroxide, or the final obturation material. A one-visit apexification protocol with MTA (Mineral Trioxide Aggregate) has be seen as an alternative to the traditional treatment practices with calcium hydroxide [Ca(OH)2]. The aim of this study was to investigate the use of MTA as a 5mm apical barrier by comparing sealing ability of ProRoot MTA to that of MTA-Angelus. 50 freshly extracted Maxillary and Mandibular single canal sound human incisor teeth were used in this study, which were decoronated to standardized 10mm root lengths. The root segments were prepared to simulate the clinical situation of an open apex with Gates Glidden burs # 5-1 and randomly assigned into 2 experimental groups of 20 samples each (Group A: ProRoot MTA, Group B: MTA-Angelus) and 10 root segments were used as control (Group C) where no apical barrier was used. After obturation the coronal portion of all samples were sealed with Glass Ionomer and stored at 37°C and 100% humidity for 4 weeks. The root segments were then double coated with nail varnish except for the apical 1mm and apical ends of all root segments were suspended vertically in methylene blue dye for 48 hours at room temperature. After removal from the dye, the samples were sectioned longitudinally and linear dye penetration was measured using a Grid and stereomicroscope. The measurements were tabulated and statistically analyzed. The mean percentage of dye leakage for Group A was 55.5% and for Group B was 53.25%. The results showed no statistically significant difference in the sealing ability of ProRoot MTA and MTA-Angelus when used as apical barriers.

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