scholarly journals Hydroxyl and calcium ions diffusion from endodontic materials through roots of primary teeth - in vitro study

2005 ◽  
Vol 13 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Ana Cristina Gerent Petry Nunes ◽  
Maria José de Carvalho Rocha
Keyword(s):  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Apical Surface ◽  
Working Length ◽  
Group A ◽  
Periapical Healing ◽  
Materials Used ◽  
Spectrometer Data

The purpose of this research was to evaluate the diffusion of calcium (Ca+2) and hydroxyl (OH-) ions from materials with a calcium hydroxide base - Ca(OH)2 through the intact roots of deciduous teeth. This diffusion of ions is important for periapical healing. Forty-six deciduous teeth were selected and instrumented to their working length with #40 files. The teeth were washed during cleaning and shaping with a 1% sodium hypochlorite (NaOCl) solution. The canals were dried with paper points. The teeth were divided into 4 groups based on the sealer type, with 10 specimens in each group. A fifth group of 6 teeth without sealer constituted the control group. The materials used as sealers were: Ca(OH)2 paste associated to propylene glycol (CaPE) thickened at the proportion of 2:1 w/v; UFSC (Federal University of Santa Catarina) paste - a mixture of 0.3g of zinc oxide with 0.3g of Ca(OH)2 with 0.2ml of olive oil 1:1 w/w; Vitapex® and Sealapex®. The coronal access was sealed with a glass ionomer after the root had been filled with each sealer. A one-third apical surface and foramen was hard-pressed with Araldite®. The teeth were stored individually in flasks containing saline solution at 37ºC and 100% humidity. The OH- and Ca+2 ions diffusion levels were determined using a pH meter and an atomic absorption spectrometer. Data were collected at 48 hours and at intervals of 7, 30, 45 and 60 days. Statistical analysis was performed using ANOVA to compare groups. In the pH evaluation, the CaPE group presented the largest OH- ions diffusion, which peaked at sixty days (p=0.0309), when compared to the other groups (p<0.0001). In relation to amount of Ca+2 ions released, the CaPE paste showed the best results, followed by the UFSC's paste. These results suggest that the CaPE paste was the material that allowed the highest diffusion of OH- and Ca+2 ions.

EVALUATION OF THE EFFECT OF FERROUS SULFATE ON ENAMEL DEMINERALIZATION OF HUMAN DECIDUOUS TEETH: AN IN VITRO STUDY

2017 ◽  
Vol 8 (3) ◽  
pp. 83-89
Author(s):  
Johnny Holanda De Gauw ◽  
Lara Maria Melo Costa ◽  
Rodrigo Neves Silva ◽  
Natanael Barbosa Santos ◽  
Maria Dânia Holanda Tenorio
Keyword(s):  
Ferrous Sulfate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Group A ◽  
Ph Cycling ◽  
Group B ◽  
Group D

Objective: This study aimed to evaluate the effect of ferrous sulfate (FS) on demineralized and non-demineralized human deciduous teeth. Additionally, it was evaluated the penetration extent of FS and its remineralizing effect on the enamel of deciduous teeth using Polarized Light Microscopy (PLM). Method: The sample comprised 44 human deciduous teeth. The 44 crowns were divided randomly into four groups: group A (FS after demineralization), group B (FS without demineralization), group C (only demineralization), and group D (control group). FS at 0.45 mol/L-1 was used daily (15 days) and demineralization was done by pH cycling (7 days). Then, three longitudinal slices of the crowns were photographed using PLM. The degree of penetration of the lesion or stain was measured in micrometers, as well as the distance between the external enamel surface and the core of lesion. Results: Group A showed a dark stain on the outer surface of enamel larger than the group B. It is suggested, a remineralizing effect when comparing groups, A and C. The mean depth and standard deviation for groups A, B, and C were 4.27µm (±1.49), 3.72 µm (±1.68) and 5.00 µm (±1.84), respectively. No dark stains were observed in group D. Conclusion: FS stained the demineralized and non-demineralized human deciduous teeth. However, dark stains in the non-demineralized teeth were smaller or absent, than in the demineralized teeth. Therefore, FS may have a protective effect against demineralization.

scholarly journals Erosive potential of three different commonly used pediatric syrups on deciduous teeth enamel: an in vitro study

2020 ◽  
Vol 7 (10) ◽  
pp. 2041
Author(s):  
Neha Thilak ◽  
Sundeep K. Hedge ◽  
Sham S. Bhat
Keyword(s):  
In Vitro Study ◽  
Titratable Acidity ◽  
Deciduous Teeth ◽  
Hardness Tester ◽  
Titrable Acidity ◽  
Group A ◽  
Group B ◽  
Post Hoc ◽  
Group D

Background: The aim of the study is to compare the erosive potential of three different commonly used pediatric syrups on deciduous teeth enamel. The objectives of the study were to assess the endogeneous pH and titratable acidity of mefenamic acid syrup (meftal P), cetrizine syrup (alerid) and multivitamin syrup (zincovit) and to evaluate the microhardness of the enamel after successive immersion cycles in each of the syrups.Methods: 40 non carious deciduous teeth were included for the study The samples were then randomly allocated into 4 groups (10 in each group): Group A- mefenemic acid syrup (meftal P), Group B- cetrizine syrup (alerid), group C- multivitamin syrup (zincovit) and group D- control (distilled water). The samples were then subjected to the immersion cycles in the syrups. Assessment of enamel surface microhardness was done using Vickers hardness tester at 7th day and 14th day. The pH and titrable acidity of the syrups were also assessed. One way analysis of variance (ANOVA) and post hoc tests were used for the statistical analysis.Results: Out of the test groups, group C showed the lowest pH of around 4.2 and exhibited the largest titrable acidity (22.8 ml) compared with 21 ml in group A and 15.5 ml in group B. At the end of 14th day, group A had microhardness of about 293.43.84±6.34, group B had 299.930±6.85, group C had 313.380±6.23 and group D had 334.190±5.51.Conclusions: All the pediatric liquid medications assessed in the study, meftal P, alerid and zincovit showed acidic pH, high titrable acidities and all the syrups showed loss of microhardness after exposure to the syrups for 14 days. Loss of microhardness was highest for meftal P followed by alerid and least for zincovit.

scholarly journals Effect of Honey in the Management of Otomycosis

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Amisha Patel ◽  
Vaghela D B
Keyword(s):  
Hearing Loss ◽  
Intervention Group ◽  
In Vitro Study ◽  
Signs And Symptoms ◽  
Control Group ◽  
Humid Climate ◽  
Group A ◽  
Clinical Control Trial ◽  
Group B

Otomycosis is a fungal infection of External ear, characterized by earache, pruritus, ear blockage, discharge, hearing loss and ringing in ear.  It is the most common clinical entity in the ENT clinics. The disease is more common in hot and humid climate. Its worldwide prevalence is 5.2 % and 9 % in India. 5-25 % of otitis externa cases are due to otomycosis. Bactericidal and fungicidal activity of Honey is proved previously by in vitro study. Honey has high osmolarity and acidic pH. And fungus does not grow in acidic media. With this background, present study is aimed to know the efficacy of the Honey in the management of Otomycosis. This randomized clinical control trial including two groups, one is intervention group (group A) and second is control group (group B). Pt is advised to instill three drops of Honey thrice a day for 7 days in Group A and three drops of Clotrimazole thrice a day for 7 days in Group B. There is statistically highly significant results was observed in all signs and symptoms except hearing loss which is significant in both Groups. Statistically insignificant difference was found between both Groups. 

scholarly journals Zeolite as a Bone Bio-Modifier Carrier: An In-Vitro Study

2019 ◽  
Vol 9 (1) ◽  
pp. 126
Author(s):  
Mobina Mousavi ◽  
Azadeh Esmaeil Nejad ◽  
Erfan Shamsoddin ◽  
Mohammad Mehdi Golabgiran ◽  
Behzad Houshmand
Keyword(s):  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Alizarin Red ◽  
Group A ◽  
Group B ◽  
And Function ◽  
Group D

Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear. Methods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1&mu;g/mL Z+A, Group B: 0.1&mu;g/mL Z without A, Group C: 0.2&mu;g/mL Z+A, Group D: 0.2&mu;g/mL Z without A, Group E: 0.3&mu;g/mL Z+A, Group F: 0.3&mu;g/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P&lt;0.05). Results: At 24 hours, results showed that solutions with 0.1&mu;g/mL, 0.2&mu;g/mL, and 0.3&mu;g/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p&lt;0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1&mu;g/mL, 0.2&mu;g/mL, and 0.3&mu;g/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p&lt;0.001). Conclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.

Lumbar spine stability following hemilaminectomy, pediculectomy, and fenestration

2000 ◽  
Vol 13 (04) ◽  
pp. 165-171 ◽  
Author(s):  
A. M. Lubbe ◽  
A. J. Guthrie ◽  
T. P. Hill
Keyword(s):  
Lumbar Spine ◽  
Lumbar Vertebrae ◽  
Bending Moment ◽  
In Vitro Study ◽  
Control Group ◽  
Lateral Bending ◽  
Angular Deformation ◽  
Group A ◽  
The Common

SummaryThe destabilising effect of the removal of a single vertebral diarthrosis and the ipsilateral incision of the anuliis fibrosus at two adjacent vertebrae in the canine lumbar spine was quantified in an in vitro study. Lumbar spine segments, from the first to fourth lumbar vertebrae, were harvested from canine cadavers and divided into 1 control group (A) and 3 experimental groups - hemilaminectomy and fenestration (B), pediculectomy and fenestration (C), fenestration alone (D). Samples were subjected to 4 point lateral bending to point of failure. Stiffness, maximum bending moment (Mbmax), angular deformation and bending moment at 15° (Mb at 15°) variables were determined for each group. Hemilaminectomy and fenestration had the greatest decrease in Mbmax, Mb at 15° and stiffness and the greatest increase in angular deformation. Pediculectomy and fenestration and the fenestration alone procedures caused similar and significant reductions in Mb at 15° and stiffness. Pediculectomy was less destabilizing than hemilaminectomy, although this difference was not statistically significant. Fenestration is the common and greatest destabilizing factor. The removal of a single pedicle and accessory process has minimal destabilizing effects.The destabilizing effect of hemilaminectomy, pediculectomy and fenestration were quantified and compared in a study using canine cadaver spines. The combination of hemilaminectomy and fenestration produced the greatest degree of instability, but fenestration was the shared, most significant, single destabilizing factor.

Sealing Ability of ProRoot MTA and MTA-Angelus Simulating a One-Step Apical Barrier Technique- An in vitro Study

2009 ◽  
Vol 33 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Nikita Lolayekar ◽  
Sham Bhat ◽  
Sundeep Hegde
Keyword(s):  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Control Group ◽  
Blue Dye ◽  
Root Segments ◽  
Sealing Ability ◽  
Significant Difference ◽  
Group A ◽  
Group B

Endodontic treatment of the pulpless tooth with an immature root apex poses a special challenge for the clinician due to lack of an apical stop against which to compact an interim dressing of calcium hydroxide, or the final obturation material. A one-visit apexification protocol with MTA (Mineral Trioxide Aggregate) has be seen as an alternative to the traditional treatment practices with calcium hydroxide [Ca(OH)2]. The aim of this study was to investigate the use of MTA as a 5mm apical barrier by comparing sealing ability of ProRoot MTA to that of MTA-Angelus. 50 freshly extracted Maxillary and Mandibular single canal sound human incisor teeth were used in this study, which were decoronated to standardized 10mm root lengths. The root segments were prepared to simulate the clinical situation of an open apex with Gates Glidden burs # 5-1 and randomly assigned into 2 experimental groups of 20 samples each (Group A: ProRoot MTA, Group B: MTA-Angelus) and 10 root segments were used as control (Group C) where no apical barrier was used. After obturation the coronal portion of all samples were sealed with Glass Ionomer and stored at 37°C and 100% humidity for 4 weeks. The root segments were then double coated with nail varnish except for the apical 1mm and apical ends of all root segments were suspended vertically in methylene blue dye for 48 hours at room temperature. After removal from the dye, the samples were sectioned longitudinally and linear dye penetration was measured using a Grid and stereomicroscope. The measurements were tabulated and statistically analyzed. The mean percentage of dye leakage for Group A was 55.5% and for Group B was 53.25%. The results showed no statistically significant difference in the sealing ability of ProRoot MTA and MTA-Angelus when used as apical barriers.

Enameloplasty effects on microleakage of pit and fissure sealants under load: an in vitro study

2000 ◽  
Vol 24 (4) ◽  
pp. 279-285 ◽  
Author(s):  
Christina Zervou ◽  
Gerald Kugel ◽  
Cataldo Leone ◽  
Athanasios Zavras ◽  
Eileen Doherty ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Conventional Technique ◽  
Control Group ◽  
Fissure Sealants ◽  
Occlusal Force ◽  
Pit And Fissure Sealants ◽  
Significant Difference ◽  
Group A ◽  
Group D

Enameloplasty has been implicated in the successful application of pit and fissure sealants. The present study aimed to evaluate the effect of enameloplasty technique on microleakage of sealants when occlusal force was applied on the teeth. The study also allowed a direct comparison of the effectiveness of enameloplasty, when two different burs were used. Six groups of ten human extracted wisdom teeth were tested. Group A: no load, conventional (no enameloplasty - control); Group B: no load, enameloplasty 1/2 round bur (Brasseler USA); Group C: no load, enameloplasty diamond fissure bur REF/UP 791( Ultradent); Group D: load 500 N, conventional , Group E: load 500 N, 1/2 round bur; Group F: load 500 N, diamond fissure bur. Specimens were thermocycled for 500 cycles at 5° and 55° with a dwell time of 30 seconds after load application. Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underling fissure. The Kruskal-Wallis One Way Anova and Mann -Whitney U test showed group D having the greatest degree of microleakage statistically significant (p&lt;0.05) comparing with other groups. Sealants prepared with the conventional technique (A, D) had statistically significant differences in microleakage with those prepared with enameloplasty (B,C,E, F) (p = 0.01). Enameloplasty in groups with no load (B, C) did reduce microleakage, but not significantly (p = 0.3) However, loaded teeth with enameloplasty appeared to perform superior, as compared to those without (p=0.005) Regarding the effect of load, teeth without load (Groups A,B, C) were found to have significantly less microleakage than teeth where mechanical force had been applied (Groups D, E, F), (p = 0.01). Microleakage in Group A (conventional technique without load) was significantly less than microleakage in Group D (conventional technique with load), (p = 0.04). However, load did not seem to influence microleakage when enameloplasty had been performed. This finding was true for both round bur enameloplasty (p = 0.29), and fissure bur enameloplasty (p = 0.26). There was no statistically significant difference between Groups B, E (round bur) and C, F (fissure bur) (p &gt; 0.05), or between Groups B and C (p &gt; 0.05) and between Groups E and F (p &gt; 0.05). The results of the study indicated that enameloplasty reduced microleakage of pit and fissure sealants, especially when load was applied to teeth, irrespective of what bur was used to enlarge the fissure, as there was no statistical significant differences between the round and fissured diamond burs. The application of occlusal force to the tooth produces significantly more microleakage, unless enameloplasty is performed.

scholarly journals Effect of Laser Activated Sodium Hypochlorite on Sealing Ability of Two Endodontic Sealers Using Stereomicroscope-An In vitro Study

2021 ◽  
Vol 6 (3) ◽  
Author(s):  
Kiranmayi Govula
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Sealing Ability ◽  
Working Length ◽  
Ah Plus ◽  
Endodontic Sealers

Introduction: The combination of various irrigants, irrigation techniques along with root canal sealers helps in achieving a three-dimensional seal in root canals, which is of clinical relevance. The present in-vitro study aims to assess and compare the efficacy of laser-activated NaOCl on the sealing ability of two endodontic sealers. Materials and Methods: Forty extracted human single-rooted permanent teeth were collected and decoronated 12 mm from their apices. Instrumentation did with an 11 mm working length using Rotary Pro taper files to F3. All the teeth grouped into four (n= 10) based on irrigation protocol and sealer used as follows: Group A1 – Laser activated NaOCl + AH Plus Group A2 – Laser activated NaOCl +nano seal, Group B1 – NaOCl + AH Plus, Group B2 – NaOCl + nano seal. The lateral condensation technique did obturation. Roots stored in freshly prepared 1% methylene blue for 72 hours. The roots were split longitudinally along the long axis using a diamond disc underwater coolant, and markings made at 2, 4, and 6 mm from the apex. The depth of dye penetration and microleakage examined under a stereomicroscope (Magnus) at 30× magnification. Results: Statistical analysis carried out by using the software, statistical package for social sciences [SPSS]. Results represented in the form of minimum, mean, and standard deviation. Conclusion: The laser-activated NaOCl group exhibited better sealing ability than the control group. Among the two sealers, the Bio root RCS sealer showed less leakage & better sealing ability than AH plus Clinical Significance: Increasing the temperature of low concentration sodium hypochlorite solution by the action of the diode laser, not only enhances its immediate tissue dissolution capacity but also increased the dentin permeability by removing the smear layer. Further leading to better adhesion between the root canal sealer and the gutta-percha with the radicular dentin.

Caries protective effect of two Self-etch dentin bonding agents on denuded root dentin surface: In Vitro Study

2019 ◽  
Vol 11 (2) ◽  
pp. 36-41
Author(s):  
Dr. Jini P M ◽  
Dr. Shibani Shetty ◽  
Dr. Jayalakshmi K B ◽  
Dr. Prasannalatha Nadig ◽  
Dr. Sujatha I
Keyword(s):  
Protective Effect ◽  
In Vitro Study ◽  
Bonding Agent ◽  
Control Group ◽  
Bonding Agents ◽  
Dental Adhesive ◽  
Dentin Bonding ◽  
Group A ◽  
Root Dentin

Objective: To evaluate the caries protective effect of two self-etching dental adhesive agents on the denuded root surface. Materials and methods: The roots of 30 freshly extracted , non-carious human premolars were cleaned and denuded of cementum. The teeth surfaces were covered with acid-resistant nail varnish, exposing two rectangular windows (lingual and buccal surface) of 4 x 4 mm each. The window on the lingual furface served as the control , while the buccal window were treated with dentin bonding agent. All specimens were store in acidifed gel for 6 days for demineralization. The specimens were then divided among two experimental groups-Group A: Optibond all in one and Group B: Bond-1SF.In Group C (control group) bonding agent was not applied. The specimens were stored in 0.9% sodium chloride solution for 14 days. Plano parallel tooth sections (80 ± 20 μm) were obtained. Tooth sections were evaluated for caries‑like demineralized zone under a polarized microscope. The values obtained were analyzed using one‑way ANOVA and Tukey honesty tests. Results: In the Control group the mean depth of demineralization was 80.08µm .The depth of demineralization was significantly reduced in Group A and B. Conclusion: Self-etching dental adhesive agents with and without solvent is an effective method to prevent demineralization of root dentin.

Reverse torque values of abutment screws after dynamic loading: The effects of sealant agents and the taper of conical connections

2020 ◽  
Author(s):  
Arda Ozdiler ◽  
suleyman dayan ◽  
Burc Gencel ◽  
Gulbahar Isık-Ozkol
Keyword(s):  
Dynamic Loading ◽  
Antibacterial Agent ◽  
In Vitro Study ◽  
Control Group ◽  
Silicone Sealant ◽  
Reverse Torque ◽  
The Stability ◽  
Torque Values ◽  
Chlorhexidine Gel

This in vitro study evaluated the influence of taper angles on the internal conical connections of implant systems and of the application of chlorhexidine gel as an antibacterial agent or a polyvinyl siloxane (PVS) sealant on the reverse torque values of abutment screws after dynamic loading. The current study tested four implant systems with different taper angles (5.4°, 12°, 45°, and 60°). Specimens were divided into three groups: control (neither chlorhexidine gel filled nor silicone sealed), 2% chlorhexidine gel-filled or silicone-sealed group, and group subjected to a dynamic load of 50 N at 1 Hz for 500,000 cycles prior to reverse torque measurements. Quantitative positive correlation was observed between the taper angle degree and the percentage of tightening torque loss. However, this correlation was significant only for the 60° connection groups except in the group in which a sealant was applied ( p = 0.013 for the control group, p = 0.007 for the chlorhexidine group). Percentages of decrease in the torque values of the specimens with silicone sealant application were significantly higher compared with both the control and chlorhexidine groups ( p = 0.001, p = 0.002, p = 0.001, and p = 0.002, respectively, according to the increasing taper angles); the percentage of decrease in torque values due to chlorhexidine application was statistically insignificant when compared with the control group. The application of gel-form chlorhexidine as an antibacterial agent does not significantly affect the stability of the implant–abutment connection under dynamic loads. PVS sealants may cause screw loosening under functional loads.

Sign in / Sign up

Export Citation Format

Share Document