Thalassaemia genes in Baghdad, Iraq

To estimate the prevalence of thalassaemia genes in Baghdad, a study was made of 502 randomly selected pregnant women attending a major maternity care clinic in the city. A reduced mean corpuscular volume [MCV] of < 80 fl was used as a screening test for thalassaemia in this population, followed by a battery of tests, including haemoglobin analysis and iron studies, to confirm the diagnosis. Using this approach, 22 cases were diagnosed of beta-thalassaemia trait, five cases of alpha-thalassaemia trait and one case of delta beta-thalassaemia trait. Based on these figures the estimated prevalence rates of beta-, alpha- and delta beta-thalassaemia traits in Baghdad, would be 4.4%, 1.0% and 0.2%, respectively. The study also includes a discussion of the above findings in the context of those reported in other neighbouring countries

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IRON DEFICIENCY AND THALASSAEMIA

The Professional Medical Journal ◽

10.29309/tpmj/18.4672 ◽

2018 ◽

Vol 25 (12) ◽

pp. 1863-1868

Author(s):

Asma Shaikh ◽

Nadeem Nusrat ◽

Muhammad Akbar Agha ◽

Asma Shabbir

Keyword(s):

Iron Deficiency ◽

Vitamin B12 ◽

Mean Corpuscular Volume ◽

Complete Blood Count ◽

Vitamin B12 Deficiency ◽

Reference Laboratory ◽

Corpuscular Volume ◽

Beta Thalassaemia ◽

Thalassaemia Trait ◽

B12 Deficiency

Objectives: To study the importance of normal or low mean corpuscular volume in vitamin B12 deficiency due to co-existence of iron deficiency or beta thalassaemia trait masking a rise in mean corpuscular volume. Study Design: Observational non-probability cross sectional study. Setting: DDRRL. Period: January 2014 to September 2014. Methods: 105 vitamin B12 deficient cases (vitamin B12 less than 200ng/l) who presented with normal or low mean corpuscular volume (MCV less than 95 fl) on complete blood count (CBC) were determined from Dow diagnostic research and reference laboratory (DDRRL). Serum ferritin, red blood cell folate (RBC Folate) level and Hemoglobin electrophoresis for beta thalassaemiatrait were analysed in these patients. Results: Total of 105 vitamin B12 deficient patients who fulfilled the inclusion criteria were enrolled in this study from which 39 (37.14%) were male and 66 (62.85%) were females. Amongst them 36.19% had microcytosis with the mean age of 37±16.2 years while 63.8% were normocytic with mean age of 41.58±15.65 years. In microcytic group, iron deficiency, beta thalassaemia trait, combined deficiency of B12, iron and beta thalassaemia trait and RBC folate deficient were 52.6%,34.21%, 7.8% and 2.63% respectively. In normocytic group, iron deficiency, beta thalassaemia trait and RBC Folate deficient were 13.4%, 00% and 11.9% respectively. Conclusion: There is a significant coexistent frequency of iron deficiency and beta thalassaemia trait in vitamin B12 deficiency with normal or low MCV especially in females of 20-40 years of age. There should be a high index of suspicion for B12 deficiency when investigating anaemia with normal or altered red cell indices.

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Effect of alpha thalassaemia trait and enhanced gamma chain production on disease severity in beta thalassaemia major and intermedia.

Archives of Disease in Childhood ◽

10.1136/adc.70.1.30 ◽

1994 ◽

Vol 70 (1) ◽

pp. 30-34 ◽

Cited By ~ 16

Author(s):

P Gringras ◽

B Wonke ◽

J Old ◽

A Fitches ◽

D Valler ◽

...

Keyword(s):

Disease Severity ◽

Thalassaemia Major ◽

Gamma Chain ◽

Beta Thalassaemia ◽

Thalassaemia Trait ◽

Alpha Thalassaemia

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Value of Mean Corpuscular Volume and Mean Corpuscular Haemoglobin in Screening for β-Thalassaemia Trait

Acta Haematologica ◽

10.1159/000094688 ◽

2006 ◽

Vol 116 (3) ◽

pp. 223-225 ◽

Cited By ~ 3

Author(s):

Manju Mamtani ◽

Anil Jawahirani ◽

Vinky Rughwani ◽

Kishor Das ◽

Hemant Kulkarni

Keyword(s):

Mean Corpuscular Volume ◽

Corpuscular Volume ◽

Corpuscular Haemoglobin ◽

Thalassaemia Trait ◽

Mean Corpuscular Haemoglobin

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Evaluation of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Screening and Diagnosis of Beta Thalassaemia Trait in Pakistani Population

Pakistan Journal of Medicine and Dentistry ◽

10.36283/pjmd10-1/002 ◽

2021 ◽

Keyword(s):

Screening Test ◽

Complete Blood Count ◽

Osmotic Fragility ◽

Rapid Screening ◽

Red Cell ◽

Pakistani Population ◽

Single Tube ◽

Beta Thalassaemia ◽

Naked Eye ◽

Thalassaemia Trait

Background: Beta thalassaemia is an inherited disease that is a cause of continuous burden on the affected families and the society. The objective of the study was to evaluate the efficacy of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) for the detection of beta thalassaemia trait when used for the screening in Pakistani population. Methods: All participants requesting a complete blood count were included in the study. Informed consent was obtained. Naked Eye Single Tube Red Cell Osmotic Fragility Test was performed with freshly prepared 0.36% buffered saline. Turbidity in the tubes was an indication for individuals to be positive for beta thalassaemia trait. Hemoglobin electrophoresis was performed on all positive cases to confirm the sensitivity and the reliability of the screening test. Chi-Square test was used to test for any significant correlation between the different hematological parameters and other variables. Results: A total of 812 patients were included in the study from January 2017 to June 2017. The mean age of participants was 35.5 + 13.6 years. There were 351 (43.2%) male and 461 (56.7%) female participants. A 100% Naked Eye Single Tube Red Cell Osmotic Fragility Test sensitivity was obtained with 65 (8%) positive cases having HbA2 levels of more than 3.2% indicating their true positivity whereas, all 747 (92%) negative cases having HbA2 levels of less than 3.2% indicating their true negativity. Conclusion: NESTROFT was found and a rapid screening test for the identification of carriers of beta thalassaemia trait in our population.

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Antenatal thalassaemia screening – determining the appropriate mean corpuscular volume (MCV) cut-off for excluding alpha thalassaemia

Pathology ◽

10.1097/01.pat.0000461461.72644.f7 ◽

2015 ◽

Vol 47 ◽

pp. S57

Author(s):

Koon Lee ◽

Carol Cheung

Keyword(s):

Mean Corpuscular Volume ◽

Corpuscular Volume ◽

Alpha Thalassaemia

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Prevalence of anaemia and it's associated risk factors among under five children at Mukinge Missions Hospital in Kasempa District: Retrospective Study (Preprint)

10.2196/preprints.16826 ◽

2019 ◽

Author(s):

Lloyd Sampa

Keyword(s):

Risk Factors ◽

Retrospective Study ◽

Mean Corpuscular Volume ◽

Sub Saharan Africa ◽

Corpuscular Volume ◽

Under Five ◽

Under Five Children ◽

Mission Hospital ◽

Associated Risk Factors ◽

Prevalence Of Anemia

BACKGROUND Anemia is a worldwide major problem known to affect people throughout the world. It has an adverse effect on both the social and economic development. The worldwide prevalence of anemia is 9% in developed nations. The global estimate indicates that 293.1 million of children under five years, approximately 43%, are anaemic worldwide and 28.5% of these children are found in sub Saharan Africa. In Zambia specifically Kasempa, no documented studies on prevalence have been done. Despite iron supplementation being given to pregnant women and the availability of blood transfusion. The burden of the disease remains high as determined by high mortality and morbidity. This study aims at determining the prevalence of anemia and the associated risk factors among under-five children at Mukinge Mission Hospital in Kasempa District. Knowledge of prevalence and the associated risk factors of anaemia will enhance early detection and timely management. OBJECTIVE 1.To determine the hemoglobin status of anaemia by its severity among anaemic under-five children admitted at Mukinge Mission Hospital. 2.To assess the association of anaemia with Malaria among under-five children admitted at Mukinge Mission Hospital. METHODS This was a retrospective study review of under-five children that were diagnosed and managed of Anemia at Mukinge Missions Hospital, over the period of period of 2015, 2017 and 2018. .Data of the variables of interest was extracted and analyzed using SPSS. RESULTS A sample population of 52 children was included in our study. The majority of the children were females 28 (53.8 %) and 24 (46.2 %) were Males. It was found that moderate and severe anaemia was 17.3% and 82.7 % respectively. Additionally, Majority of the anaemic children (75%) had Normocytic anaemia. The Pearson Chi square test revealed no statistical relationship between the variables; Malaria (p=0.58), Age (P=0.82), Gender (P=0.91). CONCLUSIONS According to our study, 39 (75%) had normal mean corpuscular volume which could suggest chronic diseases and sickle cell anemia. 11 (21.2%) had a low mean corpuscular volume indicating Microcytic anemia which could suggest diseases such as iron deficiency and thalassemia among many other causes. However, we were unable to determine the specific cause of anemia.

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Vitamin D and Blood Parameters

Biomolecules ◽

10.3390/biom11071017 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1017

Author(s):

Thomas Müller ◽

Lutz Lohse ◽

Andreas Blodau ◽

Katja Frommholz

Keyword(s):

Vitamin D ◽

Mean Corpuscular Volume ◽

Plasma Concentrations ◽

Blood Parameters ◽

Vitamin D Supplementation ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Volume ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin ◽

The Impact

Background: Vitamin D has a steroid- and an anabolic-resembling chemical structure. Vitamin D is essential for many processes in the human body after hydroxylation. Aims of the Study: To investigate the impact of 25-hydroxy-vitamin D plasma concentrations on the blood parameters number of erythrocytes, hematocrit, mean corpuscular hemoglobin and mean corpuscular volume. Methods: Serial assessments were done in 290 patients with multiple sclerosis and repeated after a mean interval of 245 days. A recommendation for vitamin D supplementation was given in case of a concentration lower than 20 ng/mL combined with a prescription of a formulation containing vitamin D but not vitamin K. Results: There was a fall of vitamin D in 119 subjects and a rise in 164, while no change appeared in 7 participants. When vitamin D values went down between both assessments moments, the computed increase of mean corpuscular haemoglobin was significantly lower compared with the rise of mean corpuscular haemoglobin associated with a vitamin D elevation. When vitamin D declined, the computed fall of mean corpuscular volume fall was significantly lower compared with the decrease of mean corpuscular volume, when vitamin D rose. Positive correlations were found between differences of vitamin D and mean corpuscular haemoglobin, respectively mean corpuscular volume. Inverse relations appeared between disparities of vitamin D and erythrocytes, respectively haematocrit. Conclusions: The elevation of vitamin D plasma levels provides enhanced preconditions for a better tissue oxygenation on a cellular level.

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Inaccuracy in automated measurement of hematocrit and corpuscular indices in the presence of severe hyperglycemia

Blood ◽

10.1182/blood.v57.6.1065.1065 ◽

1981 ◽

Vol 57 (6) ◽

pp. 1065-1067 ◽

Cited By ~ 21

Author(s):

JA Strauchen ◽

W Alston ◽

J Anderson ◽

Z Gustafson ◽

LF Fajardo

Keyword(s):

Blood Glucose ◽

Mean Corpuscular Volume ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Volume ◽

Automated Measurement ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Hyperosmolar Glucose

Abstract Because we recently observed two patients with severe diabetic hyperglycemia and spuriously elevated electronically determined hematocrit and mean corpuscular volume (MCV), we investigated the effect of hyperglycemia on two popular automated hematology systems, the Coulter S and Ortho ELT-8. Marked hyperglycemia (blood glucose 800-- 2000 mg/dl) caused consistent overestimation of the electronically determined MCV compared to that derived from a simultaneous spun microhematocrit. The resultant overestimation and underestimation, respectively, of the derived values for hematocrit and mean corpuscular hemoglobin concentration may be clinically misleading. The mechanism of MCV elevation in hyperglycemia appears to be swelling of hyperosmolar glucose “loaded” erythrocytes when diluted into “isotonic” counting medium. This effect is readily circumvented by determination of a spun microhematocrit.

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