scholarly journals Long- term effect of melatonin on submandibular salivary glands in old rats

1998 ◽  
Vol 4 (2) ◽  
pp. 324-331
Author(s):  
M. A. Ashour
Keyword(s):  
Electron Microscopy ◽  
Salivary Glands ◽  
Light And Electron Microscopy ◽  
Degenerative Changes ◽  
Control Group ◽  
Cellular Activity ◽  
Long Term Effect ◽  
Submandibular Salivary Glands ◽  
Old Rats

The effect of melatonin on the submandibular salivary glands of old rats was studied using 20 control and 20 experimental rats which had received melatonin daily for 5 months. The glands were first weighed and then processed for light and electron microscopy. The glands of the melatonin rats were significantly heavier than the controls. With light microscopy, the control group showed a loss of normal architecture of the acini and multiple degenerative changes whereas in the melatonin group the acini had clear architecture and few degenerative changes. With electron microscopy, the control group again showed degenerative changes and little cellular activity whereas the melatonin group had features which indicated increased cellular activity

scholarly journals The istant changes of the salivary glands to stress

2010 ◽  
Vol 9 (1) ◽  
pp. 31-35
Author(s):  
A. V. Gerasimov ◽  
S. V. Logvinov ◽  
V. P. Kostyuchenko
Keyword(s):  
Electron Microscopy ◽  
Salivary Glands ◽  
Guinea Pigs ◽  
Light And Electron Microscopy ◽  
Secretory Activity ◽  
Duct Cell ◽  
Cell Stress ◽  
Microwave Exposure ◽  
Submandibular Salivary Glands ◽  
Granular Ducts

Night lighting and microwave exposure have been influence on structures of stress realization. The endocrine, fotoperiodical and adaptive functions of rodent submandibular salivary glands belonging to hormone produced duct cells. To evaluate their morphofunctional state at guinea-pigs and rats using the methods of light and electron microscopy there have been analyzed striated and granular ducts. It has been revealed that instant and twenty-four-hour strengthening of duct cell stress induced secretory activity was similar. It is concluded that salivary glands take part in circadian expectations to stress.

scholarly journals Lichtenstein inguinal hernia repairs with porcine small intestine submucosa: a 5- year follow-up. a prospective randomized controlled study

2021 ◽  
Author(s):  
Baoshan Li ◽  
Xin Zhang ◽  
Yi Man ◽  
Jiadong Xie ◽  
Wei Hu ◽  
...  
Keyword(s):  
Chronic Pain ◽  
Inguinal Hernia ◽  
Hernia Repair ◽  
Term Effect ◽  
Control Group ◽  
Small Intestine Submucosa ◽  
Long Term Effect ◽  
Porcine Small Intestine Submucosa

Abstract Porcine small intestine submucosa (SIS) biologic patch has been used in inguinal hernia repair. However, there are little data available to assess the long-term effect after repair. This study aimed to explore the long-term effect of SIS patch in open inguinal hernia repair. Sevent-six patients with unilateral inguinal hernia were treated with Lichtenstein tension-free hernia repair using SIS patch (Beijing Datsing Bio-Tech Co., Ltd.) and Surgisis patch (COOK, USA) in Tianjin Union Medical Center and China-Japan Friendship Hospital. In the trial, the long-term efficacy of the treatment group and the control group were compared. A total of 66 patients in both groups received long-term follow-up (> 5 years) after surgery, with a follow-up rate of 86.8%. During the follow-up period, there was one case of recurrence, one case of chronic pain in the control group. There was no statistically significant difference (P > 0.05) in terms of recurrence, chronic pain, foreign body sensation and infection between the two groups of patients. After long-term observations, it has been found that the porcine small intestinal submucosa (SIS) biological patch is safe and effective for inguinal hernia Lichtenstein repair, and has a low recurrence rate and complication rate.

scholarly journals Morphological changes of gastric and colonic mucosa in rats of different ages under the action of nanocrystalline cerium dioxide

2014 ◽  
Vol 68 (3) ◽  
pp. 46-50
Author(s):  
O. Iefimenko ◽  
O. Savchenko ◽  
T. Falalyeyeva ◽  
O. Kyric ◽  
M. Spivak
Keyword(s):  
Cerium Dioxide ◽  
Plasma Cells ◽  
Morphological Changes ◽  
Degenerative Changes ◽  
Control Group ◽  
Gastric Glands ◽  
Different Ages ◽  
Nanocrystalline Cerium Dioxide ◽  
Old Rats ◽  
Differentiation And Proliferation

We have studied the effect of nanocrystalline cerium dioxide (NCD) on the morphological state of the gastric mucosa and colon in rats of different ages. It was found the degenerative changes and dysregeneration (violation the ratio of value of major and parietal cells), atrophic or hyperplastic changes. NCD restored the processes of differentiation and proliferation of epithelial cells of gastric glands. In the control group of old rats mucosa of the colon was focal thinner, the cells had degenerative changes, it was observed the change in nuclear-cytoplasmic ratio of cells, were found foci of infiltration of lymphocytes, macrophages, plasma cells. NCD in old rats caused a decrease in the number of cells in a state of degeneration and apoptosis, increased proliferative activity of cells increased the number of goblet cells. Thus, NCD restored morpho-functional structure of the mucous of the stomach and colon.

Limitations of In-Vitro Labeling of Endothelial Cells with Indium-111 Oxine

1995 ◽  
Vol 4 (3) ◽  
pp. 291-296 ◽  
Author(s):  
H.M.H. Carr ◽  
J.V. Smyth ◽  
O.B. Rooney ◽  
P.D. Dodd ◽  
H. Sharma ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Attachment ◽  
Control Group ◽  
Vascular Prostheses ◽  
Long Term Effect ◽  
Indium 111 ◽  
Suitable Technique

Indium-111 oxine labeling is widely used as a marker of endothelial cell attachment to vascular prostheses. The long term effect of labeling human adult endothelial cells (HAECs) with this isotope has not been determined. In this study the viability of labeled HAECs, leakage of isotope from labeled cells and adherence of circulating isotope to fibronectin coated prostheses were investigated over 24 h. The effect of incubation time on labeling efficiency was also assessed. There were significant differences in cell viability between the labeled and unlabeled groups beyond 4 h (p < 0.005, 2-tailed, unpaired t-test). In the control group cell numbers increased by 42% while in the labeled group this had decreased by 20% at 24 h. Spontaneous leakage increased with time but was maximal in the first 2 h. Adherence of circulating isotope to fibronectin coated expanded polytetrafluoroethylene (ePTFE) grafts was minimal but was significantly greater to gelatin impregnated Dacron (GEL-SEAL) beyond 1 hour (p < 0.05). Incubation times greater than 5 minutes during labeling do not significantly improve labeling efficiency, and may contribute to toxicity by prolonging exposure to oxine. Indium-111 oxine labeling of HAECs is a suitable technique for acute studies of endothelial cell kinetics up to 4 h, but its use in chronic studies may lead to significant underestimations of cell retention.

Efficacy assessment of radiosynoviorthesis on the progression of radiological osteoarthritic features of hip and ankle joint in patients with osteoarthritis and rheumatoid arthritis

2020 ◽  
Vol 59 (03) ◽  
pp. 269-275
Author(s):  
Imre Szerb ◽  
Tamás Gál ◽  
Dániel Kiss ◽  
Viktória Nagy ◽  
László Hangody
Keyword(s):  
Rheumatoid Arthritis ◽  
Ankle Joint ◽  
Term Effect ◽  
Control Group ◽  
Hip Joints ◽  
Study Objective ◽  
Long Term Effect ◽  
Structural Progression ◽  
Ankle Joints

Abstract Objective The study objective was to evaluate the long-term effect of radiosynoviorthesis (RSO) on the progression of radiological and nuclear-medical osteoarthritic features of hip and ankle joint in patients with osteoarthritis and rheumatoid arthritis. Patients and Methods The study included 89 hip joints of 81 patients, of which 48 had osteoarthritis (OA) and 33 had rheumatoid arthritis (RA). In terms of ankle joints, 64 patients were included of which 43 suffered from OA and 21 from RA. The mean follow-up time was 9.2 years for OA and 8.9 years for RA patients. Patients with RA were the active control group. Structural alterations of the hip joints were evaluated following Kellgren-Lawrence score and of ankle joint following Takakura score for both disease entities. For the evaluation of synovitis 2-phase bone scintigraphy was performed. Results RSO could prevent radiologic status deterioration among 70.6 % of hip osteoarthritic patients and 73.7 % among the hip RA patients. No structural progression was observed in 79.1 % of the treated ankle joints in patients with OA and in 85.7 % of the ankle joints in patients with RA.The scintigraphic response rate for the hip joints of OA patients was 68.6 %, 76.3 % for hip joints of RA patients, 83.1 %, ankle joints of OA patients, and 90.4 % ankle joints of RA patients.The radiographic and scintigraphic RSO response rates were not significantly higher for both joints in RA than OA patients. Conclusion RSO can be a good alternative in the treatment of synovitis and prevention of deterioration of inflammatory and radiographic features even in patients suffering from osteoarthritis and rheumatoid arthritis. RSO may have also a long-term effect to stop or delay progression of both diseases.

scholarly journals Objective Structured Assessment of Technical Skills to Teach and Study Retention of Fourth-Degree Laceration Repair Skills

2015 ◽  
Vol 7 (1) ◽  
pp. 32-35 ◽  
Author(s):  
Anjali Martinez ◽  
Caroline Cassling ◽  
Jennifer Keller
Keyword(s):  
Intervention Group ◽  
Technical Skills ◽  
Obstetrics And Gynecology ◽  
Control Group ◽  
Fourth Degree ◽  
Written Examination ◽  
Long Term Effect ◽  
Baseline Knowledge ◽  
Educational Workshop

Abstract Background Fourth-degree perineal lacerations are a serious but infrequent complication of childbirth. Objective We studied the long-term effect of an educational workshop on the knowledge and ability of obstetrics and gynecology residents to repair fourth-degree lacerations. Methods We assessed obstetrics and gynecology residents' baseline knowledge and skill of fourth-degree laceration repair by using a written examination and the Objective Structured Assessment of Technical Skills (OSATS). After the educational intervention (a lecture, a demonstrational video, and practice on a model), residents completed a written and OSATS posttest. Six months later, residents took the same posttests to determine their level of retention. Another group of residents who had not attended the workshop also took the tests at the 6-month mark and served as a control group. Results A total of 17 residents were in the intervention group and 11 residents in the control group. The pretest written examination mean was 6.1/10 and the OSATS mean was 10.9/18. After the workshop, the written mean increased to 9.1/10 and the OSATS to 16.6/18. This improvement was statistically significant (P &lt; .01). Compared to the pretest, the 6-month follow-up scores had a statistically significant increase (written mean, 8.0/10, P &lt; .01, and OSATS mean 15.5/18, P &lt; .01). Conclusions Residents improved on the written examination and OSATS after the educational workshop and maintained this improvement for 6 months. This intervention may prepare graduating residents for repairing future fourth-degree lacerations they may not have encountered during training.

Mouse ovarian tissue vitrification on copper electron microscope grids versus slow freezing: a comparative ultrastructural study

2015 ◽  
Vol 27 (7) ◽  
pp. 1020 ◽  
Author(s):  
Ferda Topal-Celikkan ◽  
Sinan Ozkavukcu ◽  
Deniz Balci ◽  
Sibel Serin-Kilicoglu ◽  
Esra Atabenli-Erdemli
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Cancer Therapy ◽  
Ovarian Tissue ◽  
Light And Electron Microscopy ◽  
Slow Freezing ◽  
Ovarian Tissue Cryopreservation ◽  
Control Group ◽  
Primordial Follicles ◽  
User Friendly

There are many reasons, including cancer therapy, for premature ovarian failure and infertility. Oocyte, embryo and ovarian cryopreservation are current options for fertility preservation. Ovarian tissue cryopreservation is essential in patients whose cancer therapy cannot be delayed, including prepubertal girls, and is mostly performed using slow freezing. In the present study, mouse ovarian tissues were vitrified on copper electron microscope grids (n = 18) or conventionally slow frozen (n = 18). Post-thaw tissues were examined histologically using light and electron microscopy and compared with the control group. According to light microscopy observations, antral follicles were found to be better preserved with the slow freezing technique rather than vitrification. Electron microscopy revealed swollen mitochondria in the oocyte cytoplasm, condensations in the zona pellucida, breakages in the junctions of granulosa cells and vacuolisation in the extracellular space in pathologic follicles, which were relatively more frequent, in the vitrification group after thawing. These results indicate that ovarian slow freezing is preferable than vitrification on copper electron microscope grids, especially for larger follicles. Conversely, vitrification of ovarian pieces using cooper grids is user-friendly and provided good protection for primordial follicles and stromal cells. There is a need for further studies into advanced tissue vitrification techniques and carriers.

Maintenance of differentiated sheep thyroid cells in primary culture for three months

1984 ◽  
Vol 107 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Carmel Mothersill ◽  
C. Seymour ◽  
J. F. Malone
Keyword(s):  
Electron Microscopy ◽  
Sudden Death ◽  
Primary Culture ◽  
Light And Electron Microscopy ◽  
Functional Differentiation ◽  
Entire Period ◽  
Iodine Uptake ◽  
Long Term Effects ◽  
Thyroid Cells

Abstract. A method is described which permits culture of primary thyroid cells without subculture for at least 100 days. Cultures are maintained without medium changes for the entire period, and concentrated glucose is added to replenish energy supplies at carefully defined intervals. The cells retain morphological and functional differentiation shown by light and electron microscopy, PAS positive histochemistry, iodine uptake and T4 production for at least 100 days. After this time fairly sudden death of the cultures occurs. Possible mechanisms for the effect are postulated. The technique should make it possible to study long-term effects of drugs/radiation on differentiated cultures without the need for continuous subculture.

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