scholarly journals Clinical and Biochemical Assessment of Lycopene Gel Combined With Nanohydroxyapatite Graft in Treatment of Grade II Furcation Defects: A Randomized Controlled Clinical Study

Perio J ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 28-37
Author(s):  
Dalia Shawky Gaber ◽  
Nevine Hassan Kheir El Din ◽  
Hamdy Ahmed Nassar ◽  
Mostafa Saad El-Din Ashmawy ◽  
Ola Mohamed Ezzatt
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Parameters ◽  
Clinical Attachment Level ◽  
Randomized Controlled ◽  
Furcation Defects ◽  
Grade Ii ◽  
Crevicular Fluid ◽  
Controlled Clinical Study ◽  
Open Flap Debridement ◽  
Attachment Level

Background: This study aimed to evaluate the effects of lycopene gel, as a natural antioxidant, mixed with a nanohydroxyapatite graft (NHG) covered by an occlusive resorbable collagen membrane (CM) in the surgical treatment of grade II furcation defects and on the gingival crevicular fluid (GCF) levels of 8-hydroxydeoxyguanosine (8-OHdG), as a marker of oxidative injury. Methods: In this randomized controlled clinical study a total of 24 patients with grade II furcation defects were randomly assigned into three equal groups. Furcation defects in group I were managed with lycopene gel mixed with NHG and CM, group II with NHG and CM, and group III with open flap debridement only. Site-specific changes in clinical parameters including probing depth (PD), vertical clinical attachment level (VCAL), horizontal clinical attachment level (HCAL), radiographic maximum vertical depth (MAX V), and maximum horizontal depth (MAX H) were measured at baseline and six months postoperatively. Gingival crevicular fluid levels of 8-OHdG were analyzed using enzyme-linked immunosorbent assay (ELISA) at baseline, one week, and three months. Results: Surgical management of grade II furcation defects resulted in a significant reduction in PD and 8-OHdG levels and a gain in CAL, MAX V, and MAX H in all groups. The differences between lycopene treated sites compared to NHG and CM alone were not significant at six months but demonstrated significantly superior clinical parameters compared to open flap debridement alone. Conclusion: Lycopene does not confer a benefit when combined with NHG in the surgical treatment of grade II furcation defects.

scholarly journals Effect of Periodontal Surgery on Osteoprotegerin Levels in Gingival Crevicular Fluid, Saliva, and Gingival Tissues of Chronic Periodontitis Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Sandy H. S. Hassan ◽  
Mahmoud I. El-Refai ◽  
Noha A. Ghallab ◽  
Rehab Fawzy Kasem ◽  
Olfat G. Shaker
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Prognostic Biomarker ◽  
Bone Destruction ◽  
Gingival Index ◽  
Pocket Depth ◽  
Clinical Attachment Level ◽  
Crevicular Fluid ◽  
Open Flap Debridement ◽  
Attachment Level

Objectives. This study was undertaken to investigate the OPG profiles in gingival crevicular fluid (GCF), saliva, and gingival tissues of chronic periodontitis (CP) patients in response to open flap debridement (OFD).Subjects and Methods. The study included 30 subjects divided into 2 groups: 20 CP patients and 10 periodontally healthy subjects. Plaque index, gingival index, pocket depth, and clinical attachment level measurements were recorded for all subjects. GCF, salivary, and gingival samples were collected from all 30 subjects at baseline and 3 and 6 month after OFD from the 20 CP patients. GCF and salivary OPG levels were assessed by ELISA assay, while OPG expression in gingival tissues was examined by immunohistochemistry.Results. GCF, salivary and gingival OPG profiles were significantly higher in control subjects compared to CP patients at baseline (P<0.001). Within CP group, OPG levels in GCF, saliva, and gingival samples showed a significant increase at 3 and 6 months after OFD (P<0.001) compared to baseline. Although OPG values increased significantly in gingival samples and insignificantly in saliva after 3 months compared to 6 months, yet GCF levels were significantly decreased.Conclusions. OPG might be considered as a diagnostic and prognostic biomarker of periodontal bone destruction. This trial is registered withNCT02160613.

scholarly journals Comparative evaluation of platelet-rich fibrin versus beta-tri-calcium phosphate in the treatment of Grade II mandibular furcation defects using cone-beam computed tomography

2016 ◽  
Vol 10 (04) ◽  
pp. 496-506 ◽  
Author(s):  
Zeba Rahman Siddiqui ◽  
Rajesh Jhingran ◽  
Vivek Kumar Bains ◽  
Ruchi Srivastava ◽  
Rohit Madan ◽  
...  
Keyword(s):  
Cone Beam ◽  
Clinical Parameters ◽  
Clinical Attachment Level ◽  
Platelet Rich Fibrin ◽  
Group I ◽  
Furcation Defects ◽  
Grade Ii ◽  
Group Ii ◽  
Attachment Level ◽  
Vertical Height

ABSTRACT Objective: The objective of the study was to evaluate clinically and radiographically the efficacy of platelet-rich fibrin (PRF) versus β-tri-calcium phosphate (β-TCP) in the treatment of Grade II mandibular furcation defects. Materials and Methods: Forty-five Grade II furcation defect in mandibular molars which were assigned to open flap debridement (OFD) with PRF Group I (n = 15), to OFD with β-TCP Group II (n = 15), and to OFD alone Group III (n = 15) were analyzed for clinical parameters (probing pocket depth [PPD], vertical clinical attachment level [VCAL], horizontal clinical attachment level [HCAL], gingival recession, relative vertical height of furcation [r-VHF], and relative horizontal depth of furcation [r-HDF]) and radiographical parameters (horizontal depth of furcation [H-DOF], vertical height of furcation [V-HOF]) using cone-beam computed tomography (CBCT) at 6 months interval. Results: For clinical parameters, reduction in PPD and gain in VCAL and HCAL were higher in Group II as compared to Group I. Change in r-VHF and r-HDF was greater in Group II as compared to Group I. Mean percentage clinical vertical defect fill was higher in Group II as compared to Group I (58.52% ± 11.68% vs. 53.24% ± 13.22%, respectively). On CBCT, mean change at 6 months for all parameters showed nonsignificant difference between the two experimental groups. Mean change in V-HOF was higher in Group I as compared to Group II, but mean change in H-DOF and furcation width was more in Group II as compared to Group I. Conclusion: For both experimental and control groups, there was statistically significant improvement at 6 months follow-up from baseline values.

scholarly journals Gingival Crevicular Fluid Levels of Prolidase and Alkaline Phosphatase in Periodontitis

2017 ◽  
Vol 21 (3) ◽  
pp. 158-161
Author(s):  
Berrak Guven ◽  
Cigdem Turer
Keyword(s):  
Alkaline Phosphatase ◽  
Gingival Crevicular Fluid ◽  
Clinical Parameters ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Healthy Control ◽  
Periodontal Destruction ◽  
Crevicular Fluid ◽  
Fluid Levels ◽  
Attachment Level

SummaryBackground/Aim: The purpose of this study was to investigate gingival crevicular fluid (GCF) alkaline phosphatase (ALP) and prolidase levels in subjects with different periodontal status. Material and Methods: Fifteen periodontitis, fifteen gingivitis and fifteen healthy subject were included. GCF samples were collected from participants. Probing depth, clinical attachment level, gingival index was recorded. ALP and prolidase levels were determined in GCF by spectrophotometrically. Results: Higher values of ALP were found in periodontitis compared with gingivitis and healthy control (p<0.001). The values of prolidase were lower in periodontitis than healthy control (p<0.05). A statistically significant positive correlation was found between clinical parameters and ALP levels (p<0.001). There is no significant correlation between clinical parameters and prolidase levels (p>0.05). Additionally, no significant correlation was detected between ALP and prolidase (r= -0.309, p>0.05). Conclusion: Our preliminary data suggest that low prolidase level in periodontitis was not associated with ALP and clinical parameters, which represent periodontal destruction and inflammation.

scholarly journals A 12-month randomized controlled trial evaluating erythritol air-polishing versus curette/ultrasonic debridement of mandibular furcations in supportive periodontal therapy

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ingvild M. Ulvik ◽  
Terje Sæthre ◽  
Dagmar F. Bunæs ◽  
Stein Atle Lie ◽  
Morten Enersen ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Controlled Trial ◽  
Clinical Attachment Level ◽  
Treatment Difference ◽  
Randomized Controlled ◽  
Air Polishing ◽  
Probing Depth ◽  
Furcation Defects ◽  
Supportive Periodontal Therapy ◽  
Attachment Level

Abstract Background Due to complex morphology and limited access, the cleaning of the furcation area is extremely challenging. Therefore, novel therapeutic approaches need to be tested to potentially overcome debridement limitations. The aim of the present prospective 12-month study was to compare clinical and microbiological effects following erythritol air-polishing versus conventional mechanical debridement of furcation defects in a cohort of periodontal maintenance patients. Methods Twenty patients with grade II mandibular molar furcation defects volunteered to enroll in this single-centre, examiner masked, randomized controlled trial. In a split-mouth study design, two furcation sites in each patient were randomly assigned to either receive subgingival debridement using erythritol air-polishing (test) or conventional ultrasonic/curette debridement (control) at baseline, and at 3, 6, 9 and 12 months. Probing depth, clinical attachment level and bleeding on probing were recorded at 3-month intervals. Subgingival microbiological samples obtained at baseline, 6 and 12 months were analyzed using checkerboard DNA–DNA hybridization. Discomfort from treatment was scored at 12 months using a visual analogue scale. The differences between treatments, and time-points, were tested using multilevel analysis (mixed effect models and robust variance estimates). Results A significant reduction in probing depth took place following both treatments (p < 0.001). Control sites experienced a significant mean gain in clinical attachment level of 0.5 mm (± 0.2) (p = 0.004), whereas a non-significant gain of 0.4 mm (± 0.3) was observed at test sites (p = 0.119). At 6 months, a significant between-treatment difference of 0.8 mm (± 0.4) was observed in favor of the control (p = 0.032). No significant between-treatment differences were observed in microbial load or composition. Notably, at 12 months patients experienced significantly less discomfort following air-polishing compared with control (p = 0.001). Conclusions The 12-month observations indicate that erythritol air-polishing and conventional mechanical debridement both support clinical improvements. A significant between-treatment difference in clinical attachment level was, however, detected in favour of control debridement at 6 months. In terms of patient comfort, erythritol air-polishing is superior. Trial Registration: The clinical trial was retrospectively registered in ClinicalTrial.gov with registration NCT04493398 (07/28/2020).

scholarly journals Evaluation of PRF Efficiency in the Treatment of Infrabony Defects

PRILOZI ◽  
2020 ◽  
Vol 41 (1) ◽  
pp. 79-86
Author(s):  
Jana Milutinovic ◽  
Mirjana Popovska ◽  
Biljana Rusevska ◽  
Milan Nacevski ◽  
Stefan Anastasovski ◽  
...  
Keyword(s):  
Test Group ◽  
Control Group ◽  
Clinical Parameters ◽  
Flap Surgery ◽  
Clinical Attachment Level ◽  
Positive Effects ◽  
Probing Depth ◽  
Infrabony Defects ◽  
Open Flap Debridement ◽  
Attachment Level

AbstractAim: The present study aimed to investigate the effectiveness of PRF in the treatment of infrabony defects in patients with chronic periodontitis by evaluating the clinical outcome through periodontal depth, clinical attachment level at the baseline, 6 and 9 months post operatively.Material and Methods: Sixty infrabony defects with probing depth ≥ 5 mm were treated. The inclusion criterion was the necessity for surgical bilateral maxillary treatment. By using split-mouth study design, each patient had one side treated with conventional flap surgery and the other side with conventional flap surgery and PRF. Clinical parameters, such as probing depth (PD) and clinical attachment lost (CAL), were recorded in both groups at baseline, 6 and 9 months post operatively.Results: Positive effects for all clinical and radiographic parameters were evident in the group with PRF. Mean PD reduction demonstrated statistically significant greater results in the test group (4.00±1.07 mm) compared to the control one (4.83±0.99 mm), p = 0.003 after 9 months postoperatively. After 9 months, there were better results in the test group compared to the control group for CAL (5.60±1.61 mm, 6.20±1.58 mm), but statistically not significant.Conclusion: Additional use of PRF in the conventional surgical treatment of infrabony defects demonstrated better parameters than the open flap debridement alone.

scholarly journals Non-Surgical Periodontal Therapy with Adjunctive Amoxicillin/Metronidazole or Metronidazole When No Aggregatibacter actinomycetemcomitans Is Detected—A Randomized Clinical Trial

Antibiotics ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 686
Author(s):  
Holger F. R. Jentsch ◽  
Martin Dietrich ◽  
Sigrun Eick
Keyword(s):  
Clinical Trial ◽  
Randomized Clinical Trial ◽  
Gingival Crevicular Fluid ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Periodontal Therapy ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Crevicular Fluid ◽  
Systemic Antibiotics ◽  
Attachment Level

Background: The aim was to compare two different systemic antibiotics regimens adjunctive to non-surgical periodontal therapy when Aggregatibacter actinomycetemcomitans was not detected in the subgingival biofilm. Methods: A total of 58 patients with periodontitis and with no A. actinomycetemcomitans in the subgingival biofilm were treated with full-mouth subgingival instrumentation and either metronidazole (MET; n = 29) or amoxicillin/metronidazole (AMX/MET; n = 29). Probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were recorded at baseline, as well as after three and six months. Subgingival biofilm and gingival crevicular fluid were collected and analyzed for major periodontopathogens and biomarkers. Results: PD, CAL and BOP improved at 3 and 6 months (each p < 0.001 vs. baseline) with no difference between the groups. Sites with initial PD ≥ 6 mm also improved in both groups after 3 and 6 months (p < 0.001) with a higher reduction of PD in the AMX/MET group (p < 0.05). T. forsythia was lower in the AMX/MET group after 3 months (p < 0.05). MMP-8 and IL-1β were without significant changes and differences between the groups. Conclusion: When A. actinomycetemcomitans was not detected in the subgingival biofilm, the adjunctive systemic use of amoxicillin/metronidazole results in better clinical and microbiological outcomes of non-surgical periodontal therapy when the application of systemic antibiotics is scheduled.

Role of Interleukin- 34 together with Receptor Activator of Nuclear Factor kB ligand and Osteoprotegrin levels in Periodontal Disease

2021 ◽  
Vol 15 (7) ◽  
pp. 1521-1522
Author(s):  
Noor Ul Amin ◽  
I. Saleem Qureshi ◽  
Sana Naeem ◽  
S. Hassan Jan ◽  
Kamal Khan ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Control Group ◽  
Gingival Index ◽  
Disease Group ◽  
Clinical Attachment Level ◽  
Group I ◽  
Group Ii ◽  
Crevicular Fluid ◽  
Attachment Level

Aim: To compare the levels and relative ratios of Interleukin-34, osteoprotegrin, and factor kB ligand in the crevicular fluid of individuals with periodontal disease. Study design & setting: Randomized Clinical Trial, Frontier Medical And Dental College, Abbotabad Methodology: We enrolled 15 healthy subjects (Group I), 15 patients with chronic periodontal disease (Group II) and 15 patients with aggressive periodontal disease (Group III). At baseline and six weeks after NSPT, crevicular fluid and clinical measures were made. The GCF IL-34, factor kB ligand, and osteoprotegrin levels were measured by using ELISA and their relative ratios were computed. Result: Group II and III had significantly higher crevicular fluid IL-34 and factor kB ligand levels than the Control group (I), although crevicular fluid with osteoprotegrin levels was significantly decreased (P<0.05). GCF, IL-34, and RANKL levels reduced in Groups II and III six weeks after NSPT, although OPG concentrations increased statistically (P<0.05). Significantly positive association was observed between IL-34 and factor kB ligand, clinical attachment level, and gingival index, but not with osteoprotegrin. Conclusion: Gingival crevicular fluid IL-34 levels were elevated in individuals with periodontal disease and lowered during non surgical intervention, and its levels correlated positively with the factor kB ligand and osteoprotegrin ratios, clinical attachment level, and gingival index. Keywords: Periodontitis, IL-34, osteoprotegrin

scholarly journals Inpatient Evaluation of Periodontal, Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth

2013 ◽  
Vol 14 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Güliz N Güncü ◽  
Gülüzar Büyüktepe ◽  
Sezen B AÕkin ◽  
Kamer Klllnç ◽  
Celal Tümer ◽  
...  
Keyword(s):  
Dental Implants ◽  
Gingival Crevicular Fluid ◽  
Gingival Index ◽  
Clinical Attachment Level ◽  
Inflammatory Parameters ◽  
Probing Depth ◽  
Natural Teeth ◽  
Fixed Prosthesis ◽  
Crevicular Fluid ◽  
Attachment Level

ABSTRACT Aim The use of endosseous dental implants (DI) has become a successful treatment alternative. However, providing periimplant tissue health and achieving a natural esthetic look are important topics in this treatment. The aim of the present study was to evaluate periodontal and esthetic parameters around DI and natural teeth (NT) and also to analyze myeloperoxidase (MPO) levels in gingival crevicular fluid (GCF) and peri-implant sulcus fluid (PISF). Materials and methods Twenty DI supported fixed prosthesis and contralateral 20 NT were enrolled to the present study. Clinical periodontal parameters (probing depth, clinical attachment level, gingival bleeding time index and gingival index) were recorded and GCF/PISF samples were obtained from mesial (mesiobuccal and mesiolingual) and distal (distobuccal and distolingual) sites of DI and NT. MPO levels were spectrophotometrically determined. Additionally clinical photographs were obtained and esthetical evaluations were performed by using Jemt papilla index. The parameters belong to DI and NT were compared and correlations were evaluated using statistical analysis. Results A total of 40 samples were evaluated. No statistically significant differences were detected between groups in all periodontal parameters and MPO levels from mesial and distal sites. Jemt papilla index scores were slightly higher in NT however, this difference was not statistically significant (p > 0.05). Total PES score were similiar in DI and NT groups. Significant correlations were detected between MPO and gingival index values as expected. Clinical significance Dental implants are satisfactory treatments, they provide patient esthetic natural looking, phonetic and masticatory functions. How to cite this article Güncü GN, Büyüktepe G, AÕkin SB, KlllnH K, Tümer C, Tözüm TF. Inpatient Evaluation of Periodontal Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth. J Contemp Dent Pract 2013;14(1):90-95.

scholarly journals LL-37 and hBD-2 in the gingival crevicular fluid of smokers and nonsmokers with periodontitis

2021 ◽  
Vol 50 ◽  
Author(s):  
Alessandra Barreto LOPES ◽  
Natália Helena COLOMBO ◽  
Naida Zanini ASSEM ◽  
Marta Aparecida Alberton NUERNBERG ◽  
Valdir Gouveia GARCIA ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Epidemiological Studies ◽  
Clinical Parameters ◽  
Clinical Attachment Loss ◽  
Probing Depth ◽  
Significant Difference ◽  
Level Of Significance ◽  
Crevicular Fluid ◽  
Attachment Level

Abstract Introduction The association between smoking and periodontal diseases has been described in clinical and epidemiological studies. Objective The aim of this study was to compare the LL-37 and human β-defensin-2 (hBD-2) levels in crevicular fluid of patients with generalized periodontitis in smokers (S) and nonsmokers (NS). Material and method A total of 35 patients with generalized periodontitis stages III and IV, 15 NS (11 female, 4 male) and 20 S (7 female and 13 male), were included in the study. The evaluated clinical parameters were bleeding on probing (BOP), probing depth (PD) and clinical attachment level (CAL). Enzyme-linked immunosorbent assays were performed to quantify the LL-37 and hBD-2 levels in the gingival crevicular fluid of these patients. The results were analyzed statistically with the level of significance set at 5%. Result In relation to periodontal clinical parameters, no statistically significant difference was observed for BOP and PD between groups S and NS. There was a higher CAL in the S group than in the NS group (p = 0.0095). There was no statistically significant difference between the levels of LL-37 and hBD-2 when comparing groups S and NS (p>0.05). Conclusion It was concluded that smokers have a higher clinical attachment loss than nonsmokers, but that smoking did not influence the levels of LL-37 and hBD-2 in the gingival crevicular fluid in periodontitis.

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