scholarly journals A simple method for the spectrophotometric determination of cephalosporins in pharmaceuticals using variamine blue

2018 ◽  
Vol 33 (2) ◽  
pp. 41
Author(s):  
Chand Pasha ◽  
Badiadka Narayana
Keyword(s):  
Sodium Hydroxide ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Acidic Medium ◽  
Simple Method ◽  
Lactam Ring ◽  
Ph Range ◽  
Variamine Blue ◽  
Hydrolysis Of

A simple spectrophotometric method for the determination of cefotaxime, ceftriaxone, cefadroxil and cephalexin with variamine blue is presented. The determination is based on the hydrolysis of β-lactam ring of cephalosporins with sodium hydroxide which subsequently reacts with iodate to liberate iodine in acidic medium. The liberated iodine oxidizes variamine blue to violet colored species of maximum absorption at 556 nm. The absorbance is measured within the pH range of 4.0-4.2. Beer’s law is obeyed in the range of 0.5-5.8 μg mL–1, 0.2-7.0 μg mL–1, 0.2-5.0 μg mL–1 and 0.5-8.5 μg mL–1 for cefotaxime, ceftriaxone, cefadroxil and cephalexin respectively. The analytical parameters were optimized and the method is successfully applied for the determination of cefotaxime, ceftriaxone, cefadroxil and cephalexin in pharmaceuticals.

Collaborative Study of the Spectrophotometric Determination of Procainamide Hydrochloride

1976 ◽  
Vol 59 (4) ◽  
pp. 807-810
Author(s):  
Jeffrey C Hamm
Keyword(s):  
Sodium Hydroxide ◽  
Acid Medium ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Collaborative Study ◽  
Dosage Forms ◽  
Standard Deviations

Abstract The USP analysis for procainamide HCl is titrimetric and relatively nonspecific, capsule and tablet dyes may interfere, and the method is not applicable to coated tablets. In the spectrophotofluorometric method the sample deteriorates when exposed to a xenon source. In the ultraviolet spectrophotometric method reported here, the sample is dispersed in acid medium, possible interferences are extracted in chloroform, base is added, procainamide is extracted in chloroform, the residue is dissolved in sodium hydroxide, and the compound is measured by absorption at 272 nm and comparison with a standard. Recoveries of standards added to capsule, tablet, and injection composites ranged from 99.3 to 102%. Twelve collaborators reported duplicate assay results for all 3 dosage forms with per cent standard deviations for 5 samples ranging from 1.01 to 1.27%. The method has been adopted as official first action.

A new optical sensor for spectrophotometric determination of uranium (VI) and thorium (IV) in acidic medium

2017 ◽  
Vol 105 (12) ◽  
Author(s):  
O. A. Elhefnawy
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Optical Sensor ◽  
Acidic Medium ◽  
Complexation Reaction

AbstractA spectrophotometric method was developed for U(VI) and Th(IV) determination in acidic medium by using proposed optical sensor. This sensor is base on the complexation reaction of the ionophore midodrine hydrochloride (

scholarly journals Spectrophotometric Determination of Paracetamol in bulk and Pharmaceutical Preparations

2015 ◽  
Vol 12 (2) ◽  
pp. 317-323
Author(s):  
Baghdad Science Journal
Keyword(s):  
Sodium Hydroxide ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Reaction Conditions ◽  
Accuracy And Precision ◽  
Beer's Law ◽  
Beer’S Law

A simple, and rapid spectrophotometric method for the estimation of paracetamol has been developed. The methods is based on diazotisation of 2,4-dichloroaniline followed by a coupling reaction with paracetamol in sodium hydroxide medium. All variables affecting the reaction conditions were carefully studied. Beer's law is obeyed in the concentration range of 4-350 ?gml?1 at 490 nm .The method is successfully employed for the determination of paracetamol in pharmaceutical preparations. No interferes observed in the proposed method. Analytical parameters such as accuracy and precision have been established for the method and evaluated statistically to assess the application of the method.

scholarly journals Spectrophotometric determination of pyridoxal and pyridoxal 5′-phosphate with 3-methyl-2-benzothiazolone hydrazone hydrochloride, and their selective assay

1969 ◽  
Vol 114 (3) ◽  
pp. 629-633 ◽  
Author(s):  
Kenji Soda ◽  
Takamitsu Yorifuji ◽  
Haruo Misono ◽  
Mitsuaki Moriguchi
Keyword(s):  
Absorption Spectra ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
The Other ◽  
Selective Determination ◽  
Characteristic Absorption ◽  
Ph Values ◽  
Alkaline Conditions ◽  
Ph Range

A spectrophotometric method with 3-methyl-2-benzothiazolone hydrazone hydrochloride was developed for the determination of pyridoxal and pyridoxal 5′-phosphate, and for the selective determination of each in the presence of the other. Pyridoxal and pyridoxal 5′-phosphate react with the reagent to yield the azine derivatives, which give characteristic absorption spectra. The highest extinction values are obtained when pyridoxal and pyridoxal 5′-phosphate are incubated at pH values of about 3·4 and 8·0 respectively; their maxima are at 430nm. (∈ 2·74×104) and 380nm. (∈ 2·24×104) respectively. The azine of pyridoxal is only slightly soluble under the neutral and alkaline conditions, whereas that of pyridoxal 5′-phosphate is substantially insoluble in the acid pH range. This difference in solubility of the azines made possible the selective determination of pyridoxal and pyridoxal 5′-phosphate. α-Oxoglutarate and pyruvate are among the substances shown not to interfere with the assay of pyridoxal; their derivatives absorb appreciably only at wavelengths below 420nm. For the assay of pyridoxal 5′-phosphate in the presence of these compounds measurement at 390nm. is necessary.

scholarly journals A Simple, Sensitive Spectrophotometric Determination of Mosapride in Pharmaceutical Preparations Using Novel Reagent

2004 ◽  
Vol 1 (5) ◽  
pp. 267-271 ◽  
Author(s):  
G. Krishnamurthy Naidu ◽  
K. Suvardhan ◽  
M. V. Jyothi ◽  
B. S. Sastry ◽  
P. Chiranjeevi
Keyword(s):  
Optimum Condition ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Azo Dye ◽  
Acidic Medium ◽  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Pharmaceutical Formulation ◽  
Sensitive Spectrophotometric Method

A rapid, selective and sensitive spectrophotometric method for the determination of mosarpide in either pure form or in its formulations in pharmaceutical preparations is described. The method is based on the diazotization coupling reaction of mosapride with 1-naphthol in acidic medium to give red azo dye product with theλmaxof 500 nm and is stable for more than 12 hours at optimum condition, Beer's law is obeyed at concentration of 0.2 to 12 μg mL-1at the wavelength of maximum absorbance. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method. The developed method is highly reproducible and applied to wide variety of pharmaceutical formulation and the results were compared favourably with the reported method.

Benzyl ethers of methyl α-D-glucopyranoside

1980 ◽  
Vol 45 (7) ◽  
pp. 1959-1963 ◽  
Author(s):  
Dušan Joniak ◽  
Božena Košíková ◽  
Ludmila Kosáková
Keyword(s):  
Kinetic Study ◽  
Spectrophotometric Determination ◽  
Reductive Cleavage ◽  
Ether Bond ◽  
Benzyl Ethers ◽  
Acid Catalyzed ◽  
Model Substances ◽  
Hydrolysis Of

Methyl 4-O-(3-methoxy-4-hydroxybenzyl) and methyl 4-O-(3,5-dimethoxy-4-hydroxybenzyl)-α-D-glucopyranoside and their 6-O-isomers were prepared as model substances for the ether lignin-saccharide bond by reductive cleavage of corresponding 4,6-O-benzylidene derivatives. Kinetic study of acid-catalyzed hydrolysis of the compounds prepared was carried out by spectrophotometric determination of the benzyl alcoholic groups set free, after their reaction with quinonemonochloroimide, and it showed the low stability of the p-hydroxybenzyl ether bond.

Determination of Unconjugated and Total N-Acetyl-p-aminophenol (NAPA) in Urine and Serum

1967 ◽  
Vol 13 (3) ◽  
pp. 215-219 ◽  
Author(s):  
Karel P M Heirwegh ◽  
Johan Fevery
Keyword(s):  
Liver Disease ◽  
Acid Hydrolysis ◽  
Azo Dye ◽  
Acidic Medium ◽  
Diazonium Salt ◽  
Accurate Method ◽  
Total N ◽  
Hydrolysis Of ◽  
Urine And Serum

Abstract A sensitive and accurate method is described for the determination of N-acetyl-p-aminophenol (NAPA) and its metabolites in urine and serum. In strongly acidic medium, p-aminophenol (PAP) resulting from differential extraction and acid hydrolysis of total NAPA and unconjugated NAPA, is diazotized and the diazonium salt coupled with N-(1-naphthyl)ethylenediamine (NED) in the presence of ethanol. The blue azo dye formed is determined spectrophotometrically. Application to liver disease is briefly reported.

DC Polarographic and Spectrophotometric Determination of Ampicillin Capsules

1980 ◽  
Vol 63 (5) ◽  
pp. 1049-1051
Author(s):  
Juan A Squella ◽  
Luis J Nunez-Vergara ◽  
Maximo Aros
Keyword(s):  
Absorption Band ◽  
Spectrophotometric Determination ◽  
Degradation Product ◽  
Uv Absorption ◽  
Acidic Hydrolysis ◽  
Average Recovery ◽  
Polarographic Wave ◽  
Spectrophotometric Methods ◽  
Hydrolysis Of

Abstract Polarographic and spectrophotometric methods are proposed for the determination of ampicillin in capsules. Acidic hydrolysis of ampicillin with 1% HCHO in 0.3N HCl yields a degradation product identified as 2-hydroxy-3-phenyl-6-methylpyrazine. This compound has a well defined UV absorption band at 380 nm and a polarographic wave at –0.55 V vs SCE, which can be used for analytical purposes. Individual capsule assays, composite assays, and recovery studies are described. The average recovery values and standard deviations (SD) for UV and polarographic determinations were 99.20% (SD 0.95) and 100.85% (SD 1.09), respectively

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