scholarly journals Mengungkap Senyawa pada Nata De Coco sebagai Pangan Fungsional

2019 ◽  
Vol 3 (1) ◽  
pp. 42
Author(s):  
Choiroel Anam

 Nata adalah selulosa ekstraseluler yang dihasilkan dari aktiivitas bakteri Acetobacter xylinum dalam proses fermentasi, dan  merupakan salah satu makanan kesehatan yang kaya akan serat. Nata yang paling umum ditemukan adalah nata de coco dengan media fermentasi air kelapa. Faktor-faktor dominan dalam pembuatan nata adalah ketersediaan nutrisi (karbon dan nitrogen), derajat keasaman dan media fermentasi yang digunakan. Penentuan kualitas terbaik media air kelapa yang digunakan, penentuan konsentrasi nitrogen dan sukrosa yang ditambahkan serta kondisi pH optimum  akan menghasilkan nata yang maksimal.Penelitian ini bertujuan untuk mencari kualitas terbaik pada fermentasi dalam proses pembuatan nata de coco, dan mengetahui senyawa fungsional yang terdapat pada nata de coco sebagai pangan fungsional. Hasil pengujian analisa sampel nata de coco menggunakan Gas chromatography–mass spectrometry (GC-MS) menunjukkan senyawa yang sangat bermaaf bagi kesehatan tubuh manusia. Sepuluh besar senyawa fungsional yag terdapat pada nata de coco yaitu:  Benzeneacetic Acid sebagai anti fungal dan scavenger. Hexadecanoid Acid memiliki efek anti-inflamasi, anti bakteri dan anti fungi. 22-Hydroxyhopane, Tetradecanoic Acid yang mempunyai aktivitas antimikroba dan antifungal. 9-Octadecanoid Acid, ρ-Cresol  memiliki aktivitas antioksidan. 9-Octadecenamide berfungsi untuk mencegah Alzheimer, menurunkan kolesterol dan menurunkan tekanan darah, Senyawa fungsional yang lain diantaranya (Z), Phenol, 4-(2-aminoethyl), Pentadecanoic Acid, 1-Heptadecanecarboxylic acid. Komposisi kimia nata  de coco dihasilkan kadar air 95%.  abu 0,35% dan protein 0,45%. Penelitian menunjukkan peningkatkan produksi nata dengan kualitas  yang baik sesuai persyaratan standar yang telah ditetapkan dan mempunyai senyawa senyawa  yang bermanfaat sebagai pangan fungsional yang bermanfaat bagi kesehatan.

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Mingjie Deng ◽  
Meiling Zhang ◽  
Fa Sun ◽  
Jianshe Ma ◽  
Lufeng Hu ◽  
...  

Gas chromatography-mass spectrometry (GS-MS) in combination with multivariate statistical analysis was applied to explore the metabolic variability in urine of chronically hydrogen sulfide- (H2S-) poisoned rats relative to control ones. The changes in endogenous metabolites were studied by partial least squares-discriminate analysis (PLS-DA) and independent-samplest-test. The metabolic patterns of H2S-poisoned group are separated from the control, suggesting that the metabolic profiles of H2S-poisoned rats were markedly different from the controls. Moreover, compared to the control group, the level of alanine, d-ribose, tetradecanoic acid, L-aspartic acid, pentanedioic acid, cholesterol, acetate, and oleic acid in rat urine of the poisoning group decreased, while the level of glycine, d-mannose, arabinofuranose, and propanoic acid increased. These metabolites are related to amino acid metabolism as well as energy and lipid metabolismin vivo. Studying metabolomics using GC-MS allows for a comprehensive overview of the metabolism of the living body. This technique can be employed to decipher the mechanism of chronic H2S poisoning, thus promoting the use of metabolomics in clinical toxicology.


2021 ◽  
Vol 948 (1) ◽  
pp. 012059
Author(s):  
S Zulfina ◽  
M A N Fathoni ◽  
R Poerwanto ◽  
D D Matra

Abstract Indonesia is a high biodiversity country for underutilized fruits. Indonesian underutilized fruits contain several beneficial functional foods that are very useful for human health. This study aimed to analyze the secondary metabolites found in Kasturi mango (Mangifera casturi). Fruit sampling was carried out in Hulu Sungai Selatan and Banjar, South Kalimantan. Five types of Kasturi mango were used, namely Kasturi, Pinari, Palipisan, Cuban, and Rawa-rawa, which included aril and rind. The results of this research showed that there are specific compounds in each type. Analysis of secondary metabolites using gas chromatography-mass spectrometry (GC-MS) in Kasturi aril showed that specific compounds were obtained in the Kasturi type, such as 13-tetradecenal ethyl elaidate, spinacene, and (23S)-ethylcholest-5-en-3.beta.-ol. The Rawa-rawa type contained specific compounds such as tetradecanoic acid and cis-vaccenic acid. Analysis of secondary metabolites with GC-MS in Kasturi rind showed that the Kasturi type contained specific compounds, such as distearil phosphite and 13-octadecenoic acid. The Rawa-rawa type contained specific compounds such as docosane and triacontyl acetate. Based on, characterization of secondary metabolites using GC-MS, Kasturi mango showed the high variation of secondary metabolites among their types.


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