scholarly journals Determination of free fatty acids in brown algae of the White Sea by MALDI-TOF mass spectrometry using Langmuir technology

Author(s):  
A.S. Gladchuk ◽  
◽  
P.S. Dubakova ◽  
M.L. Alexandrova ◽  
K.A. Krasnov ◽  
...  
2002 ◽  
Vol 215 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Thomas Kislinger ◽  
Andreas Humeny ◽  
Silke Seeber ◽  
Cord-Michael Becker ◽  
Monika Pischetsrieder

Metabolites ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 40 ◽  
Author(s):  
Maroula G. Kokotou ◽  
Christiana Mantzourani ◽  
Rodalia Babaiti ◽  
George Kokotos

The lipidome of royal jelly (RJ) consists of medium-chained (8–12 carbon atoms) free fatty acids. We present herein a liquid chromatography-high resolution mass spectrometry (HRMS) method that permits the determination of RJ fatty acids and at the same time the detection of suspect fatty acids. The method allows for the direct quantification of seven free fatty acids of RJ, avoiding any derivatization step. It was validated and applied in seven RJ samples, where the major RJ fatty acid trans-10-hydroxy-2-decenoic acid (10-HDA) was found to vary from 0.771 ± 0.08 to 0.928 ± 0.04 g/100 g fresh RJ. Four additional suspect fatty acids were simultaneously detected taking advantage of the HRMS detection.


Bacteriology ◽  
2020 ◽  
Vol 5 (3) ◽  
pp. 8-13
Author(s):  
A.A. Samoilova ◽  
◽  
I.V. Likhachev ◽  
E.V. Zueva ◽  
E.V. Rogacheva ◽  
...  

Actual microbiological diagnostics of infections caused by Klebsiella spp. should include isolation of the strain, its identification and fastest possible determination of the pathogen susceptibility to antimicrobial agents. We evaluated the prospects of using MALDI-TOF mass spectrometry to determine the susceptibility of Klebsiella spp. strains to antimicrobial agents. According to the results of mass spectrometry Klebsiella spp. strains analysis, we carried out cluster analysis by the UPGMA method based on mass spectra and data on the susceptibility of the studied strains to antimicrobial agents, and then studied the obtained dendrograms. We identified the areas with the highest probability of the location of antibiotic resistance markers by comparing the mass spectra of susceptible and resistant microorganisms at different concentrations of antimicrobial agents. Thus, using MALDI-TOF mass spectrometry, a new direction in assessing the susceptibility of Klebsiella spp. to antimicrobial agents can be formed. Key words: antibiotic resistance, Klebsiella spp., MALDI-TOF mass spectrometry


2012 ◽  
Vol 358 ◽  
pp. 78-81 ◽  
Author(s):  
Stanislav D. Anastyuk ◽  
Natalia M. Shevchenko ◽  
Pavel S. Dmitrenok ◽  
Tatyana N. Zvyagintseva

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