Rapidity and multiplicity of synthesis and expression of immunoglobulin isotypes by B lymphocytes in the small intestine

10.2741/1312 ◽  
2004 ◽  
Vol 9 (1-3) ◽  
pp. 1642 ◽  
Author(s):  
Stephen Douglas Wagner
Keyword(s):  
Small Intestine ◽  
B Lymphocytes ◽  
Immunoglobulin Isotypes

Polyclonal activation of B lymphocytes during experimental infection with Schistosoma mansoni

Parasitology ◽  
1990 ◽  
Vol 100 (1) ◽  
pp. 83-91 ◽  
Author(s):  
L. M. Lopes ◽  
M. A. C. Pereira ◽  
S. E. Gerken ◽  
N. Vaz
Keyword(s):  
Schistosoma Mansoni ◽  
B Lymphocytes ◽  
Experimental Infection ◽  
Adoptive Transfer ◽  
Cell Activation ◽  
Protein A ◽  
B Cell Activation ◽  
Spleen Cells ◽  
Polyclonal Activation ◽  
Immunoglobulin Isotypes

SummaryA significant polyclonal activation of B lymphocytes was observed during experimental infection of C57BL/10J mice with Schistosoma mansoni. The isotypic pattern of this expansion, assessed by the Protein-A plaque-forming cell method, was compared with and found to differ from those occurring after infection by Trypanosoma cruzi or injection of bacterial LPS. In the infection of S. mansoni an early expansion of most immunoglobulin isotypes occurs together with a late, sustained expansion of IgG1-secreting cells. High levels of polyclonal B cell activation were observed after adoptive transfer of spleen cells from infected mice to isogenic recipients pre-treated with hydroxyurea.

Immune disorders of the gastrointestinal tract

2010 ◽  
pp. 2244-2256
Author(s):  
D.J. Unsworth
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
B Lymphocytes ◽  
Lamina Propria ◽  
Thoracic Duct ◽  
Lymphoid Tissue ◽  
Plasma Cells ◽  
Immunoglobulin A ◽  
Systemic Circulation ◽  
Lymphoid Follicles

The gastrointestinal tract is protected by gut-associated lymphoid tissue that provides an environment where interaction occurs between luminal antigen and specially adapted immune tissue in Peyer’s patches (small intestine only) or lymphoid follicles. T and B lymphocytes primed in the gut migrate into the systemic circulation via the thoracic duct but home preferentially to the lamina propria of the intestine. Plasma cells of the lamina propria secrete immunoglobulin A as a dimer linked by a joining peptide....

Immunophenotype of the inflammatory response in the central and enteric nervous systems of cockatiels (Nymphicus hollandicus) experimentally infected with parrot bornavirus 2

2022 ◽  
pp. 030098582110691
Author(s):  
Jeann Leal de Araújo ◽  
Raquel R. Rech ◽  
Aline Rodrigues-Hoffmann ◽  
Paula R. Giaretta ◽  
Cinthya Cirqueira ◽  
...  
Keyword(s):  
Small Intestine ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Inflammatory Infiltrate ◽  
Homogeneous Distribution ◽  
Nymphicus Hollandicus ◽  
Inflammatory Infiltrates ◽  
Cellular Immune ◽  
Proventricular Dilatation Disease ◽  
The Brain

Proventricular dilatation disease is a lethal disease of psittacine birds. In this study, we characterized the local cellular immune response in the brain, proventriculus, and small intestine of 27 cockatiels ( Nymphicus hollandicus) experimentally infected with parrot bornavirus 2 (PaBV-2). Perivascular cuffs in the brain were composed of CD3+ T-lymphocytes and Iba1+ macrophages/microglia in most cockatiels (n = 26). In the ganglia of the proventriculus, CD3+ T-lymphocytes (n = 17) and Iba1+ macrophages (n = 13) prevailed. The ganglia of the small intestine had a more homogeneous distribution of these leukocytes, including PAX5+ B-lymphocytes (n = 9), CD3+ T-lymphocytes (n = 8), and Iba1+ macrophages (n = 8). Our results indicate that perivascular cuffs in the brain and the inflammatory infiltrate in the proventriculus of PaBV-2-infected cockatiels is predominately composed of T-lymphocytes, while the inflammatory infiltrates in the ganglia of the small intestine are characterized by a mixed infiltrate composed of T-lymphocytes, B-lymphocytes, and macrophages.

scholarly journals Human B lymphocyte subsets. I. IgG-bearing B cell response to pokeweed mitogen.

1981 ◽  
Vol 153 (2) ◽  
pp. 339-351 ◽  
Author(s):  
K A Ault ◽  
M Towle
Keyword(s):  
B Cells ◽  
B Lymphocytes ◽  
B Lymphocyte ◽  
Pokeweed Mitogen ◽  
Small Subset ◽  
Strong Response ◽  
Human Spleen ◽  
Immunoglobulin Isotypes ◽  
Accessory Cells ◽  
Two Populations

The subset of B lymphocytes having IgG on their surfaces was purified from human spleen and blood using a fluorescence-activated cell sorter (FACS). This subset constituted about 15% of B lymphocytes. The remaining non-IgG-bearing B cells were also obtained for study. These two populations were examined for (a) their expression of other surface immunoglobulin isotypes, (b) their ability to give rise to IgG- and IgM-secreting (plaque-forming) cells in a pokeweed mitogen (PWM)-driven culture system, and (c) their ability to proliferate in response to PWM stimulation. The results of these studies indicate that most IgG-bearing B cells also express surface IgM and IgD. Less than 15% had only IgG. The IgG-positive cell gave rise to both IgG and IgM plaque-forming cells when driven by PWM, and in fact were responsible for most of the total plaque response in both the IgG and IgM classes. The non-IgG-bearing B cells were depleted of both IgG and IgM responsiveness. The failure of the non-IgG-bearing B cells to give a strong response to PWM did not appear to be due to either depletion of accessory cells or to any suppressive influence. Finally, proliferation studies indicated that both the IgG-bearing and the non-IgG-bearing cells proliferated in the presence of PWM with a somewhat stronger proliferative response in the IgG-bearing cells. These results demonstrate that the IgG-bearing cell is not irreversibly committed to IgG production but can also give rise to IgM-secreting cells, and that human PWM-driven immunoglobulin secretory responses are predominantly due to a numerically small subset of B cells.

scholarly journals Cell surface immunoglobulin. XVIII. Functional differences of B lymphocytes bearing different surface immunoglobulin isotypes.

1976 ◽  
Vol 143 (5) ◽  
pp. 1055-1065 ◽  
Author(s):  
E S Vitetta ◽  
J Forman ◽  
J R Kettman
Keyword(s):  
Cell Surface ◽  
B Cell ◽  
B Lymphocytes ◽  
Surface Immunoglobulin ◽  
Functional Differences ◽  
Immunoglobulin Isotypes ◽  
Cell Mitogen

Three populations of murine splenic B lymphocytes have been characterized previously (6, 7, 9) as those bearing only IgM, those bearing only IgD, and a population bearing both isotopes. These studies were designed to test the response of the IgM+ cells (IgM-only or IgM plus IgD) vs. the IgD-only cells to the B-cell mitogen, lipopolysaccharide. Results that after 1-4 days of culture, in the presence of mitogen, the IgM+ cells enlarge and elaborate an IgM polyclonal response. The IgD-only cells, in contrast, do not exhibit an IgM polyclonal response, but instead undergo blastogenesis and proliferation.

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