Coexpression of Multiple Immunoglobulin Isotypes on Human B-Lymphocytes

SummaryA significant polyclonal activation of B lymphocytes was observed during experimental infection of C57BL/10J mice with Schistosoma mansoni. The isotypic pattern of this expansion, assessed by the Protein-A plaque-forming cell method, was compared with and found to differ from those occurring after infection by Trypanosoma cruzi or injection of bacterial LPS. In the infection of S. mansoni an early expansion of most immunoglobulin isotypes occurs together with a late, sustained expansion of IgG1-secreting cells. High levels of polyclonal B cell activation were observed after adoptive transfer of spleen cells from infected mice to isogenic recipients pre-treated with hydroxyurea.

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Identification of immunoglobulin isotypes on human B lymphocytes

Clinical Immunology Newsletter ◽

10.1016/s0197-1859(82)80037-x ◽

1982 ◽

Vol 3 (21) ◽

pp. 147-153 ◽

Cited By ~ 2

Author(s):

Clement C.S. Hsu

Keyword(s):

B Lymphocytes ◽

Immunoglobulin Isotypes

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Abnormal expression of surface immunoglobulin isotypes on antigen-binding B lymphocytes from mice tolerant to trinitrophenyl determinants

Cellular Immunology ◽

10.1016/0008-8749(87)90248-6 ◽

1987 ◽

Vol 107 (2) ◽

pp. 408-416

Author(s):

Richard D. Brasington ◽

Mary E. Morton ◽

Cynthia E. Baumgartner ◽

Robert F. Ashman

Keyword(s):

B Lymphocytes ◽

Antigen Binding ◽

Surface Immunoglobulin ◽

Abnormal Expression ◽

Immunoglobulin Isotypes

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Human B lymphocyte subsets. I. IgG-bearing B cell response to pokeweed mitogen.

Journal of Experimental Medicine ◽

10.1084/jem.153.2.339 ◽

1981 ◽

Vol 153 (2) ◽

pp. 339-351 ◽

Cited By ~ 35

Author(s):

K A Ault ◽

M Towle

Keyword(s):

B Cells ◽

B Lymphocytes ◽

B Lymphocyte ◽

Pokeweed Mitogen ◽

Small Subset ◽

Strong Response ◽

Human Spleen ◽

Immunoglobulin Isotypes ◽

Accessory Cells ◽

Two Populations

The subset of B lymphocytes having IgG on their surfaces was purified from human spleen and blood using a fluorescence-activated cell sorter (FACS). This subset constituted about 15% of B lymphocytes. The remaining non-IgG-bearing B cells were also obtained for study. These two populations were examined for (a) their expression of other surface immunoglobulin isotypes, (b) their ability to give rise to IgG- and IgM-secreting (plaque-forming) cells in a pokeweed mitogen (PWM)-driven culture system, and (c) their ability to proliferate in response to PWM stimulation. The results of these studies indicate that most IgG-bearing B cells also express surface IgM and IgD. Less than 15% had only IgG. The IgG-positive cell gave rise to both IgG and IgM plaque-forming cells when driven by PWM, and in fact were responsible for most of the total plaque response in both the IgG and IgM classes. The non-IgG-bearing B cells were depleted of both IgG and IgM responsiveness. The failure of the non-IgG-bearing B cells to give a strong response to PWM did not appear to be due to either depletion of accessory cells or to any suppressive influence. Finally, proliferation studies indicated that both the IgG-bearing and the non-IgG-bearing cells proliferated in the presence of PWM with a somewhat stronger proliferative response in the IgG-bearing cells. These results demonstrate that the IgG-bearing cell is not irreversibly committed to IgG production but can also give rise to IgM-secreting cells, and that human PWM-driven immunoglobulin secretory responses are predominantly due to a numerically small subset of B cells.

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Cell surface immunoglobulin. XVIII. Functional differences of B lymphocytes bearing different surface immunoglobulin isotypes.

Journal of Experimental Medicine ◽

10.1084/jem.143.5.1055 ◽

1976 ◽

Vol 143 (5) ◽

pp. 1055-1065 ◽

Cited By ~ 23

Author(s):

E S Vitetta ◽

J Forman ◽

J R Kettman

Keyword(s):

Cell Surface ◽

B Cell ◽

B Lymphocytes ◽

Surface Immunoglobulin ◽

Functional Differences ◽

Immunoglobulin Isotypes ◽

Cell Mitogen

Three populations of murine splenic B lymphocytes have been characterized previously (6, 7, 9) as those bearing only IgM, those bearing only IgD, and a population bearing both isotopes. These studies were designed to test the response of the IgM+ cells (IgM-only or IgM plus IgD) vs. the IgD-only cells to the B-cell mitogen, lipopolysaccharide. Results that after 1-4 days of culture, in the presence of mitogen, the IgM+ cells enlarge and elaborate an IgM polyclonal response. The IgD-only cells, in contrast, do not exhibit an IgM polyclonal response, but instead undergo blastogenesis and proliferation.

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Rapidity and multiplicity of synthesis and expression of immunoglobulin isotypes by B lymphocytes in the small intestine

Frontiers in Bioscience ◽

10.2741/1312 ◽

2004 ◽

Vol 9 (1-3) ◽

pp. 1642 ◽

Cited By ~ 2

Author(s):

Stephen Douglas Wagner

Keyword(s):

Small Intestine ◽

B Lymphocytes ◽

Immunoglobulin Isotypes

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A dichotomy between the expression of IgD on B cells and its requirement for triggering such cells with two T-independent antigens

Journal of Experimental Medicine ◽

10.1084/jem.149.4.987 ◽

1979 ◽

Vol 149 (4) ◽

pp. 987-992 ◽

Cited By ~ 12

Author(s):

LB Buck ◽

D Yuan ◽

ES Vitetta

Keyword(s):

B Cells ◽

B Lymphocytes ◽

Personal Communication ◽

Small Population ◽

Sheep Erythrocytes ◽

Immunoglobulin Isotypes ◽

Low Levels

The majority of adult B lymphocytes in the mouse bear two immunoglobulin isotypes, IgM and IgD (μ(+)δ(+) cells) (1). A small population of IgM-bearing cells lacks, or expresses very low levels of IgD (μ- predominant [μp] cells) (1). These cells are believed to constitute a less mature subset of B cells analogous to neonatal B cells (2). Based on the time during ontogeny when responses to T-independent (TI) and T-dependent (TD) antigens appear (3, 4) and the ability to block in vitro responses with anti- μ or anti-δ (5, 6, D. Mosier, personal communication), it has been suggested that the precursors of two TI-1 responses, trinitrophenyl (TNP)- Brucella (TNP-BA) and TNP-lipopolysaccharide (TNP-LPS) are μp cells (5, 6), whereas the precursor for a TD response, TNP-sheep erythrocytes (TNP-SRBC), bears both IgM and IgD (6). However, the possibility cannot be excluded that IgD is present on some or all of the TI precursors, but that it is not obligatory for triggering. In the present experiments we have examined the phenotypes of TI and TD precursors by treating cells with C' and either anti-μ or anti-δ before stimulation with antigen. Our results suggest that the majority of B cells that respond to TNP-BA, TNP-LPS, and TNP-SRBC bear IgD, even though in the case of the two TI antigens, IgD is not required for triggering.

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Altered Fine Structure of B Lymphocytes Correlated with Defective Terminal Differentiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010007237x ◽

1973 ◽

Vol 31 ◽

pp. 386-387

Author(s):

Dale E. Bockman ◽

L. Y. Frank Wu ◽

Alexander R. Lawton ◽

Max D. Cooper

Keyword(s):

Immune Deficiency ◽

B Lymphocytes ◽

Plasma Cells ◽

Present Report ◽

Specific Antigen ◽

Terminal Differentiation ◽

Second Stage ◽

Two Stages ◽

Deficiency Diseases ◽

Cell Line Generation

B-lymphocytes normally synthesize small amounts of immunoglobulin, some of which is incorporated into the cell membrane where it serves as receptor of antigen. These cells, on contact with specific antigen, proliferate and differentiate to plasma cells which synthesize and secrete large quantities of immunoglobulin. The two stages of differentiation of this cell line (generation of B-lymphocytes and antigen-driven maturation to plasma cells) are clearly separable during ontogeny and in some immune deficiency diseases. The present report describes morphologic aberrations of B-lymphocytes in two diseases in which second stage differentiation is defective.

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