scholarly journals Effect of Biological and Biochemical Silage Additives on Final Nutritive, Hygienic and Fermentation Characteristics of Ensiled High Moisture Crimped Corn

2009 ◽  
Vol 78 (4) ◽  
pp. 691-698 ◽  
Author(s):  
Daniel Bíro ◽  
Branislav Gálik ◽  
Miroslav Juráček ◽  
Milan Šimko ◽  
Eva Straková ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Active Principle ◽  
High Moisture ◽  
Crude Fibre ◽  
Nitrogen Free Extract ◽  
Nutritive Quality ◽  
Experiment Control ◽  
Control Variant ◽  
Silage Additives

The aim of this study was to determine the effect of different biological and biochemical additives on the final nutritive quality, fermentation process and concentration of mycotoxins of ensiled high moisture crimped corn. We created four variants for the experiment: control (UC), A1, and A2 (biological stimulators with the active principle of lactic acid bacteria) and variant B (combined additives with the active principle of lactic acid bacteria, benzoate sodium and active enzymatic complex of cellulases). After 6 months of storage in laboratory conditions, we determined in experimental silages the content of dry matter ranging from 608.9 to 613 g kg-1. The significantly lower content of crude fibre was detected in silages with additives. In silages ensiled with additives we detected the highest content of nitrogen-free extract in variant B (834.3 g kg-1 of DM, P < 0.05). A similar effect was determined also in the content of starch; significant differences were detected in variants A1 and B (P < 0.05) compared to the control variant. We detected a significantly (P < 0.05) higher content of total sugars in trial silages; the highest content was in variant A2 (6.1 g kg-1 of DM). In the trial variants we determined significantly the lowest content of acetic acid in variant B (2.82 g kg-1 of DM). In case of butyric acid, whose content in the control variant was 0.22 g kg-1 of DM, we detected the lowest content in variant A1 conserved with homo- and heterofermentative species of lactic acid bacteria. The lowest content of ammonia was determined in silages of variant B (0.074 g kg-1 of DM). We found lower concentrations of DON and FUM (P > 0.05) after the application of biological and biochemical silage additives. In concentration of T-2 toxin we detected a significantly (P < 0.05) lower value in variant A1. In concentration of AFL we found significant differences between variants A1 and B, as well as in concentration of OT between untreated control variant (UC) and variants conserved by additives. Application of silage additives influenced the nutritive and hygienic quality of the conserved fodders.

scholarly journals QUALITY AND AEROBIC STABILITY OF FERMENTED ROLLED CORN GRAIN IN UNDER COMBINED INTRODUCTION OF PROBIOTIC STRAINS OF LACTIC ACID BACTERIA AND REPRESENTATIVES OF BACILLUS SUBTILIS

2019 ◽  
Vol 29 ◽  
pp. 62-70 ◽  
Author(s):  
M. G. Perederii ◽  
N. O. Kravchenko ◽  
L. V. Bozhok ◽  
O. M. Dmytruk
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Positive Control ◽  
Raw Material ◽  
Crude Fibre ◽  
Aerobic Stability ◽  
Probiotic Strains ◽  
Control Variant ◽  
Corn Grain

Objective. To find out the effect of combined introduction of probiotic strains of lactic acid bac-teria and Bacillus subtilis on the quality and aerobic stability of fermented rolled corn grain. Methods. Microbiological, zootechnical. Results. According to the results of organoleptic analysis, preserved rolled corn grain of the experimental and control variants had a preserved texture with a pleasant fruity odour. Preservation of crude fibre in the raw material of the experimental variants treated with Bacillus subtilis ВРT-B1 and under the combined use of lactic acid bacteria with aero-bic bacilli was higher by 10.0 % and 3.3 %, respectively, as compared to positive control parame-ters (Subtikon grain processing). The protein content in the experimental variants and the positive control was higher by 2.4–7.2 % than in the control variant of the feed (without the use of microor-ganisms). After aerobic exposure of feed for 14 days, preservation of crude fibre in all variants be-gan to decrease, however, the greatest losses (20 %) were noted in the control variant (without in-troduction of bacteria). The highest protein losses were observed in the control variant (3.8 %).After aerobic exposure, acidity in all variants of the experiment remained in the optimal range of pH 3.9–4.2. At day 70 of feed preservation, an increase in the number of lactic acid bacteria by 2–3 orders of magnitude in samples of experimental variants and positive control in comparison with this parameter in the raw material was noted. After air access to preserved rolled corn grain for 14 days, the lowest number of lactic acid bacteria was observed in absolute control, the high-est — in the variant with the combined inoculation by strains of Lactobacillus plantarum КT-L18/1 and Bacillus subtilis ВРT-B1. No pathogenic and butyric acid bacteria were detected. Conclusi-on. The combined use of probiotic strains of lactic acid bacteria and Bacillus subtilis to preserve corn grain contributes to the preservation of nutrients, the establishment and maintenance of an op-timal level of acidity, prevents aerobic food spoilage.

scholarly journals Dynamics of Fermentation Parameters and Bacterial Community in High-Moisture Alfalfa Silage with or without Lactic Acid Bacteria

2021 ◽  
Vol 9 (6) ◽  
pp. 1225
Author(s):  
Shanshan Zhao ◽  
Fengyuan Yang ◽  
Yuan Wang ◽  
Xiaomiao Fan ◽  
Changsong Feng ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacterial Community ◽  
Ph Value ◽  
Ammoniacal Nitrogen ◽  
High Concentration ◽  
High Moisture ◽  
Acid Accumulation ◽  
Alfalfa Silage ◽  
Fermentation Parameters

The aim of this study was to gain deeper insights into the dynamics of fermentation parameters and the bacterial community during the ensiling of high-moisture alfalfa. A commercial lactic acid bacteria (YX) inoculant was used as an additive. After 15 and 30 days of ensiling, the control silage (CK) exhibited a high pH and a high concentration of ammoniacal nitrogen (NH3-N); Enterobacter and Hafnia-Obesumbacterium were the dominant genera. At 60 d, the pH value and the concentration of NH3-N in CK silage increased compared with 15 and 30 d, propionic acid and butyric acid (BA) were detected, and Garciella had the highest abundance in the bacterial community. Compared with CK silage, inoculation of YX significantly promoted lactic acid and acetic acid accumulation and reduced pH and BA formation, did not significantly reduce the concentration of NH3-N except at 60 d, and significantly promoted the abundance of Lactobacillus and decreased the abundance of Garciella and Anaerosporobacter, but did not significantly inhibit the growth of Enterobacter and Hafnia-Obesumbacterium. In conclusion, high-moisture alfalfa naturally ensiled is prone to rot. Adding YX can delay the process of silage spoilage by inhibiting the growth of undesirable microorganisms to a certain extent.

The voluntary intake and digestibility by sheep of grass silages treated with different silage inoculants

1991 ◽  
Vol 1991 ◽  
pp. 82-82
Author(s):  
J A Rooke ◽  
F Kafilzadeh
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Live Weight ◽  
Random Order ◽  
Animal Performance ◽  
Silage Fermentation ◽  
Silage Inoculants ◽  
Silage Additives ◽  
Forage Harvester ◽  
Silage Inoculant

The selection of lactic acid bacteria for use as silage additives is normally based upon their ability to dominate the silage fermentation and not upon benefits in animal performance. The object of this study was to investigate whether two lactic acid bacteria selected for fermentation characteristics would support the same animal performance as an established silage inoculant.On 5 June 1989, first cut, predominantly perennial ryegrass (Lolium perenne) was ensiled direct with no wilting in plastolene silos of 2 tonne capacity. The herbage was harvested with a precision chop forage harvester and the following additive treatments were applied: None, control (C); Formic acid (Add-F, BP Nutrition, 850g/kg; 3 litres/tonne), (F); inoculant E. (Ecosyl, ICI Pic, 106Lactobacillus plantarum /g): inoculant A, (Pediococcus sp, 10 /g); inoculant B (L. plantarum 106 /g). The silages were fed to 6 wether sheep (Suffolk x Halfbred), initial live-weight, 40.1 kg (s.d. 2.22kg). Because the silages were unstable aerobically and restricted quantities of each silage were available, all sheep were fed the silages in the same (random) order.

An evaluation of the concentrate sparing effect of four silage additives

1992 ◽  
Vol 1992 ◽  
pp. 153-153
Author(s):  
C S Mayne
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Formic Acid ◽  
Animal Performance ◽  
Bacterial Inoculants ◽  
Milk Output ◽  
Sparing Effect ◽  
Silage Additives ◽  
Constant Yield ◽  
Potential Concentrate

Recent research at this Institute has shown marked improvements in animal performance following the use of bacterial inoculants as silage additives (Gordon, 1989 and Mayne, 1990). Other studies with additives based on antimicrobial carboxylic salts (Maxgrass - BP Chemicals) have also shown large improvements in animal performance relative to untreated silage (Chamberlain et al, 1990). However with constraints on milk output in the form of milk quotas, it is important to investigate the potential “concentrate sparing” effect of these differing silage additives, whilst producing a constant yield of milk constituents.Five silages with a range of fermentation characteristics were produced by ensiling first regrowth herbage either untreated (C) or treated with four differing silage additives including: an inoculant of lactic acid bacteria (I); an inoculant of lactic acid bacteria plus enzymes (I+E); formic acid applied at 2.54 litres/t grass (FA) and antimicrobial carboxylic acids applied at 5.95 litres/t grass (CA).

Varying ensiling conditions affect the fermentation quality and abundance of bacterial key players in lucerne silages

2020 ◽  
Vol 158 (4) ◽  
pp. 297-303
Author(s):  
T. Hartinger ◽  
K. Kube ◽  
N. Gresner ◽  
K.-H. Südekum
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Ammonia Nitrogen ◽  
Quality Characteristics ◽  
Lactic Acid Concentration ◽  
Spoilage Bacteria ◽  
Key Players ◽  
Silage Quality ◽  
Silage Additives

AbstractThe successful ensiling of lucerne (Medicago sativa L.) depends on a rapid acidification in the silo and consequently relies on a sufficient proliferation of, particularly homofermentative, lactic acid bacteria. Similarly, growth of spoilage bacteria, such as enterobacteria and clostridia, must be suppressed and silage additives are therefore frequently applied to promote favourable conditions during ensiling. Three silage additives or soil were applied during lucerne ensiling and investigated for their effects on silage quality characteristics and abundances of total bacteria as well as the bacterial key players Lactobacillus spp., homofermentative Lact. plantarum, heterofermentative Lact. buchneri, Clostridium spp. and Enterobacteriaceae after 30 days of storage. Inoculation with viable Lact. plantarum resulted in highest concentration of this species and excellent silage quality, i.e. high lactic acid concentration coupled with low acetic acid and ammonia-nitrogen concentrations. A sodium nitrite and hexamine-based additive did not support growth of lactic acid bacteria, which was also apparent by higher pH and low lactic acid concentration. No effect of treatments was found on spoilage-related enterobacteria and clostridia, even not when adding soil to lucerne to increase initial clostridial contamination. However, soil treatment resulted in increased ammonia-nitrogen and acetic acid concentrations. Consequently, among the bacterial key players, lactic acid bacteria concentrations were related to silage quality. Regarding spoilage bacteria, however, alterations in silage quality characteristics were not reflected in the abundances of enterobacteria and clostridia. Future investigations should underpin the present findings and help to understand how silage additives affect microbial key players and silage fermentation.

scholarly journals Isolation and characterization of lactic acid bacteria for use as silage additives

2016 ◽  
Vol 52 (4) ◽  
pp. 444-454 ◽  
Author(s):  
Yu-Mi Ro ◽  
Gwan-Hyeong Lee ◽  
InCheol Park ◽  
Wan-Gyu Kim ◽  
Byeong-Hak Han ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Isolation And Characterization ◽  
Silage Additives

Performance of finishing beef cattle fed diets containing maize silages inoculated with lactic-acid bacteria and Bacillus subtilis

2019 ◽  
Vol 59 (2) ◽  
pp. 266 ◽  
Author(s):  
Carlos H. S. Rabelo ◽  
André L. S. Valente ◽  
Rondineli P. Barbero ◽  
Fernanda C. Basso ◽  
R. A. Reis
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Beef Cattle ◽  
Growth Performance ◽  
Average Daily Gain ◽  
Protein Digestibility ◽  
Acid Detergent Fibre ◽  
Finishing Beef Cattle ◽  
Silage Additives

Our objective was to evaluate the effect of lactic-acid bacteria and Bacillus subtilis as silage additives on feed intake and growth performance of finishing feedlot beef cattle. Whole-maize forage was ensiled either with distilled water (untreated), or inoculated with Lactobacillus buchneri and L. plantarum at a rate 1 × 105 cfu/g fresh forage for each bacteria (LBLP); or inoculated with B. subtilis and L. plantarum at a rate 1 × 105 cfu/g fresh forage for each bacteria (BSLP). Thirty-six young crossbreed bulls (316 ± 33.9 kg) were used in the feedlot program for 110 days, and they were assigned (n = 12) to one of three diets containing untreated, LBLP, or BSLP silages in a 40:60 forage:concentrate ratio. Dry matter (DM) intake, average daily gain, and carcass yield of bulls were unaffected by silage inoculation. Conversely, bulls fed the BSLP silage had lower DM, organic matter, and crude protein digestibility compared with bulls fed untreated silage. Bulls fed both inoculated silages had a reduction of ~12% in neutral detergent fibre and acid detergent fibre digestibility compared with that in bulls fed untreated silage. Bulls fed the LBLP silage spent more time chewing (496 min/day) than bulls fed untreated silage. There was little effect of silage inoculation on rumen fermentation, but bulls fed the inoculated silages had a lower concentration of ammonia-N. In conclusion, adding L. plantarum combined with L. buchneri or B. subtilis to maize silage do not improve the growth performance of finishing feedlot beef cattle.

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