Lactic Acid Bacteria in Ensiled High-Moisture Corn Grain: Physiological and Genetic Characterization

1984 ◽  
Vol 5 (4) ◽  
pp. 534-544 ◽  
Author(s):  
Franco Dellaglio ◽  
Marisa Vescovo ◽  
Lorenzo Morelli ◽  
Sandra Torriani
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Genetic Characterization ◽  
High Moisture ◽  
Corn Grain ◽  
High Moisture Corn

scholarly journals Dynamics of Fermentation Parameters and Bacterial Community in High-Moisture Alfalfa Silage with or without Lactic Acid Bacteria

2021 ◽  
Vol 9 (6) ◽  
pp. 1225
Author(s):  
Shanshan Zhao ◽  
Fengyuan Yang ◽  
Yuan Wang ◽  
Xiaomiao Fan ◽  
Changsong Feng ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacterial Community ◽  
Ph Value ◽  
Ammoniacal Nitrogen ◽  
High Concentration ◽  
High Moisture ◽  
Acid Accumulation ◽  
Alfalfa Silage ◽  
Fermentation Parameters

The aim of this study was to gain deeper insights into the dynamics of fermentation parameters and the bacterial community during the ensiling of high-moisture alfalfa. A commercial lactic acid bacteria (YX) inoculant was used as an additive. After 15 and 30 days of ensiling, the control silage (CK) exhibited a high pH and a high concentration of ammoniacal nitrogen (NH3-N); Enterobacter and Hafnia-Obesumbacterium were the dominant genera. At 60 d, the pH value and the concentration of NH3-N in CK silage increased compared with 15 and 30 d, propionic acid and butyric acid (BA) were detected, and Garciella had the highest abundance in the bacterial community. Compared with CK silage, inoculation of YX significantly promoted lactic acid and acetic acid accumulation and reduced pH and BA formation, did not significantly reduce the concentration of NH3-N except at 60 d, and significantly promoted the abundance of Lactobacillus and decreased the abundance of Garciella and Anaerosporobacter, but did not significantly inhibit the growth of Enterobacter and Hafnia-Obesumbacterium. In conclusion, high-moisture alfalfa naturally ensiled is prone to rot. Adding YX can delay the process of silage spoilage by inhibiting the growth of undesirable microorganisms to a certain extent.

Rehydration of dry corn preserves the desirable bacterial community during ensiling

2020 ◽  
Vol 367 (17) ◽  
Author(s):  
Paula de Almeida Carvalho-Estrada ◽  
Pedro Avelino Maia de Andrade ◽  
Solidete de Fátima Paziani ◽  
Luiz Gustavo Nussio ◽  
Maria Carolina Quecine
Keyword(s):  
Bacterial Community ◽  
High Throughput Sequencing ◽  
Storage Period ◽  
Dry Matter Content ◽  
High Moisture ◽  
Corn Hybrids ◽  
Tropical Conditions ◽  
Corn Grain ◽  
Corn Grains ◽  
High Moisture Corn

ABSTRACT This study evaluated the rehydration approach of mature corn grains as an alternative for high-moisture corn grain silage production in distinct corn hybrids, storage period, cultivation locations and kernel maturity at plant harvest. High-moisture corn was used as a control. The dry matter content and pH of the silage were measured, and the bacterial community associated with corn grains pre- and post-ensiling was also assessed through 16S rRNA high-throughput sequencing. The decrease in pH value was directly linked to an ecological microbial succession of Enterobacteriales and Actinomycetales to Lactobacillales in the silage at 120 days after storage, either in rehydrated or high-moisture corn. These results were similar for both maize production locations and hybrids tested. Finally, the similarity between the ensiling processes including rehydrated corn and the high-moisture corn grain silages proves the reliability of the rehydration approach as an alternative for the maintenance of a successful bacterial community structure and composition capable of producing high-quality silages from dent and flint corn hybrids in tropical conditions.

scholarly journals Effect of Biological and Biochemical Silage Additives on Final Nutritive, Hygienic and Fermentation Characteristics of Ensiled High Moisture Crimped Corn

2009 ◽  
Vol 78 (4) ◽  
pp. 691-698 ◽  
Author(s):  
Daniel Bíro ◽  
Branislav Gálik ◽  
Miroslav Juráček ◽  
Milan Šimko ◽  
Eva Straková ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Active Principle ◽  
High Moisture ◽  
Crude Fibre ◽  
Nitrogen Free Extract ◽  
Nutritive Quality ◽  
Experiment Control ◽  
Control Variant ◽  
Silage Additives

The aim of this study was to determine the effect of different biological and biochemical additives on the final nutritive quality, fermentation process and concentration of mycotoxins of ensiled high moisture crimped corn. We created four variants for the experiment: control (UC), A1, and A2 (biological stimulators with the active principle of lactic acid bacteria) and variant B (combined additives with the active principle of lactic acid bacteria, benzoate sodium and active enzymatic complex of cellulases). After 6 months of storage in laboratory conditions, we determined in experimental silages the content of dry matter ranging from 608.9 to 613 g kg-1. The significantly lower content of crude fibre was detected in silages with additives. In silages ensiled with additives we detected the highest content of nitrogen-free extract in variant B (834.3 g kg-1 of DM, P < 0.05). A similar effect was determined also in the content of starch; significant differences were detected in variants A1 and B (P < 0.05) compared to the control variant. We detected a significantly (P < 0.05) higher content of total sugars in trial silages; the highest content was in variant A2 (6.1 g kg-1 of DM). In the trial variants we determined significantly the lowest content of acetic acid in variant B (2.82 g kg-1 of DM). In case of butyric acid, whose content in the control variant was 0.22 g kg-1 of DM, we detected the lowest content in variant A1 conserved with homo- and heterofermentative species of lactic acid bacteria. The lowest content of ammonia was determined in silages of variant B (0.074 g kg-1 of DM). We found lower concentrations of DON and FUM (P > 0.05) after the application of biological and biochemical silage additives. In concentration of T-2 toxin we detected a significantly (P < 0.05) lower value in variant A1. In concentration of AFL we found significant differences between variants A1 and B, as well as in concentration of OT between untreated control variant (UC) and variants conserved by additives. Application of silage additives influenced the nutritive and hygienic quality of the conserved fodders.

scholarly journals The effect of combinations of dried or ensiled corn and sorghum on the performance of feedlot lambs

2019 ◽  
Vol 40 (4) ◽  
pp. 1543
Author(s):  
Marina Gabriela Berchiol Silva ◽  
Daniele Floriano Fachiolli ◽  
Ciniro Costa ◽  
Paulo Roberto de Lima Meirelles ◽  
Marco Aurélio Factori ◽  
...  
Keyword(s):  
Experimental Design ◽  
Carcass Weight ◽  
Meat Production ◽  
Average Weight ◽  
High Moisture ◽  
Feedlot Lambs ◽  
Sorghum Grain ◽  
Corn Grain ◽  
High Moisture Corn

The objective of this study was to evaluate the effects of combinations of dry and moist corn and sorghum grain on the carcass and cut characteristics of crossbred lambs Suffolk x Santa Inês produced in feedlots. The experimental design was a randomized block with four treatments and three replicates. Sixty uncastrated male lambs with an initial average weight of 15.5 kg were used in this experiment. Four combinations of grain were evaluated: dry sorghum milled + silage of high-moisture sorghum grain (DS: MS); dry corn milled + silage of high-moisture sorghum grain (DC:MS); dry corn milled + silage of high-moisture corn grain (DC:MC); dry sorghum milled + silage of high-moisture corn grain (DS:MC). There was no difference between treatments for carcass yield, warm and cold carcass weight and for cooling loss. The slaughtering weights of lambs fed DC:MS associations were higher (29.46 kg) than lambs fed DC: MC (24.17 kg), but both did not differ DS:MS (26.18 kg) and DS:MC (25.21 kg). There was influence of the grain associations on the yield of commercial cuts palette and shank. Combinations of corn and sorghum grain, dried and ensiled, can be used to feed lambs in confinement for meat production without affecting the quality of the carcass.

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